Влияние функциональной взаимосвязи понятия, образа и действия на процесс решения междисциплинарных технологически ориентированных задач

This article studies the influence of the methods used by students to solve tasks on the effectiveness of the learning process based on their analysis. The study has revealed that mastering the combined method based on the functional relationship between concept, image and action in the process of solving interdisciplinary technological tasks is one of the mechanisms of effective preparation of students for professional activity in the labour market of related professions.En este artículo, basado en un análisis de los métodos utilizados por los estudiantes para resolver problemas, se investiga su influencia en la efectividad del proceso de aprendizaje. En el curso del estudio, se reveló que el dominio del método combinado, basado en la relación funcional del concepto, la imagen y la acción, en el proceso de resolución de problemas tecnológicos interdisciplinarios es uno de los mecanismos para la preparación efectiva de los estudiantes para actividades profesionales en el contexto de la aparición de profesiones relacionadas en el mercado laboral.В данной статье на основе анализа используемых обучающимися способов решения задач исследуется их влияние на результативность процесса обучения. В процессе исследования выявлено, что владение комбинированным способом, основанном на функциональной взаимосвязи понятия, образа и действия, в процессе решения междисциплинарных технологических задач выступает одним из механизмов эффективной подготовки обучающихся к профессиональной деятельности в условиях появления на рынке труда смежных профессий

Systematic comparison of sea urchin and sea star developmental gene regulatory networks explains how novelty is incorporated in early development.

The extensive array of morphological diversity among animal taxa represents the product of millions of years of evolution. Morphology is the output of development, therefore phenotypic evolution arises from changes to the topology of the gene regulatory networks (GRNs) that control the highly coordinated process of embryogenesis. A particular challenge in understanding the origins of animal diversity lies in determining how GRNs incorporate novelty while preserving the overall stability of the network, and hence, embryonic viability. Here we assemble a comprehensive GRN for endomesoderm specification in the sea star from zygote through gastrulation that corresponds to the GRN for sea urchin development of equivalent territories and stages. Comparison of the GRNs identifies how novelty is incorporated in early development. We show how the GRN is resilient to the introduction of a transcription factor, pmar1, the inclusion of which leads to a switch between two stable modes of Delta-Notch signaling. Signaling pathways can function in multiple modes and we propose that GRN changes that lead to switches between modes may be a common evolutionary mechanism for changes in embryogenesis. Our data additionally proposes a model in which evolutionarily conserved network motifs, or kernels, may function throughout development to stabilize these signaling transitions

Slow-binding inhibition of acetylcholinesterase by an alkylammonium derivative of 6-methyluracil: Mechanism and possible advantages for myasthenia gravis treatment

© 2016 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society.Inhibition of human AChE (acetylcholinesterase) and BChE (butyrylcholinesterase) by an alkylammonium derivative of 6-methyluracil, C-547, a potential drug for the treatment of MG (myasthenia gravis) was studied. Kinetic analysis of AChE inhibition showed that C-547 is a slow-binding inhibitor of type B, i.e. after formation of the initial enzyme•inhibitor complex (Ki = 140 pM), an induced-fit step allows establishment of the final complex (Ki = 22 pM). The estimated koff is low, 0.05 -1 . On the other hand, reversible inhibition of human BChE is a fast-binding process of mixed-type (Ki = 1.77 μM; Ki = 3.17 μM). The crystal structure of mouse AChE complexed with C-547 was solved at 3.13 Å resolution. The complex is stabilized by cation-π , stacking and hydrogenbonding interactions. Molecular dynamics simulations of the binding/dissociation processes of C-547 and C-35 (a noncharged analogue) to mouse and human AChEs were performed. Molecular modelling on mouse and human AChE showed that the slow step results from an enzyme conformational change that allows C-547 to cross the bottleneck in the active-site gorge, followed by formation of tight complex, as observed in the crystal structure. In contrast, the related non-charged compound C-35 is not a slow-binding inhibitor. It does not cross the bottleneck because it is not sensitive to the electrostatic driving force to reach the bottom of the gorge. Thus C-547 is one of the most potent and selective reversible inhibitors of AChE with a long residence time, τ; = 20 min, longer than for other reversible inhibitors used in the treatment of MG. This makes C-547 a promising drug for the treatment of this disease

PNL in the treatment of staghorn nephrolithiasis

Staghorn nephrolithiasis most severe course of urolithiasis. A retrospective analysis of a group of patients with staghorn nephrolithiasis (n = 50), operated by various methods, followed by determination of the optimal method of surgery. Efficacy was evaluated by the presence of residual stones, the presence of postoperative complications. All patients were divided into 4 groups according to the stage of staghorn nephrolithiasis. In 20% of identified staghorn nephrolithiasis stage 1,40% - the second, at 8% - third, 20% - fourth. 60% was performed percutaneous nephrolitholapaxy (nephrolithotripsy), 14% - pyelolithotomy, 6% - nephrolithotomy, 4% extracorporeal shock wave lithotripsy was performed. Nephrectomies were performed in 12% of cases. The effectiveness of percutaneous nephrolithotripsy comparable to open surgery, and the number of postoperative complications is minimal, less than the time of hospital treatment and postoperative rehabilitation.Коралловидный нефролитиаз наиболее тяжелое течение мочекаменной болезни. Проведен анализ группы больных с корраловидным нефропитиазом (КН) (п=50), оперированных различными методиками с последующим определением оптимального метода хирургического вмешательства. Эффективность оценивалась по наличию резидуальных камней, наличию послеоперационных осложнений. Все пациенты были разделены на 4 группы по стадии КН. В 20% выявлен КН 1 стадии, у 40 % - второй, в 8% - третей и 20% - четвертой. 60% была выполнена чрескожная нефролитолапаксия (нефролитотрипсия), 14 % - пиелолитотомия, 6 % - нефролитотомия и 4% была проведена дистанционная литотрипсия. Органуносящие операции выполнялись в 12 % случаев. Эффективность чрескожной нефролитотрипсии сопоставима с открытым оперативным вмешательством, а количество послеоперационных осложнений минимально, меньше сроки стационарного лечения и послеоперационной реабилитации

Whole genome SNP-associated signatures of local adaptation in honeybees of the Iberian Peninsula

The availability of powerful high-throughput genomic tools, combined with genome scans, has helped identifying genes and genetic changes responsible for environmental adaptation in many organisms, including the honeybee. Here, we resequenced 87 whole genomes of the honeybee native to Iberia and used conceptually different selection methods (Samβada, LFMM, PCAdapt, iHs) together with in sillico protein modelling to search for selection footprints along environmental gradients. We found 670 outlier SNPs, most of which associated with precipitation, longitude and latitude. Over 88.7% SNPs laid outside exons and there was a significant enrichment in regions adjacent to exons and UTRs. Enrichment was also detected in exonic regions. Furthermore, in silico protein modelling suggests that several non-synonymous SNPs are likely direct targets of selection, as they lead to amino acid replacements in functionally important sites of proteins. We identified genomic signatures of local adaptation in 140 genes, many of which are putatively implicated in fitness-related functions such as reproduction, immunity, olfaction, lipid biosynthesis and circadian clock. Our genome scan suggests that local adaptation in the Iberian honeybee involves variations in regions that might alter patterns of gene expression and in protein-coding genes, which are promising candidates to underpin adaptive change in the honeybee.John C. Patton, Phillip San Miguel, Paul Parker, Rick Westerman, University of Purdue, resequenced the 87 whole genomes of IHBs. Jose Rufino provided computational resources at IPB. Analyses were performed using the computational resources at the Uppsala Multidisciplinary Center for Advanced Computational Science (UPPMAX), Uppsala University. DH was supported by a PhD scholarship (SFRH/BD/84195/2012) from the Portuguese Science Foundation (FCT). MAP is a member of and receives support from the COST Action FA1307 (SUPER-B). This work was supported by FCT through the programs COMPETE/QREN/EU (PTDC/BIA-BEC/099640/2008) and the 2013-2014 BiodivERsA/FACCE-JPI (joint call for research proposals, with the national funders FCT, Portugal, CNRS, France, and MEC, Spain) to MAP

Very slow surface plasmons: theory and practice

The paper is of a methodological character and has as a goal to give a brief description of the concept of theory and practical application of very slow optical plasmons. They exist on the metal-dielectric boundaries, namely, on very thin metal films and fibers and as standing waves on metal spheres and ellipsoids. The material presented in the paper features by widening the common concepts of electromagnetic modes of various spaces, of the probability of spontaneous emission, of creation of optical images, of the limits of optical focusing, and of the photon linear momentum. All mentioned studies are completed in recent years. The problem of the photon momentum in a dielectric medium was the topic of irreconcilable disputes for 100 years starting in the time of appearing of Minkowski and Abraham famous papers. Various practical applications are surveyed: the experiments with a great intensification of an atom spontaneous emission into a plasmonic field mode of a metal nanoparticle, the experiments on focusing optical radiation into a spot that substantially smaller than a diffraction limited spot, a so called near perfect Pendry lens that produces the image with details that substantially smaller than defined by diffraction, and lastly, the concept of hundredfold and more magnification of a photon mechanical linear momentum in a plasmon. The work completed is supported by RFBR, grants Nos 05-02-19647, 07-02-01328.Comment: 19 pages, 13 figure