18,946 research outputs found
DNA-Mediated Electrochemistry
The base pair stack of DNA has been demonstrated as a medium for long-range charge transport chemistry both in solution and at DNA-modified surfaces. This chemistry is exquisitely sensitive to structural perturbations in the base pair stack as occur with lesions, single base mismatches, and protein binding. We have exploited this sensitivity for the development of reliable electrochemical assays based on DNA charge transport at self-assembled DNA monolayers. Here, we discuss the characteristic features, applications, and advantages of DNA-mediated electrochemistry
Constitutive Association of Tie1 and Tie2 with Endothelial Integrins is Functionally Modulated by Angiopoietin-1 and Fibronectin
Functional cross-talk between Tie2 and Integrin signaling pathways is essential to coordinate endothelial cell adhesion and migration in response to the extracellular matrix, yet the mechanisms behind this phenomenon are unclear. Here, we examine the possibility that receptor cross-talk is driven through uncharacterized Tie-integrin interactions on the endothelial surface. Using a live cell FRET-based proximity assay, we monitor Tie-integrin receptor recognition and demonstrate that both Tie1 and Tie2 readily associate with integrins α5ß1 and αVß3 through their respective ectodomains. Although not required, Tie2-integrin association is significantly enhanced in the presence of the extracellular component and integrin ligand fibronectin. In vitro binding assays with purified components reveal that Tie-integrin recognition is direct, and further demonstrate that the receptor binding domain of the Tie2 ligand Ang-1, but not the receptor binding domain of Ang-2, can independently associate with α5ß1 or αVß3. Finally, we reveal that cooperative Tie/integrin interactions selectively stimulate ERK/MAPK signaling in the presence of both Ang-1 and fibronectin, suggesting a molecular mechanism to sensitize Tie2 to extracellular matrix. We provide a mechanistic model highlighting the role of receptor localization and association in regulating distinct signaling cascades and in turn, the angiogenic switch
Tunnel switch diode based on AlSb/GaSb heterojunctions
We report on tunnel switch diodes based on AlSb barriers and GaSb p–n junctions grown by molecular beam epitaxy. These were the devices with thyristor like switching in the GaSb/AlSb system. The characteristic "S" shaped current–voltage curve was found to occur for structures with AlSb barriers less than 300 Å thick. The switching voltage and current density exhibited less sensitivity to barrier and epilayer thickness than was predicted by the punch-through model. The results were correlated with drift diffusion simulations which have been modified to account for the presence of a tunneling contact
Computer program to predict noise of general aviation aircraft: User's guide
Program NOISE predicts General Aviation Aircraft far-field noise levels at FAA FAR Part 36 certification conditions. It will also predict near-field and cabin noise levels for turboprop aircraft and static engine component far-field noise levels
Optimal Computation of Avoided Words
The deviation of the observed frequency of a word from its expected
frequency in a given sequence is used to determine whether or not the word
is avoided. This concept is particularly useful in DNA linguistic analysis. The
value of the standard deviation of , denoted by , effectively
characterises the extent of a word by its edge contrast in the context in which
it occurs. A word of length is a -avoided word in if
, for a given threshold . Notice that such a word
may be completely absent from . Hence computing all such words na\"{\i}vely
can be a very time-consuming procedure, in particular for large . In this
article, we propose an -time and -space algorithm to compute all
-avoided words of length in a given sequence of length over a
fixed-sized alphabet. We also present a time-optimal -time and
-space algorithm to compute all -avoided words (of any
length) in a sequence of length over an alphabet of size .
Furthermore, we provide a tight asymptotic upper bound for the number of
-avoided words and the expected length of the longest one. We make
available an open-source implementation of our algorithm. Experimental results,
using both real and synthetic data, show the efficiency of our implementation
Theoretical studies of Si and GaAs surfaces and initial steps in the oxidation
Using ab initio quantum chemical methods (generalized valence bond), we examine (i) the electronic states of Si (111) and GaAs (110) surface, (ii) the relaxation of the Si (111) surface, (iii) the reconstruction of the GaAs surface, (iv) the initial steps in the chemisorption of O_2 on Si (111), and (v) the bonding of O atom to Ga and As centers
Low-background temperature sensors fabricated on parylene substrates
Temperature sensors fabricated from ultra-low radioactivity materials have
been developed for low-background experiments searching for neutrinoless
double-beta decay and the interactions of WIMP dark matter. The sensors consist
of electrical traces photolithographically-patterned onto substrates of
vapor-deposited parylene. They are demonstrated to function as expected, to do
so reliably and robustly, and to be highly radio-pure. This work is a
proof-of-concept study of a technology that can be applied to broad class of
electronic circuits used in low-background experiments
The geography of strain: organizational resilience as a function of intergroup relations
Organizational resilience is an organization’s ability to absorb strain and preserve or
improve functioning, despite the presence of adversity. In existing scholarship there is
the implicit assumption that organizations experience and respond holistically to acute
forms of adversity. We challenge this assumption by theorizing about how adversity can
create differential strain, affecting parts of an organization rather than the whole. We
argue that relations among those parts fundamentally shape organizational resilience.
We develop a theoretical model that maps how the differentiated emergence of strain in
focal parts of an organization triggers the movements of adjoining parts to provide or
withhold resources necessary for the focal parts to adapt effectively. Drawing on core
principles of theories about intergroup relations, we theorize about three specific
pathways—integration, disavowal, and reclamation—by which responses of adjoining
parts to focal part strain shape organizational resilience. We further theorize about
influences on whether and when adjoining parts are likely to select different pathways.
The resulting theory reveals how the social processes among parts of organizations
influence member responses to adversity and, ultimately, organizational resilience. We
conclude by noting the implications for organizational resilience theory, research, and
practice.Accepted manuscrip
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