1,619 research outputs found
Inhibition of monocyte complement receptor enhancement by low molecular weight material from human lung cancers
We have studied the effect of dialysates from lung cancer homogenates to alter both the expression of complement (C3b) receptors per se and also to inhibit leucoattractant-induced enhancement of complement rosettes on monocytes from healthy individuals. Enhancement and enhancement-inhibition by tumour extracts were compared with material derived from normal lung excised from distance from the tumour. There was no significant difference between tumour homogenate (TH) and normal lung homogenate (NLH) in terms of enhancement of complement rosettes per se. In contrast, TH produced a dose- and time-dependent inhibition of leucoattractant-induced enhancement of C3b rosettes which was significantly different from that obtained with NLH. This enhancement-inhibition was observed with four undifferentiated, four squamous and three adenocarcinomas of lung. The degree of enhancement-inhibition was not related to the type of tumour or varying accompanying histological features such as necrosis and the degree of infiltration with inflammatory cells. Following gel filtration on Sephadex G-50 each type of cancer gave a major peak of inhibitory activity which eluted with molecules having an apparent molecular size of approximately 3,000 daltons. A second larger peak (8,000-10,000 daltons) was also detected with extracts from the undifferentiated and adenocarcinomas. These results support previous findings, mainly from experimental animals, indicating that 'anti-macrophage/monocyte principles' are elaborated from certain tumour types
The stellar content of brightest cluster galaxies
We present near-infrared K-band spectroscopy of 21 elliptical or cD Brightest
Cluster Galaxies (BCGs), for which we have measured the strength of the 2.293
micron CO stellar absorption feature. We find that the strength of this feature
is remarkably uniform among these galaxies, with a smaller scatter in
equivalent width than for the normal elliptical population in the field or
clusters. The scatter for BCGs is 0.156 nm, compared with 0.240 nm for Coma
cluster ellipticals, 0.337 nm for ellipticals from a variety of other clusters,
and 0.422 nm for field ellipticals. We interpret this homogeneity as being due
to a greater age, or more uniform history, of star formation in BCGs than in
other ellipticals; only a small fraction of the scatter can be due to
metallicity variations, even in the BCGs. Notwithstanding the small scatter,
correlations are found between CO strength and various galaxy properties,
including R-band absolute magnitude, which could improve the precision of these
galaxies as distance indicators in measurements of cosmological parameters and
velocity flows.Comment: 7 pages, 8 figures, accepted for publication by MNRA
River Restoration in the Twenty-First Century: Data and Experiential Knowledge to Inform Future Efforts
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/71999/1/j.1526-100X.2007.00243.x.pd
An organic fluorophore-nanodiamond hybrid sensor for photostable imaging and orthogonal, on-demand biosensing
Organic fluorescent probes are widely used to detect key biomolecules; however, they often lack the photostability required for extended intracellular imaging. Here we report a new hybrid nanomaterial (peroxynanosensor, PNS), consisting of an organic fluorescent probe bound to a nanodiamond, that overcomes this limitation to allow concurrent and extended cell-based imaging of the nanodiamond and ratiometric detection of hydrogen peroxide. Far-red fluorescence of the nanodiamond offers continuous monitoring without photobleaching, while the green fluorescence of the organic fluorescent probe attached to the nanodiamond surface detects hydrogen peroxide on demand. PNS detects basal production of hydrogen peroxide within M1 polarised macrophages and does not affect macrophage growth during prolonged co-incubation. This nanosensor can be used for extended bio-imaging not previously possible with an organic fluorescent probe, and is spectrally compatible with both Hoechst 33342 and MitoTracker Orange stains for hyperspectral imaging.Malcolm S. Purdey, Patrick K. Capon, Benjamin J. Pullen, Philipp Reineck,
Nisha Schwarz, Peter J. Psaltis, Stephen J. Nicholls, Brant C. Gibson and
Andrew D. Abel
NASA's Asteroid Redirect Mission: The Boulder Capture Option
NASA is examining two options for the Asteroid Redirect Mission (ARM), which will return asteroid material to a Lunar Distant Retrograde Orbit (LDRO) using a robotic solar-electric-propulsion spacecraft, called the Asteroid Redirect Vehicle (ARV). Once the ARV places the asteroid material into the LDRO, a piloted mission will rendezvous and dock with the ARV. After docking, astronauts will conduct two extravehicular activities (EVAs) to inspect and sample the asteroid material before returning to Earth. One option involves capturing an entire small (approximately 4-10 m diameter) near-Earth asteroid (NEA) inside a large inflatable bag. However, NASA is examining another option that entails retrieving a boulder (approximately 1-5 m) via robotic manipulators from the surface of a larger (approximately 100+ m) pre-characterized NEA. This option can leverage robotic mission data to help ensure success by targeting previously (or soon to be) well-characterized NEAs. For example, the data from the Hayabusa mission has been utilized to develop detailed mission designs that assess options and risks associated with proximity and surface operations. Hayabusa's target NEA, Itokawa, has been identified as a valid target and is known to possess hundreds of appropriately sized boulders on its surface. Further robotic characterization of additional NEAs (e.g., Bennu and 1999 JU3) by NASA's OSIRIS REx and JAXA's Hayabusa 2 missions is planned to begin in 2018. The boulder option is an extremely large samplereturn mission with the prospect of bringing back many tons of wellcharacterized asteroid material to the EarthMoon system. The candidate boulder from the target NEA can be selected based on inputs from the worldwide science community, ensuring that the most scientifically interesting boulder be returned for subsequent sampling. This boulder option for NASA's ARM can leverage knowledge of previously characterized NEAs from prior robotic missions, which provides more certainty of the target NEA's physical characteristics and reduces mission risk. This increases the return on investment for NASA's future activities with respect to science, human exploration, resource utilization, and planetary defens
Successful use of axonal transport for drug delivery by synthetic molecular vehicles
We report the use of axonal transport to achieve intraneural drug delivery. We constructed a novel tripartite complex of an axonal transport facilitator conjugated to a linker molecule bearing up to a hundred reversibly attached drug molecules. The complex efficiently enters nerve terminals after intramuscular or intradermal administration and travels within axonal processes to neuron cell bodies. The tripartite agent provided 100-fold amplification of saturable neural uptake events, delivering multiple drug molecules per complex. _In vivo_, analgesic drug delivery to systemic and to non-targeted neural tissues was greatly reduced compared to existing routes of administration, thus exemplifying the possibility of specific nerve root targeting and effectively increasing the potency of the candidate drug gabapentin 300-fold relative to oral administration
Microstructured optical fiber-based biosensors: reversible and nanoliter-scale measurement of zinc ions
Published: May 6, 2016Sensing platforms that allow rapid and efficient detection of metal ions would have applications in disease diagnosis and study, as well as environmental sensing. Here, we report the first microstructured optical fiber-based biosensor for the reversible and nanoliter-scale measurement of metal ions. Specifically, a photoswitchable spiropyran Zn(2+) sensor is incorporated within the microenvironment of a liposome attached to microstructured optical fibers (exposed-core and suspended-core microstructured optical fibers). Both fiber-based platforms retains high selectivity of ion binding associated with a small molecule sensor, while also allowing nanoliter volume sampling and on/off switching. We have demonstrated that multiple measurements can be made on a single sample without the need to change the sensor. The ability of the new sensing platform to sense Zn(2+) in pleural lavage and nasopharynx of mice was compared to that of established ion sensing methodologies such as inductively coupled plasma mass spectrometry (ICP-MS) and a commercially available fluorophore (Fluozin-3), where the optical-fiber-based sensor provides a significant advantage in that it allows the use of nanoliter (nL) sampling when compared to ICP-MS (mL) and FluoZin-3 (μL). This work paves the way to a generic approach for developing surface-based ion sensors using a range of sensor molecules, which can be attached to a surface without the need for its chemical modification and presents an opportunity for the development of new and highly specific ion sensors for real time sensing applications.Sabrina Heng, Christopher A. McDevitt, Roman Kostecki, Jacqueline R. Morey, Bart A. Eijkelkamp, Heike Ebendorff-Heidepriem, Tanya M. Monro, and Andrew D. Abel
Correlation length of X-ray brightest Abell clusters
We compute the cluster auto-correlation function of an X-ray
flux limited sample of Abell clusters (XBACs, \cite{ebe}). For the total XBACs
sample we find a power-law fit with Mpc
hand consistent with the results of Abell
clusters. We also analyze for subsamples defined by different
X-ray luminosity thresholds where we find a weak tendency of larger values of
with increasing X-ray luminosity although with a low statistical
significance. In the different subsamples analyzed we find Mpc
h and . Our analysis suggests that cluster X-ray
luminosities may be used for a reliable confrontation of cluster spatial
distribution properties in models and observations.Comment: Accepted for publication in Astrophysical Journa
Modeling of Covalent Bonding in Solids by Inversion of Cohesive Energy Curves
We provide a systematic test of empirical theories of covalent bonding in
solids using an exact procedure to invert ab initio cohesive energy curves. By
considering multiple structures of the same material, it is possible for the
first time to test competing angular functions, expose inconsistencies in the
basic assumption of a cluster expansion, and extract general features of
covalent bonding. We test our methods on silicon, and provide the direct
evidence that the Tersoff-type bond order formalism correctly describes
coordination dependence. For bond-bending forces, we obtain skewed angular
functions that favor small angles, unlike existing models. As a
proof-of-principle demonstration, we derive a Si interatomic potential which
exhibits comparable accuracy to existing models.Comment: 4 pages revtex (twocolumn, psfig), 3 figures. Title and some wording
(but no content) changed since original submission on 24 April 199
Separating the BL Lac and Cluster X-ray Emissions in Abell 689 with Chandra
We present the results of a Chandra observation of the galaxy cluster Abell
689 (z=0.279). Abell 689 is one of the most luminous clusters detected in the
ROSAT All Sky Survey (RASS), but was flagged as possibly including significant
point source contamination. The small PSF of the Chandra telescope allows us to
confirm this and separate the point source from the extended cluster X-ray
emission. For the cluster we determine a bolometric luminosity of
L_{bol}=(3.3+/-0.3)x10^{44} erg s-1 and a temperature of kT=5.1^{+2.2}_{-1.3}
keV when including a physically motivated background model. We compare our
measured luminosity for A689 to that quoted in the Rosat All Sky Survey (RASS)
and find L_{0.1-2.4,keV}=2.8x10^{44} erg s-1, a value \sim10 times lower than
the ROSAT measurement. Our analysis of the point source shows evidence for
significant pileup, with a pile-up fraction of ~60%. SDSS spectra and HST
images lead us to the conclusion that the point source within Abell 689 is a BL
Lac object. Using radio and optical observations from the VLA and HST archives,
we determine {\alpha}_{ro}=0.50, {\alpha}_{ox}=0.77 and {\alpha}_{rx}=0.58 for
the BL Lac, which would classify it as being of 'High-energy peak BL Lac' (HBL)
type. Spectra extracted of A689 show a hard X-ray excess at energies above 6
keV that we interpret as inverse Compton emission from aged electrons that may
have been transported into the cluster from the BL Lac.Comment: 11 pages, 15 figures, MNRAS in pres
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