Resistance of Staphylococcus aureus Recovered from Infected Foreign Body In Vivo to Killing by Antimicrobials

Because persistence of infections associated with prosthetic material despite the use of appropriate antibioticsis a major clinical problem,the antimicrobial susceptibility of bacteria responsible for a chronic subcutaneous tissue cage infection in rat was investigated ex vivo. Three to 6 weeks after the initiation of infection, suspensions of two strains of Staphylococcus aureus recovered from the foreign body surface and surrounding fluid wereexposed to either oxacillin, vancomycin, fleroxacin, gentamicin, or rifampin. The MBCs of these bacteria were markedly elevated, in most cases 128 to >256 times higher than the MBCof batch culture S. aureus in either logarithmic or stationary phase. Kinetic studies showed the bacteria did not growwhen incubated for 2 h in Mueller-Hinton broth, possibly reflecting dormancy. Their killing wasslow and incompleteby all antibioticsat > 8 times their MIC. These data provide direct evidence of a decreased susceptibility of S. aureus to the killing effect of antimicrobials during chronic foreign body infections in viv

Fibronectin, Fibrinogen, and Laminin Act as Mediators of Adherence of Clinical Staphylococcal Isolates to Foreign Material

Bacterial adherence to polymer surfaces is a required early step in intravenous (iv) device infection. We collected eight strains of Staphylococcus aureus and 19 of coagulase-negative staphylococci from patients with proven iv device bacteremia and studied the role of plasma or connective-tissue proteins in promoting bacterial adherence to polymethylmethacrylate (PMMA) coverslips. Although only a negligible percentage of organisms adhered to albumin-coated PMMA, surface-bound fibronectin significantly promoted adherence of all isolates. Fibrinogen markedly promoted adherence of all S. aureus strains but of only four coagulase-negative strains. Thus, coagulase-negative staphylococci revealed a marked heterogeneity in adherence to fibrinogen-coated surfaces, a result suggesting the existence of heretofore unknown receptors for fibrinogen. Laminin promoted adherence of staphylococci to a much lower extent. Although strain specific, adherence of clinical staphylococcal isolates to foreign surfaces is significantly increased by fibronectin, fibrinogen, and laminin, an observation suggesting the possible contribution of these proteins to the pathogenesis of iv device infectio

A Novel Approach to Combining Client-Dependent and Confidence Information in Multimodal Biometric

The issues of fusion with client-dependent and confidence information have been well studied separately in biometric authentication. In this study, we propose to take advantage of both sources of information in a discriminative framework. Initially, each source of information is processed on a per expert basis (plus on a per client basis for the first information and on a per example basis for the second information). Then, both sources of information are combined using a second-level classifier, across different experts. Although the formulation of such two-step solution is not new, the novelty lies in the way the sources of prior knowledge are incorporated prior to fusion using the second-level classifier. Because these two sources of information are of very different nature, one often needs to devise special algorithms to combine both information sources. Our framework that we call ``Prior Knowledge Incorporation'' has the advantage of using the standard machine learning algorithms. Based on $10 \times 32=320$ intramodal and multimodal fusion experiments carried out on the publicly available XM2VTS score-level fusion benchmark database, it is found that the generalisation performance of combining both information sources improves over using either or none of them, thus achieving a new state-of-the-art performance on this database

Susceptibility testing of Haemophilus influenzae— an international collaborative study in quality assessment

In order to compare the prevalence of antibiotic resistance in different geographical areas, it is necessary to ensure that agreement is achieved between laboratories on the assignment of strains to ‘susceptible' and ‘resistant' categories. An international quality assessment study, involving 15 laboratories in eight countries, was performed to investigate the standard of performance of the susceptibility testing of Haemophilus influenzae. One hundred and fifty strains of H. influenzae were distributed from the London Hospital Medical College (LHMC) to all laboratories who were asked to test the susceptibility of the strains to ampicillin, chloramphenicol, tetracycline, trimethoprim, cephalosporins and ciprofloxacin. Laboratories were also asked to provide the details of methodology to test the susceptibility. Significant discrepancy between the LHMC and the participating laboratories appeared in the detection of resistance to ampicillin (especially β-lactamase-negative strains resistant to ampicillin) as well as the assignment of susceptibility and resistance to chloramphenicol, tetracycline and trimethoprim. Often these reflected the use of inappropriate breakpoints which led to erroneous assignment of susceptibility. Other variations including disc content, medium and supplement, inoculum as well as failure to measure zone sizes properly also led to some repeating anomalie

Particle transport in a karst aquifer : natural and artifical tracer experiments with bacteria, bacteriophages and microspheres

Fast changes in spring water quality in karst areas are a major concern for production of drinking water and require detailed knowledge of the complex interaction between karst aquifer, transport behavior of microorganisms and water treatment We have conducted artificial and natural particle transport experiments at a karst spring with bacteria, bacteriophages, microspheres, and pathogens Transport of the investigated microorganisms, turbid matter and chemical pullutants as well as increase in discharge are strongly related to precipitation and the heterogeneity of the aquifer The indicator bacteria E cob revealed a significant correlation to verotoxin-producing E cob and Cryptosporidium spp We conclude that artificial particle tracers can help identify "hot spots" for microbial recharge and that system parameters in spring water such as turbidity, UV-extinction and increase in discharge can be key parameters for efficient raw water management