Individual differences in growth and in accumulation of secondary metabolites in Rhodiola rosea cultivated in Western Siberia

In this study, growth parameters of underground parts and concentrations of phenylpropanoids, phenylethanoids, flavonoids, hydroxybenzoic acids, and catechins in aqueous–ethanol extracts of 6-year-old cultivated plants of Rhodiola rosea (propagated in vitro) of Altai Mountain origin were analyzed, and differences in chemical composition among plant specimens and between plant parts (rhizome and root) were evaluated. High-performance liquid chromatography detected 13 phenolic compounds. Roots contained 1.28 times higher phenylethanoids levels (1273.72 mg/100 g) than rhizomes did. Overall, the concentration of phenylethanoids in underground organs was not high and ranged from 21.36 to 103.00 mg/100 g. High variation among R. rosea individual plants was noted both in growth characteristics and in levels of secondary metabolites under our cultivation conditions. It was found that concentrations of phenylpropanoids, phenylethanoids, and catechins significantly depend on the plant part analyzed (p ≤ 0.05). Specimen No. 4 is characterized by the highest concentration of rosavins (1230.99 mg/plant) and the lowest concentration of cinnamyl alcohol (62.87 mg/plant). Despite the wide range of values, all 10 tested specimens (underground part) met the minimum requirements of the United States Pharmacopeia (2015) for rosavins (0.3%) and of the Russia State Pharmacopoeia (2015) for the average level of rosavins (roots): (1%)

Applying biotechnology in the propagation and further selection of vaccinium uliginosum × (V. corymbosum × V. angustifolium) hybrids

The most serious problem of intergeneric and interspecific hybridization is related to overcoming the reproductive isolation of different species. We assessed the efficiency of reproduction under in vitro conditions and the ex vitro growth capacity of interspecific hybrids of Vaccinium uliginosum (V. corymbosum V. angustifolium). The percentage of seed germination in in vitro culture was 88% for V. uliginosum, form No. 8 (V. corymbosum V. angustifolium), SC5-8, while it was 42% for V. uliginosum, form No. 8 (V. corymbosum V. angustifolium), ‘Northcountry’. The analysis of mean value showed that the multiplication rate increased and the shoot height decreased as the 2-isopentenyl adenine (2iP) concentration was increased in the nutrient medium of the studied hybrids. The maximum rate was achieved using 15 M 2iP. A detailed analysis of the hybrids indicated that the hybrid variant reliably affected growth and development indicators. Inter simple sequence repeat analysis demonstrated that all analyzed hybrids inherited DNA fragments of the parent plants in various combinations, confirming their hybrid nature. Thus, the use of in vitro methods for the propagation and further selection of genotypes is demonstrated as being an effective approach for developing interspecific hybrids of V. uliginosum (V. corymbosum V. angustifolium)

On the taxonomical status of Aquilegia kansuensis (Ranunculaceae)

Для рода Aquilegia L. (Ranunculaceae) предложена новая номенклатурная комбинация – A. kansuensis (Brühl) Erst.DOI: 10.14258/turczaninowia.17.4.4 A new combination in the genus Aquilegia L. (Ranunculaceae) – A. kansuensis (Brühl) Erst have been proposed.DOI: 10.14258/turczaninowia.17.4.

Investigation of chemical constituents of Eranthis longistipitata (Ranunculaceae): coumarins and furochromones

Aqueous‐ethanol extracts (70%) from the leaves of Eranthis longistipitata Regel. (Ranunculaceae Juss.)—collected from natural populations of Kyrgyzstan—were studied by liquid chromatography with high‐resolution mass spectrometry (LC‐HRMS). There was no variation of the metabolic profiles among plants that were collected from different populations. More than 160 compounds were found in the leaves, of which 72 were identified to the class level and 58 to the individual‐ compound level. The class of flavonoids proved to be the most widely represented (19 compounds), including six aglycones [quercetin, kaempferol, aromadendrin, 6‐methoxytaxifolin, phloretin, and (+)‐catechin] and mono‐ and diglycosides (the other 13 compounds). In the analyzed samples of E. longistipitata, 14 fatty acid–related compounds were identified, but coumarins and furochromones that were found in E. longistipitata were the most interesting result; furochromones khelloside, khellin, visnagin, and cimifugin were found in E. longistipitata for the first time. Coumarins 5,7‐dihydroxy‐4‐methylcoumarin, scoparone, fraxetin, and luvangetin and furochromones methoxsalen, 5‐O‐methylvisammioside, and visamminol‐3′‐O‐glucoside were detected for the first time in the genus Eranthis Salisb. For all the above compounds, the structural formulas are given. Furthermore, detailed information (with structural formulas) is provided on the diversity of chromones and furochromones in other representatives of Eranthis. The presence of chromones in plants of the genus Eranthis confirms its closeness to the genus Actaea L. because chromones are synthesized by normal physiological processes only in these members of the Ranunculaceae family

Trollius austrosibiricus (Ranunculaceae), a new species from South Siberia

Trolliusaustrosibiricus Erst & Luferov, sp. nov., a new species from Russian South Siberia is described and illustrated. This new species is endemic to Western and Central Siberia. Morphologically, it is close to the East Asian species T.chinensis and T.macropetalus. However, it differs from the aforementioned species due to the morphology of the rhizomes, aerial shoots, sepals and petals. This species is also distinguished from T.asiaticus, which is widespread in Russia (Western and Eastern Siberia), Mongolia, China, north-eastern Kazakhstan and in the northeast of the European part of Russia, in having a smaller number of sepals, longer persistent styles and petals longer than sepals. In addition, an identification key for all Russian species is given and all species have been discussed

Floristic findings in the Upper Yenisei Basin (2)

В результате экспедиционных исследований, анализа ранее опубликованных работ и просмотра материалов, хранящихся в гербариях ЦСБС (NS, NSK) и государственного природного заповедника «Азас», уточнены сведения о видовом составе флоры бассейна Верхнего Енисея. Впервые во флоре Тывы отмечены Humulus lupulus, Atriplex hortensis, Saponaria officinalis, Raphanus sativus, Abutilon theophrastii, Cichorium intybus, Elodea canadensis, Cypripedium × ventricosum, Poa sergievskajae; во флоре Хакасии – Microcerasus tomentosa, Cypripedium × ventricosum, S. viridis subsp. pycnocoma; во флоре южной части Красноярского края – Microcerasus tomentosa, Cypripedium × ventricosum. Уточнено распростране- ние и обнаружены новые местонахождения редких на этой территории видов – Asterothamnus heteropappoides, Ulmus pumila, Saxifraga nivalis, Euphorbia caesia.As a result of field work, analysis of the works published earlier and study of herbarium specimens in CSBG and State Nature Reserve «Azas», data on species composition of the Upper Yenisei Basin flora were verified. Humulus lupulus, Atriplex hortensis, Saponaria officinalis, Raphanus sativus, Abutilon theophrastii, Cichorium intybus, Elodea canadensis, Cypripedium × ventricosum and Poa sergievskajae were noted for the first time in the flora of Tyva, Microcerasus tomentosa, Cypripedium × ventricosum and Setaria viridis subsp. pycnocoma – in the flora of Khakasiya, Microcerasus tomentosa and Cypripedium × ventricosum – inthe flora of southern Krasnoyarsk Krai. Distribution was specified and new localities of rare species: Asterothamnus heteropappoides,Ulmus pumila, Saxifraga nivalis and Euphorbia caesia were found

Findings to the flora of Russia and adjacent countries: New national and regional vascular plant records, 4

With this paper we continue a new annual series, the main purpose of which is to make significant floristic findings from Russia and neighboring countries more visible in Russia and abroad. In total, this paper presents new records for 48 vascular plant species from 6 Eurasian countries, obtained during field explorations, as well as during taxonomic revisions of herbarium materials. For the first time, a new locality of Leontopodium leiolepis is recorded for Russia, Rheum uzengukuushi for China, Rorippa prolifera for Lithuania, Lappula marginata for Kyrgyzstan and Tajikistan, Anthriscus caucalis, Chenopodium ficifolium, Euphorbia prostrata for Uzbekistan, Adonis × hybrida, Potamogeton × franconicus, Solidago × niederederi for the Asian part of Russia, Echinochloa esculenta, Poa jamalinensis, Puccinellia poecilantha for Siberia, Potentilla intermedia for the Caucasus, Rhynchospora alba for the Russian part of Altai, Poa sphondylodes, Veronica beccabunga for Eastern Siberia, Asclepias syriaca for the Republic of Altai, Chimaphila umbellata, Orobanche korshinskyi, Veronica scutellata for the Republic of Buryatia, Cirsium alatum, Thalictrum simplex for the Republic of Crimea, Thymus rariflorus, Th. terekensis for the Republic of Ingushetia, Berberis thunbergii, Crataegus maximowiczii, Prunus serotina for the Republic of Mordovia, Oenothera villosa for the Republic of Tatarstan, Astragalus sulcatus, Galium mollugo for the Republic of Tyva, Phragmites altissimus for the Chelyabinsk Region, Senecio dubitabilis for the Magadan Region, Asclepias syriaca, Galatella villosa, Potentilla recta for the Novosibirsk Region, Dodartia orientalis for the Omsk Region, Viola hultenii for the Sakhalin Region, Phragmites tzvelevii for the Samara Region and the Middle Volga, Jacobaea ferganensis for the Samara Region, Carex media, Impatiens parviflora for the Tyumen Region. There are some more findings which are not new for the region but they contribute significantly to the understanding of species distribution

Optimization of Biomass Accumulation and Production of Phenolic Compounds in Callus Cultures of Rhodiola rosea L. Using Design of Experiments

Rhodiola rosea L. is a valuable medicinal plant with adaptogenic, neuroprotective, antitumor, cardioprotective, and antidepressant effects. In this study, design of experiments methodology was employed to analyze and optimize the interacting effects of mineral compounds (concentration of NO3− and the ratio of NH4+ to K+) and two plant growth regulators [total 6-benzylaminopurine (BAP) and α-naphthylacetic acid (NAA) concentration and the ratio of BAP to NAA] on the growth and the production of total phenolic compounds (TPCs) in R. rosea calluses. The overall effect of the model was highly significant (p < 0.0001), indicating that NH4+, K+, NO3−, BAP, and NAA significantly affected growth. The best callus growth (703%) and the highest production of TPCs (75.17 mg/g) were achieved at an NH4+/K+ ratio of 0.33 and BAP/NAA of 0.33, provided that the concentration of plant growth regulators was 30 μM and that of NO3− was ≤40 mM. According to high-performance liquid chromatography analyses of aerial parts (leaves and stems), in vitro seedlings and callus cultures of R. rosea contain no detectable rosarin, rosavin, rosin, and cinnamyl alcohol. This is the first report on the creation of an experiment for the significant improvement of biomass accumulation and TPC production in callus cultures of R. rosea

Effect of concentration, method of auxin application and cultivation conditions on in vitro rooting of bog blueberry (Vaccinium uliginosum L.)

BACKGROUND:Bog blueberry, Vaccinium uliginosum is one of the newer fruit crops. Choosing the most appropriate methods for mass vegetative propagation of V. uliginosum cultivars, including in vitro propagation, is crucial. OBJECTIVE:We investigated the effect of growth regulators and light on in vitro rhizogenesis of V. uliginosum to determine the optimal method of propagation. METHODS:The following cultivation techniques were tested on microshoots of bog blueberry varieties ‘Golubaya rossyp’, ‘Nektarnaya’, ‘Shegarskaya’ and ‘Iksinskaya’: 1) ½ Anderson (A) medium supplemented with 3 and 10 μM indole-3-butyric acid (IBA) or indole-3-acetic acid (IAA) and 2) 24-h aqueous treatments of microshoots with 148 μM IBA or 171 μM IAA and further cultivation in ½ A medium. In addition, we tested precultivation in darkness for 3 d versus a 16/8-h photoperiod. RESULTS:100% rooting was observed in ‘Golubaya rossyp’ in all treatments of the experiments and with ‘Nektarnaya’ on the growth-regulator-free ½ A medium and after treating the microshoots with 171 μM IAA solution. Precultivation in darkness produced a positive effect on rooting in ‘Shegarskaya’ and ‘Iksinskaya.’ CONCLUSIONS:Response to in vitro rooting techniques varied between cultivars of V. uliginosum. Treatment of microshoots with auxin solutions improved in vitro rooting

Effect of concentration, method of auxin application and cultivation conditions on in vitro rooting of bog blueberry (Vaccinium uliginosum L.)

BACKGROUND:Bog blueberry, Vaccinium uliginosum is one of the newer fruit crops. Choosing the most appropriate methods for mass vegetative propagation of V. uliginosum cultivars, including in vitro propagation, is crucial. OBJECTIVE:We investigated the effect of growth regulators and light on in vitro rhizogenesis of V. uliginosum to determine the optimal method of propagation. METHODS:The following cultivation techniques were tested on microshoots of bog blueberry varieties ‘Golubaya rossyp’, ‘Nektarnaya’, ‘Shegarskaya’ and ‘Iksinskaya’: 1) ½ Anderson (A) medium supplemented with 3 and 10 μM indole-3-butyric acid (IBA) or indole-3-acetic acid (IAA) and 2) 24-h aqueous treatments of microshoots with 148 μM IBA or 171 μM IAA and further cultivation in ½ A medium. In addition, we tested precultivation in darkness for 3 d versus a 16/8-h photoperiod. RESULTS:100% rooting was observed in ‘Golubaya rossyp’ in all treatments of the experiments and with ‘Nektarnaya’ on the growth-regulator-free ½ A medium and after treating the microshoots with 171 μM IAA solution. Precultivation in darkness produced a positive effect on rooting in ‘Shegarskaya’ and ‘Iksinskaya.’ CONCLUSIONS:Response to in vitro rooting techniques varied between cultivars of V. uliginosum. Treatment of microshoots with auxin solutions improved in vitro rooting