4,120 research outputs found

    Effect of Piper betle and Brucea javanica on the Differential Expression of Hyphal Wall Protein (HWP1) in Non-Candida albicans Candida (NCAC) Species

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    The study aimed to identify the HWP1 gene in non-Candida albicans Candida species and the differential expression of HWP1 following treatment with Piper betle and Brucea javanica aqueous extracts. All candidal suspensions were standardized to 1 x 10(6) cells/mL. The suspension was incubated overnight at 37 degrees C (C. parapsilosis, 35 degrees C). Candidal cells were treated with each respective extract at 1, 3, and 6 mg/mL for 24 h. The total RNA was extracted and reverse transcription-polymerase chain reaction was carried out with a specific primer of HWP1. HWP1 mRNAs were only detected in C. albicans, C. parapsilosis, and C. tropicalis. Exposing the cells to the aqueous extracts has affected the expression of HWP1 transcripts. C. albicans, C. parapsilosis, and C. tropicalis have demonstrated different intensity of mRNA. Compared to P. betle, B. javanica demonstrated a higher suppression on the transcript levels of HWP1 in all samples. HWP1 was not detected in C. albicans following the treatment of B. javanica at 1 mg/mL. In contrast, C. parapsilosis and C. tropicalis were shown to have HWP1 regulation. However, the expression levels were reduced upon the addition of higher concentration of B. javanica extract. P. betle and B. javanica have potential to be developed as oral health product.Article Link : http://www.hindawi.com/journals/ecam/2013/397268

    Reach the person behind the dementia: physical therapists' reflections and strategies when composing physical training

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    Dementia is a disease characterized by cognitive impairment and physical decline that worsens over time. Exercise is one lifestyle factor that has been identified as a potential means of reducing or delaying progression of the symptoms of dementia, maximizing function and independence. The purpose of this study was to explore physical therapists' (PTs) experiences and reflections on facilitating high-intensity functional exercise with older people living with dementia, in residential care home settings. The study used a qualitative design based on interviews, individually or in small groups, with seven PTs engaged as leaders in the training of older people with dementia. The interviews were analyzed with a modified Grounded Theory method with focus on constant comparisons. To increase trustworthiness the study used triangulation within investigators and member checking. The core category "Discover and act in the moment-learn over time" reflects how the PTs continuously developed their own learning in an iterative process. They built on previous knowledge to communicate with residents and staff and to tailor the high intensity training in relation to each individual at that time point. The category "Be on your toes" highlights how the PTs searched for sufficient information about each individual, before and during training, by eliciting the person's current status from staff and by interpreting the person's body language. The category "Build a bond with a palette of strategies" describes the importance of confirmation to build up trust and the use of group members and the room to create an interplay between exercise and social interaction. These findings highlight the continuous iterative process of building on existing knowledge, sharing and reflecting, being alert to any alterations needed for individuals that day, communication skills (both with residents and staff) and building a relationship and trust with residents in the effective delivery of high intensity functional exercise to older people living with dementia in care settings

    Future Community-Based Ecotourism (CBET) development

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    Ecotourism is an alternative form of tourism and is usually confused with natural and cultural tourism.CBET is fast becoming a popular biodiversity conservation tool that develops and benefits the local community. Based on the context of conservation theory and practice, Community-Based Ecotourism (CBET) is a form of community-based natural resource management. However, a sustainable CBET development through Community Capacity Building (CCB)programs is not something that it easily achievable. Local community’s capacity varies from one culture to another. It takes a high level of community participation, in order for it to come to a level where the community members themselves are motivated to participate and contribute to the development of the program. This fully qualitative research involved 15 respondents from the community of Kg. Selai, Bekok in Johor, Malaysia. The result show there are five factors that sustained the ecotourism development based for Orang Asli Community in Kg. Selai, namely, existing CBET development, past CBET development, local community participation in planning stage of tourism, local participation in implementation stage of tourism and participation in nature conservation

    Simulation Studies of Gas-Solid in the Riser of a Circulating Fluidized Bed

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    A numerical parametric study was performed on the influence of various riser exit geometries on the hydrodynamics of gas-solid two-phase flow in the riser of a Circulating Fluidized Bed (CFB). A Eulerian continuum formulation was applied to both phases. A two fluid framework has been used to simulate fully developed gas-solid flows in vertical riser. A two dimensional Computational Fluid Dynamics (CFD) model of gas-particle flow in the CFB has been investigated using the code FLUENT. The turbulence was modeled by a k-e turbulence model in the gas phase. The simulations were done using the geometrical configuration of a CFB test rig at the Universiti Teknologi Malaysia (UTM). The CFB riser column has 265 mm (width), 72 mm (depth) and 2.7 m height. The riser is made up of interchangeable Plexiglas columns. The computational model was used to simulate the riser over a wide range of operating and design parameters. In addition, several numerical experiments were carried out to understand the influence of riser end effects, particle size, gas solid velocity and solid volume fraction on the simulated flow characteristics. The CFD model with a k-e turbulence model for the gas phase and a fixed particle viscosity in the solids phase showed good mixing behaviour. These results were found to be useful in further development of modeling of gas solid flow in the riser

    Normal soaking extraction (NSE) of rotenone from Derris elliptica

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    Derris elliptica or the tuba plant contains rotenone, bio-active compound known that has the potential to be used as bio-pesticide. Bio-pesticide is the best-known alternative bio-pesticide which has the potential to replace the use of conventional pesticides, as it is more environmentally friendly. The main objective of the paper is to obtain the best extraction solvent for optimizing the yield in rotenone extraction. Rotenone was extracted from Derris root using three different parts of roots and three types of solvent in Normal Soaking Extraction (NSE). The types of Derris roots are: (1) Fine root (2) Coarse root (3) Stem. The three types of solvent system are: (1) Acetone 95 % (v/v), (2) Chloroform 99.9 % (v/v) and (3) a mixture of ethanol: H2O (9:1) added with oxalic acid (1mg/ml). The liquid crude extracts were further cleaned up to remove the fine debris of roots. The presence of rotenone was confirmed using qualitative analysis Thin Layer Chromatography (TLC) and thereupon the determination of rotenone content was carried out using High Performance Liquid Chromatography (HPLC). From the results obtained, it was found that the Normal Soaking Extraction (NSE) using acetone 95 % (v/v) was the best method to extract the highest yield of rotenone; 1.14 % (w/w)

    Amalan pengurusan keselamatan bengkel kemahiran hidup (KHB) Sekolah Menengah Harian Daerah Batu Pahat

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    Kajian ini dijalankan untuk mengkaji sejauhmanakah persepsi pelajar terhadap amalan pengurusan keselamatan dari aspek keselamatan alatan dan bahan, diri dan rakan serta keselamatan am di bengkel Kemahiran Hidup sekolah menengah harian daerah Batu Pahat. Kajian berbentuk kuantitatif dilaksanakan di empat buah sekolah menengah harian daerah Batu Pahat iaitu SMK Datin Onn Jaafar, SMK Banang Jaya, SMK Dato Onn dan SMK Seri Gading. Sampel kajian terdiri daripada 350 orang pelajar tingkatan dua yang mengambil matapelajaran KHB dan data dikumpul menggunakan instrumen borang soal selidik. Dapatan kajian dianalisis menggunakan perisian Winsteps Version 3.69.1.11 dengan pendekatan Model Pengukuran Rasch sepenuhnya. Data dianalisis menggunakan teknik analisis diskriptif dan analisis inferensi iaitu ujian-t bagi menguji hipotesis kajian. Dapatan kajian menunjukkan bahawa amalan pengurusan keselamatan di bengkel diamalkan secara sederhana bagi ketiga-tiga aspek tersebut. Terdapat perbezaan yang signifikan di antara pelajar di bandar dan luar bandar iaitu t=-4.08 dan p=0.000 di mana nilai min ukuranya pula ialah -.19. Beberapa cadangan telah dikemukakan bagi penambahbaikkan seperti mengadakan kempen kesedaran dan menyediakan sudut standard operasi amalan pengurusan keselamatan agar pelajar sentiasa mematuhi peraturan semasa berada di bengkel Kemahiran Hidup

    Near Shore Environment and Its Corrosiveness Towards Steel Pipelines

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    Seabed sediment is an important environment that has significant effect on pipelines buried under it. Hereby, study on corrosion rate of actual steel pipelines has been conducted to highlight the importance of near shore environment as an aggressive environment towards steel structures. Coupons made of X70 steel pipelines have been considered for weight lost determination in order to calculate the corrosion rate at both near shore and offshore areas. The results show that the corrosion rate of coupons buried at near shore area was higher than the coupons at offshore. Conclusively near shore environment has shown a significant influence on corrosion of steel pipelines

    Slope Mass Rating (SMR) Classification for Rock Slope Stability and Geohazard Vulnerability Assessment

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    Quantitative classification from the Slope Mass Rating (SMR) has introduced an empirical assessment for rock slope stability rating. This stability classification enables us to foresee the magnitude and susceptibility of rock mass failure towards adjacent infrastructure and human life. In this study, the stability of quarry rock slope was classified using SMR classification system and kinematic stability analysis before being correlated with geohazard vulnerability assessment. Geological formation study with site geographical survey from aerial photogrammetry technique provided comprehensive data for the study area. Fieldwork to evaluate the SMR assessment parameters was conducted by discontinuity mapping using scanline method. In-situ evaluation of rock mass such as rebound surface hardness and discontinuity characterization was carried out with rock material sampling for laboratory testing. Kinematic stability analysis presented an estimation for the direction and mode of rock slope failure. The SMR classification that constitutes geomechanics attributes had introduced the global stability rating of rock mass structure, thus facilitating the prediction of potential failure magnitude. Hence, the integration of these stability indications provides sufficient empirical estimation for geohazard vulnerability zoning for the studied area
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