A Family of DNA Sequences is Reproducibly Rearranged in the Somatic Nucleus of \u3cem\u3eTetrahymena\u3c/em\u3e

A small family of DNA sequences Is rearranged during the development of the somatic nucleus in Tetrahymena. The family is defined by 266 bp of highly conserved sequence which restriction mapping, hybridization and sequence analysis have shown is shared by a cloned micronuclear fragment and three sequences which constitute the macronuclear family. Genomic Southern hybridization experiments indicate there are five members of the family in micronuclear DNA. All of the family members are present in whole genome homozygotes and are therefore nonallellic. The three macronuclear sequences are all present in clonal cell lines and are reproducibly generated in every developing macronucleus. The rearrangement event begins 14 hours after conjugation is initiated and is nearly completed by 16 hours

Society and the Balance of Professional Dominance, and Patient Autonomy in Medical Care

Symposium: Emerging Paradigms in Bioethic

Working with children suffering from abuse and neglect : the elementary school counselor as an advocate

This paper examines the history of advocacy for the rights and safety of children suffering from abuse and neglect in the United States, and more specifically the state of Iowa. It looks at statistical data concerning the incidence of child abuse and its correlation to age and ability level. It specifically addresses the developmental, psychological, emotional, and physical effects of child abuse. The remainder of the paper outlines the role of the elementary school counselor as an advocate in helping children who are suffering from abuse and neglect. The paper addresses individual, group, and classroom interventions that may be used in assisting this growing population

Unleashing Corporate Entrepreneurship

Noncompetition agreements (noncompetes), which prohibit employees from launching or working at competitive companies for certain periods, have become increasingly prevalent in the workplace. Employers claim they need noncompetes to protect their trade secrets and other legitimate business interests, but most workers do not have access to trade secrets—and when they do, such secrets can be better protected through confidentiality and intellectual property agreements. In practice, many companies appear to use noncompetes as an employee retention tool, but this is not a legitimate purpose for a noncompete. In addition, noncompetes have a disproportionately negative impact on women, people of color, and low-wage workers and are challenging to impose on the modern, remote workforce. Negative media attention concerning the proliferation and abuse of noncompetes has recently led numerous states to pass legislation limiting their use. In addition, the Federal Trade Commission recently issued a noncompete ban that, if upheld, would create a sweeping change in workplace law, but it is subject to judicial challenge. However, in the flurry of activity, policymakers have not sufficiently considered the employee retention motive underlying many employers’ noncompetes. Noncompetes are built on a corporate culture where employee exit is presumed—and feared. This article suggests a different approach: creating a culture that decreases employees’ incentives to exit while increasing their incentives to remain, thereby reducing the need for noncompetes. The article makes two main claims. First, it argues that the government should ban noncompetes, other than for highly compensated employees, because noncompetes are increasingly unenforceable, have adverse effects, and are often used for illegitimate reasons. Second, it argues that companies should encourage intrapreneurship—i.e., entrepreneurship within a company—rather than noncompetes, which discourage entrepreneurship. This counterintuitive approach is grounded in organizational behavior and sociological theories about the modern workplace, in which millennials are now the largest generation. Intrapreneurship fosters innovation and employee retention in a more effective and less harmful manner than noncompetes, which have become incongruent with the modern workforce

Social Network Dynamics and Biographical Disruption: The Case of “First-Timers” with Mental Illness

This study examines how dynamics surrounding biographical disruptions compare to more routine fluctuations in personal social networks. Using data from the Indianapolis Network Mental Health Study, the authors track changes in patients’ social networks over three years and compare them to a representative sample of persons with no self-reported mental illness. Overall, individuals at the onset of treatment report larger and more broadly functional social networks than individuals in the population at large. However, the number of network ties among the latter increases over time, whereas network size decreases slightly among people using mental health services. As individuals progress through treatment, less broadly supportive ties drop out of extended networks, but a core safety net remains relatively intact. The findings in this case provide evidence that social network dynamics reflect changing needs and resources: persons labeled with psychiatric disorders learn to manage illness, with functionality driving social interaction in times of biographical disruption

Platelet-mediated metabolism of the common dietary flavonoid, quercetin.

BACKGROUND: Flavonoid metabolites remain in blood for periods of time potentially long enough to allow interactions with cellular components of this tissue. It is well-established that flavonoids are metabolised within the intestine and liver into methylated, sulphated and glucuronidated counterparts, which inhibit platelet function. METHODOLOGY/PRINCIPAL FINDINGS: We demonstrate evidence suggesting platelets which contain metabolic enzymes, as an alternative location for flavonoid metabolism. Quercetin and a plasma metabolite of this compound, 4'-O-methyl quercetin (tamarixetin) were shown to gain access to the cytosolic compartment of platelets, using confocal microscopy. High performance liquid chromatography (HPLC) and mass spectrometry (MS) showed that quercetin was transformed into a compound with a mass identical to tamarixetin, suggesting that the flavonoid was methylated by catechol-O-methyl transferase (COMT) within platelets. CONCLUSIONS/SIGNIFICANCE: Platelets potentially mediate a third phase of flavonoid metabolism, which may impact on the regulation of the function of these cells by metabolites of these dietary compounds

Emotional Intelligence – Women Entrepreneurs' Secret Weapon – A Conceptual Study

In the contemporary context, the increasing number of Women Entrepreneurs across the globe has approved the myth that emotional intelligence is integrated only with men. Women are wired with an innate sense of emotional intelligence which capacitate equally successful with men. Women are more triumphant as entrepreneurs as they effectively manage their work and family responsibilities. The calibre encompassed with emotional intelligence creates effective women entrepreneurs enabling them to build strong interpersonal skills, provide confidence substantially and increase resilience to tautness. These qualities have been the secret weapons for women’s entrance and success as entrepreneurs entering into the business world. Often women entrepreneurs sway between challenges, risks and difficulties, in such occurrence prudent utilization of emotions resulted in sound decision making. It is evident that emotionally intelligent women can ascertain and comprehend the emotions of both themselves and others, which steer the workers towards the goal. This analysis provides important insight into the impact of emotional intelligence on women entrepreneurs for those concerned about their effectiveness which leads to organization growth at large. It is true that the skills of emotional intelligence are the important factors in complex market place which help the female entrepreneurs to build workplaces where people thrive. This paper attempts to analyze the beneficial effect of emotional intelligence on stress management cognitive stability of women who sustain the challenges in the industrial world. Keywords: Emotional Intelligence, Women Entrepreneur, Secret Weapo

Toward a More Perfect Union: Overcoming Division, Discrimination, and Distance Through the Rule of Law

This Essay is based on remarks delivered on February 15, 2023, at the Distinguished Jurist in Residence Lecture held at Fordham University School of Law and hosted by the Center for Judicial Events and Clerkships.https://ir.lawnet.fordham.edu/jirl/1004/thumbnail.jp

The relationship of certain word analysis abilities to the reading achievement of first, second, and third grade children,

Thesis (M.A.)--Boston Universit

The secretome of alginate-encapsulated limbal epithelial stem cells modulates corneal epithelial cell proliferation

Limbal epithelial stem cells may ameliorate limbal stem cell deficiency through secretion of therapeutic proteins, delivered to the cornea in a controlled manner using hydrogels. In the present study the secretome of alginate-encapsulated limbal epithelial stem cells is investigated. Conditioned medium was generated from limbal epithelial stem cells encapsulated in 1.2% (w/v) calcium alginate gels. Conditioned medium proteins separated by 1-D gel electrophoresis were visualized by silver staining. Proteins of interest including secreted protein acidic and rich in cysteine, profilin-1, and galectin-1 were identified by immunoblotting. The effect of conditioned medium (from alginate-encapsulated limbal epithelial stem cells) on corneal epithelial cell proliferation was quantified and shown to significantly inhibit (P</=0.05) their growth. As secreted protein acidic and rich in cysteine was previously reported to attenuate proliferation of epithelial cells, this protein may be responsible, at least in part, for inhibition of corneal epithelial cell proliferation. We conclude that limbal epithelial stem cells encapsulated in alginate gels may regulate corneal epithelialisation through secretion of inhibitory proteins