Development of an Adventist Community Services Center To Serve Navajo Residents of Kayenta, Arizona

The Problem A decision had been made to establish a Seventh-day Adventist Community Services Center in Kayenta, Arizona, on the Navajo Indian Reservation, in connection with a church-planting initiative. Those in charge lacked experience and training in community services ministries and faced obstacles to implementation of the plan. The Method A combination of research and action was used to move the envisioned Community Services Center toward reality. Study of the Bible and related literature led to formulation of Scripture-based methodological principles. Demographic research and community needs assessment procedures were utilized to gain an understanding of the Kayenta community and especially of its social needs. In cooperation with the local Native American Seventh- day Adventist congregations and also with regional church organizations and officials, efforts were made to obtain land, erect a building, select personnel, and implement community service ministries in Kayenta. The Results Land was obtained and a Community Services Center building was erected in Kayenta. Community needs were ascertained and potential Adventist service projects were enumerated. A full-time Center director was employed and a budget for the first year of operation was developed and partially implemented. Methodological and theological uncertainties were clarified. External factors, including disagreements among the participating organizations regarding methodological and theological principles, hindered full operational implementation of the envisioned Center. At the project’s conclusion, Adventists were not yet providing substantial community service ministries in Kayenta. Conclusions For Adventist Community Services ministries to Native Americans to be effective in the context of God’s revealed purposes for the well-being of humankind, they must be conducted wholistically and in close connection with the church body. Service providers must deeply understand the needs, problems, and life situation of the people served. Spiritually based criteria need to be used when selecting project personnel, and the local Native church should be involved in planning, supporting, and operating the Center. Finances for Native American Christian humanitarian projects are not an insurmountable obstacle, with a variety of funding sources available

Fundamental Aspects of Wood Deformation Pertaining To Manufacture of Wood-Based Composites

During processing, wood-based composites are pressed using extreme heat and pressure for varying lengths of time. Evidence exists that the environmental conditions under which the wood densifies can alter the properties of both the solid wood and the composite product. Given the larger number and extreme nature of variables that exist during composite manufacture, it is imperative that the deformation process be understood from a fundamental standpoint. The objective of this research was to determine the applicability of basic materials engineering theory to the viscoelastic deformation of wood in transverse compression under a variety of temperatures and moisture contents.Theories of cellular solids were used to model the nonlinear compression behavior of small wood elements. For low-density woods, it was determined that cellular collapse can result from elastic buckling of the cell wall. The dependence of inelastic behavior of the gross wood on the elastic properties of the cell wall allows the time, temperature, and moisture dependence to be modeled with classical linear viscoelastic theory of amorphous polymers. Time-temperature-moisture superposition was shown to be applicable to stress relaxation data collected for temperatures between 39 and 99 C and moisture contents between 3 and 16%. The shift factors derived were described using free volume and entropy-based equations. This research demonstrates that wood behaves similarly under those conditions to the general class of cellular amorphous polymers. This conclusion opens many possibilities for experimentally and mathematically modeling the pressing of wood-based composites

Fundamentals of Flakeboard Manufacture: Viscoelastic Behavior of the Wood Component

Theories of the viscoelastic behavior of amorphous polymers are reviewed and are used to describe the density gradient formation in flakeboard. This technique utilizes measured temperature and gas pressure at discrete locations inside a flake mat during hot pressing to predict the glass transition temperature of wood as a function of press time. The difference between the flake temperature and the predicted glass transition temperature is a relative indicator of the amount of flake deformation and stress relaxation at a location in the mat. A knowledge of the stress history imposed in the mat is then used to relate flake deformation and stress relaxation to the formation of a density gradient. This analysis allows for a significant portion of the density gradient to develop after the hot press has closed. Experimental data for various density gradients support the theories presented here

Sustainability of a long-term volunteer-based bird monitoring program : recruitment, retention and attrition

With an increasing demand and reliance on volunteers in a host of different sectors, the need for organisations to understand motivating factors behind volunteering, and how to retain volunteer services, is crucial. This paper examines the recruitment, retention and attrition among a group of volunteers participating in a long-term monitoring program for the critically endangered orange-bellied parrot Neophema chrysogaster. This project is one of the longest running of its type in Australia with more than 20 years of survey activities. Volunteers were most often recruited via other environmental agencies. Almost three-quarters of respondents had been involved in birdwatching or other bird monitoring activities usually in connection with conservation organisations. The modal response was an involvement of 10-20 years, with most other responses in the range 0-5 years. The majority intend to continue volunteering, but cited perceived health and time commitments as the main factors behind possible future decisions to cease volunteering. Respondents suggested improvements in the volunteer management of the program, and in particular, thought a personal \u27thank you\u27, would maintain motivation and participation

Time-resolved energy transfer from single chloride terminated nanocrystals to graphene

We examine the time-resolved resonance energy transfer of excitons from single n-butyl amine-bound, chloride-terminated nanocrystals to two-dimensional graphene through time-correlated single photon counting. The radiative biexponential lifetime kinetics and blinking statistics of the individual surface-modified nanocrystal elucidate the non-radiative decay channels. Blinking modification as well as a 4 times reduction in spontaneous emission were observed with the short chloride and n-butylamine ligands, probing the energy transfer pathways for the development of graphene-nanocrystal nanophotonic devices

Survey of bacterial diversity in chronic wounds using Pyrosequencing, DGGE, and full ribosome shotgun sequencing

<p>Abstract</p> <p>Background</p> <p>Chronic wound pathogenic biofilms are host-pathogen environments that colonize and exist as a cohabitation of many bacterial species. These bacterial populations cooperate to promote their own survival and the chronic nature of the infection. Few studies have performed extensive surveys of the bacterial populations that occur within different types of chronic wound biofilms. The use of 3 separate16S-based molecular amplifications followed by pyrosequencing, shotgun Sanger sequencing, and denaturing gradient gel electrophoresis were utilized to survey the major populations of bacteria that occur in the pathogenic biofilms of three types of chronic wound types: diabetic foot ulcers (D), venous leg ulcers (V), and pressure ulcers (P).</p> <p>Results</p> <p>There are specific major populations of bacteria that were evident in the biofilms of all chronic wound types, including <it>Staphylococcus, Pseudomonas, Peptoniphilus, Enterobacter, Stenotrophomonas, Finegoldia</it>, and <it>Serratia </it>spp. Each of the wound types reveals marked differences in bacterial populations, such as pressure ulcers in which 62% of the populations were identified as obligate anaerobes. There were also populations of bacteria that were identified but not recognized as wound pathogens, such as <it>Abiotrophia para-adiacens </it>and <it>Rhodopseudomonas </it>spp. Results of molecular analyses were also compared to those obtained using traditional culture-based diagnostics. Only in one wound type did culture methods correctly identify the primary bacterial population indicating the need for improved diagnostic methods.</p> <p>Conclusion</p> <p>If clinicians can gain a better understanding of the wound's microbiota, it will give them a greater understanding of the wound's ecology and will allow them to better manage healing of the wound improving the prognosis of patients. This research highlights the necessity to begin evaluating, studying, and treating chronic wound pathogenic biofilms as multi-species entities in order to improve the outcomes of patients. This survey will also foster the pioneering and development of new molecular diagnostic tools, which can be used to identify the community compositions of chronic wound pathogenic biofilms and other medical biofilm infections.</p

Innovative mechanism for measuring the mass properties of an object

The Kennedy Space Center Robotics Group recently completed development and testing on a novel approach to measure the mass properties of a rigid body. This unique design can measure the payload's weight, mass center location, and moments of inertia about three orthogonal axes. Furthermore, these measurements only require a single torque sensor and a single angular position sensor

Multi-platform comparison of ten commercial master mixes for probe-based real-time polymerase chain reaction detection of bioterrorism threat agents for surge preparedness

The Centers for Disease Control and Prevention and United States Army Research Institute for Infectious Diseases have developed real-time PCR assays for the detection of bioterrorism threat agents. These assays all rely on a limited number of approved real-time PCR master mixes. Because the availability of these reagents is a critical element of bioterrorism preparedness, we undertook a joint national preparedness exercise to address the potential surge needs resulting from a large-scale bio-emergency. We identified 9 commercially-available potential alternatives to an existing approved master mix (LightCycler FastStart DNA Master HybProbes): the TaqMan Fast Universal PCR master mix, OmniMix HS, FAST qPCR master mix, EXPRESS qPCR SuperMix kit, QuantiFast Probe PCR kit, LightCycler FastStart DNA MasterPLUS HybProbe, Brilliant II FAST qPCR master mix, ABsolute Fast QPCR Mix and the HotStart IT Taq master mix. The performances of these kits were evaluated by the use of real-time PCR assays for four bioterrorism threat agents: Bacillus anthracis, Brucella melitensis, Burkholderia mallei and Francisella tularensis. The master mixes were compared for target-specific detection levels, as well as consistency of results among three different real-time PCR platforms (LightCycler, SmartCycler and 7500 Fast Dx). Realtime PCR analysis revealed that all ten kits performed well for agent detection on the 7500 Fast Dx instrument; however, the QuantiFast Probe PCR kit yielded the most consistently positive results across multiple real-time PCR platforms. We report that certain combinations of commonly used master mixes and instruments are not as reliable as others at detecting low concentrations of target DNA. Furthermore, our study provides laboratories the option to select from the commercial kits we evaluated to suit their preparedness needs

Multi-platform comparison of ten commercial master mixes for probe-based real-time polymerase chain reaction detection of bioterrorism threat agents for surge preparedness

The Centers for Disease Control and Prevention and United States Army Research Institute for Infectious Diseases have developed real-time PCR assays for the detection of bioterrorism threat agents. These assays all rely on a limited number of approved real-time PCR master mixes. Because the availability of these reagents is a critical element of bioterrorism preparedness, we undertook a joint national preparedness exercise to address the potential surge needs resulting from a large-scale bio-emergency. We identified 9 commercially-available potential alternatives to an existing approved master mix (LightCycler FastStart DNA Master HybProbes): the TaqMan Fast Universal PCR master mix, OmniMix HS, FAST qPCR master mix, EXPRESS qPCR SuperMix kit, QuantiFast Probe PCR kit, LightCycler FastStart DNA MasterPLUS HybProbe, Brilliant II FAST qPCR master mix, ABsolute Fast QPCR Mix and the HotStart IT Taq master mix. The performances of these kits were evaluated by the use of real-time PCR assays for four bioterrorism threat agents: Bacillus anthracis, Brucella melitensis, Burkholderia mallei and Francisella tularensis. The master mixes were compared for target-specific detection levels, as well as consistency of results among three different real-time PCR platforms (LightCycler, SmartCycler and 7500 Fast Dx). Realtime PCR analysis revealed that all ten kits performed well for agent detection on the 7500 Fast Dx instrument; however, the QuantiFast Probe PCR kit yielded the most consistently positive results across multiple real-time PCR platforms. We report that certain combinations of commonly used master mixes and instruments are not as reliable as others at detecting low concentrations of target DNA. Furthermore, our study provides laboratories the option to select from the commercial kits we evaluated to suit their preparedness needs

Ultrasonic Plate Wave Evaluation of Natural Fiber Composite Panels

Two key shortcomings of current ultrasonic nondestructive evaluation (NDE) techniques for plywood, medium density fiberboard (MDF), and oriented strandboard are the reliance on empirical correlations and the neglect of valuable waveform information. The research reported herein examined the feasibility of using fundamental mechanics, wave propagation, and laminated, shear deformable plate theories to nondestructively evaluate material properties in natural fiber-based composite panels. Dispersion curves were constructed exhibiting the variation of flexural plate wave phase velocity with frequency. Based on shear deformable laminated plate wave theory, flexural and transverse shear rigidity values for solid transversely isotropic, laminated transversely isotropic, and solid orthotropic natural fiber-based composite panels were obtained from the dispersion curves. Axial rigidity values were obtained directly from extensional plate wave phase velocity. Excellent agreement (within 3%) of flexural rigidity values was obtained between NDE and mechanical testing for most panels. Transverse shear modulus values obtained from plate wave tests were within 4% of values obtained from through-thickness ultrasonic shear wave speed. Tensile and compressive axial rigidity values obtained from NDE were 22% to 41% higher than mechanical tension and compression test results. These differences between NDE and axial mechanical testing results are likely due to load-rate effects; however, these large differences were not apparent in the flexural and transverse shear comparisons. This fundamental research advances the state-of-the-art of NDE of wood-based composites by replacing empirical approaches with a technique based on fundamental mechanics, shear deformation laminated plate theory, and plate wave propagation theory