Substrate-Specific Inhibition Constants for Phospholipase A2 Acting on Unique Phospholipid Substrates in Mixed Micelles and Membranes Using Lipidomics.

Assaying lipolytic enzymes is extremely challenging because they act on water-insoluble lipid substrates, which are normally components of micelles, vesicles, and cellular membranes. We extended a new lipidomics-based liquid chromatographic-mass spectrometric assay for phospholipases A2 to perform inhibition analysis using a variety of commercially available synthetic and natural phospholipids as substrates. Potent and selective inhibitors of three recombinant human enzymes, including cytosolic, calcium-independent, and secreted phospholipases A2 were used to establish and validate this assay. This is a novel use of dose-response curves with a mixture of phospholipid substrates, not previously feasible using traditional radioactive assays. The new application of lipidomics to developing assays for lipolytic enzymes revolutionizes in vitro testing for the discovery of potent and selective inhibitors using mixtures of membranelike substrates

System for Configuring Modular Telemetry Transponders

A system for configuring telemetry transponder cards uses a database of error checking protocol data structures, each containing data to implement at least one CCSDS protocol algorithm. Using a user interface, a user selects at least one telemetry specific error checking protocol from the database. A compiler configures an FPGA with the data from the data structures to implement the error checking protocol

Adaptable Transponder for Multiple Telemetry Systems

The present invention is a stackable telemetry circuit board for use in telemetry systems for satellites and other purposes. The present invention incorporates previously-qualified interchangeable circuit boards, or "decks," that perform functions such as power, signal receiving and transmission, and processing. Each deck is adapted to serve a range of telemetry applications. This provides flexibility in the construction of the stackable telemetry circuit board and significantly reduces the cost and time necessary to develop a telemetry system

Initial Results from On-Orbit Testing of the Fram Memory Test Experiment on the Fastsat Micro-Satellite

The Memory Test Experiment is a space test of a ferroelectric memory device on a low Earth orbit satellite that launched in November 2010. The memory device being tested is a commercial Ramtron Inc. 512K memory device. The circuit was designed into the satellite avionics and is not used to control the satellite. The test consists of writing and reading data with the ferroelectric based memory device. Any errors are detected and are stored on board the satellite. The data is sent to the ground through telemetry once a day. Analysis of the data can determine the kind of error that was found and will lead to a better understanding of the effects of space radiation on memory systems. The test is one of the first flight demonstrations of ferroelectric memory in a near polar orbit which allows testing in a varied radiation environment. The initial data from the test is presented. This paper details the goals and purpose of this experiment as well as the development process. The process for analyzing the data to gain the maximum understanding of the performance of the ferroelectric memory device is detailed

2-Oxoesters: A Novel Class of Potent and Selective Inhibitors of Cytosolic Group IVA Phospholipase A2.

Cytosolic phospholipase A2 (GIVA cPLA2) is the only PLA2 that exhibits a marked preference for hydrolysis of arachidonic acid containing phospholipid substrates releasing free arachidonic acid and lysophospholipids and giving rise to the generation of diverse lipid mediators involved in inflammatory conditions. Thus, the development of potent and selective GIVA cPLA2 inhibitors is of great importance. We have developed a novel class of such inhibitors based on the 2-oxoester functionality. This functionality in combination with a long aliphatic chain or a chain carrying an appropriate aromatic system, such as the biphenyl system, and a free carboxyl group leads to highly potent and selective GIVA cPLA2 inhibitors (X I(50) values 0.00007-0.00008) and docking studies aid in understanding this selectivity. A methyl 2-oxoester, with a short chain carrying a naphthalene ring, was found to preferentially inhibit the other major intracellular PLA2, the calcium-independent PLA2. In RAW264.7 macrophages, treatment with the most potent 2-oxoester GIVA cPLA2 inhibitor resulted in over 50% decrease in KLA-elicited prostaglandin D2 production. The novel, highly potent and selective GIVA cPLA2 inhibitors provide excellent tools for the study of the role of the enzyme and could contribute to the development of novel therapeutic agents for the treatment of inflammatory diseases

Biogeochemical processes at the sediment-water interface in a Northeastern Atlantic abyssal locality (Porcupine Abyssal Plain)

Abstract Temporal and spatial variability in the composition of suspended particulate material (SPM) was found at a Northeastern Atlantic abyssal locality on the Porcupine Abyssal Plain at a depth of 4850 m. Total SPM was distinctly higher in July 1997 than in March-April 1997 and October 1996. The 20 m of water immediately overlying the seabed was strongly influenced by resuspension and the suspended particulate matter was enriched with iron and manganese. Two water distinct layers, which were characterized by being enriched with SPM, Fe p , Mn p and Al p , were encountered during all sampling periods at depths of 4000 m and 4800 m. The enrichment of SPM with biogenic elements such as Ba, Ca and Sr implies it has a high biogenic content. Increases were observed in the concentrations of these biogenic elements throughout the sampling period, which indicates that the biogenic content of the SPM was steadily increased. SPM content and element/aluminum ratios increased in the spring and summer of 1997 compared to autumn 1996, probably as a result of increases in primary productivity, coincidental increases in near-bottom current velocities and possibly other processes such as increases in bacterial activity and bioturbation. Authigenic formation of solid phases of Fe and Mn were also influencing the spatial variability. Chemical reactions involving organic carbon and oxygen, which lead to the incorporation of iron and manganese in various forms into sediments were deduced. It is proposed that there was formation of lepidocrocite (γ-FeOOH) in the presence of organic carbon and oxygen, and also that iron may also have been transformed into Fe 2 O 3 . Re-precipitation of Fe and Mn is strongly enhanced by their enrichment in the SPM in the bottom waters. The sediments predominantly consist of biogenic carbonates and can be characterized as foraminiferal ooze. The proportion of these biogenic carbonates proved to be higher in the more recent sediments, either as result of recent increases in total primary productivity or because of carbonate dissolution within the subsurface sediments. Parallel increases of Mn, Fe and carbonates in the sediment layers supports the idea that the metallic ions are being adsorbed on to calcite surfaces, rather than being co-precipitated in the carbonate phases. Reprecipitation of iron and manganese in other solid phases with the involvement of organic carbon (C org ) in the oxygen penetration zone is suggested

Tactically Extensible and Modular Communications - X-Band TEMCOM-X

This paper will discuss a proposed CubeSat size (3U) telemetry system concept being developed at Marshall Space Flight Center (MSFC) in cooperation with the U.S. Department of the Army and Dynetics Corporation. This telemetry system incorporates efficient, high-bandwidth communications by developing flight-ready, low-cost, Protoflight software defined radio (SDR) and Electronically Steerable Patch Array (ESPA) antenna subsystems for use on platforms as small as CubeSats and unmanned aircraft systems (UASs). The current telemetry system is slightly larger in dimension of footprint than required to fit within a 0.5U CubeSat volume. Extensible and modular communications for CubeSat technologies will partially mitigate current capability gaps between traditional strategic space platforms and lower-cost small satellite solutions. Higher bandwidth capacity will enable high-volume, low error-rate data transfer to and from tactical forces or sensors operating in austere locations (e.g., direct imagery download, unattended ground sensor data exfiltration, interlink communications), while also providing additional bandwidth and error correction margin to accommodate more complex encryption algorithms and higher user volume