Selective flow-induced vesicle rupture to sort by membrane mechanical properties

International audienceVesicle and cell rupture caused by large viscous stresses in ultrasonication is central to biomedical and bioprocessing applications. The flow-induced opening of lipid membranes can be exploited to deliver drugs into cells, or to recover products from cells, provided that it can be obtained in a controlled fashion. Here we demonstrate that differences in lipid membrane and vesicle properties can enable selective flow-induced vesicle break-up. We obtained vesicle populations with different membrane properties by using different lipids (SOPC, DOPC, or POPC) and lipid:cholesterol mixtures (SOPC:chol and DOPC:chol). We subjected vesicles to large deformations in the acoustic microstreaming flow generated by ultrasound-driven microbubbles. By simultaneously deforming vesicles with different properties in the same flow, we determined the conditions in which rupture is selective with respect to the membrane stretching elasticity. We also investigated the effect of vesicle radius and excess area on the threshold for rupture, and identified conditions for robust selectivity based solely on the mechanical properties of the membrane. Our work should enable new sorting mechanisms based on the difference in membrane composition and mechanical properties between different vesicles, capsules, or cells

Conceptual design and analysis of a large antenna utilizing electrostatic membrane management

Conceptual designs and associated technologies for deployment 100 m class radiometer antennas were developed. An electrostatically suspended and controlled membrane mirror and the supporting structure are discussed. The integrated spacecraft including STS cargo bay stowage and development were analyzed. An antenna performance evaluation was performed as a measure of the quality of the membrane/spacecraft when used as a radiometer in the 1 GHz to 5 GHz region. Several related LSS structural dynamic models differing by their stiffness property (and therefore, lowest modal frequencies) are reported. Control system whose complexity varies inversely with increasing modal frequency regimes are also reported. Interactive computer-aided-design software is discussed

Single-Particle Excitations in Narrow Energy Bands

Hubbard\u27s model for studying correlation effects in systems with narrow energy bands is analyzed by means of a technique which allows the calculation of moments of the individual peaks in the spectral weight function for single-particle excitations. The analysis of the zeroth moments of the peaks shows that the total weight in the bands depends on the strength of the kinetic-energy term in the Hamiltonian even though the bands may be narrow and widely separated. This conclusion is illustrated and verified by an exact calculation for the case when there are only two lattice sites. Analysis of first and higher moments yields results for nonmagnetic or paramagnetic phases which are in qualitative agreement with Hubbard\u27s improved solution. However, we find that (a) there occurs a spin-dependent shift in the band energies which has not been obtained by other treatments of the model and which energetically favors ferromagnetism, and (b) single-particle excitations are more heavily damped in antiferromagnetic than in isomorphic paramagnetic phases

A characterization of community fish refuge typologies in rice field fisheries ecosystems

In rural Cambodia, fish is a source of food and income to millions of people. However, there has been a real threat to fish populations in natural wetlands due to the degradation of aquatic biodiversity and habitat, illegal fishing, increase of population and demand for fish, and the use of harmful pesticides for agriculture. The Rice Field Fisheries Enhancement Project (RFFEP) seeks to rebuild and protect the fish populations through innovative methods. The project works with communities to sustainably strengthen the rice field fisheries near their villages by improving protected habitats called "community fish refuges". This handbook characterizes rice field fisheries that are connected to community fish refuges. Community fish refuges are designated fish conservation areas promoted by the Fisheries Administration of the Royal Cambodian Government. It also examines the characteristics of rain-fed rice field ecosystems that are connected to community fish refuges in order to further refine descriptive criteria and better understand potential benefits and management strategies

Progression and assessment in foreign languages at Key Stage 2

The teaching of primary languages has been increasing steadily, in response to the future entitlement for all Key Stage 2 (KS2) pupils aged 7-11 to learn a foreign language by 2010. However, there remain concerns about progression both within KS2 and through to secondary school and about how learners' progress is assessed. This paper presents findings on the issues of progression and assessment taken from case studies which formed part of a project funded by the then Department for Education and Skills (DfES), now the Department for Children, Schools and Families (DCSF). This project set out to evaluate 19 local authority (LA) Pathfinders in England that were piloting the introduction of foreign language learning at KS2 between 2003 and 2005. Findings revealed that there was inconsistency between schools, even within each LA Pathfinder, in the use of schemes of work and that assessment was generally underdeveloped in the majority of the Pathfinders. In order to set these findings in context, this paper examines the issues of progression and assessment in foreign language learning in England. Finally, it investigates the challenges English primary schools face in terms of progression and assessment in the light of the new entitlement and discusses implications for the future. Managing progression, both within KS2 and through to secondary school at KS3 (ages 11-14), is one of the key factors in determining the overall success of starting languages in primary school

Geometrical complexity of data approximators

There are many methods developed to approximate a cloud of vectors embedded in high-dimensional space by simpler objects: starting from principal points and linear manifolds to self-organizing maps, neural gas, elastic maps, various types of principal curves and principal trees, and so on. For each type of approximators the measure of the approximator complexity was developed too. These measures are necessary to find the balance between accuracy and complexity and to define the optimal approximations of a given type. We propose a measure of complexity (geometrical complexity) which is applicable to approximators of several types and which allows comparing data approximations of different types.Comment: 10 pages, 3 figures, minor correction and extensio

Injury to muscle fibres after single stretches of passive and maximally stimulated muscles in mice.

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/110091/1/tjp19954882459.pd

Effectiveness of unsupervised physical activity interventions in people with COPD: a systematic review and meta-analysis

No abstract available.publishe