Evidence of two lineages of metriorhynchid crocodylomorphs in the Lower Cretaceous of the Czech Republic

Metriorhynchid crocodylomorphs were an important component in shallow marine ecosystems during the Middle Jurassic to Early Cretaceous in the European archipelago. While metriorhynchids are well known from western European countries, their central and eastern European record is poor and usually limited to isolated or fragmentary specimens which often hinders a precise taxonomic assignment. However, isolated elements such as tooth crowns, have been found to provide informative taxonomic identifications. Here we describe two isolated metriorhynchid tooth crowns from the upper Valanginian (Lower Cretaceous) of the Stramberk area, Czech Republic. Our assessment of the specimens, including multivariate analysis of dental measurements and surface enamel structures, indicates that the crowns belong to two distinct geosaurin taxa (Plesiosuchina? indet. and Torvoneustes? sp.) with different feeding adaptations. The specimens represent the first evidence of Metriorhynchidae from the Czech Republic and some of the youngest metriorhynchid specimens worldwide.Web of Science66236735

Changement linguistique et périodisation du francąis (pré)classique: Deux études de cas à partir des corpus du RCFC

RÉSUMÉCette étude propose d’explorer et d’identifier des moments particuliers où le changement linguistique se produit, afin de confirmer ou de rejeter l’idée d’une période spécifique désignée par le terme « français préclassique », avec une rupture – ou frontière chronolectale – détectable autour de 1630 (cf. Ayres-Bennett et Caron, 2016). Afin de vérifier dans quelle mesure cette chronologie peut être confirmée, il est nécessaire de multiplier des analyses fines et pointues sur des traits linguistiques qui ont subi des changements à l’époque en question et d’interroger une gamme de textes qui reflètent la variation discursive et pragmatique, au lieu de consulter le canon des traditions textuelles actuellement disponibles sur des bases numérisées, qui sont essentiellement littéraires. C’est pourquoi nous avons consulté des sources de nature différente, qui pourraient attester des usages émergents, à savoir les corpus du Réseau Corpus Français Préclassique et Classique (RCFC). Seront présentés les résultats de deux études de cas (la recatégorisation des formesdedans/dessous/dessus/dehorset la montée des clitiques), abondamment discutés par les remarqueurs.</jats:p

Palaeoenvironments and palaeoceanography changes across the Jurassic/Cretaceous boundary in the Arctic realm: case study of the Nordvik section (north Siberia, Russia)

The Jurassic/Cretaceous transition was accompanied by significant changes in palaeoceanography and palaeoenvironments in the Tethyan Realm, but outside the Tethys such data are very scarce. Here we present results of a study of the most complete section in the Panboreal Superrealm, the Nordvik section. Belemnite d18O data show an irregular decrease from values reaching up to 1.6 in the Middle Oxfordian and from 0.8 to 1.7 in the basal Ryazanian, indicating a prolonged warming. The biodiversity changes were strongly related to sea-level oscillations, showing a relatively low belemnite and high ammonite diversity during sea-level rise, accompanied by a decrease of the macrobenthos taxonomical richness. The most prominent sea-level rise is marked by the occurrence of open sea ammonites with Pacific affinities. Peak abundances of spores and prasinophytes correlate with a negative excursion in organic carbon d13C near the J/K boundary and could reflect blooms of green algae caused by disturbance of the marine ecosystem.Web of Science33art. no. 1971

Palaeoenvironments and palaeoceanography changes across the Jurassic/Cretaceous boundary in the Arctic realm: case study of the Nordvik section (north Siberia, Russia)

The Jurassic/Cretaceous transition was accompanied by significant changes in palaeoceanography and palaeoenvironments in the Tethyan Realm, but outside the Tethys such data are very scarce. Here we present results of a study of the most complete section in the Panboreal Superrealm, the Nordvik section. Belemnite d18O data show an irregular decrease from values reaching up to 1.6 in the Middle Oxfordian and from 0.8 to 1.7 in the basal Ryazanian, indicating a prolonged warming. The biodiversity changes were strongly related to sea-level oscillations, showing a relatively low belemnite and high ammonite diversity during sea-level rise, accompanied by a decrease of the macrobenthos taxonomical richness. The most prominent sea-level rise is marked by the occurrence of open sea ammonites with Pacific affinities. Peak abundances of spores and prasinophytes correlate with a negative excursion in organic carbon d13C near the J/K boundary and could reflect blooms of green algae caused by disturbance of the marine ecosystem.Web of Science33art. no. 1971

Palaeoenvironments and palaeoceanography changes across the Jurassic/Cretaceous boundary in the Arctic realm: case study of the Nordvik section (north Siberia, Russia)

The Jurassic/Cretaceous transition was accompanied by significant changes in palaeoceanography and palaeoenvironments in the Tethyan Realm, but outside the Tethys such data are very scarce. Here we present results of a study of the most complete section in the Panboreal Superrealm, the Nordvik section. Belemnite d18O data show an irregular decrease from values reaching up to 1.6 in the Middle Oxfordian and from 0.8 to 1.7 in the basal Ryazanian, indicating a prolonged warming. The biodiversity changes were strongly related to sea-level oscillations, showing a relatively low belemnite and high ammonite diversity during sea-level rise, accompanied by a decrease of the macrobenthos taxonomical richness. The most prominent sea-level rise is marked by the occurrence of open sea ammonites with Pacific affinities. Peak abundances of spores and prasinophytes correlate with a negative excursion in organic carbon d13C near the J/K boundary and could reflect blooms of green algae caused by disturbance of the marine ecosystem.Web of Science33art. no. 1971

E2/E3-independent ubiquitin-like protein conjugation by Urm1 is directly coupled to cysteine persulfidation.

Post-translational modifications by ubiquitin-like proteins (UBLs) are essential for nearly all cellular processes. Ubiquitin-related modifier 1 (Urm1) is a unique UBL, which plays a key role in tRNA anticodon thiolation as a sulfur carrier protein (SCP) and is linked to the noncanonical E1 enzyme Uba4 (ubiquitin-like protein activator 4). While Urm1 has also been observed to conjugate to target proteins like other UBLs, the molecular mechanism of its attachment remains unknown. Here, we reconstitute the covalent attachment of thiocarboxylated Urm1 to various cellular target proteins in vitro, revealing that, unlike other known UBLs, this process is E2/E3-independent and requires oxidative stress. Furthermore, we present the crystal structures of the peroxiredoxin Ahp1 before and after the covalent attachment of Urm1. Surprisingly, we show that urmylation is accompanied by the transfer of sulfur to cysteine residues in the target proteins, also known as cysteine persulfidation. Our results illustrate the role of the Uba4-Urm1 system as a key evolutionary link between prokaryotic SCPs and the UBL modifications observed in modern eukaryotes

Uremic solutes and risk of end stage renal disease in type 2 diabetes

Here we studied plasma metabolomic profiles as determinants of progression to ESRD in patients with Type 2 diabetes (T2D). This nested case-control study evaluated 40 cases who progressed to ESRD during 8-12 years of follow-up and 40 controls who remained alive without ESRD from the Joslin Kidney Study cohort. Controls were matched with cases for baseline clinical characteristics; although controls had slightly higher eGFR and lower levels of urinary albumin excretion than T2D cases. Plasma metabolites at baseline were measured by mass spectrometry-based global metabolomic profiling. Of the named metabolites in the library, 262 were detected in at least 80% of the study patients. The metabolomic platform recognized 78 metabolites previously reported to be elevated in ESRD (uremic solutes). Sixteen were already elevated in the baseline plasma of our cases years before ESRD developed. Other uremic solutes were either not different or not commonly detectable. Essential amino acids and their derivatives were significantly depleted in the cases, whereas certain amino acid-derived acylcarnitines were increased. All findings remained statistically significant after adjustment for differences between study groups in albumin excretion rate, eGFR or HbA1c. Uremic solute differences were confirmed by quantitative measurements. Thus, abnormal plasma concentrations of putative uremic solutes and essential amino acids either contribute to progression to ESRD or are a manifestation of an early stage(s) of the disease process that leads to ESRD in T2D

Mutations in HNF1A Result in Marked Alterations of Plasma Glycan Profile

A recent genome-wide association study identified hepatocyte nuclear factor 1-α (HNF1A) as a key regulator of fucosylation. We hypothesized that loss-of-function HNF1A mutations causal for maturity-onset diabetes of the young (MODY) would display altered fucosylation of N-linked glycans on plasma proteins and that glycan biomarkers could improve the efficiency of a diagnosis of HNF1A-MODY. In a pilot comparison of 33 subjects with HNF1A-MODY and 41 subjects with type 2 diabetes, 15 of 29 glycan measurements differed between the two groups. The DG9-glycan index, which is the ratio of fucosylated to nonfucosylated triantennary glycans, provided optimum discrimination in the pilot study and was examined further among additional subjects with HNF1A-MODY (n = 188), glucokinase (GCK)-MODY (n = 118), hepatocyte nuclear factor 4-α (HNF4A)-MODY (n = 40), type 1 diabetes (n = 98), type 2 diabetes (n = 167), and nondiabetic controls (n = 98). The DG9-glycan index was markedly lower in HNF1A-MODY than in controls or other diabetes subtypes, offered good discrimination between HNF1A-MODY and both type 1 and type 2 diabetes (C statistic ≥ 0.90), and enabled us to detect three previously undetected HNF1A mutations in patients with diabetes. In conclusion, glycan profiles are altered substantially in HNF1A-MODY, and the DG9-glycan index has potential clinical value as a diagnostic biomarker of HNF1A dysfunction