An optimized quantitative proteomics method establishes the cell type-resolved mouse brain secretome

To understand how cells communicate in the nervous system, it is essential to define their secretome, which is challenging for primary cells because of large cell numbers being required. Here, we miniaturized secretome analysis by developing the high-performance secretome protein enrichment with click sugars (hiSPECS) method. To demonstrate its broad utility, hiSPECSwas used to identify the secretory response of brain slices uponLPS-induced neuroinflammation and to establish the cell type-resolved mouse brain secretome resource using primary astrocytes, microglia, neurons, and oligodendrocytes. This resource allowed mapping the cellular origin ofCSFproteins and revealed that an unexpectedly high number of secreted proteinsin vitroandin vivoare proteolytically cleaved membrane protein ectodomains. Two examples are neuronally secretedADAM22 andCD200, which we identified as substrates of the Alzheimer-linked proteaseBACE1. hiSPECSand the brain secretome resource can be widely exploited to systematically study protein secretion and brain function and to identify cell type-specific biomarkers forCNSdiseases

Next-generation sequencing for diagnosis of thoracic aortic aneurysms and dissections: diagnostic yield, novel mutations and genotype phenotype correlations

Additional file 1. Complementary data on primer sequences, SKI amplification and survival analyses

Many Labs 5:Testing pre-data collection peer review as an intervention to increase replicability

Replication studies in psychological science sometimes fail to reproduce prior findings. If these studies use methods that are unfaithful to the original study or ineffective in eliciting the phenomenon of interest, then a failure to replicate may be a failure of the protocol rather than a challenge to the original finding. Formal pre-data-collection peer review by experts may address shortcomings and increase replicability rates. We selected 10 replication studies from the Reproducibility Project: Psychology (RP:P; Open Science Collaboration, 2015) for which the original authors had expressed concerns about the replication designs before data collection; only one of these studies had yielded a statistically significant effect (p < .05). Commenters suggested that lack of adherence to expert review and low-powered tests were the reasons that most of these RP:P studies failed to replicate the original effects. We revised the replication protocols and received formal peer review prior to conducting new replication studies. We administered the RP:P and revised protocols in multiple laboratories (median number of laboratories per original study = 6.5, range = 3?9; median total sample = 1,279.5, range = 276?3,512) for high-powered tests of each original finding with both protocols. Overall, following the preregistered analysis plan, we found that the revised protocols produced effect sizes similar to those of the RP:P protocols (?r = .002 or .014, depending on analytic approach). The median effect size for the revised protocols (r = .05) was similar to that of the RP:P protocols (r = .04) and the original RP:P replications (r = .11), and smaller than that of the original studies (r = .37). Analysis of the cumulative evidence across the original studies and the corresponding three replication attempts provided very precise estimates of the 10 tested effects and indicated that their effect sizes (median r = .07, range = .00?.15) were 78% smaller, on average, than the original effect sizes (median r = .37, range = .19?.50)

Assessment of Cutting Edge Defects Using a Vision Method

The paper discusses a vision method of assessment of laser cutting edges and surfaces of metals. It presents an analysis of the defects that occur at the edges and surfaces of components cut out in laser technology. It discusses in detail examples of defects, their causes, and methods of preventing their occurrence in the cutting process. The paper presents a vision method for the identification and assessment of defects based on selected examples. It also discusses a method of constructing a three-dimensional image of a product, issues related to the resolution of the vision system and the resolution of laser beam analysis, and methods of image analysis

Evaluation of the Operational Parameters Of Ropes

This article discusses a vision method of measuring the geometric parameters of ropes and evaluating their wear based on measurements made in a three-dimensional rope image. The article discusses the method of construction of a three-dimensional image based on mapping of the actual dimensions of the rope and on algorithms that allow determination of the parameters describing its basic geometrical dimensions and surface condition. The article discusses issues related to resolution of the vision system, resolution of laser beam analysis, and resolution relating to the measurement of the height profile on the surface of the rope. Based on the image constructed in such a way, measurements are presented in order to assess the dimensional parameters and surface defects in sample rope structures. Based on tests and analyses of the three-dimensional image, a range of inspection tasks using 3D vision systems is indicated

The method of building 3d product image using vision system

Artykuł przedstawia możliwości wykorzystania trójwymiarowego systemu wizyjnego w procesie budowy obrazu 3D produktu prowadzonym bezpośrednio na linii technologicznej np. w zakładach wytwarzających elementy pneumatyki lub hydrauliki. W pracy prezentowana jest metoda doboru odpowiedniej geometrii systemu, w skład którego wchodzi kamera i oświetlenie laserowe. Omówiono metodę pobierania profili wysokości i budowy na ich podstawie obrazu 3D produktu. Szczegółowo prezentowany jest wpływ doboru geometrii na rozdzielczość systemu wizyjnego. W pracy prezentowana jest również metoda obróbki obrazu umożliwiająca tworzenie profilu wysokości tj. przekroju produktu.The article discusses of using possibility threedimensional vision system in process of 3D product image building. The method of selecting suitable geometry for vision system based on camera and laser lighting is presented. The influence of geometry selection on resolution in vision system and the method of image processing are presented for creating the profile of height in product section

Zastosowanie zaawansowanych metod optycznych w pomiarach własności stopów z pamięcią kształtu

Shape Memory Alloys are called smart or advanced materials because of their special features which are very useful in the latest solutions. Therefore, it is very important to gain a good knowledge of all these features for the purposes of modeling, describing, and ultimately controlling the behavior of these materials. This paper describes how to measure selected properties of SMA. It describes how to measure real temperature and diameter of thin SMA wires. At the end, we use a high speed camera to find out how an SMA wire breaks during stretching.Stopy z pamięcią kształtu (Shape Memory Alloys - SMA) nazywane są zaawansowanymi materiałami ze względu na ich specjalne własności, które są chętnie stosowane w nowoczesnych rozwiązaniach technicznych. Identyfikacja ich w celu lepszego poznania, modelowania a także sterowania ich parametrami jest niezbędna we właściwym stosowaniu tych materiałów. W artykule opisano, jak zmierzyć rzeczywistą temperaturę i średnicę cienkiego drutu SMA z wykorzystaniem kamery termowizyjnej i mikrometru laserowego. Opisano również badania z użyciem kamery HSC zjawisk zachodzących w trakcie zerwania drutu SMA