EFEKTIFITAS PENERAPAN MODEL KOMUNIKASI PENYULUHAN DALAM AGRIBISNIS JAGUNG DI TIMOR BARAT

This study aims to determine the effectiveness of the communication model applied and to know the relationship between socio-economic factors and the effectiveness of communication in corn agribusiness. The location of the study was determined purposively (sampling) of Oeteta and Karang Sirih villages with the consideration that both villages have dry land and corn production centers in both districts. The respondent taken from the farmers who grow corn at Oeteta and Karang Sirih villages. The respondent were chosen by simple random sampling of 124 respondent. Data was analized descriptive and use Rank Spearman Correlation. The results are: 1) the effectiveness application of communication in corn agribusiness West Timor was moderate category, with the average score 2.79 or 55.81%.  2) education, proneness of innovation, adapter category and family size were significantly correlated to the effectiveness of communication

Olive growing in the arid valleys of Northwest Argentina (provinces of Catamarca, La Rioja and San Juan)

En este artículo se describe la situación del olivar en las provincias argentinas de Catamarca, La Rioja y San Juan

Kajian Faktor Sosial Ekonomi terhadap Respon Petani dalam Menerapkan Inovasi Double Track untuk Meningkatkan Ketahanan Pangan Petani pada Masa Pandemi Covid-19

Penelitian ini telah dilaksanakan pada akhir tahun 2022,  bertujuan mengetahui  factor social ekonomi berkorelasi dengan  respon petani dalam penerapan  inovasi Double Track pada tanaman jagung. Sebanyak 83 petani dipilih secara acak. Pengumpulan data menggunakan metode survei.  Data dianalisis dengan menggunakan korelasi Rank Spearman dengan sistem komputasi SPPS 26. Hasilnya adalah faktor berkorelasi nyata dengan respon petani adalah pendidikan formal, pendidikan nonformal, dan luas lahan.. Dibutuhkan perhatian pemerintah untuk peningkatan kegiatan  penyuluhan dan pendampingan dengan metode gabungan yang terdiri dari ceramah, diskusi, pelatihan partisipatif, SLP dan konsultasi pribadi tentang sistem ‘double track’.Sebaliknya, faktor umur, pengalaman berusahatani dan jumlah tanggungan keluarga tidak memiliki korelasi dengan respon petani

ANALISIS HUBUNGAN ANTARA FAKTOR SOSIAL EKONOMI DAN TINGKAT KEDINAMISAN ANGGOTA KELOMPOK TANI

This research aimed to assess dynamics level of the members of the Harapan Makmur farmers' group in the village of Tuatuka, East Kupang District, Kupang Regency and the relationship between socio-economic factors (age, formal education, non-formal education, number of family- dependent people, income, agricultural experience) with the dynamics level of farmer group members of Harapan Makmur Tuatuka Sub-District, East Kupang District, Kupang Regency. This research used the case study method. The sample used is all farmers who are members of prosperous farmer groups of 35 respondents. Analysis of the data used in this research used a Likert scale and the Sperman rank correlation test (rs). The results showed that the age of majority respondents was classified as productive (85.70%), formal education was classified as low (60.00%), non-formal education was classified as high (91.43%), the number of dependent persons was quite high (62.86)%), long experience in agriculture (37.14%) and agricultural income wass quite high (88.58%). The dynamic level of the members of Harapan Makmur farmer group in Tuatuka subdistrict, East Kupang district, Kupang Regency was in the dynamic category (68.0%). ), The results of the analysis (rs) showed that socio- economic factors (age, formal education, non-formal education, number of family members, income,agricultural experience) that were related to the dynamics of the farmer group members were non- formal education. While other socio-economic factors had no real connection with the dynamics of Harapan Makmur farmer group in the village of Tuatuka, East Kupang District, Kupang Regency

Impact of DNA methylation on trophoblast function

The influence of epigenetics is evident in many fields of medicine today. This is also true in placentology, where versatile epigenetic mechanisms that regulate expression of genes have shown to have important influence on trophoblast implantation and placentation. Such gene regulation can be established in different ways and on different molecular levels, the most common being the DNA methylation. DNA methylation has been shown today as an important predictive component in assessing clinical prognosis of certain malignant tumors; in addition, it opens up new possibilities for non-invasive prenatal diagnosis utilizing cell-free fetal DNA methods. By using a well known demethylating agent 5-azacytidine in pregnant rat model, we have been able to change gene expression and, consequently, the processes of trophoblast differentiation and placental development. In this review, we describe how changes in gene methylation effect trophoblast development and placentation and offer our perspective on use of trophoblast epigenetic research for better understanding of not only placenta development but cancer cell growth and invasion as well

Modification of conservative treatment of heterotopic cervical pregnancy by Foley catheter balloon fixation with cerclage sutures at the level of the external cervical os: a case report

<p>Abstract</p> <p>Introduction</p> <p>Conservative treatment of a heterotopic cervical pregnancy was performed with a modification of the fixation of a Foley catheter at the level of the external cervical os, followed by the ligature of the descending cervical branches of the uterine arteries and systemic methotrexate application.</p> <p>Case presentation</p> <p>A 34-year-old Caucasian woman was diagnosed with double gestation after 6 weeks of <it>in vitro </it>fertilization treatment. A gynecological examination and color Doppler ultrasound scan revealed intra-uterine and cervical gestational sacs both containing live fetuses. A Foley catheter balloon was inserted into the cervical canal, inflated and fixed by a cerclage suture at the level of the external cervical os, followed by ligation of the descending cervical branches of the uterine arteries. Systemic methotrexate was applied. Three days after removal of the Foley catheter, an evacuation of the intra-uterine gestational sac was performed. Hemorrhage from the implantation site was controlled immediately and a pregnancy termination was successfully performed. The procedure was uneventful and our patient was discharged with a preserved uterus.</p> <p>Conclusions</p> <p>Conservative treatment of cervical pregnancy using a Foley catheter balloon is more efficacious if the Foley catheter balloon is attached in the correct position with a cerclage suture at the level of the external os, followed by ligation of the descending cervical branches of the uterine arteries, thereby exerting maximal pressure on the bleeding vessels.</p

Discovery of DNA methylation markers in cervical cancer using relaxation ranking

<p>Abstract</p> <p>Background</p> <p>To discover cancer specific DNA methylation markers, large-scale screening methods are widely used. The pharmacological unmasking expression microarray approach is an elegant method to enrich for genes that are silenced and re-expressed during functional reversal of DNA methylation upon treatment with demethylation agents. However, such experiments are performed in <it>in vitro </it>(cancer) cell lines, mostly with poor relevance when extrapolating to primary cancers. To overcome this problem, we incorporated data from primary cancer samples in the experimental design. A strategy to combine and rank data from these different data sources is essential to minimize the experimental work in the validation steps.</p> <p>Aim</p> <p>To apply a new relaxation ranking algorithm to enrich DNA methylation markers in cervical cancer.</p> <p>Results</p> <p>The application of a new sorting methodology allowed us to sort high-throughput microarray data from both cervical cancer cell lines and primary cervical cancer samples. The performance of the sorting was analyzed <it>in silico</it>. Pathway and gene ontology analysis was performed on the top-selection and gives a strong indication that the ranking methodology is able to enrich towards genes that might be methylated. Terms like regulation of progression through cell cycle, positive regulation of programmed cell death as well as organ development and embryonic development are overrepresented. Combined with the highly enriched number of imprinted and X-chromosome located genes, and increased prevalence of known methylation markers selected from cervical (the highest-ranking known gene is <it>CCNA1</it>) as well as from other cancer types, the use of the ranking algorithm seems to be powerful in enriching towards methylated genes.</p> <p>Verification of the DNA methylation state of the 10 highest-ranking genes revealed that 7/9 (78%) gene promoters showed DNA methylation in cervical carcinomas. Of these 7 genes, 3 (<it>SST</it>, <it>HTRA3 </it>and <it>NPTX1</it>) are not methylated in normal cervix tissue.</p> <p>Conclusion</p> <p>The application of this new relaxation ranking methodology allowed us to significantly enrich towards methylation genes in cancer. This enrichment is both shown <it>in silico </it>and by experimental validation, and revealed novel methylation markers as proof-of-concept that might be useful in early cancer detection in cervical scrapings.</p

Gene deletion of P-Selectin and ICAM-1 does not inhibit neutrophil infiltration into peritoneal cavity following cecal ligation-puncture

BACKGROUND: Neutrophil infiltration is one of the critical cellular components of an inflammatory response during peritonitis. The adhesion molecules, P-selectin and intercellular adhesion molecule (ICAM)-1, mediate neutrophil-endothelial cell interactions and the subsequent neutrophil transendothelial migration during the inflammatory response. Despite very strong preclinical data, recent clinical trials failed to show a protective effect of anti-adhesion therapy, suggesting that the length of injury might be a critical factor in neutrophil infiltration. Therefore, the objective of this study was to determine the role of P-selectin and ICAM-1 in neutrophil infiltration into the peritoneal cavity during early and late phases of peritonitis. METHODS: Peritonitis was induced in both male wild-type and P-selectin/ICAM-1 double deficient (P/I null) mice by cecal ligation-puncture (CLP). Peripheral blood and peritoneal lavage were collected at 6 and 24 hours after CLP. The total leukocyte and neutrophil contents were determined, and neutrophils were identified with the aid of in situ immunohistochemical staining. Comparisons between groups were made by applying ANOVA and student t-test analysis. RESULTS: CLP induced a severe inflammatory response associated with a significant leukopenia in both wild-type and P/I null mice. Additionally, CLP caused a significant neutrophil infiltration into the peritoneal cavity that was detected in both groups of mice. However, neutrophil infiltration in the P/I null mice at 6 hours of CLP was significantly lower than the corresponding wild-type mice, which reached a similar magnitude at 24 hours of CLP. In contrast, in peritonitis induced by intraperitoneal inoculation of 2% glycogen, no significant difference in neutrophil infiltration was observed between the P/I null and wild-type mice at 6 hours of peritonitis. CONCLUSIONS: The data suggest that alternative adhesion pathway(s) independent of P-selectin and ICAM-1 can participate in neutrophil migration during peritonitis and that the mode of stimuli and duration of the injury modulate the neutrophil infiltration

Performance of an endcap prototype of the ATLAS accordion electromagnetic calorimeter

The design and construction of a lead-liquid argon endcap calorimeter prototype using an accordion geometry and conceived as a sector of the inner wheel of the endcap calorimeter of the future ATLAS experiment at the LHC is described. The performance obtained using electron beam data is presented. The main results are an energy resolution with a sampling term below $11\%/\sqrt{E(\rm GeV)}$ and a small local constant term, a good linearity of the response with the incident energy and a global constant term of 0.8\% over an extended area in the rapidity range of $2.2 < \eta <2.9$. These properties make the design suitable for the ATLAS electromagnetic endcap calorimeter