70 research outputs found
Agronomic Characterisation of Sewage Sludge: Residual Effects
The progressive implementation of the EU Urban Waste Water Treatment Directive 91/271/EEC is increasing the quantities of sewage sludge requiring disposal. Agronomic use of sewage sludge should be based on crop fertiliser requirements and heavy metals content. Sewage sludge fertiliser potential depends on the stabilisation treatments of sewage sludge: composting, anaerobic digestion, aerobic digestion or pelleting (EPA, 1994). The objective of this experiment was to evaluate the fertiliser effectiveness and residual effects of three types of sewage sludge treated in three different ways
Shading Effect on Production and Protein Concentration of \u3cem\u3eDactylis Glomerata\u3c/em\u3e and \u3cem\u3eAgrostis Tenuis\u3c/em\u3e
Silvopastoral systems make compatible livestock and timber production and provide important advantages from economic and ecological points of view (Sibbald, 1996). Around one million ha of new afforested areas promoted by the EU Common Agricultural Policy have been established in the last decade, that can be used as potential silvopastoral system areas. Pasture production is usually reduced in dense stands as trees grow up due to the light interception by the tree crown, but the radiation reaching the soil will depend on the tree type and this will affect herbaceous species composition and development. The aim of this work was to evaluate the shading effect (0 and 50 % of light interception) on pasture production and composition of monocultures of cocksfoot (Dactylis glomerata L. var. Artabro) and bent grass (Agrostis tenuis Sibth. cv Highland) in simulated conditions
Silvopastoral systems established with Pinus radiate D. Don and Betula pubescens Ehrh.: Tree growth, understorey biomass and vascular plant biodiversity
In the European Union, the majority of afforestation is carried out on former agricultural land. This afforestation causes fundamental changes in ecosystem structure and functioning, with the trees intercepting light and precipitation, producing litterfall and competing for soil nutrients. All of these effects could potentially have a negative impact on understorey biomass and vascular plant biodiversity in a relatively short time span. This study aims to evaluate the effects of afforestation with Pinus radiata D. Don (Monterey pine: pine) and Betula pubescens Ehrh. (Downy birch: birch), established at two different densities (2500 and 833 trees ha21) and sown with two different pasture mixtures (Dactylis glomerata L. + Trifolium repens L. + Trifolium pratense L. and Lolium perenne L. + T. repens L. + T. pratense L.), on understorey biomass, alpha plant biodiversity, life cycle type (annuals vs. perennials) and beta biodiversity over a period of 11 years. Pine showed better development than birch throughout the study, although both species were very sensitive to tree density. While increasing density increased pine height and decreased pine diameter, both aspects of birch development were reduced by higher tree densities. Aboveground biomass increased throughout the study. Alpha plant biodiversity was drastically reduced under pine established at high density, 11 years after afforestation. However, the similarities in species richness, species composition and the low beta growth rates found under pine at low density, and birch at both high and low density suggest that, in these cases, the choice of canopy tree has little consequence for understorey biodiversity at this stage of system development.info:eu-repo/semantics/publishedVersio
Effect of Tree Species and Density on Pasture Production in Galicia, Spain
Galicia produces 50% of the forest products of Spain. Livestock production earns 62% of the income of the agrarian sector in Galicia. Afforestation has been very important in the last decade to such an extent that the area of forest and woodlands now covers 62% of Galicia. It is necessary to increase the rate of return on investments in planted forests in order to avoid rural depopulation through improvement of rural development and welfare. This paper reports on the effect of combining pastures with trees
Seasonal Variation of Crude Protein Content of Different Herbaceous, Shrub and Tree Species
Silvopastoralism is a sustainable way of land management that reduces fire risk due to the reduction of fuel under trees when plants are used as animal food. This is particularly important in areas like Galicia that have 16% of the fired area of Europe. Silvopastoral systems can contribute to environment conservation and provide feed for autochthonous breeds more adapted to mountain conditions, enhancing biodiversity conservation. In formation on seasonal changes in crude protein content of spontaneous species will indicate better management of pasture resources in mountain areas
Pasture Production After Sewage Sludge and Liming Application on Highlands in North West Spain
In recent years, a sewage sludge surplus has been created in the EU countries, due to the Urban Waste Water Treatment Directive 91/271/CEE. Therefore, it is necessary to find adequate disposal for these residues in accordance with EU policy. Organic matter and nutrient sewage sludge contain principally N, indicating that it could be used as fertiliser. The main risk of this residue is its heavy metal content, whose solubility is usually increased as soil pH declines. The objective of this experiment was to determine the effect of liming and sewage sludge application on pasture production in a silvopastoral system located on acid soi
Modeling Pinus radiata D. Don growth and pasture production under different land uses and climate scenarios
Yield-SAFE is a biophysical model to predict long-term production according to light and water availability in agricultural, forest, and agroforestry systems. The Yield-SAFE model should be calibrated and validated for the highest number of tree species and crops to be used as a management tool that takes into account climate change. This study aimed to calibrate and validate the Yield-SAFE model for Pinus radiata D. Don and sown pasture (Dactylis glomerata L.) to estimate the production in (1) forest systems, (2) agricultural systems, and (3) silvopastoral systems established in Galicia (NW Spain) under different conditions of climate: (i) reference “current” climate from 1961 to 1990, (ii) climate from 2021 to 2050, and (iii) climate from 2051 to 2080. The Yield-SAFE model can now be used to assess the long-term productivity of P. radiata D. Don and D. glomerata L. under different land uses and climate conditions. The Yield-SAFE model simulated similar tree and pasture growth in all scenarios of climate because the inter-annual variation of climate was small. However, tree growth estimated with the Yield-SAFE model was higher in the silvopastoral systems than in the forest systems, indicating that land use had more impact on land productivity than climate. Therefore, in regions such as Galicia, the Yield-SAFE model could be used as a tool to support the land use change in an agroforestry context, whilst also including climate scenarios which is considered a valuable solution to mitigate the effect of climate change
Organic farming in northeast of Portugal: effects of soil fertility management on DM yield and nutrients composition of pastures
The aim of this work was to evaluate the effect of six types of soil fertility management: no fertiliser
(NF), lime inputs (Ca), mineral fertilisation combined with hming (CaP (lime and phosphorous), CaPB
(lime, phosphorous and boron), manure (M), and manure+lime+mineral fertilisation (MCaPB), and two
types of pasture (unsown and sown) on DM yield, botanical composition and nutrients content of pasture
during two years of study. DM yield was significantly increased when plots were fertilised with manure
(M and MCaPB), which also improved the proportion of legumes, mainly in the sown pasture
Vocabulario forestal [galego-español-inglés]
Deputación de Lugo. Vicepresidencia Primeir
Hakai reduces cell-substratum adhesion and increases epithelial cell invasion
[Abstract]
Background. The dynamic regulation of cell-cell adhesions is crucial for developmental processes, including tissue formation, differentiation and motility. Adherens junctions are important components of the junctional complex between cells and are necessary for maintaining cell homeostasis and normal tissue architecture. E-cadherin is the prototype and best-characterized protein member of adherens junctions in mammalian epithelial cells. Regarded as a tumour suppressor, E-cadherin loss is associated with poor prognosis in carcinoma. The E3 ubiquitin-ligase Hakai was the first reported posttranslational regulator of the E-cadherin complex. Hakai specifically targetted E-cadherin for internalization and degradation and thereby lowered epithelial cell-cell contact. Hakai was also implicated in controlling proliferation, and promoted cancer-related gene expression by increasing the binding of RNA-binding protein PSF to RNAs encoding oncogenic proteins. We sought to investigate the possible implication of Hakai in cell-substratum adhesions and invasion in epithelial cells.
Methods. Parental MDCK cells and MDCK cells stably overexpressing Hakai were used to analyse cell-substratum adhesion and invasion capabilities. Western blot and immunofluoresecence analyses were performed to assess the roles of Paxillin, FAK and Vinculin in cell-substratum adhesion. The role of the proteasome in controlling cell-substratum adhesion was studied using two proteasome inhibitors, lactacystin and MG132. To study the molecular mechanisms controlling Paxillin expression, MDCK cells expressing E-cadherin shRNA in a tetracycline-inducible manner was employed.
Results. Here, we present evidence that implicate Hakai in reducing cell-substratum adhesion and increasing epithelial cell invasion, two hallmark features of cancer progression and metastasis. Paxillin, an important protein component of the cell-matrix adhesion, was completely absent from focal adhesions and focal contacts in Hakai-overexpressing MDCK cells. The expression of Paxillin was found to be regulated by a proteasome-independent mechanism, possibly due to the decreased abundance of E-cadherin.
Conclusions. Taken together, these results suggest that Hakai may be involved in two hallmark aspects of tumour progression, the lowering cell-substratum adhesion and the enhancement of cell invasion.Xunta de Galicia; PS09/24Xunta de Galicia; 10CSA916023P
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