Microfluidic method for rapid turbidimetric detection of the DNA of Mycobacterium tuberculosis using loop-mediated isothermal amplification in capillary tubes

We describe a microfluidic method for rapid isothermal turbidimetric detection of the DNA of Mycobacterium tuberculosis. Loop-mediated isothermal amplification is accomplished in capillary tubes for amplifying DNA in less than 15 min, and sensitivity and specificity were compared to conventional loop-mediated isothermal amplification (LAMP). The method can detect as little as 1 pg mL−1 DNA in a sample. Results obtained with clinical specimens indicated 90 % sensitivity and 95 % specificity for microfluidic LAMP in comparison to culture methods. No interference occurred due to the presence of nonspecific DNAs. The findings demonstrate the power of the new microfluidic LAMP test for rapid molecular detection of microorganisms even when using bare eyes. © 2014, Springer-Verlag Wien

Effect of Electrodeposition Frequency on Magnetic Properties of (Co0.97Al0.03)0.7Fe0.3 Nanowires

The magnetic properties of (Co0.97Al0.03)0.7Fe0.3 nanowires electrodeposited at different frequency of 50- 1000 Hz were studied. This nanowires were prepared by alternative current electrodeposition using porous anodic aluminum oxide as template. The results show that coercivity of samples slightly increased with increasing the frequency. Comparing the M–H hysteresis loops in different frequency shows that saturation magnetization (Ms) per unit area decreases with increasing the frequency. Magnetic properties were also investigated after annealing at 575 C. After annealing, a clear increase in coercivity was observed. When you are citing the document, use the following link http://essuir.sumdu.edu.ua/handle/123456789/3534

Effect of Current Frequency and Annealing on Magnetic Properties of [Co70Fe30]97Sn3 Nanowire Arrays

[Co70Fe30]97Sn3 alloy nanowire arrays have been fabricated by alternative current (ac) electrodeposition of Co2+, Fe2+ and Sn2+ into anodic aluminum oxide (AAO) templates. The samples were deposited at current frequency 50, 100, 200, 300, 400, 500, 800 and 1000 Hz, respectively. The structure and magnetic properties of [Co70Fe30]97Sn3 nanowire arrays dependence on different current frequency were analyzed by XRD and alternating gradient field magnetometer (AGFM). The X-ray diffraction patterns are shown an amorphous structure of the[Co70Fe30]97Sn3 nanowires. When you are citing the document, use the following link http://essuir.sumdu.edu.ua/handle/123456789/3535

Parámetros cinéticos y termodinámicos de la curcumina en aceites comestibles con diferentes grados de insaturación

The antioxidant activity of curcumin (0.02-0.1%) was evaluated in olive, sesame, and safflower oils at 373, 383, and 393 K. The results were examined in contrast to the effects of tocopherol (0.1%) and BHT (0.02%), so that the inhibitory function of curcumin was evaluated comparatively. The activation energy of oxidation was determined for olive (82.94 kJ·mol-1), sesame (77.39 kJ·mol-1) and safflower oils (74.42 kJ·mol-1). Adding curcumin (0.1%) enhanced the activation energy by 26.26, 26.64, and 38.81% in the case of olive, safflower, and sesame oils, respectively. Based on Gibbs free energy, curcumin functioned more effectively in olive oil at 373 K (growth coefficient: 1.52%), compared to the action of the other two antioxidants, namely tocopherol (1.43%) and BHT (1.39%). The efficiency of curcumin was lower in oils which had a higher degree of polyunsaturation due to the disproportionation of the hydrogen-donating mechanism and the rate of free-radical formation in these oils.La actividad antioxidante de la curcumina (0,02-0,1 %) se evaluó en aceites de oliva, sésamo y cártamo a 373, 383 y 393 K. Los resultados se contrastaron con los efectos del tocoferol (0,1 %) y del BHT (0,02%), por lo que se evaluó comparativamente la función inhibitoria de la curcumina. Se determinó la energía de activación de la oxidación para los aceites de oliva (82,94 kJ·mol-1), sésamo (77,39 kJ·mol-1) y cártamo (74,42 kJ·mol-1). La adición de curcumina (0,1 %) mejoró la energía de activación en un 26,26 %, 26,64 % y 38,81 % en el caso de los aceites de oliva, cártamo y sésamo, respectivamente. Según la energía libre de Gibbs, la curcumina funcionó de manera más eficaz en aceite de oliva a 373 K (coeficiente de crecimiento: 1,52 %), en comparación con la acción de los otros dos antioxidantes; es decir, tocoferol (1,43 %) y BHT (1,39 %). La eficiencia de la curcumina fue menor en los aceites que tenían un mayor grado de poliinsaturación debido a la desproporción del mecanismo de donación de hidrógeno y la tasa de formación de radicales libres en estos aceites

Pyromellitic dianhydride crosslinked cyclodextrin nanosponges for curcumin controlled release; formulation, physicochemical characterization and cytotoxicity investigations

Aim: In this study, a nanosponge structure was synthesised with capability of encapsulating curcumin as a model polyphenolic compound and one of the herbal remedies that have widely been considered due to its ability to treat cancer. Methods: FTIR, DSC and XRD techniques were performed to confirm the formation of the inclusion complex of the nanosponge-drug. Results: DSC and XRD patterns showed an increasing stability and a decreasing crystallinity of curcumin after formation of inclusion complex. Encapsulation efficiency was 98% (w/w) and a significant increase was observed in loading capacity (184% w/w). The results of cytotoxicity assessments demonstrated no cell toxicity on the healthy cell line, while being toxic against cancer cells. Haemolysis test was performed to evaluate the blood-compatibility characteristic of nanosponge and complex and the results showed 0.54% haemolysis in the lowest complex concentration (50μgml−1) and 5.09% at the highest concentration (200μgml−1). Conclusions: Thus, the introduced system could be widely considered in cancer treatment as a drug delivery system

Mass rearing of the larvae of Chironomus riparius (Dip.: Chironomidae)

Aquatic larvae of chironomid midge, Chironomus riparius Meigen, are one of the richest diets in fish rearing systems. The quantity and quality of food used to rearing these larvae, affect this developmental stage. This study was conducted to determine the effect of different diets (chicken manure, rice bran and soybean meal) with different weights (1, 3 and 5 gr) on larval development, the best time of harvesting at different constant temperatures (16-20, 22 ± 1, 24 ± 1, 26 ± 1, 28 ± 1 and 30 ± 1 oC) and the yield (larval live weight (gr) m-2 per week) based on the number of reared egg masses with the favorable diet. Results indicated that the highest weight of harvested larvae obtained when they fed on 5 gr of chicken manure (3.53 ï± 0.4, p < 0.01). The last larval instars were harvested at the shortest time (on 13th days), when reared at 24 ± 1 or 26 ± 1 oC feeding on chicken manure (compared to other temperatures). Mean yield of 131.38 ï± 13.99 gr m-2 per week obtained, when five egg masses were reared at 26 ± 1 oC on the chicken manure

Review of synthesis, characteristics and technical challenges of biodiesel based drilling fluids

Acknowledgements The Egyptian authors were supported by Science, Technology & Innovation Funding Authority (STDF) under the grant (30894). The UK's authors would like to acknowledge the financial support from an Institutional Links grant, ID 352343681, under the Newton-Mosharafa Fund partnership. The grant is funded by the UK Department for Business, Energy and Industrial Strategy and delivered by the British Council. We are also grateful to the publishers, journals, and authors who provided their permission for us to reproduce their figures and tables in this review.Peer reviewedPostprin