Label Identification from Statistical Tabulation (LIST) test and evaluation

There are no author-identified significant results in this report

Tide and Tidal Current Prediction by High Speed Digital Computer

The Tide and Tidal Current Tables of the U. S. Coast and Geodetic Survey for 1966 have been computed and edited by a digital computer, the IBM 7094. Prediction by this method is found to be more economical and expedient than by the tide prediction machine in use since 1910. The shift to digital predictions has been gradual. The first program was prepared in 1956 to predict hourly tide heights only, for use in storm surge research. The greatest advantage to digital prediction at that time was the elimination of the hour or more required to set up a new problem on the tide predicting machine, when highly accurate predictions were needed for many short periods. Later, as more efficient computers became available, this program was expanded to include the computation of highs and lows, editing the data in a form suitable for publication and the complete prediction and editing of the tidal current tables. The existing program, to a large degree, reproduced the same calculations formerly made on the analogue tide predicting machine, and with comparable accuracy. The greater versatility of this system invites experimentation, not feasible with the analogue computer. Thus, it is expected that in the long run the switch to digital calculations will lead to an increase in the accuracy of the predictions for stations having complex tide problems. The program grew through the years, and is not the most efficient that could be prepared today. Nevertheless, it appears doubtful that the improved efficiency would justify a complete revision. This report gives a general description of the program, the input data specifications and samples of the results

Predictor Equations for Beach Processes and Responses

A stepwise (linear) multiple regression procedure is applied to 11 environmental variables (or predictors) in the beach-ocean-atmosphersey stema t Virginia Beach, Virginia, for the following five predictands: mean longshore current velocity, mean bottom slope in the shoaling-wave zone, average mean grain size in the shoaling-wave zone, and beach deposition and beach erosion on the lower foreshore. Predictors consist of variables related to beach geometry, local water properties, local wind conditions, tidal fluctuations, and wave characteristics The resultant equations are tested against a set of independent data and, with one exception, agree reasonably. It is believed that if the data set were increased to include at least one year\u27s continuous measurements the procedure outlined would yield valid equations for all but stormy-weather conditions.It is presupposed that some provision will have to be made for preconditioning the data, as \u27storm\u27 and \u27nonstorm\u27 data will probably have to be analyzed separately

Kemampuan Pemahaman Konseptual dan Algoritmik Siswa dalam Menyelesaikan Soal-Soal Reaksi Redoks

Tujuan penelitian ini untuk mendeskripsikan kemampuan pemahaman konseptual dan algoritmik siswa dalam menyelesaikan soal-soal reaksi redoks. Penelitian ini dilakukan di dua sekolah yang berada di Kabupaten Gorontalo yaitu di SMAN 1 dan SMAN 2 Limboto. Pengambilan sampel dengan menggunakan teknik simple random sampling,sebanyak 80 siswa dengan objek penelitian siswa yang terdistribusi di SMAN 1 Limboto kelas X MIA4 dan X kelas MIA5 sedangkan di SMAN 2 Limboto kelas X MIA1 dan kelas X MIA2. Kemampuan pemahaman konseptual dan algoritmik siswa diukur dengan menggunakan instrumen tes berbentuk essay. Hasil penelitian menunjukan bahwa kemampuan pemahaman konseptual dan algoritmik siswa dalam menyelesaikan soal-soal reaksi redoks termasuk kategori sangat rendah yaitu sebesar 25,25 % (pemahaman konseptual) dan 12,75 % (pemahaman algoritmik)

Generation of uniform chromaticity scale imagery from LANDSAT data

There are no author-identified significant results in this report

A unique small cell lung carcinoma disease progression model shows progressive accumulation of cancer stem cell properties and CD44 as a potential diagnostic marker

OBJECTIVES: Cancer stem cells (CSCs) have been implicated in disease progression of aggressive cancers including small cell lung carcinoma (SCLC). Here, we have examined the possible contribution of CSCs to SCLC progression and aggressiveness. MATERIALS AND METHODS: GLC-14, GLC-16 and GLC-19 SCLC cell lines derived from one patient, representing increasing progressive stages of disease were used. CSC marker expressions was determined by RT-qPCR and western blotting analyses, and heterogeneity was studied by CSC marker expression by immunofluorescence microscopy and flow cytometry. Colony formation assays were used to assess stem cell properties and therapy sensitivity. RESULTS: Increasing expression of stem cell markers MYC, SOX2 and particularly CD44 were found in association with advancing disease. Single and overlapping expression of these markers indicated the presence of different CSC populations. The accumulation of more homogeneous double- and triple-positive CSC populations evolved with disease progression. Functional characterization of CSC properties affirmed higher proficiency of colony forming ability and increased resistance to γ-irradiation in GLC-16 and GLC-19 compared to GLC-14. GLC-19 colony formation was significantly inhibited by a human anti-CD44 antibody. CONCLUSION: The progressive increase of MYC, SOX2 and particularly CD44 expression that was accompanied with enhanced colony forming capacity and resistance in the in vitro GLC disease progression model, supports the potential clinical relevance of CSC populations in malignancy and disease relapse of SCLC

A comparison of magnetic resonance, X-ray and positron emission particle tracking measurements of a single jet of gas entering a bed of particles

Measurements of the lengths of a single jet of gas entering a packed bed were made using magnetic resonance imaging (MRI), positron emission particle tracking (PEPT) and X-ray radiography and the results compared. The experiments were performed using a Perspex bed (50 mm i.d.) of poppy seeds: air at 298 K was admitted to the base of the bed through a single, central orifice, 2 mm in diameter. Poppy seeds (Geldart Group B, measured minimum fluidisation velocity with air at 298 K and 1 atm of 0.13 m/s and particle density ~1060 kg/m3) were used because of their high content of oil, which contains mobile protons and hence is suitable for MRI examination. The lengths of jet measured using the three techniques were in agreement between 50 m/s < Uo < 100 m/s, where Uo is the superficial velocity through the orifice. Below Uo = 50 m/s, X-ray measurements of jet lengths were shorter than those measured using MRI. This was attributed to the minimum diameter of void, found to be 5 mm, detectable in a 50 mm bed using ultra-fast X-ray measurements. PEPT is most commonly used to calculate particle velocities, whilst jet lengths are usually calculated from determinations of voidage. However, the particle locations determined in this work by PEPT were used to calculate a fractional occupancy count, from which a jet length could be inferred.RCUK, OtherThis is the final version of the article. It first appeared from Elsevier via http://dx.doi.org/10.1016/j.ces.2014.09.02

Role of Viral Hemorrhagic Septicemia Virus Matrix (M) Protein in Suppressing Host Transcription

ABSTRACT Viral hemorrhagic septicemia virus (VHSV) is a pathogenic fish rhabdovirus found in discrete locales throughout the Northern Hemisphere. VHSV infection of fish cells leads to upregulation of the host's virus detection response, but the virus quickly suppresses interferon (IFN) production and antiviral gene expression. By systematically screening each of the six VHSV structural and nonstructural genes, we identified matrix protein (M) as the virus' most potent antihost protein. Only M of VHSV genotype IV sublineage b (VHSV-IVb) suppressed mitochondrial antiviral signaling protein (MAVS) and type I IFN-induced gene expression in a dose-dependent manner. M also suppressed the constitutively active simian virus 40 (SV40) promoter and globally decreased cellular RNA levels. Chromatin immunoprecipitation (ChIP) studies illustrated that M inhibited RNA polymerase II (RNAP II) recruitment to gene promoters and decreased RNAP II C-terminal domain (CTD) Ser2 phosphorylation during VHSV infection. However, transcription directed by RNAP I to III was suppressed by M. To identify regions of functional importance, M proteins from a variety of VHSV strains were tested in cell-based transcriptional inhibition assays. M of a particular VHSV-Ia strain, F1, was significantly less potent than IVb M at inhibiting SV40/luciferase (Luc) expression yet differed by just 4 amino acids. Mutation of D62 to alanine alone, or in combination with an E181-to-alanine mutation (D62A E181A), dramatically reduced the ability of IVb M to suppress host transcription. Introducing either M D62A or D62A E181A mutations into VHSV-IVb via reverse genetics resulted in viruses that replicated efficiently but exhibited less cytotoxicity and reduced antitranscriptional activities, implicating M as a primary regulator of cytopathicity and host transcriptional suppression. IMPORTANCE Viruses must suppress host antiviral responses to replicate and spread between hosts. In these studies, we identified the matrix protein of the deadly fish novirhabdovirus VHSV as a critical mediator of host suppression during infection. Our studies indicated that M alone could block cellular gene expression at very low expression levels. We identified several subtle mutations in M that were less potent at suppressing host transcription. When these mutations were engineered back into recombinant viruses, the resulting viruses replicated well but elicited less toxicity in infected cells and activated host innate immune responses more robustly. These data demonstrated that VHSV M plays an important role in mediating both virus-induced cell toxicity and viral replication. Our data suggest that its roles in these two processes can be separated to design effective attenuated viruses for vaccine candidates