Polymorphism of the gene GDF9 in sheep of Prikatun type of Altai Mountains breedand its correlation with indices of meat rate productivity

In recent decades in many countries of the world the development of sheep farming is determined by the efficiency of mutton production. One of the promising areas of selection improvement of sheep breeds of meat and meat & wool productivity is an application of molecular-genetic methods. To obtain high-quality mutton it is advisable to use the Altai Mountains sheep breed, which features by high energy of growth of young animals and their adaptability to all-year-round pasture management. One of the candidate genes responsible for sheep meat productivity is the gene of growth differentiation factor -GDF9 (growth differentia­tion factor 9). The article presents the results of research of polymorphism of the gene GDF9 and its relation with the parameters of meat productivity in young sheep of Altai Mountains breed. It was found that the rams-carriers of AA genotype outperformed their peers of AG and GG genotypes in terms of pre-slaughter weight, carcass weight, meat yield and meat content ratio by 1.62 and 7.01 kg, 1.34 and 3.98 kg (P<0.05), 1.21 and 1.86 abs. percent, 0.16 and 0.39 units (P<0.05) accordingly. The muscle tissue of rams of the desired genotype featured the highest content of protein, fat and, accordingly, energy value in comparison with AG and GG genotypes. The animals of AA genotype had the largest area of a rib eye and diameter of muscle fibers. The superiority over other genotypes achieved 1.55 cm2 and 1.98 microns in average. In addition the higher number of inter-fiber and inter-bundle inclusions of fat and lower content of connective tissue stipulated higher marbling score (MB)-higher by 3.32 points. The obtained data in­dicate that it is promising methods to increase the number of homozygotic animals in term of gene GDF9 by intended selection of parent animals whose genotype contains a desirable element for further improvement of quantitative and qualitative parameters of meat rate productivity in sheep of Altai Mountains breed

Magnetics Hysteresis Properties and Microstructure of High-Energy (Nd,Dy)–Fe–B Magnets with Low Oxygen Content

Abstract: Magnetic properties and microstructure of high-energy (Nd,Dy)–Fe–B magnets with Dy of no more than 1 wt % prepared via a low-oxygen routine are studied. Oxygen content in magnets does not exceed 0.20 wt %. 0.5 wt %–Dy addition reliably stabilizes the coercivity MHc higher than 13 kOe; in this case, the maximum energy density product (BH)max of magnets is 48.5–49.5 MG Oe. High magnetic hysteresis properties are gained via optimization of chemical and phase compositions of magnets, as well as their microstructure. The grain size of the main Nd2Fe14B phase is approximately 3.5 μm; and according to X-ray analysis, the weight fraction of additional Nd-rich phases (NdOx and Nd2O3) does not exceed 2.5%. Scanning electron microscopy study has demonstrated that in triple junctions of Nd2Fe14B grains there are two types of inclusions (В and С) of the NdOx phase, which significantly differ by their chemical composition. С-phase inclusions with low oxygen content (х ≈ 0.03) are enriched in Fe (40–50 wt %); whereas, В-phase with high oxygen content (х ≈ 0.70) contains 3–5 times less Fe. The angular dependences of coercivity of (Nd,Dy)–Fe–B magnets are presented. © 2021, The Author(s).The work is performed in the framework of state assignment of the Ministry of Education and Science of Russia (theme “Magnet,” No. АААА-А18-118020290129-5)

13C NMR spectrum of crystalline [Rh(Acac) (CO)2]: A contribution to the discussion on [Rh(Acac) (CO)2] molecular structure in the solid state

13C MAS NMR spectrum of polycrystalline [Rh(Acac) (CO)2] (1) displays separate signals from all 7 carbon atoms: 2 doublets from CO ligand carbons along with 5 singlets from Acac carbons. GIPAW calculation of 13C shielding tensor values also revealed non-equivalence of all carbon atoms in molecule 1 in the anisotropic medium of the crystal lattice. Apparently, the C2v symmetry of molecule 1 is broken owing to the asymmetry of its contacts to the neighboring molecules. For example, the contacts O⋯HC of two CO ligands of molecule 1 to the CH group of the closest molecule in the adjacent stack are markedly different: the distances OH are 2.72 and 4.38 Å, the distances OC are 3.65 and 5.00 Å, the angles OHC are 164.9° and 126.4°

Temporal and structural genetic variation in reindeer (Rangifer tarandus) associated with the pastoral transition in Northwestern Siberia

Funding Information NordForsk. Grant Number: 76915 ERC Advanced Grant. Grant Numbers: 295458, ESRC ES/, M011054/1 JPI HUMANOR ERC Starting GrantPeer reviewedPublisher PD

Development of a “smart contract” for an affiliate program based on blockchain technology

Цель работы: разработка концепции эффективной системы учета и отслеживания продукции, основанной на применении блокчейн-технологии, в связи c изменениями в маркировке табачной продукции. Задачи работы: - изучить принципы блокчейн технологий; - изучить принципы «smart-контракта» технологии; - рассмотреть преимущества и недостатки технологии; - разработать smart-контракт; - оценить экономическую эффективность проекта. Объект исследования – блокчейн-технологии. Предмет исследования - процесс маркировки продукции. В первой главе приведен обзор теоретического материала по технологии блокчейн. Вторая глава посвящена разработке методики использования «smart-контракта» при маркировке табачной продукции. В третьей главе рассмотрено применение смарт - контракта для конкретного бизнес-процесса. Результаты работы: практическим результатом работы является концепция умного контракта, который существенно упростит процесс, а также позволит маркировать продукцию и контролировать сбыт.The main objective is to develop the concept of effective system accounting and product tracking, based on using of blockchain system, due to changes in the labeling of tobacco products. Tasks of this research: - to examine principles of technology blockchain system - to examine principles of “Smart contract” technology - to consider the advantages and disadvantages of technology -to develop a smart contract - to examine the economical efficient of this project The object of this research is block-technology. The subject of the study is the process of labeling products. The first chapter provides an overview of theoretical material of blockchain technology. The second chapter is devoted to developing a methodology of using a "smart contract" for the labeling of tobacco products. The third chapter deals with the application of a smart contract for a particular business process The result of this Project: the practical result of the work is the concept of a smart contract, which will greatly simplify the process and will also allow to label products and control sales

A PEN INJECTOR FOR SELF-ADMINISTRATION OF FOLLITROPIN ALPHA SOLUTION WITH A MINIMAL DOSE INCREMENT OF 5 IU

To date, self administrations of recombinant human follicle-stimulating hormone (rhFSH) are carried out using pen injectors with minimal dose increments of 8.33 IU or 12.5 IU (IU – international units of activity). To ensure the accuracy of dose titration and to minimize the dose adjustments during the treatment, new pen injectors are needed. Those would provide for the self administration of rhFSH in a wide range of doses and with a dose increment less than the existing fractional values. Aim: to develop a pre-filled, multi-dose pen injector for rhFSH solution with a 5 IU dose increment and a dose setting from 0 to 300 IU.Materials and methods. Dispensed volumes containing the minimal dose of 5 IU were measured gravimetrically.Results. After testing 48 pen injectors the estimated minimal dose of 5 IU (0.0083 ml of the solution) produced the following results: the mean value of the dispensed volume was 0.0081 ml, the mean deviation was ± 0.0006 ml (or ± 0.36 IU). The results are in consistence with the ISO 11608-1:2014(E) requirements of dose accuracy for the minimal dose setting in commercial injectors.Conclusion. We have developed a pen injector for rhFSH administration with a dose setting from 0 to 300 IU and with a minimal increment of dose adjustment of 5 IU. The individual approach to the dose selection/adjustment with a minimal increment of 5 IU may help achieving a highly accurate administration of rhFSH for the controlled ovarian hyperstimulation and for the induced-ovulation protocols with growth activation of 1-2 follicles