TRY plant trait database - enhanced coverage and open access

Plant traits-the morphological, anatomical, physiological, biochemical and phenological characteristics of plants-determine how plants respond to environmental factors, affect other trophic levels, and influence ecosystem properties and their benefits and detriments to people. Plant trait data thus represent the basis for a vast area of research spanning from evolutionary biology, community and functional ecology, to biodiversity conservation, ecosystem and landscape management, restoration, biogeography and earth system modelling. Since its foundation in 2007, the TRY database of plant traits has grown continuously. It now provides unprecedented data coverage under an open access data policy and is the main plant trait database used by the research community worldwide. Increasingly, the TRY database also supports new frontiers of trait-based plant research, including the identification of data gaps and the subsequent mobilization or measurement of new data. To support this development, in this article we evaluate the extent of the trait data compiled in TRY and analyse emerging patterns of data coverage and representativeness. Best species coverage is achieved for categorical traits-almost complete coverage for 'plant growth form'. However, most traits relevant for ecology and vegetation modelling are characterized by continuous intraspecific variation and trait-environmental relationships. These traits have to be measured on individual plants in their respective environment. Despite unprecedented data coverage, we observe a humbling lack of completeness and representativeness of these continuous traits in many aspects. We, therefore, conclude that reducing data gaps and biases in the TRY database remains a key challenge and requires a coordinated approach to data mobilization and trait measurements. This can only be achieved in collaboration with other initiatives

Enhanced protein immobilization on polymers - a plasma surface activation study

Over the last years, polymers have gained great attention as substrate material, because of the possibility to produce low-cost sensors in a high-throughput manner or for rapid prototyping and the wide variety of polymeric materials available with different features (like transparency, flexibility, stretchability, etc.). For almost all biosensing applications, the interaction between biomolecules (for example, antibodies, proteins or enzymes) and the employed substrate surface is highly important. In order to realize an effective biomolecule immobilization on polymers, different surface activation techniques, including chemical and physical methods, exist. Among them, plasma treatment offers an easy, fast and effective activation of the surfaces by micro/nanotexturing and generating functional groups (including carboxylic acids, amines, esters, aldehydes or hydroxyl groups). Hence, here we present a systematic and comprehensive plasma activation study of various polymeric surfaces by optimizing different parameters, including power, time, substrate temperature and gas composition. Thereby, the highest immobilization efficiency along with a homogenous biomolecule distribution is achieved with a 5-min plasma treatment under a gas composition of 50% oxygen and nitrogen, at a power of 1000 W and a substrate temperature of 80 C. These results are also confirmed by different surface characterization methods, including SEM, XPS and contact angle measurements

Early-succession secondary forests following agropastoral abandonment are key winter habitats for the conservation of a priority bird in the European Alps

AbstractIn contrast to old-growth forests, early-successional stands remain understudied despite potentially harbouring species of conservation interest. With this work, focused on hazel grouse Tetrastes bonasia, a cryptic and indicator species known to select for close-to-natural forests, we evaluated winter densities, home range, microhabitat selection and diet, combining DNA-based mark-recapture and metabarcoding from faecal samples. In total, 216 droppings, collected over 2 years along forest transects in the Italian Alps, were successfully genotyped and 43 individuals were identified. Density estimates were similar to values reported by other studies in the Alps with an average of 4.5 and 2.4 individuals/km2 in the first and second study year, respectively, and mean home ranges estimated at 0.95 km2. According to habitat selection models and eDNA-based diet analysis, hazel grouse selected early-succession secondary-growth forests formed after the abandonment of traditional agropastoral activities. These forests, mostly composed of hazel Corylus avellana, Norway spruce Picea abies and Sorbus spp., provided winter food resources and shelter. The diet analysis also highlighted forest arthropods as a non-negligible source of food. Birds avoided areas subject to intensive browsing by ungulates; small forest roads seasonally closed to traffic had positive influence on hazel grouse (i.e. higher abundance of droppings), while roads open to traffic had no effect. Importantly, despite the high coverage of mature forest habitats of Community Interest (53% of our study area), droppings were more abundant in non-listed early-succession secondary forests with similar plant composition. Our results suggest that forest succession after agropastoral abandonment may be beneficial for some forest birds of conservation interest, while acknowledging its negative effects on the previous grassland biodiversity. Graphical abstract</jats:p

Early-succession secondary forests following agropastoral abandonment are key winter habitats for the conservation of a priority bird in the European Alps

In contrast to old-growth forests, early-successional stands remain understudied despite potentially harbouring species of conservation interest. With this work, focused on hazel grouse Tetrastes bonasia, a cryptic and indicator species known to select for close-to-natural forests, we evaluated winter densities, home range, microhabitat selection and diet, combining DNA-based mark-recapture and metabarcoding from faecal samples. In total, 216 droppings, collected over 2 years along forest transects in the Italian Alps, were successfully genotyped and 43 individuals were identified. Density estimates were similar to values reported by other studies in the Alps with an average of 4.5 and 2.4 individuals/km2 in the first and second study year, respectively, and mean home ranges estimated at 0.95 km2. According to habitat selection models and eDNA-based diet analysis, hazel grouse selected early-succession secondary-growth forests formed after the abandonment of traditional agropastoral activities. These forests, mostly composed of hazel Corylus avellana, Norway spruce Picea abies and Sorbus spp., provided winter food resources and shelter. The diet analysis also highlighted forest arthropods as a non-negligible source of food. Birds avoided areas subject to intensive browsing by ungulates; small forest roads seasonally closed to traffic had positive influence on hazel grouse (i.e. higher abundance of droppings), while roads open to traffic had no effect. Importantly, despite the high coverage of mature forest habitats of Community Interest (53% of our study area), droppings were more abundant in non-listed early-succession secondary forests with similar plant composition. Our results suggest that forest succession after agropastoral abandonment may be beneficial for some forest birds of conservation interest, while acknowledging its negative effects on the previous grassland biodiversity. Graphical abstract: [Figure not available: see fulltext.

Distant but similar: Simultaneous drop in the abundance of three independent amphibian communities

Amphibian species are declining worldwide, with a negative trend affecting both rare and widespread species. There is increasing evidence that resources must be allocated not only toward the monitoring of rare and charismatic species; however, the attention toward abundant species has often been minimal. Here, we describe the strong reduction in the numbers of several widespread amphibian species over the last 3 years observed in three independent amphibian monitoring studies conducted in an alpine, floodplain, and urban landscape in Italy, Germany, and Russia, respectively. The decline was particularly strong in juveniles, but adults and egg clutches were also affected. Such declining rates, if prolonged in the future years, will likely pose a serious threat to the populations' ability to recover and might increase extinction risk also in abundant and widespread species. © 2022 The Authors. Conservation Science and Practice published by Wiley Periodicals LLC on behalf of Society for Conservation Biology.European Herpetological SocietyInstitute of Plant and Animal EcologyItalian Ministry of Environment, (PNM‐EU‐2018‐0009926)Paneveggio-Pale di San Martino Nature ParkPaneveggio‐Pale di San Martino Nature ParkSEH, (DP‐615, RA‐485/19)Ural Branch, Russian Academy of Sciences, UB RASFunding text 1: The study in Italy was authorized by the Italian Ministry of Environment (authorization PNM-EU-2018-0009926) and supported by Paneveggio-Pale di San Martino Nature Park. Part of funding was provided by the European Herpetological Society (SEH). The study in Germany has been authorized through a nature conservation exemption from the prohibitions of § 44 para. 1 no. 1, 2 BNatSchG (Federal Nature Conservation Act) and § 4 para. 1 no. 1 BArtSchV (Federal Species Protection Ordinance) by the respective responsible lower nature conservation authorities that allowed catching and handling native amphibian species. Parts of the research in Germany have been paid through the Helmholtz International Fellow Award, grant number DP-615, RA-485/19. The study in Russia was performed within the framework of the state contract number 122021000082-0 with the Institute of Plant and Animal Ecology, Ural Branch of the Russian Academy of Sciences.Funding text 2: The study in Italy was authorized by the Italian Ministry of Environment (authorization PNM‐EU‐2018‐0009926) and supported by Paneveggio‐Pale di San Martino Nature Park. Part of funding was provided by the European Herpetological Society (SEH). The study in Germany has been authorized through a nature conservation exemption from the prohibitions of § 44 para. 1 no. 1, 2 BNatSchG (Federal Nature Conservation Act) and § 4 para. 1 no. 1 BArtSchV (Federal Species Protection Ordinance) by the respective responsible lower nature conservation authorities that allowed catching and handling native amphibian species. Parts of the research in Germany have been paid through the Helmholtz International Fellow Award, grant number DP‐615, RA‐485/19. The study in Russia was performed within the framework of the state contract number 122021000082‐0 with the Institute of Plant and Animal Ecology, Ural Branch of the Russian Academy of Sciences

Coping with unpredictable environments: fine-tune foraging microhabitat use in relation to prey availability in an alpine species

Microhabitat utilisation holds a pivotal role in shaping a species’ ecological dynamics and stands as a crucial concern for effective conservation strategies. Despite its critical importance, microhabitat use has frequently been addressed as static, centering on microhabitat preference. Yet, a dynamic microhabitat use that allows individuals to adjust to fine-scale spatio-temporal prey fluctuations, becomes imperative for species thriving in challenging environments. High-elevation ecosystems, marked by brief growing seasons and distinct abiotic processes like snowmelt, winds, and solar radiation, feature an ephemeral distribution of key resources. To better understand species’ strategies in coping with these rapidly changing environments, we delved into the foraging behaviour of the white-winged snowfinch Montifringilla nivalis, an emblematic high-elevation passerine. Through studying microhabitat preferences during breeding while assessing invertebrate prey availability, we unveiled a highly flexible microhabitat use process. Notably, snowfinches exhibited specific microhabitat preferences, favoring grass and melting snow margins, while also responding to local invertebrate availability. This behaviour was particularly evident in snow-associated microhabitats and less pronounced amid tall grass. Moreover, our investigation underscored snowfinches’ fidelity to foraging sites, with over half located within 10 m of previous spots. This consistent use prevailed in snow-associated microhabitats and high-prey-density zones. These findings provide the first evidence of dynamic microhabitat use in high-elevation ecosystems and offer further insights into the crucial role of microhabitats for climate-sensitive species. They call for multi-faceted conservation strategies that go beyond identifying and protecting optimal thermal buffering areas in the face of global warming to also encompass locations hosting high invertebrate densities