Evaluation of the analytical variability of dipstick protein pads in canine urine

BackgroundThe dipstick is a first-line and inexpensive test that can exclude the presence of proteinuria in dogs. However, no information is available about the analytical variability of canine urine dipstick analysis. ObjectivesThe aim of this study was to assess the analytical variability in 2 dipsticks and the inter-operator variability in dipstick interpretation. MethodsCanine urine supernatants (n = 174) were analyzed with 2 commercially available dipsticks. Two observers evaluated each result blinded to the other observer and to the results of the other dipstick. Intra- and inter-assay variability was assessed in 5 samples (corresponding to the 5 different semi-quantitative results) tested 10 consecutive times over 5 consecutive days. The agreement between observers and between dipsticks was evaluated with Cohen's k test. ResultsIntra-assay repeatability was good (3/10 errors), whereas inter-assay variability was higher (from 1/5 to 4/5 discordant results). The concordance between the operators (k = 0.68 and 0.79 for the 2 dipsticks) and that of the dipsticks (k = 0.66 and 0.74 for the 2 operators) was good. However, 1 observer and 1 dipstick overestimated the results compared with the second observer or dipstick. In any case, discordant results accounted for a single unit of the semi-quantitative scale. ConclusionsAs for any other method, analytic variability may affect the semi-quantitation of urinary proteins when using the dipstick method. Subjective interpretation of the pad and, to a lesser extent, intrinsic staining properties of the pads could affect the results. Further studies are warranted to evaluate the effect of this variability on clinical decisions

Unveiling commodities-financial markets intersections from a bibliometric perspective

The prominence of commodity markets within the domains of empirical finance and energy economics is well established, largely due to oil's importance and its relationship with other commodities and financial markets. In this study, we present a bibliometric examination of 437 journal articles addressing the phenomenon of commodity connectedness, spanning the period from 1994 to 2022. The research methods include a blend of qualitative and quantitative approaches, incorporating bibliometrics and content analysis. Based on the findings of the analysis, four primary research streams have been identified within the literature concerning commodity connectedness, namely (1) commodity interconnectivity, (2) the relationship between traditional commodities, renewable energy, and cryptocurrencies, (3) the relationship between oil and stock markets, and (4) studies utilizing copula methods to examine the interconnectivity between oil and financial markets. We proposed 15 future research questions for further investigation in the domain of commodity connectedness, including topics such as the impact of the post-COVID era and global uncertainties on commodity markets, how commodities can address the issue of climate change, the exponential growth of cryptocurrencies as a new financial asset, and the impact of the ongoing Russia-Ukraine conflict on commodity and financial markets

Preliminary validation study of Paraoxonase-1 in horses

Paraoxonase-1 (PON-1) is an anti-oxidant enzyme associated with high-density lipoproteins in blood. PON-1 is a negative acute-phase protein being its plasmatic activity reduced during inflammation due to consumption by oxidants. Considering the possible clinical usefulness of PON-1 as an early inflammatory marker this is a preliminary validation study in horses. Serum PON-1 activity was measured in 69 clinically healthy animals (31 adult female, 18 geldings, 11 stallions, 9 foals) using an enzymatic method adapted from other species. In order to preliminarily assess the possible utility of PON-1 as a marker of Systemic Inflammatory Response Syndrome (SIRS), blood from 6 sick foals, classified according to a validated SIRS scale, was analyzed. Intra- and inter-assay imprecision were assessed by repeated analysis of pooled samples and evaluation of coefficient of variations (CV). Accuracy was indirectly evaluated through linearity under dilution (LUD) and spiking recovery test (SRT). Results of the different groups of healthy horses were compared to each other with a Friedmann test with Bonferroni correction. The method is precise (inter- and inter-assay CVs <5%) and accurate (LUD and SRT fit the linear model). PON-1 activity was higher in foals and in adult females (mean ± SD: 63.7±15.5 and 60.8±10.1, respectively) than in geldings and adult males (52.5±10.2 and 47.2±7.7, respectively). In 3/6 SIRS foals PON-1 activity was lower than the lowest percentile of distribution of healthy foals. This study demonstrated that the method of measurement of PON-1 activity in horses is precise and accurate and PON-1 may be a marker of SIRS

Assessment of susceptibility to European stone fruit yellows phytoplasma of new plum variety and five rootstock/plum variety combinations

Two separate experiments were carried out to assess the plum susceptibility to infection by European stone fruit yellows phytoplasmas during a five years period. Commercial varieties/cultivars and new selections grafted on Myrabolan 29C were evaluated in at least two plots of four plants each. Visual inspection and PCR/RFLP identification of phytoplasmas detected an increasing phytoplasma presence in both symptomatic and asymptomatic plants. Eight Japanese plum selections showed ESFY symptoms or pathogen presence in the 50% of the plants and nine selections showed ESFY infection in 20% of the plants. Only nine selections showed absence of both symptoms and pathogen. Although the European selections/cultivars were not symptomatic, plants belonging to six of these cultivars were positive for phytoplasma infection. The evaluation of cultivar/rootstock combinations indicate phytoplasma presence from the first year after plantation on. Two of the rootstocks seem to induce a delay in symptoms appearance and cultivar T.C. Sun resulted to be the most susceptible to the disease independently from the rootstock employed. Keywords: Japanese plum, European plum, European stone fruit yellows phytoplasmas, resistance, disease

Greenfield FDI attractiveness index: a machine learning approach

This study aims to propose a comprehensive greenfield foreign direct investment (FDI) attractiveness index using exploratory factor analysis and automated machine learning (AML). We offer offer a robust empirical measurement of location-choice factors identified in the FDI literature through a novel method and provide a tool for assessing the countries' investment potential.publishedVersionPaid open acces

Colorimetric and electrophoretic evaluation of lipoprotein fractions in healthy neonatal calves: Comparison with results from adult cows and from calves with inflammatory conditions

High density lipoproteins (HDLs) are pivotal in innate immunity and decrease in serum during inflammation. Several studies have been done about lipoprotein changes in transition cows but little is known about their changes in newborn calves. The aim of this study is to provide information about HDLs in newborn calves, by defining the possible age-related changes in healthy calves compared with adults and by assessing the possible differences in calves with inflammation. Lipoprotein electrophoretic separation (reported as percentages) and colorimetric measurement of HDL (HDL-C) were performed on healthy cows and calves in order to identify possible differences in the lipoprotein profile due to the age. Then, age-matched calves with inflammatory conditions were also evaluated. Results showed that in calves HDL% and VLDL% were lower (mean values ± SD: 77.6% ± 8.6% and 2.6% ± 2.5%, respectively) and LDL% was higher (19.7% ± 7.4%) than in adults (89.0% ± 3.9%; 5.2 ± 2.1% and 5.8% ± 3.1%, respectively). Sick calves revealed a decrease of both HDL% (mean values ± SD: 61.0% ± 22.1%) and HDL-C (22.8 ± 11.6 mg/dL) and an increase of VLDL% (12.1% ± 13.1%) compared with controls (77.6% ± 8.6%; 41.5 ± 11.2 mg/dL and 2.6% ± 2.5%, respectively). Paraoxonase-1 activity, influenced by inflammation and oxidation, was measured, and it appeared correlated with HDL% and HDL-C in sick calves. In conclusion, this study revealed that HDLs concentration in healthy calves is lower than in adults, and further decreases in calves with inflammation, likely due to oxidation

Comparison of three blood transfusion guidelines applied to 31 feline donors to minimise the risk of transfusion-transmissible infections

Objectives The increased demand for animal blood transfusions creates the need for an adequate number of donors. At the same time, a high level of blood safety must be guaranteed and different guidelines (GLs) deal with this topic. The aim of this study was to evaluate the appropriateness of different GLs in preventing transfusion-transmissible infections (TTI) in Italian feline blood donors. Methods Blood samples were collected from 31 cats enrolled as blood donors by the owners' voluntary choice over a period of approximately 1 year. Possible risk factors for TTI were recorded. Based on Italian, European and American GLs, specific TTI, including haemoplasmas, feline leukaemia virus (FeLV), feline immunodeficiency virus (FIV), Anaplasma phagocytophilum, Ehrlichia species, Bartonella species, Babesia species, Theileria species, Cytauxzoon species, Leishmania donovani sensu lato and feline coronavirus (FCoV) were screened. Rapid antigen and serological tests and biomolecular investigations (PCR) were used. Several PCR protocols for haemoplasma and FeLV DNA were compared. Results The presence of at least one recognised risk factor for TTI was reported in all cats. Results for FIV and FeLV infections were negative using rapid tests, whereas five (16.1%) cats were positive for FCoV antibodies. Four (12.9%) cats were PCR positive for haemoplasma DNA and one (3.2%) for FeLV provirus, the latter being positive only using the most sensitive PCR protocol applied. Other TTI were not detected using PCR. Conclusions and relevance Blood safety increases by combining the recommendations of different GLs. To reduce the risk of TTI, sensitive tests are needed and the choice of the best protocol is a critical step in improving blood safety. The cost and time of the screening procedures may be reduced if appropriate tests are selected. To this end, the GLs should include appropriate recruitment protocols and questionnaire-based risk profiles to identify suitable donors

Feline gut microbiota composition in association with feline coronavirus infection : a pilot study

Feline coronaviruses (FCoV) colonize the intestinal tract, however, due to not fully understood mutations, they can spread systemically and cause feline infectious peritonitis (FIP). Recent studies on human medicine report that gut microbiota is involved in the development of systemic disorders and could influence the immune response to viral diseases. The aim of this study was to provide preliminary data on the fecal microbiota composition in healthy cats compared to FCoV-infected cats, with and without FIP. Cats were equally grouped as healthy FCoV-negative, healthy FCoV-positive or FIP affected (total n\u202f=\u202f15). Fecal sample were evaluated for the microbiota composition. A total of 3,231,916 sequences were analyzed. The samples' alpha-diversity curves did not reach a proper plateau and, for the beta-diversity, the samples seemed not to group perfectly by category, even if the healthy FCoV-positive group showed a hybrid microbial composition between FCoV-negative and FIP groups. Although there were no taxa significantly linked to the different conditions, some peculiar patterns were recognized: Firmicutes was always the most represented phylum, followed by Bacteroidetes and Actinobacteria. In FCoV-positive cats, the Firmicutes and Bacteroidetes were respectively over- and under-represented, compared to the other groups. Among FIP cats, three subjects shared a similar microbiome, one cat showed a different microbial profile and the other one had the lowest number of diverse phyla. Despite the limited number of animals, some differences in the fecal microbiome between the groups were observed, suggesting to further investigate the possible correlation between gut microbiota and FCoV infection in cats

Factors affecting the spread of "Bois Noir" disease in north Italy vineyards

To define control strategies for “Bois Noir” disease (BN) it is necessary to know factors favouring its spreading by the vector Hyalesthes obsoletus Signoret. During 2003-2006 a research was carried out in 18 vineyards of a grape-growing area of North Italy to assess the influence of insecticides, applied on grapevine canopies, and environment surrounding the vineyards on disease spreading. The vector population density was higher outside than in the centre of the vineyards. Insecticides applied to grapevine canopies did not significantly influence the vector population level in the centre of the vineyards. The majority of vineyards showed randomized distribution of symptomatic grapevines. Seven vineyards had an aggregate distribution due to an edge effect from a border side with nettle. The incidence of border sides not contiguous to other grapevine rows on vineyard surface was positively related to higher levels of BN. The incidence of border sides with nettle on vineyard surface was positively correlated to disease incidence in the vineyards with aggregate distribution of symptomatic grapevines. All the data support the importance of surrounding vegetation as source of inoculum of BN phytoplasma. Molecular analyses on ribosomal and tuf genes show that 16 out of the 18 vineyards were affected only by BN: in 13 only tuf-type I was identified, in 2 only tuf-type II, in 1 both tuf-types, and in 2 it was not possible to identify the tuf-type of phytoplasmas detected. In the weeds tested only tuftype II phytoplasmas were identified while H. obsoletus was carrying both phytoplasma tuf-types.

Molecular characterization of ‘Candidatus Phytoplasma mali’ strains in outbreaks of apple proliferation in north eastern Italy, Hungary, and Serbia

During 2005-2008 apple plants of different varieties showing proliferation symptoms were observed in diverse areas of north eastern Italy, Hungary and Serbia. PCR/RFLP analyses showed that all the samples were infected with ‘Candidatus Phytoplasma mali’. In the 16S plus spacer region two phytoplasma profiles (P-I and P-II) were distinguished. P-I profile was detected in reference strains AP, AT1, AT2, in samples from Serbia, and in the majority of samples from Trentino; the P-II profile was prevalent in samples from Veneto; both profiles were identified in samples from Hungary, in some cases together in single samples. The analyses of rpl22-s3 genes allow the identification, in all the samples showing a P-I profile, the presence of phytoplasmas belonging to rpX-A subgroup, while in the samples showing a P-II profile it was possible to distinguish the other three reported rpX subgroups. In the majority of samples from the Veneto region phytoplasmas belonging to rpX-D subgroup were identified, while rpX-B and rpX-C subgroups were identified only in a few samples from Trentino and Veneto regions, respectively. Further RFLP analyses on AP13/AP10 amplicons differentiate among strains belonging to the rpX-A subgroup: the samples from Serbia show AP profiles, while those from Trentino show AT-2 profiles. In the samples from Hungary the presence of AT1, AT2, and AP profiles was identified.Keywords: Apple, ‘Candidatus Phytoplasma mali’, phytoplasma strains, PCR/RFLP analyses, epidemiolog