To define control strategies for “Bois Noir” disease (BN) it is necessary to know factors favouring its spreading by the vector Hyalesthes obsoletus Signoret. During 2003-2006 a research was carried out in 18 vineyards of a grape-growing area of North Italy to assess the influence of insecticides, applied on grapevine canopies, and environment surrounding the vineyards on disease spreading. The vector population density was higher outside than in the centre of the vineyards. Insecticides applied to grapevine canopies did not significantly influence the vector population level in the centre of the vineyards. The majority of vineyards showed randomized distribution of symptomatic grapevines. Seven vineyards had an aggregate distribution due to an edge effect from a border side with nettle. The incidence of border sides not contiguous to other grapevine rows on vineyard surface was positively related to higher levels of BN. The incidence of border sides with nettle on vineyard surface was positively correlated to disease incidence in the vineyards with aggregate distribution of symptomatic grapevines. All the data support the importance of surrounding vegetation as source of inoculum of BN phytoplasma. Molecular analyses on ribosomal and tuf genes show that 16 out of the 18 vineyards were affected only by BN: in 13 only tuf-type I was identified, in 2 only tuf-type II, in 1 both tuf-types, and in 2 it was not possible to identify the tuf-type of phytoplasmas detected. In the weeds tested only tuftype II phytoplasmas were identified while H. obsoletus was carrying both phytoplasma tuf-types.