831 research outputs found

    Leadership Instruments Library (LIL) for Graduate Research

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    Created by Sam Nickels at the request of the School of Strategic Leadership Studies (SSLS) program at James Madison University, 2016-2017. Anyone should feel free to suggest updates, expand the information on instruments or add new instruments. Send the information to SSLS and we’ll screen the info before adding it to the library. Suggested changes/additions need to be well documented with citations. To provide corrected or new information, please contact the School of Strategic Leadership Studies at James Madison University: [email protected]. We hope you find it useful! Dr. Sam Nickels, former graduate assistant at SSLS and Dr. Karen Ford, Head, SSLS

    An Archaeological Survey for the Bexar County Correctional Facility at Cagnon Road, San Antonio, Texas

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    An archaeological survey was conducted near Cagnon Road by the Center for Archaeological Research of The University of Texas for Bexar County. The project area is the proposed location for the Bexar County Correctional Facility. Intensive pedestrian survey and backhoe trenching were employed to determine the presence and depth of cultural material in the project area. One prehistoric site, characterized by a surface lithic scatter, was identified. Due to thin topsoil and agricultural activities, the site exists in a disturbed context. Furthermore, the presence of shallow Cretaceous age subsoil precludes finding buried in situ cultural material in the project area. Since the Bexar County Correctional Facility will not negatively impact intact cultural deposits it is recommended that construction be allowed to proceed

    An Archaeological Survey of 35 Acres Near Eagle Pass, Maverick County, Texas

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    On April 22 through 24, 1998, staff archaeologists from the Center for Archaeological Research (CAR) conducted a 100 percent pedestrian survey with limited shovel and backhoe testing on two parcels near Eagle Pass, Texas (Figure 1). The archaeological investigation was conducted at the request of the Eagle Pass Independent School District as part of a plan to construct two new elementary schools on the parcels (Figures 2 and 3). The purpose of the survey was to identify archaeological sites visible on the surface as well as areas where sites are potentially buried. CAR archaeologists recorded 27 isolated finds on the 20-acre parcel south of the city. On the 15-acre parcel north of the city they discovered and recorded one archaeological site, with an additional nine isolated finds (Figures 2 and 3). The site has been heavily disturbed due to natural and artificial causes; therefore CAR recommended that the planned construction should be allowed to proceed with no further consultation with the State Historical Preservation Office (SHPO) or the Texas Historical Commission (THC)

    Test Excavations at the Culebra Creek Site, 41BX126, Bexar County, Texas

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    Archaeological test excavations were undertaken at 4IBX126 on Culebra Creek to offset the impact from a proposed Texas Department of Transportation (TxDOT) highway improvement project on Loop 1604 in northwest Bexar County. Archaeological investigations were conducted in three field seasons: the first two seasons were conducted by TxDOT archaeologists and the third was directed by personnel from the Center for Archaeological Research (CAR) of The University of Texas at San Antonio. During the three projects, 55 hand-dug units, 29 backhoe trenches, 36 shovel tests, and eight Gradall trenches were excavated. Seventeen features were recorded; 25 radiocarbon assays were conducted; over 59,000 lithic artifacts were recovered and analyzed; 1,655 liters of sediment float samples were processed; 3,337 kg of burned rock were analyzed; and nearly 300 g of fatmal material and 25 archaeomagnetic samples were analyzed. The testing revealed utilization of the site in the Early, Middle, and Late Archaic periods. The analysis of materials and results of all three field efforts are presented in this single volume. Geoarchaeological investigations show that four terraces (TO, Tl, T2, and T3) in the immediate site area accumulated from the Late Pleistocene through the Holocene. Five Stratigraphic Units (I-V) make up these terraces and overlap one another. The T2 terrace is composed of Stratigraphic Units IT, ill, and IV, while the Tl terrace consists mostly of Stratigraphic Units IV and V. Archaeological materials were discovered in situ within the Tl and T3 terraces and primarily within Stratigraphic Units ill and IV. Radiocarbon assays indicate that Stratigraphic Unit IV formed between at least 4000-2000 B.P., Stratigraphic Unit ill accumulated between approximately 11,500-4000 B.P., and Stratigraphic Unit IT was accreting at least 17,500 years ago. Too little evidence exists to determine the full time ranges of sediment accumulation, and whether significant temporal gaps exist between the sedimentation of these geological units. Archaeological excavations focused on three separate areas: A, B, and C. Area A is a new right-of-way east of the existing right-of-way. Excavations in this area defined a Late Archaic Montell component dating to approximately 2700 B.P. These materials include two burned rock features in situ within Unit IV on the scarp of the T2 terrace. This area probably was occupied by a small residential group during the Late Archaic period. Area B is east of Loop 1604 in the existing right-of-way and on the T2 terrace. Area B contains a Middle Archaic Nolan component in the upper portion of Stratigraphic Unit ill, below a Late Archaic burned rock midden with a central subsurface oven in Unit IV. Area C is in the existing right-of-way west of Loop 1604. Excavations in this area investigated the possibility of an intact Early Archaic occupation; however, no evidence of one was found. In Area B, the Nolan component consisted of lithic artifacts scattered among small burned rock features that probably served as hearths. This component is radiocarbon dated to approximately 4600 B.P. The Late Archaic burned rock midden was apparently used between 4000 B.P. and 2000 B.P. Subfeatures within the central oven indicate multiple cooking events. Ethnographic evidence suggests earth ovens contained food wrapped with insulating material over a layer of hot rocks heated by a coal bed. This was capped with dirt to seal the oven. When cooking was complete, the earth cap is removed to reach the food. CAR conducted earth-oven hot-rock experiments which indicated that local limestone could be used once or at the most twice. Local hot-rock cooking should generate a great deal of burned limestone debris. The framework ofthe feature at 41BX126 represents the cap and rock heating-element dumpings from separate cooking events as well as a few small intact burned rock features that served as ovens or hearths. At the base of the midden were a few depressions that may represent borrow pits used to obtain sediment for the central oven cap. Mixing of temporally distinct artifacts from the Nolan and later occupations occurs in and beyond the midden due to sediment excavation and transportation across the site, and redeposition of materials through erosion of materials off the framework

    Effects of increasing the affinity of CarD for RNA polymerase on Mycobacterium tuberculosis growth, rRNA transcription, and virulence

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    CarD is an essential RNA polymerase (RNAP) interacting protein in Mycobacterium tuberculosis that stimulates formation of RNAP-promoter open complexes. CarD plays a complex role in M. tuberculosis growth and virulence that is not fully understood. Therefore, to gain further insight into the role of CarD in M. tuberculosis growth and virulence, we determined the effect of increasing the affinity of CarD for RNAP. Using site-directed mutagenesis guided by crystal structures of CarD bound to RNAP, we identified amino acid substitutions that increase the affinity of CarD for RNAP. Using these substitutions, we show that increasing the affinity of CarD for RNAP increases the stability of the CarD protein in M. tuberculosis. In addition, we show that increasing the affinity of CarD for RNAP increases the growth rate in M. tuberculosis without affecting 16S rRNA levels. We further show that increasing the affinity of CarD for RNAP reduces M. tuberculosis virulence in a mouse model of infection despite the improved growth rate in vitro. Our findings suggest that the CarD-RNAP interaction protects CarD from proteolytic degradation in M. tuberculosis, establish that growth rate and rRNA levels can be uncoupled in M. tuberculosis and demonstrate that the strength of the CarD-RNAP interaction has been finely tuned to optimize virulence. IMPORTANCE Mycobacterium tuberculosis, the causative agent of tuberculosis, remains a major global health problem. In order to develop new strategies to battle this pathogen, we must gain a better understanding of the molecular processes involved in its survival and pathogenesis. We have previously identified CarD as an essential transcriptional regulator in mycobacteria. In this study, we detail the effects of increasing the affinity of CarD for RNAP on transcriptional regulation, CarD protein stability, and virulence. These studies expand our understanding of the global transcription regulator CarD, provide insight into how CarD activity is regulated, and broaden our understanding of prokaryotic transcription

    Archaeological Investigations within the Church Sacristy at Mission San Jose (41BX3), San Antonio, Bexar County, Texas

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    In November 1981, Ford, Powell and Carson Architects contracted with the Center for Archaeological Research (CAR) of the University of Texas at San Antonio (UTSA) to conduct archaeological investigations to determine the possibility of significant subsurface cultural material within the sacristy at Mission San Jose. Four hand excavated units revealed construction materials and architectural details of what may have been the first church on the grounds of the mission and was later converted to a sacristy for the present church, as well as fragmented human remains
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