540 research outputs found
Convenient broad-host-range unstable vectors for studying stabilization cassettes in diverse bacteria
Background: Low-copy-number vectors of potential wide application in biotechnology need to encode stabilization
modules ensuring their stable inheritance. The efficiency of stabilization may vary depending on the plasmid host so a
thorough analysis of stabilization functions is required before use.
Results: To facilitate such analysis highly unstable, mobilizable, broad-host-range (BHR) vectors based on RK2 replicon
were constructed. The vectors are suitable for testing of various stabilization functions, including plasmid and
chromosomal partitioning cassettes encoding ParB homologues capable of spreading on DNA. The xylE or lacZ
reporter systems facilitate easy monitoring of plasmid segregation.
Conclusion: The range of BHR vectors with different reporter cassettes and alternative mobilization systems expands
their application in diverse bacterial species
Qualitative and quantitative methods in the study of martial arts and combat sports: a panel based on personal experiences
p. 79-83In this panel, five experts present their personal experiences in martial arts and combat sports (MA&CS) research. These experiences represent the two classic approaches to our studies: the quantitative and the qualitative. Above all, the panel aims to foster debate and reflection on the complementarity of these methods when trying to embrace the complexity of MA&CS.S
Corrigendum: Computational Methods for Resting-State EEG of Patients With Disorders of Consciousness
An author name was incorrectly spelled as \u201cUrszulaMarkowska-Kacznar.\u201d The correct spelling is \u201cUrszulaMarkowska-Kaczmar.\u201d The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated
Maintenance therapy of patients with recurrent epithelial ovarian carcinoma with the anti-tumor-associated-mucin-1 antibody gatipotuzumab: results from a double-blind, placebo-controlled, randomized, phase II study
BACKGROUND: Gatipotuzumab is a humanized monoclonal antibody recognizing the carbohydrate-induced epitope of the tumor-associated mucin-1 (TA-MUC1). This study aimed to evaluate the efficacy and safety of switch maintenance therapy with gatipotuzumab in patients with TA-MUC1-positive recurrent ovarian, fallopian tube, or primary high-grade serous peritoneal cancer. PATIENTS AND METHODS: In this double-blind, randomized, placebo-controlled, phase II trial, patients with at least stable disease (SD) following chemotherapy were randomized 2:1 to receive intravenous gatipotuzumab (500 mg followed by 1700 mg 1 week later) or placebo every 3 weeks until tumor progression or unacceptable toxicity occurred. Stratification factors were the number of prior chemotherapy lines (2 versus 3-5), response versus SD after the most recent chemotherapy, and progression-free survival (PFS) <6 versus 6-12 months following the prior therapy. Primary endpoint was PFS according to modified immune-related RECIST 1.1 response criteria. Secondary endpoints were PFS at 6 months, safety, overall response rate, CA-125 progression, overall survival, quality of life, and pharmacokinetics. RESULTS: Overall, 216 patients were randomized to gatipotuzumab (n  =  151) or placebo (n  =  65). Median PFS with gatipotuzumab was 3.5 months as compared with 3.5 months with placebo (hazard ratio 0.96, 95% confidence interval 0.69-1.33, P  =  0.80). No advantage for gatipotuzumab over placebo was seen in the secondary efficacy endpoints or in any stratified subgroups. Gatipotuzumab was well tolerated, with mild to moderate infusion-related reactions being the most common adverse events. CONCLUSIONS: Gatipotuzumab switch maintenance therapy does not improve outcome in TA-MUC1-positive ovarian cancer patients. TRIAL REGISTRATION: ClinicalTrials.govNCT01899599; https://clinicaltrials.gov/ct2/show/NCT01899599
Variability of CO2, CH4, and O2 concentration in the vicinity of a closed mining shaft in the light of extreme weather events—numerical simulations
This is the final version. Available from MDPI via the DOI in this record. Data Availability Statement:
Data are contained within the article.With climate change, more intense weather phenomena can be expected, including pressure drops related to the arrival of an atmospheric front. Such drops of pressure are the main reason for gas emissions from closed mines to the surface, and a closed, empty mine shaft is the most likely route of this emission. Among the gases emitted, the most important are carbon dioxide and methane, creating a twofold problem—greenhouse gas emissions and gas hazards. The work presented in this paper simulated the spread of the mentioned gases near such an abandoned shaft for four variants: model validation, the most dangerous situations found during measurements with or without wind, and a forecast variant for a possible future pressure drop. It was found that a momentary CO2 emission of 0.69 m3/s and a momentary CH4 emission of 0.29 m3/s are possible, which for one hour of the appropriate drop would give hypothetically 2484 m3 CO2 and 1044 m3 CH4. In terms of gas hazards, the area that should be monitored and protected may exceed 25 m from a closed shaft in the absence of wind influence. The wind spreads the emitted gases to distances exceeding 50 m but dilutes them significantly.Research Fund for Coal and Steel
Children’s right to participate: The Lundy model applied to early childhood education and care
Children have the right to express their views in all matters affecting them, and to have
them considered and given due weight. Children’s participation is most meaningful
when rooted in children’s everyday lives, and its promotion should be encouraged from
the youngest ages, especially in early childhood education and care (ecec). In this
paper we apply the Lundy model of participation, widely used in policy, practice and
professional development initiatives, to the ecec context. Based on examples provided
by ecec professionals from Belgium, Greece, Poland, and Portugal, we illustrate the
implementation of the elements of space, voice, audience and influence, proposed
by the Lundy model. We also discuss the interrelations among these elements and the
need for organisational and contextual support to enhance children’s participation.
This paper adds to existing literature, highlighting theoretical and practical issues
associated with the promotion of children’s right to participate in ecec.info:eu-repo/semantics/acceptedVersio
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