Effect of a physical education-based dynamic stretching program on hamstring extensibility in female high-school students

The purpose of the present study was to examine the effects of a dynamic stretching development program followed by a four-week detraining period and maintenance program on hamstring extensibility in a physical education setting. A sample of 108 female high-school students aged 16-17 years from four classes were clustered randomly and assigned to either an experimental or a control group. During physical education sessions, the experimental group students performed a dynamic stretching program twice a week for eight weeks. Subsequently, after a four-week period of detraining, the experimental group students completed a maintenance program twice a week during four weeks. The results of the two-way analysis of variance showed that the physical education-based development program significantly improved students’ hamstring extensibility (p.05), the gains obtained previously were recovered after a four-week maintenance program (p<.001). Hence, a physical education-based dynamic stretching intervention is effective in improving and maintaining hamstring extensibility among female high-school students. However, after four weeks of detraining, students’ flexibility reverts to its baseline levels. These findings could help and guide teachers to design programs that guarantee a feasible and an effective development of flexibility in a physical education setting

¿Cómo cambian los niveles de extensibilidad isquiosural de los estudiantes durante un año académico? Un estudio longitudinal

The main purpose of the present study was to examine the students’ hamstring extensibility levels through an academic year. A sample of 128 11th-grade students from a high school center was assessed by the classic sit-and-reach test in pre-, mid- and post-academic year. The results showed that students’ hamstring extensibility level statistically significantly decreased from pre-academic year (22.1 ± 8.5 cm) to mid- (19.9 ± 8.6 cm; d = -0.26) and post-academic year (18.6 ± 9.3 cm; d = -0.41) (p < 0.001) and from mid-academic year to post-academic year (d = -0.16) (p < 0.001). The results of the McNemar’s test indicated that there was a statistically significant decrease on the proportion of students with normal hamstring extensibility level from pre-academic year to post-academic year (p < 0.05). In conclusion, high-school students lost hamstring extensibility levels during an academic year. As a result of this decrease, the number of students with hamstring extensibility shortening increased by 10.9%. Physical education teachers should implement stretching programs to avoid students’ hamstring extensibility shortening.El objetivo principal del presente estudio fue examinar los niveles de extensibilidad isquiosural de los estudiantes durante un curso académico. Una muestra de 128 estudiantes de 1º de bachillerato de un centro de educación secundaria se evaluó mediante la prueba de classic sit-and-reach al comienzo, mediados y final del curso académico. Los resultados mostraron que el nivel de extensibilidad de isquiosural de los estudiantes disminuyó estadísticamente significativamente desde el comienzo (22,1 ± 8,5 cm) a mediados (19,9 ± 8,6 cm, d = -0,26) y final del año académico (18,6 ± 9,3 cm; d = -0,41) (p < 0,001), y desde mediados al final (d = -0.16) (p < 0,001). Los resultados de la prueba de McNemar indicaron que hubo una disminución estadísticamente significativa en la proporción de estudiantes con un nivel de extensibilidad isquiosural normal desde el comienzo al final del curso académico (p < 0,05). En conclusión, los estudiantes de educación secundaria perdieron niveles de extensibilidad isquiosural durante un curso académico. Como resultado de esta disminución, el número de estudiantes con acortamiento de los músculos isquiosurales aumentó un 10,9%. Los profesores de educación física deberían implementar programas de estiramiento para prevenir el acortamiento de extensibilidad isquiosural de los estudiantes.Facultad de Humanidades y Ciencias de la Educació

Functional impairment of human resident cardiac stem cells by the cardiotoxic antineoplastic agent trastuzumab

Trastuzumab (TZM), a monoclonal antibody against the ERBB2 protein, increases survival in ERBB2-positive breast cancer patients. Its clinical use, however, is limited by cardiotoxicity. We sought to evaluate whether TZM cardiotoxicity involves inhibition of human adult cardiac-derived stem cells, in addition to previously reported direct adverse effects on cardiomyocytes. To test this idea, we exposed human cardiosphere-derived cells (hCDCs), a natural mixture of cardiac stem cells and supporting cells that has been shown to exert potent regenerative effects, to TZM and tested the effects in vitro and in vivo. We found that ERBB2 mRNA and protein are expressed in hCDCs at levels comparable to those in human myocardium. Although clinically relevant concentrations of TZM had no effect on proliferation, apoptosis, or size of the c-kit-positive hCDC subpopulation, in vitro assays demonstrated diminished potential for cardiogenic differentiation and impaired ability to form microvascular networks in TZM-treated cells. The functional benefit of hCDCs injected into the border zone of acutely infarcted mouse hearts was abrogated by TZM: infarcted animals treated with TZM + hCDCs had a lower ejection fraction, thinner infarct scar, and reduced capillary density in the infarct border zone compared with animals that received hCDCs alone (n = 12 per group). Collectively, these results indicate that TZM inhibits the cardiomyogenic and angiogenic capacities of hCDCs in vitro and abrogates the morphological and functional benefits of hCDC transplantation in vivo. Thus, TZM impairs the function of human resident cardiac stem cells, potentially contributing to TZM cardiotoxicity

Reduction of myocardial infarction by postischemic administration of the calpain inhibitor A-705253 in comparison to the Na(+)/H(+) exchange inhibitor Cariporide (R) in isolated perfused rabbit hearts

The calpain inhibitor A-705253 and the Na(+)/H(+) exchange inhibitor Cariporide (R) were studied in isolated perfused rabbit hearts subjected to 60 min occlusion of the ramus interventricularis of the left coronary artery (below the origin of the first diagonal branch), followed by 120 min of reperfusion. The inhibitors were added to the perfusion fluid solely or in combination at the beginning of reperfusion. Hemodynamic monitoring and biochemical analysis of perfusion fluid from the coronary outflow were performed. Myocardial infarct size and area at risk (transiently not perfused myocardium) were determined from left ventricular slices after a special staining procedure with Evans blue and 2,3,5-triphenyltetrazolium chloride. The infarcted area (dead myocardium) was 72.7 +/- 4.0% of the area at risk in untreated controls, but was significantly smaller in the presence of the inhibitors. The largest effect was observed with 10(-6) M A-705253, which reduced the infarcted area to 49.2 +/- 4.1% of the area at risk, corresponding to a reduction of 33.6%. Cariporide (R) at 10(-6) M reduced the infarct size to the same extent. The combination of both inhibitors, however, did not further improve cardioprotection. No significant difference was observed between the experimental groups in coronary perfusion, left ventricular pressure, heart rate, or in the release of lactate dehydrogenase and creatine kinase from heart muscle

¿Cómo cambian los niveles de extensibilidad isquiosural de los estudiantes durante un año académico? Un estudio longitudinal

The main purpose of the present study was to examine the students’ hamstring extensibility levels through an academic year. A sample of 128 11th-grade students from a high school center was assessed by the classic sit-and-reach test in pre-, mid- and post-academic year. The results showed that students’ hamstring extensibility level statistically significantly decreased from pre-academic year (22.1 ± 8.5 cm) to mid- (19.9 ± 8.6 cm; d = -0.26) and post-academic year (18.6 ± 9.3 cm; d = -0.41) (p < 0.001) and from mid-academic year to post-academic year (d = -0.16) (p < 0.001). The results of the McNemar’s test indicated that there was a statistically significant decrease on the proportion of students with normal hamstring extensibility level from pre-academic year to post-academic year (p < 0.05). In conclusion, high-school students lost hamstring extensibility levels during an academic year. As a result of this decrease, the number of students with hamstring extensibility shortening increased by 10.9%. Physical education teachers should implement stretching programs to avoid students’ hamstring extensibility shortening.El objetivo principal del presente estudio fue examinar los niveles de extensibilidad isquiosural de los estudiantes durante un curso académico. Una muestra de 128 estudiantes de 1º de bachillerato de un centro de educación secundaria se evaluó mediante la prueba de classic sit-and-reach al comienzo, mediados y final del curso académico. Los resultados mostraron que el nivel de extensibilidad de isquiosural de los estudiantes disminuyó estadísticamente significativamente desde el comienzo (22,1 ± 8,5 cm) a mediados (19,9 ± 8,6 cm, d = -0,26) y final del año académico (18,6 ± 9,3 cm; d = -0,41) (p < 0,001), y desde mediados al final (d = -0.16) (p < 0,001). Los resultados de la prueba de McNemar indicaron que hubo una disminución estadísticamente significativa en la proporción de estudiantes con un nivel de extensibilidad isquiosural normal desde el comienzo al final del curso académico (p < 0,05). En conclusión, los estudiantes de educación secundaria perdieron niveles de extensibilidad isquiosural durante un curso académico. Como resultado de esta disminución, el número de estudiantes con acortamiento de los músculos isquiosurales aumentó un 10,9%. Los profesores de educación física deberían implementar programas de estiramiento para prevenir el acortamiento de extensibilidad isquiosural de los estudiantes.Facultad de Humanidades y Ciencias de la Educació

Increased Expression of Bcl11b Leads to Chemoresistance Accompanied by G1 Accumulation

BACKGROUND: The expression of BCL11B was reported in T-cells, neurons and keratinocytes. Aberrations of BCL11B locus leading to abnormal gene transcription were identified in human hematological disorders and corresponding animal models. Recently, the elevated levels of Bcl11b protein have been described in a subset of squameous cell carcinoma cases. Despite the rapidly accumulating knowledge concerning Bcl11b biology, the contribution of this protein to normal or transformed cell homeostasis remains open. METHODOLOGY/PRINCIPAL FINDINGS: Here, by employing an overexpression strategy we revealed formerly unidentified features of Bcl11b. Two different T-cell lines were forced to express BCL11B at levels similar to those observed in primary T-cell leukemias. This resulted in markedly increased resistance to radiomimetic drugs while no influence on death-receptor apoptotic pathway was observed. Apoptosis resistance triggered by BCL11B overexpression was accompanied by a cell cycle delay caused by accumulation of cells at G1. This cell cycle restriction was associated with upregulation of CDKN1C (p57) and CDKN2C (p18) cyclin dependent kinase inhibitors. Moreover, p27 and p130 proteins accumulated and the SKP2 gene encoding a protein of the ubiquitin-binding complex responsible for their degradation was repressed. Furthermore, the expression of the MYCN oncogene was silenced which resulted in significant depletion of the protein in cells expressing high BCL11B levels. Both cell cycle restriction and resistance to DNA-damage-induced apoptosis coincided and required the histone deacetylase binding N-terminal domain of Bcl11b. The sensitivity to genotoxic stress could be restored by the histone deacetylase inhibitor trichostatine A. CONCLUSIONS: The data presented here suggest a potential role of BCL11B in tumor survival and encourage developing Bcl11b-inhibitory approaches as a potential tool to specifically target chemoresistant tumor cells