138 research outputs found

    Effect of Water Content on the Thermal Inactivation Kinetics of Horseradish Peroxidase Freeze-Dried from Alkaline pH

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    The thermal inactivation of horseradish peroxidase freeze-dried from solutions of different pH (8, 10 and 11.5, measured at 25 C) and equilibrated to different water contents was studied in the temperature range from 110 to 150 C. The water contents studied (0.0, 1.4, 16.2 and 25.6 g water per 100 g of dry enzyme) corresponded to water activities of 0.0, 0.11, 0.76 and 0.88 at 4 C. The kinetics were well described by a double exponential model. The enzyme was generally more stable the lower the pH of the original solution, and for all pH values, the maximum stability was obtained at 1.4 g water/100 g dry enzyme. Values of z were generally independent of water content and of the pH of the original solution, and in the range of 15–25 Β°C, usually found in neutral conditions, with the exception of the enzyme freeze dried from pH 11.5 and equilibrated with phosphorus pentoxide, where a z-value of the stable fraction close to 10 C was found

    Π€ΠΎΡ€ΠΌΠΈΡ€ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΈ ΠΏΡ€ΠΎΠ΄Π²ΠΈΠΆΠ΅Π½ΠΈΠ΅ ΠΊΡ€Π΅Π΄ΠΈΡ‚Π½Ρ‹Ρ… ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ‚ΠΎΠ² ΠΈ услуг

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    ΠšΡ€Π΅Π΄ΠΈΡ‚Π½Π°Ρ услуга - ΠΎΠ΄Π½Π° ΠΈΠ· ΡΠΎΡΡ‚Π°Π²Π»ΡΡŽΡ‰ΠΈΡ… ΠΊΡ€Π΅Π΄ΠΈΡ‚Π½ΠΎΠΉ ΠΏΠΎΠ»ΠΈΡ‚ΠΈΠΊΠΈ Π±Π°Π½ΠΊΠ°. Π‘Ρ‚Ρ€Π΅ΠΌΠ»Π΅Π½ΠΈΠ΅ ΠΊ ΠΌΠ°ΠΊΡΠΈΠΌΠ°Π»ΡŒΠ½ΠΎΠΌΡƒ ΠΏΠΎΠ»ΡƒΡ‡Π΅Π½ΠΈΡŽ ΠΏΡ€ΠΈΠ±Ρ‹Π»ΠΈ ΠΎΡ‚ Π΅Π΅ прСдоставлСния ΠΈ ΡƒΠ²Π΅Π»ΠΈΡ‡Π΅Π½ΠΈΡŽ ΠΏΠΎΡ‚ΠΎΠΊΠ° ΠΊΠ»ΠΈΠ΅Π½Ρ‚ΠΎΠ² ΠΏΡ€ΠΈΠ²ΠΎΠ΄ΠΈΡ‚ ΠΊ созданию комплСкса (совокупности) услуг, Π° ΠΈΠΌΠ΅Π½Π½ΠΎ - ΠΊΡ€Π΅Π΄ΠΈΡ‚Π½ΠΎΠΌΡƒ ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ‚Ρƒ. Π˜Π·ΡƒΡ‡ΠΈΠ² интСрСсы Ρ…ΠΎΠ·ΡΠΉΡΡ‚Π²ΡƒΡŽΡ‰ΠΈΡ… ΡΡƒΠ±ΡŠΠ΅ΠΊΡ‚ΠΎΠ², ΠΈΡ… потрСбности, Π±Π°Π½ΠΊ ΠΌΠΎΠΆΠ΅Ρ‚ Π±ΠΎΠ»Π΅Π΅ Ρ‚ΠΎΡ‡Π½ΠΎ Ρ€Π°Π·Ρ€Π°Π±Π°Ρ‚Ρ‹Π²Π°Ρ‚ΡŒ ΠΏΡ€ΠΈΠΎΡ€ΠΈΡ‚Π΅Ρ‚Ρ‹ ΠΊΡ€Π΅Π΄ΠΈΡ‚Π½ΠΎΠΉ ΠΏΠΎΠ»ΠΈΡ‚ΠΈΠΊΠΈ Π²ΠΎ Π²Π·Π°ΠΈΠΌΠΎΠΎΡ‚Π½ΠΎΡˆΠ΅Π½ΠΈΡΡ… с Ρ€Π΅Π°Π»ΡŒΠ½Ρ‹ΠΌ сСктором экономики. ΠžΠ±Ρ€Π°Ρ‚Π½Π°Ρ Π·Π°Π²ΠΈΡΠΈΠΌΠΎΡΡ‚ΡŒ этого Ρ„Π°ΠΊΡ‚ΠΎΡ€Π° - созданиС ΠΊΠΎΠ½ΠΊΡ€Π΅Ρ‚Π½ΠΎΠ³ΠΎ ΠΊΡ€Π΅Π΄ΠΈΡ‚Π½ΠΎΠ³ΠΎ ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ‚Π° ΠΈ построСниС систСмы продвиТСния ΠΊΡ€Π΅Π΄ΠΈΡ‚Π½Ρ‹Ρ… услуг, которая ΠΏΡ€ΠΈΠ·Π²Π°Π½Π° ΠΎΡΡƒΡ‰Π΅ΡΡ‚Π²Π»ΡΡ‚ΡŒ Π°Π½Π°Π»ΠΈΠ·, диагностику Ρ‚Π΅ΠΊΡƒΡ‰Π΅ΠΉ ситуации, обСспСчСниС взаимодСйствия ΠΌΠ΅ΠΆΠ΄Ρƒ Π±Π°Π½ΠΊΠ°ΠΌΠΈ, Π½Π°Ρ†Π΅Π»ΠΈΡ‚ΡŒ Π²Π·Π°ΠΈΠΌΠΎΠΎΡ‚Π½ΠΎΡˆΠ΅Π½ΠΈΡ Π±Π°Π½ΠΊΠ° ΠΈ Π·Π°Π΅ΠΌΡ‰ΠΈΠΊΠ° Π½Π° Π΄ΠΎΠ»Π³ΠΎΡΡ€ΠΎΡ‡Π½ΡƒΡŽ пСрспСктиву

    Evaluating the potential of high pressure high temperature and thermal processing on volatile compounds, nutritional and structural properties of orange and yellow carrots

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    The present study compares the impact of thermal and high pressure high temperature(HPHT) processing on volatile profile (via a non-targeted headspace fingerprinting) and structural and nutritional quality parameter (via targeted approaches) of orange and yellow carrot purees. The effect of oil enrichment was also considered. Since oil enrichment affects compounds volatility, the effect of oil was not studied when comparing the volatile fraction. For the targeted part, as yellow carrot purees were shown to contain a very low amount of carotenoids, focus was given to orange carrot purees. The results of the non-targeted approach demonstrated HPHT processing exerts a distinct effect on the volatile fractions compared to thermal processing. In addition, different colored carrot varieties are characterized by distinct headspace fingerprints. From a structural point of view, limited or no difference could be observed between orange carrot purees treated with HPHT or HT processes, both for samples without and with oil. From nutritional point of view, only in samples with oil, significant isomerisation of all-trans-Ξ²-carotene occurred due to both processing. Overall, for this type of product and for the selected conditions, HPHT processing seems to have a different impact on the volatile profile but rather similar impact on the structural and nutritional attributes compared to thermal processing

    Qualitative Descriptive Research Investigating Burn Survivors’ Perspectives on Quality of Care Aspects

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    Burn care quality indicators are used to monitor and improve quality of care and for benchmark purposes. The perspectives of burn survivors, however, are not included in current sets of quality indicators while patient-centred care gains importance. The aim of this study was to explore burn survivors’ perspectives on quality aspects of burn care, which was used to translate their perspectives into patient-centred quality of care indicators. Qualitative descriptive research was conducted in a patient panel group. First, thematic analysis was applied to the focus groups to identify overarching themes. Second, patient-centred quality indicators, informed by burn survivors’ valued aspects of care, were defined. Ten burn survivors with an average age of 54 years (SD = 11; range 38–72 years) and mean TBSA burned of 14% (SD = 11%; range 5–35%) participated in two focus groups. Four overarching themes were identified, pointing to the importance of (1) information tailored to the different phases of recovery, (2) significant others’ wellbeing and involvement, (3) a therapeutic relationship and low-threshold access to healthcare professionals and (4) to participate in decision-making. Eighteen patient-centred process quality of care indicators within nine aspects of care were formulated. The overarching themes are reflected in patient-centred quality indicators, which present a broadened and complementary view of existing clinical quality indicators for burn care. Evaluating these patient-centred quality indicators may increase quality of care and refine patient-centred care.</p

    Rasch analysis of the Patient and Observer Scar Assessment Scale (POSAS) in burn scars

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    The Patient and Observer Scar Assessment Scale (POSAS) is a questionnaire that was developed to assess scar quality. It consists of two separate six-item scales (Observer Scale and Patient Scale), both of which are scored on a 10-point rating scale. After many years of experience with this scale in burn scar assessment, it is appropriate to examine its psychometric properties using Rasch analysis. Cross-sectional data collection from seven clinical trials resulted in a data set of 1,629 observer scores and 1,427 patient scores of burn scars. We examined the person-item map, item fit statistics, reliability, response category ordering, and dimensionality of the POSAS. The POSAS showed an adequate fit to the Rasch model, except for the item surface area. Person reliability of the Observer Scale and Patient Scale was 0.82 and 0.77, respectively. Dimensionality analysis revealed that the unexplained variance by the first contrast of both scales was 1.7 units. Spearman correlation between the Observer Scale Rasch measure and the overall opinion of the clinician was 0.75. The Rasch model demonstrated that the POSAS is a reliable and valid scale that measures the single-construct scar qualit

    Demonstration of Ignition Radiation Temperatures in Indirect-Drive Inertial Confinement Fusion Hohlraums

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    Lawson Criterion for Ignition Exceeded in an Inertial Fusion Experiment

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