Glycan Structures Contain Information for the Spatial Arrangement of Glycoproteins in the Plasma Membrane

Glycoconjugates at the cell surface are crucial for cells to communicate with each other and the extracellular microenvironment. While it is generally accepted that glycans are vectorial biopolymers, their information content is unclear. This report provides evidence that distinct N-glycan structures influence the spatial arrangement of two integral membrane glycoproteins, Kv3.1 and E-cadherin, at the adherent membrane which in turn alter cellular properties. Distinct N-glycan structures were generated by heterologous expression of these glycoproteins in parental and glycosylation mutant Chinese hamster ovary cell lines. Unlike the N-linked glycans, the O-linked glycans of the mutant cell lines are similar to those of the parental cell line. Western and lectin blots of total membranes and GFP immunopurified samples, combined with glycosidase digestion reactions, were employed to verify the glycoproteins had predominantly complex, oligomannose, and bisecting type N-glycans from Pro(-)5, Lec1, and Lec10B cell lines, respectively. Based on total internal reflection fluorescence and differential interference contrast microscopy techniques, and cellular assays of live parental and glycosylation mutant CHO cells, we propose that glycoproteins with complex, oligomannose or bisecting type N-glycans relay information for localization of glycoproteins to various regions of the plasma membrane in both a glycan-specific and protein-specific manner, and furthermore cell-cell interactions are required for deciphering much of this information. These distinct spatial arrangements also impact cell adhesion and migration. Our findings provide direct evidence that N-glycan structures of glycoproteins contribute significantly to the information content of cells

Integrated precipitable water from GPS observations and CIMEL sunphotometer measurements at CGO Belsk

This paper describes results of integrated precipitable water co-located measurements from two techniques: GPS solution and CIMEL-318 sun-photometer. Integrated Precipitable Water (IPW) is an important meteorological parameter and is derived from GPS tropospheric solutions for GPS station at Central Geophysical Observatory (CGO), Polish Academy of Sciences (PAS), Belsk and compared with sunphotometer (CIMEL-318 device by Cimel Electronique) data provided by Aerosol Robotic Network (AERONET). Two dedicated and independent GPS solutions: network solution in the sub-network of European Permanent Network (EPN) and precise point positioning solution have been made to obtain tropospheric delays. The quality of dedicated tropospheric solutions has been verified by comparison with EPN tropospheric combined product. Several IPW comparisons and analyses revealed systematic difference between techniques (difference RMS is over 1 mm). IPW bias changes with season: annual close to 1 mm IPW (and semi-annual term also present). IPW bias is a function of atmospheric temperature. Probable cause of this systematic deficiency in solar photometry as IPW retrieval technique is a change of optical filter characteristics in CIMEL

Analysis of CEGRN 2005 as the eighth of CERGOP observing campaigns

Strategy of analysis and results from solution of CEGRN epoch campaign in 2005 Combined solution of CEGRN 2005 based or individual solutions from six an: centres and its comparison with CEGRN 2003 coordinates. Time evolution of coordinates at some long-term observed CEGRN sites obtained during epoch camp since 1994 and the related problems

Geodetic and geodynamic studies at Department of Geodesy and Geodetic Astronomy WUT

The article presents current issues and research work conducted in the Department of Geodesy and Geodetic Astronomy at the Faculty of Geodesy and Cartography at Warsaw University of Technology. It contains the most important directions of research in the fields of physical geodesy, satellite measurement techniques, GNSS meteorology, geodynamic studies, electronic measurement techniques and terrain information systems