Full-Stokes polarimetry with circularly polarized feeds - Sources with stable linear and circular polarization in the GHz regime

We present a pipeline that allows recovering reliable information for all four Stokes parameters with high accuracy. Its novelty relies on the treatment of the instrumental effects already prior to the computation of the Stokes parameters contrary to conventional methods, such as the M\"uller matrix one. The instrumental linear polarization is corrected across the whole telescope beam and significant Stokes $Q$ and $U$ can be recovered even when the recorded signals are severely corrupted. The accuracy we reach in terms of polarization degree is of the order of 0.1-0.2 %. The polarization angles are determined with an accuracy of almost 1$^{\circ}$. The presented methodology was applied to recover the linear and circular polarization of around 150 Active Galactic Nuclei. The sources were monitored from July 2010 to April 2016 with the Effelsberg 100-m telescope at 4.85 GHz and 8.35 GHz with a cadence of around 1.2 months. The polarized emission of the Moon was used to calibrate the polarization angle. Our analysis showed a small system-induced rotation of about 1$^{\circ}$ at both observing frequencies. Finally, we identify five sources with significant and stable linear polarization; three sources remain constantly linearly unpolarized over the period we examined; a total of 11 sources have stable circular polarization degree $m_\mathrm{c}$ and four of them with non-zero $m_\mathrm{c}$. We also identify eight sources that maintain a stable polarization angle over the examined period. All this is provided to the community for polarization observations reference. We finally show that our analysis method is conceptually different from the traditionally used ones and performs better than the M\"uller matrix method. Although it was developed for a system equipped with circularly polarized feeds it can easily be modified for systems with linearly polarized feeds as well.Comment: 19 pages, 17 figures, accepted for publication in Astronomy & Astrophysics on May 30, 201

Novel curcumin- and emodin-related compounds identified by in silico 2D/3D conformer screening induce apoptosis in tumor cells

BACKGROUND: Inhibition of the COP9 signalosome (CSN) associated kinases CK2 and PKD by curcumin causes stabilization of the tumor suppressor p53. It has been shown that curcumin induces tumor cell death and apoptosis. Curcumin and emodin block the CSN-directed c-Jun signaling pathway, which results in diminished c-Jun steady state levels in HeLa cells. The aim of this work was to search for new CSN kinase inhibitors analogue to curcumin and emodin by means of an in silico screening method. METHODS: Here we present a novel method to identify efficient inhibitors of CSN-associated kinases. Using curcumin and emodin as lead structures an in silico screening with our in-house database containing more than 10(6 )structures was carried out. Thirty-five compounds were identified and further evaluated by the Lipinski's rule-of-five. Two groups of compounds can be clearly discriminated according to their structures: the curcumin-group and the emodin-group. The compounds were evaluated in in vitro kinase assays and in cell culture experiments. RESULTS: The data revealed 3 compounds of the curcumin-group (e.g. piceatannol) and 4 of the emodin-group (e.g. anthrachinone) as potent inhibitors of CSN-associated kinases. Identified agents increased p53 levels and induced apoptosis in tumor cells as determined by annexin V-FITC binding, DNA fragmentation and caspase activity assays. CONCLUSION: Our data demonstrate that the new in silico screening method is highly efficient for identifying potential anti-tumor drugs

An Aqueous Extract of Fagonia cretica Induces DNA Damage, Cell Cycle Arrest and Apoptosis in Breast Cancer Cells via FOXO3a and p53 Expression

Background - Plants have proved to be an important source of anti-cancer drugs. Here we have investigated the cytotoxic action of an aqueous extract of Fagonia cretica, used widely as a herbal tea-based treatment for breast cancer. Methodology/Principal Findings - Using flow cytometric analysis of cells labeled with cyclin A, annexin V and propidium iodide, we describe a time and dose-dependent arrest of the cell cycle in G0/G1 phase of the cell cycle and apoptosis following extract treatment in MCF-7 (WT-p53) and MDA-MB-231 (mutant-p53) human breast cancer cell lines with a markedly reduced effect on primary human mammary epithelial cells. Analysis of p53 protein expression and of its downstream transcription targets, p21 and BAX, revealed a p53 associated growth arrest within 5 hours of extract treatment and apoptosis within 24 hours. DNA double strand breaks measured as ?-H2AX were detected early in both MCF-7 and MDA-MB-231 cells. However, loss of cell viability was only partly due to a p53-driven response; as MDA-MB-231 and p53-knockdown MCF-7 cells both underwent cell cycle arrest and death following extract treatment. p53-independent growth arrest and cytotoxicity following DNA damage has been previously ascribed to FOXO3a expression. Here, in MCF-7 and MDA-MB-231 cells, FOXO3a expression was increased significantly within 3 hours of extract treatment and FOXO3 siRNA reduced the extract-induced loss of cell viability in both cell lines. Conclusions/Significance - Our results demonstrate for the first time that an aqueous extract of Fagonia cretica can induce cell cycle arrest and apoptosis via p53-dependent and independent mechanisms, with activation of the DNA damage response. We also show that FOXO3a is required for activity in the absence of p53. Our findings indicate that Fagonia cretica aqueous extract contains potential anti-cancer agents acting either singly or in combination against breast cancer cell proliferation via DNA damage-induced FOXO3a and p53 expression

Listening in first and second language

Listeners' recognition of spoken language involves complex decoding processes: The continuous speech stream must be segmented into its component words, and words must be recognized despite great variability in their pronunciation (due to talker differences, or to influence of phonetic context, or to speech register) and despite competition from many spuriously present forms supported by the speech signal. L1 listeners deal more readily with all levels of this complexity than L2 listeners. Fortunately, the decoding processes necessary for competent L2 listening can be taught in the classroom. Evidence-based methodologies targeted at the development of efficient speech decoding include teaching of minimal pairs, of phonotactic constraints, and of reduction processes, as well as the use of dictation and L2 video captions