35 research outputs found

    Apollo 14: Some geochemical aspects

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    Chemical analyses were obtained for five samples of Apollo 14 regolith fines, three 14230 core samples, soil clod 14049, breccias 14305 and 14319, 14310 basalt, and some separated phases. The chemical uniformity of these Apollo 14 samples indicates thorough mixing and/or uniform source rocks. Basalt 14310 can be matched well in composition by a four to one mixture of soil and plagioclase. The Eu(2+)/Eu(3+) ratios calculated for 14310 pigeonite and plagioclase are similar to those for Apollo 12 and 15 mare-type basalt phases; this indicates similar redox conditions. Apollo 14 samples are chemically similar to Apollo 12 and 15 KREEP as distinct from Apollo 11, 12, and 15 and Luna 16 mare-type basalts

    Requirement of α and β subunit transmembrane helix separation for integrin outside-in signaling

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    Adhesion to extracellular ligands through integrins regulates cell shape, migration, growth, and survival. How integrins transmit signals in the outside-to-in direction remains unknown. Whereas in resting integrins the α and β subunit transmembrane domains are associated, ligand binding promotes dissociation and separation of these domains. Here we address whether such separation is required for outside-in signaling. By introduction of an intersubunit disulfide bond, we generated mutant integrin αIIbβ3 with blocked transmembrane separation that binds ligand, mediates adhesion, adopts an extended conformation after ligand binding, and forms antibody-induced macroclusters on the cell surface similarly to wild type. However, the mutant integrin exhibits a profound defect in adhesion-induced outside-in signaling as measured by cell spreading, actin stress-fiber and focal adhesion formation, and focal adhesion kinase activation. This defect was rescued by reduction of the disulfide bond. Our results demonstrate that the separation of transmembrane domains is required for integrin outside-in signal transduction
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