196 research outputs found
Anion Formation of 4β-(Dimethylamino)-3-hydroxyflavone in Phosphatidylglycerol Vesicles Induced by HEPES Buffer: A Steady-State and Time-Resolved Fluorescence Investigation
Solvatochromic and Fluorogenic Dyes as Environment-Sensitive Probes: Design and Biological Applications.
Fluorescent environment-sensitive probes are specially designed dyes that change their fluorescence intensity (fluorogenic dyes) or color (e.g., solvatochromic dyes) in response to change in their microenvironment polarity, viscosity, and molecular order. The studies of the past decade, including those of our group, have shown that these molecules become universal tools in fluorescence sensing and imaging. In fact, any biomolecular interaction or change in biomolecular organization results in modification of the local microenvironment, which can be directly monitored by these types of probes. In this Account, the main examples of environment-sensitive probes are summarized according to their design concepts. Solvatochromic dyes constitute a large class of environment-sensitive probes which change their color in response to polarity. Generally, they are push-pull dyes undergoing intramolecular charge transfer. Emission of their highly polarized excited state shifts to the red in more polar solvents. Excited-state intramolecular proton transfer is the second key concept to design efficient solvatochromic dyes, which respond to the microenvironment by changing relative intensity of the two emissive tautomeric forms. Due to their sensitivity to polarity and hydration, solvatochromic dyes have been successfully applied to biological membranes for studying lipid domains (rafts), apoptosis and endocytosis. As fluorescent labels, solvatochromic dyes can detect practically any type of biomolecular interactions, involving proteins, nucleic acids and biomembranes, because the binding event excludes local water molecules from the interaction site. On the other hand, fluorogenic probes usually exploit intramolecular rotation (conformation change) as a design concept, with molecular rotors being main representatives. These probes were particularly efficient for imaging viscosity and lipid order in biomembranes as well as to light up biomolecular targets, such as antibodies, aptamers and receptors. The emerging concepts to achieve fluorogenic response to the microenvironment include ground-state isomerization, aggregation-caused quenching, and aggregation-induced emission. The ground-state isomerization exploits, for instance, polarity-dependent spiro-lactone formation in silica-rhodamines. The aggregation-caused quenching uses disruption of the self-quenched dimers and nanoassemblies of dyes in less polar environments of lipid membranes and biomolecules. The aggregation-induced emission couples target recognition with formation of highly fluorescent dye aggregates. Overall, solvatochromic and fluorogenic probes enable background-free bioimaging in wash-free conditions as well as quantitative analysis when combined with advanced microscopy, such as fluorescence lifetime (FLIM) and ratiometric imaging. Further development of fluorescent environment-sensitive probes should address some remaining problems: (i) improving their optical properties, especially brightness, photostability, and far-red to near-infrared operating range; (ii) minimizing nonspecific interactions of the probes in biological systems; (iii) their adaptation for advanced microscopies, notably for superresolution and in vivo imaging.journal article2017 Feb 212017 01 09importe
Characterization of Coupled Ground State and Excited State Equilibria by Fluorescence Spectral Deconvolution
Fluorescence probes with multiparametric response based on the relative variation in the intensities of several emission bands are of great general utility. An accurate interpretation of the system requires the determination of the number, positions and intensities of the spectral components. We have developed a new algorithm for spectral deconvolution that is applicable to fluorescence probes exhibiting a two-state ground-state equilibrium and a two-state excited-state reaction. Three distinct fluorescence emission bands are resolved, with a distribution of intensities that is excitation-wavelength-dependent. The deconvolution of the spectrum into individual components is based on their representation as asymmetric Siano-Metzler log-normal functions. The application of the algorithm to the solvation response of a 3-hydroxychromone (3HC) derivative that exhibits an H-bonding-dependent excited-state intramolecular proton transfer (ESIPT) reaction allowed the separation of the spectral signatures characteristic of polarity and hydrogen bonding. This example demonstrates the ability of the method to characterize two potentially uncorrelated parameters characterizing dye environment and interactions
Characterizing Counterion-Dependent Aggregation of Rhodamine B by Classical Molecular Dynamics Simulations
The aggregation in a solution of charged dyes such as Rhodamine B (RB) is significantly affected by the type of counterion, which can determine the self-assembled structure that in turn modulates the optical properties. RB aggregation can be boosted by hydrophobic and bulky fluorinated tetraphenylborate counterions, such as F5TPB, with the formation of nanoparticles whose fluorescence quantum yield (FQY) is affected by the degree of fluorination. Here, we developed a classical force field (FF) based on the standard generalized Amber parameters that allows modeling the self-assembling process of RB/F5TPB systems in water, consistent with experimental evidence. Namely, the classical MD simulations employing the re-parametrized FF reproduce the formation of nanoparticles in the RB/F5TPB system, while in the presence of iodide counterions, only RB dimeric species can be formed. Within the large, self-assembled RB/F5TPB aggregates, the occurrence of an H-type RB-RB dimer can be observed, a species that is expected to quench RB fluorescence, in agreement with the experimental data of FQY. The outcome provides atomistic details on the role of the bulky F5TPB counterion as a spacer, with the developed classical FF representing a step towards reliable modeling of dye aggregation in RB-based materials
Ca-NIR: a ratiometric near-infrared calcium probe based on a dihydroxanthene-hemicyanine fluorophore.
Fluorescent calcium probes are essential tools for studying the fluctuation of calcium ions in cells. Herein, we developed Ca-NIR, the first ratiometric calcium probe emitting in the near infrared region. This probe arose from the fusion of a BAPTA chelator and a dihydroxanthene-hemicyanine fluorophore. It is efficiently excited with common 630-640 nm lasers and displays two distinct emission bands depending on the calcium concentration (Kd = βΌ8 ΞΌM). The physicochemical and spectroscopic properties of Ca-NIR allowed for ratiometric imaging of calcium distribution in live cells.journal article2017 Jun 01imported"Supporting information" disponible sur le site de l'Γ©diteu
Two-Dimensional Molecular Patterning by Surface-Enhanced Zn-Porphyrin Coordination
In this contribution, we show how zinc-5,10,15,20-meso-tetradodecylporphyrins (Zn-TDPs) self-assemble into stable organized arrays on the surface of graphite, thus positioning their metal center at regular distances from each other, creating a molecular pattern, while retaining the possibility to coordinate additional ligands. We also demonstrate that Zn-TDPs coordinated to 3-nitropyridine display a higher tendency to be adsorbed at the surface of highly oriented pyrolytic graphite (HOPG) than noncoordinated ones. In order to investigate the two-dimensional (2D) self-assembly of coordinated Zn-TDPs, solutions with different relative concentrations of 3-nitropyridine and Zn-TDP were prepared and deposited on the surface of HOPG. STM measurements at the liquid-solid interface reveal that the ratio of coordinated Zn-TDPs over noncoordinated Zn-TDPs is higher at the n-tetradecane/HOPG interface than in n-tetradecane solution. This enhanced binding of the axial ligand at the liquid/solid interface is likely related to the fact that physisorbed Zn-TDPs are better binding sites for nitropyridines.
ΠΠΎΠΌΠΏΠ»Π΅ΠΊΡΠΎΡΡΠ²ΠΎΡΠ΅Π½Π½Ρ ΠΊΠ°Π»ΡΠΊΡ[4]Π°ΡΠ΅Π½Π³ΡΠ΄ΡΠΎΠΊΡΠΈΠΌΠ΅ΡΠΈΠ»-ΡΠΎΡΡΠΎΠ½ΠΎΠ²ΠΎΡ ΠΊΠΈΡΠ»ΠΎΡΠΈ Π· ΡΡΠΈΠΏΡΠΎΡΠ°Π½ΠΎΠΌ ΡΠ° N-aΡΠ΅ΡΠΈΠ»-ΡΡΠΈΠΏΡΠΎΡΠ°Π½Π°ΠΌΡΠ΄ΠΎΠΌ
The Host-Guest complexation of calixarene hydroxymethylphosphonic acid with tryptophan and N-acetyltryptophan amide has been investigated by the RP HPLC method in H2O/MeCN (99/1) solution (column support Hypersil CN, UV-detector, Ξ» = 254 nm). Adsorption of calixarene hydroxymethylphosphonic acid on the Hypersil CN surface has been studied. It has been found that hydroxymethylphosphonic acid is characterized by reversible sorption on the Hypersil CN surface. The binding constants (KA = 23000 M-1 and 39000 M-1 for tryptophan and N-acetyltryptophan amide, respectively) of the supramolecular complexes have been calculated from the ratio between the capacity factors kβ of the Guest and the calixarene hydroxymethylphosphonic acid Host concentration in the mobile phase. The Gibbs free energies of the tryptophan and N-acetyltryptophan amide complexes are -24.84 and -26.15 kJ/mol, respectively. The molecular modelling of calixarene hydroxymethylphosphonic acid and its complexes with tryptophan and N-acetyltryptophan amide (Hyper Chem, version 8, force field PM3) has indicated that the complexes are stabilized by hydrogen bonds, electrostatic, Ο-Ο, and solvatophobic interactions. The geometric parameters of the energy minimized calixarene macrocycle and its complexes with tryptophan and N-acetyltryptophan amide have been calculated. According to the calculations it has been shown that the Host-Guest complexation does not change the flattened-cone conformation of calixarene. Finally, the inverse correlation has been found between the KA values of the complexes and the Log P values of the guest molecules.ΠΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΠ€ ΠΠΠΠ₯ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ ΠΏΡΠΎΡΠ΅ΡΡ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠΎΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡ ΡΠΈΠΏΠ° Π₯ΠΎΠ·ΡΠΈΠ½-ΠΠΎΡΡΡ ΠΊΠ°Π»ΠΈΠΊΡΠ°ΡΠ΅Π½Π³ΠΈΠ΄ΡΠΎΠΊΡΠΈΠΌΠ΅ΡΠΈΠ»ΡΠΎΡΡΠΎΠ½ΠΎΠ²ΠΎΠΉ ΠΊΠΈΡΠ»ΠΎΡΡ Ρ ΡΡΠΈΠΏΡΠΎΡΠ°Π½ΠΎΠΌ ΠΈ N-aΡΠ΅ΡΠΈΠ»-ΡΡΠΈΠΏΡΠΎΡΠ°Π½Π°ΠΌΠΈΠ΄ΠΎΠΌ Π² ΡΠ°ΡΡΠ²ΠΎΡΠ΅ H2O/MeCN (99/1) (Π½Π°ΡΠ°Π΄ΠΊΠ° Hypersil CN, Π£Π€-Π΄Π΅ΡΠ΅ΠΊΡΠΎΡ, Ξ» = 254 Π½ΠΌ). ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΎ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ ΠΊΠ°Π»ΠΈΠΊΡΠ°ΡΠ΅Π½Π³ΠΈΠ΄ΡΠΎΠΊΡΠΈΠΌΠ΅ΡΠΈΠ»ΡΠΎΡΡΠΎΠ½ΠΎΠ²ΠΎΠΉ ΠΊΠΈΡΠ»ΠΎΡΡ Ρ ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΡΡ Ρ
ΡΠΎΠΌΠ°ΡΠΎΠ³ΡΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ Π½Π°ΡΠ°Π΄ΠΊΠΈ Hypersil CN. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ ΠΊΠ°Π»ΠΈΠΊΡΠ°ΡΠ΅Π½Π³ΠΈΠ΄ΡΠΎΠΊΡΠΈΠΌΠ΅ΡΠΈΠ»ΡΠΎΡΡΠΎΠ½ΠΎΠ²Π°Ρ ΠΊΠΈΡΠ»ΠΎΡΠ° Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΠ·ΡΠ΅ΡΡΡ ΠΎΠ±ΡΠ°ΡΠΈΠΌΠΎΠΉ ΡΠΎΡΠ±ΡΠΈΠ΅ΠΉ Π½Π° ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠΈ Hypersil CN. ΠΠΎΠ½ΡΡΠ°Π½ΡΡ ΡΠ²ΡΠ·ΡΠ²Π°Π½ΠΈΡ ΡΡΠΏΡΠ°ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΡ
ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠΎΠ² (23000 M-1 ΠΈ 39000 M-1 Π΄Π»Ρ ΡΡΠΈΠΏΡΠΎΡΠ°Π½Π° ΠΈ N-aΡΠ΅ΡΠΈΠ»ΡΡΠΈΠΏΡΠΎΡΠ°Π½Π°ΠΌΠΈΠ΄Π°, ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ) Π±ΡΠ»ΠΈ ΡΠ°ΡΡΡΠΈΡΠ°Π½Ρ ΠΈΠ· ΡΠΎΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΡ ΠΌΠ΅ΠΆ- Π΄Ρ ΠΊΠΎΡΡΡΠΈΡΠΈΠ΅Π½ΡΠΎΠΌ Π΅ΠΌΠΊΠΎΡΡΠΈ kβ ΠΌΠΎΠ»Π΅ΠΊΡΠ»Ρ ΠΠΎΡΡΡ ΠΈ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠ΅ΠΉ ΠΊΠ°Π»ΠΈΠΊΡΠ°ΡΠ΅Π½Π³ΠΈΠ΄ΡΠΎΠΊΡΠΈΠΌΠ΅ΡΠΈΠ»ΡΠΎΡΡΠΎΒΠ½ΠΎΠ²ΠΎΠΉ ΠΊΠΈΡΠ»ΠΎΡΡ Π₯ΠΎΠ·ΡΠΈΠ½Π° Π² ΠΏΠΎΠ΄Π²ΠΈΠΆΠ½ΠΎΠΉ ΡΠ°Π·Π΅. ΠΠ½Π°ΡΠ΅Π½ΠΈΡ ΡΠ²ΠΎΠ±ΠΎΠ΄Π½ΡΡ
ΡΠ½Π΅ΡΠ³ΠΈΠΉ ΠΠΈΠ±Π±ΡΠ° ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠΎΠ² ΠΊΠ°Π»ΠΈΠΊΡΠ°ΒΡΠ΅Π½Π³ΠΈΠ΄ΡΠΎΠΊΡΠΈΠΌΠ΅ΡΠΈΠ»ΡΠΎΡΡΠΎΠ½ΠΎΠ²ΠΎΠΉ ΠΊΠΈΡΠ»ΠΎΡΡ Ρ ΡΡΠΈΠΏΡΠΎΡΠ°Π½ΠΎΠΌ ΠΈ N-aΡΠ΅ΡΠΈΠ»-ΡΡΠΈΠΏΡΠΎΡΠ°Π½Π°ΠΌΠΈΠ΄ΠΎΠΌ ΡΠΎΡΡΠ°Π²ΠΈΠ»ΠΈ -24.84 ΠΈ -26.15 ΠΊΠΠΆ/ΠΌΠΎΠ»Ρ, ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ. ΠΡΠΎΠ²Π΅Π΄Π΅Π½ΠΎ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΠΎΠ΅ ΠΌΠΎΠ΄Π΅Π»ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΊΠ°Π»ΠΈΠΊΡΠ°ΡΠ΅Π½Π³ΠΈΠ΄ΡΠΎΠΊΡΠΈ- ΠΌΠ΅ΡΠΈΠ»ΡΠΎΡΡΠΎΠ½ΠΎΠ²ΠΎΠΉ ΠΊΠΈΡΠ»ΠΎΡΡ ΠΈ Π΅Π΅ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠΎΠ² Ρ ΡΡΠΈΠΏΡΠΎΡΠ°Π½ΠΎΠΌ ΠΈ N-aΡΠ΅ΡΠΈΠ»ΡΡΠΈΠΏΡΠΎΡΠ°Π½Π°ΠΌΠΈΠ΄ΠΎΠΌ (Hyper Chem, Π²Π΅ΡΡΠΈΡ 8, ΡΠΈΠ»ΠΎΠ²ΠΎΠ΅ ΠΏΠΎΠ»Π΅ PM3). ΠΡΠΌΠ΅ΡΠ°Π΅ΡΡΡ, ΡΡΠΎ ΡΡΠΏΡΠ°ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΠ΅ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΡ ΠΌΠΎΠ³ΡΡ ΡΡΠ°Π±ΠΈΠ»ΠΈΠ·ΠΈΡΠΎΠ²Π°ΡΡΡΡ Π²ΠΎΠ΄ΠΎΡΠΎΠ΄Π½ΡΠΌΠΈ ΡΠ²ΡΠ·ΡΠΌΠΈ, Π° ΡΠ°ΠΊΠΆΠ΅ ΡΠ»Π΅ΠΊΡΡΠΎΡΡΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ, Ο-Ο, ΠΈ ΡΠΎΠ»ΡΠ²Π°ΡΠΎΡΠΎΠ±Π½ΡΠΌΠΈ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΡΠΌΠΈ. Π Π°ΡΡΡΠΈΡΠ°Π½Ρ Π³Π΅ΠΎΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΡ ΡΠ½Π΅ΡΠ³Π΅ΡΠΈΡΠ΅ΡΠΊΠΈ ΠΌΠΈΠ½ΠΈΠΌΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
ΡΡΡΡΠΊΡΡΡ ΠΊΠ°Π»ΠΈΠΊΡΠ°ΡΠ΅Π½Π³ΠΈΠ΄ΡΠΎΠΊΡΠΈΠΌΠ΅ΡΠΈΠ»ΡΠΎΡΡΠΎΠ½ΠΎΠ²ΠΎΠΉ ΠΊΠΈΡΠ»ΠΎΡΡ ΠΈ Π΅Π΅ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠΎΠ² Ρ ΡΡΠΈΠΏΡΠΎΡΠ°Π½ΠΎΠΌ ΠΈ N-aΡΠ΅ΡΠΈΠ»ΡΡΠΈΠΏΡΠΎΡΠ°Π½Π°ΠΌΠΈΠ΄ΠΎΠΌ. Π‘ΠΎΠ³Π»Π°ΡΠ½ΠΎ ΡΠ°ΡΡΠ΅ΡΠ°ΠΌ ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΠΏΡΠΎΡΠ΅ΡΡ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠΎΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡ Π½Π΅ ΠΌΠ΅Π½ΡΠ΅Ρ ΠΊΠΎΠ½ΡΠΎΡΠΌΠ°ΡΠΈΡ ΠΌΠ°ΠΊΡΠΎΡΠΈΠΊΠ»ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΎΡΡΠΎΠ²Π° ΠΊΠ°Π»ΠΈΠΊΡΠ°ΡΠ΅Π½Π°. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ Π·Π½Π°ΡΠ΅Π½ΠΈΡ KA ΠΏΠΎΠ²ΡΡΠ°ΡΡΡΡ ΡΠΎ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΠ΅ΠΌ Log P ΠΌΠΎΠ»Π΅ΠΊΡΠ» ΠΠΎΡΡΠ΅ΠΉ.ΠΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΠ€ ΠΠΠ Π₯ Π΄ΠΎΡΠ»ΡΠ΄ΠΆΠ΅Π½ΠΎ ΠΏΡΠΎΡΠ΅Ρ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠΎΡΡΠ²ΠΎΡΠ΅Π½Π½Ρ ΡΠΈΠΏΡ ΠΠΎΡΠΏΠΎΠ΄Π°Ρ-ΠΡΡΡΡ ΠΊΠ°Π»ΡΠΊΡΠ°ΡΠ΅Π½Π³ΡΠ΄ΡΠΎΠΊΡΠΈ- ΠΌΠ΅ΡΠΈΠ»ΡΠΎΡΡΠΎΠ½ΠΎΠ²ΠΎΡ ΠΊΠΈΡΠ»ΠΎΡΠΈ Π· ΡΡΠΈΠΏΡΠΎΡΠ°Π½ΠΎΠΌ ΡΠ° N-aΡΠ΅ΡΠΈΠ»ΡΡΠΈΠΏΡΠΎΡΠ°Π½Π°ΠΌΡΠ΄ΠΎΠΌ Ρ ΡΠΎΠ·ΡΠΈΠ½Ρ H2O/MeCN (99/1) (Π½Π°ΡΠ°Π΄ΠΊΠ° Hypersil CN, Π£Π€-Π΄Π΅ΡΠ΅ΠΊΡΠΎΡ, Ξ» = 254 Π½ΠΌ). ΠΠΎΡΠ»ΡΠ΄ΠΆΠ΅Π½ΠΎ Π²Π·Π°ΡΠΌΠΎΠ΄ΡΡ ΠΊΠ°Π»ΡΠΊΡΠ°ΡΠ΅Π½Π³ΡΠ΄ΡΠΎΠΊΡΠΈΠΌΠ΅ΡΠΈΠ»ΡΠΎΡΡΠΎΠ½ΠΎΠ²ΠΎΡ ΠΊΠΈΡΠ»ΠΎΡΠΈ Π· ΠΏΠΎΠ²Π΅ΡΡ
Π½Π΅Ρ Ρ
ΡΠΎΠΌΠ°ΡΠΎΠ³ΡΠ°ΡΡΡΠ½ΠΎΡ Π½Π°ΡΠ°Π΄ΠΊΠΈ Hypersil CN. ΠΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΠΎ ΠΊΠ°Π»ΡΠΊΡΠ°ΡΠ΅Π½Π³ΡΠ΄ΡΠΎΠΊΡΠΈΠΌΠ΅ΡΠΈΠ»ΡΠΎΡΡΠΎΠ½ΠΎΠ²Π° ΠΊΠΈΡΠ»ΠΎΡΠ° Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΠ·ΡΡΡΡΡΡ ΠΎΠ±Π΅ΡΠ½Π΅Π½ΠΎΡ ΡΠΎΡΠ±ΡΡΡΡ Π½Π° ΠΏΠΎΠ²Π΅ΡΡ
Π½Ρ Hypersil CN. ΠΠΎΠ½- ΡΡΠ°Π½ΡΠΈ Π·Π²βΡΠ·ΡΠ²Π°Π½Π½Ρ ΡΡΠΏΡΠ°ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΠΈΡ
ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΡΠ² (23000 M-1 Ρ 39000 M-1 Π΄Π»Ρ ΡΡΠΈΠΏΡΠΎΡΠ°Π½Ρ Ρ N-aΡΠ΅ΡΠΈΠ»ΡΡΠΈΠΏΡΠΎΡΠ°Π½Π°ΠΌΡΠ΄Ρ, Π²ΡΠ΄ΠΏΠΎΠ²ΡΠ΄Π½ΠΎ) Π±ΡΠ»ΠΈ ΡΠΎΠ·ΡΠ°Ρ
ΠΎΠ²Π°Π½Ρ ΡΠ· ΡΠΏΡΠ²Π²ΡΠ΄Π½ΠΎΡΠ΅Π½Π½Ρ ΠΌΡΠΆ ΠΊΠΎΠ΅ΡΡΡΡΡΠ½ΡΠΎΠΌ ΡΠΌΠΊΠΎΡΡΡ kβ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΠΈ ΠΠΎΡΡΡ Ρ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΡΡΡ ΠΊΠ°Π»ΡΠΊΡΠ°ΡΠ΅Π½Π³ΡΠ΄ΡΠΎΠΊΡΠΈΠΌΠ΅ΡΠΈΠ»ΡΠΎΡΡΠΎΠ½ΠΎΠ²ΠΎΡ ΠΊΠΈΡΠ»ΠΎΡΠΈ ΠΠΎΡΠΏΠΎΠ΄Π°ΡΡ Π² ΡΡΡ
ΠΎΠΌΡΠΉ ΡΠ°Π·Ρ. ΠΠ½Π°ΡΠ΅Π½Π½Ρ Π²ΡΠ»ΡΠ½ΠΈΡ
ΡΠ½Π΅ΡΠ³ΡΠΉ ΠΡΠ±Π±ΡΠ° ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΡΠ² ΠΊΠ°Π»ΡΠΊΡΠ°ΡΠ΅Π½Π³ΡΠ΄ΡΠΎΠΊΡΠΈΠΌΠ΅ΡΠΈΠ»ΡΠΎΡΡΠΎΠ½ΠΎΠ²ΠΎΡ ΠΊΠΈΡΠ»ΠΎΡΠΈ Π· ΡΡΠΈΠΏΡΠΎΡΠ°Π½ΠΎΠΌ Ρ N-aΡΠ΅- ΡΠΈΠ»ΡΡΠΈΠΏΡΠΎΡΠ°Π½Π°ΠΌΡΠ΄ΠΎΠΌ ΡΠΊΠ»Π°Π΄Π°Ρ -24.84 Ρ -26.15 ΠΊΠΠΆ/ΠΌΠΎΠ»Ρ, Π²ΡΠ΄ΠΏΠΎΠ²ΡΠ΄Π½ΠΎ. ΠΠ΄ΡΠΉΡΠ½Π΅Π½ΠΎ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½Π΅ ΠΌΠΎΠ΄Π΅Π»ΡΠ²Π°Π½Π½Ρ ΠΊΠ°Π»ΡΠΊΡΠ°ΡΠ΅Π½Π³ΡΠ΄ΡΠΎΠΊΡΠΈΠΌΠ΅ΡΠΈΠ»ΡΠΎΡΡΠΎΠ½ΠΎΠ²ΠΎΡ ΠΊΠΈΡΠ»ΠΎΡΠΈ Ρ ΡΡ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΡΠ² Π· ΡΡΠΈΠΏΡΠΎΡΠ°Π½ΠΎΠΌ Ρ N-aΡΠ΅ΡΠΈΠ»ΡΡΠΈΠΏΡΠΎΡΠ°Π½Π°ΠΌΡΠ΄ΠΎΠΌ (Hyper Chem, Π²Π΅ΡΡΡΡ 8, ΡΠΈΠ»ΠΎΠ²Π΅ ΠΏΠΎΠ»Π΅ PM3). Π‘ΡΠΏΡΠ°ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½Ρ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠΈ ΠΌΠΎΠΆΡΡΡ ΡΡΠ°Π±ΡΠ»ΡΠ·ΡΠ²Π°ΡΠΈΡΡ Π²ΠΎΠ΄Π½Π΅Π²ΠΈΠΌΠΈ Π·Π²βΡΠ·ΠΊΠ°ΠΌΠΈ, Π° ΡΠ°ΠΊΠΎΠΆ Π΅Π»Π΅ΠΊΡΡΠΎΡΡΠ°ΡΠΈΡΠ½ΠΈΠΌΠΈ, Ο-Ο, Ρ ΡΠΎΠ»ΡΠ²Π°ΡΠΎΡΠΎΠ±Π½ΠΈΠΌΠΈ Π²Π·Π°ΡΠΌΠΎΠ΄ΡΡΠΌΠΈ. Π ΠΎΠ·ΡΠ°Ρ
ΠΎΠ²Π°Π½Ρ Π³Π΅ΠΎΠΌΠ΅ΡΡΠΈΡΠ½Ρ ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΠΈ ΡΠ½Π΅ΡΠ³Π΅ΡΠΈΡΠ½ΠΎ ΠΌΡΠ½ΡΠΌΡΠ·ΠΎΠ²Π°Π½ΠΈΡ
ΡΡΡΡΠΊΡΡΡ ΠΊΠ°Π»ΡΠΊΡΠ°ΡΠ΅Π½Π³ΡΠ΄ΡΠΎΠΊΡΠΈΠΌΠ΅ΡΠΈΠ»ΡΠΎΡΡΠΎΠ½ΠΎΠ²ΠΎΡ ΠΊΠΈΡ- Π»ΠΎΡΠΈ ΡΠ° ΡΡ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΡΠ² Π· ΡΡΠΈΠΏΡΠΎΡΠ°Π½ΠΎΠΌ Ρ N-aΡΠ΅ΡΠΈΠ»ΡΡΠΈΠΏΡΠΎΡΠ°Π½Π°ΠΌΡΠ΄ΠΎΠΌ. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΠΎ Π·Π½Π°ΡΠ΅Π½Π½Ρ KA Π·ΡΠΎΡΒΡΠ°ΡΡΡ Π·Ρ Π·Π½ΠΈΠΆΠ΅Π½Π½ΡΠΌ Log P ΠΌΠΎΠ»Π΅ΠΊΡΠ» ΡΡΠ±ΡΡΡΠ°ΡΡΠ², Π° ΠΏΡΠΎΡΠ΅Ρ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠΎΡΡΠ²ΠΎΡΠ΅Π½Π½Ρ Π½Π΅ Π·ΠΌΡΠ½ΡΡ ΠΊΠΎΠ½ΡΠΎΡΠΌΠ°ΡΡΡ ΠΌΠ°ΠΊΡΠΎΡΠΈΠΊΠ»ΡΡΠ½ΠΎΠ³ΠΎ ΠΊΡΡΡΡΠΊΠ° ΠΊΠ°Π»ΡΠΊΡΠ°ΡΠ΅Π½Ρ
Simultaneous computer-assisted assessment of mucosal and serosal perfusion in a model of segmental colonic ischemia
BACKGROUND: Fluorescence-based enhanced reality (FLER) enables the quantification of fluorescence signal dynamics, which can be superimposed onto real-time laparoscopic images by using a virtual perfusion cartogram. The current practice of perfusion assessment relies on visualizing the bowel serosa. The aim of this experimental study was to quantify potential differences in mucosal and serosal perfusion levels in an ischemic colon segment. METHODS: An ischemic colon segment was created in 12 pigs. Simultaneous quantitative mucosal and serosal fluorescence imaging was obtained via intravenous indocyanine green injection (0.2Β mg/kg), using two near-infrared camera systems, and computer-assisted FLER analysis. Lactate levels were measured in capillary blood of the colonic wall at seven regions of interest (ROIs) as determined with FLER perfusion cartography: the ischemic zone (I), the proximal and distal vascularized areas (PV, DV), and the 50% perfusion threshold proximally and distally at the mucosal and serosal side (P50M, P50S, D50M, D50S). RESULTS: The mean ischemic zone as measured (mm) for the mucosal side was significantly larger than the serosal one (56.3βΒ±β21.3 vs. 40.8βΒ±β14.9, pβ=β0.001) with significantly lower lactate values at the mucosal ROIs. There was a significant weak inverse correlation between lactate and slope values for the defined ROIs (rβ=β-Β 0.2452, pβ=β0.0246). CONCLUSIONS: Mucosal ischemic zones were larger than serosal zones. These results suggest that an assessment of bowel perfusion from the serosal side only can underestimate the extent of ischemia. Further studies are required to predict the optimal resection margin and anastomotic site
Photopolymerized micelles of diacetylene amphiphile: physical characterization and cell delivery properties:
A series of polydiacetylene (PDA) - based micelles were prepared from diacetylenic surfactant bearing polyethylene glycol, by increasing UV-irradiation times. These polymeric lipid micelles were analyzed by physicochemical methods, electron microscopy and NMR analysis. Cellular delivery of fluorescent dye suggests that adjusting the polymerization state is vital to reach the full in vitro potential of PDA-based delivery system
Non-coordinating anions assemble cyanine amphiphiles into ultra-small fluorescent nanoparticles
A non-coordinating anion, fluorinated tetraphenylborate, assembles specially designed cationic cyanine amphiphiles into 7β8 nm fluorescent nanoparticles that are >40-fold brighter than a single cyanine dye. This kind of anion, combining hydrophobic and electrostatic forces in aqueous media, constitutes promising building blocks in the self-assembly of functional nanomaterials
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