Die konsep van biobeskikbaarheid en die neerlê van eenvormige standaarde vir toelaatbare chemiese kontaminante in die grond

The original publication is available at http://www.satnt.ac.za/Soils are very heterogeneous substrates providing an environmental matrix with varying spatial and temporal gradients of pH, organic carbon, particle size distribution, moisture content as well as biological factors associated with soil organisms. These chemical, physical as well as biological factors determine the bioavailability of chemicals to soil-dwelling invertebrates. This review of recent literature on the use of the bioavailability concept in soil ecotoxicology indicates that the concept is often used unqualified and indiscriminately to mean different things to different authors. A clear understanding of the concept is crucial for toxicity testing, environmental monitoring, risk assessment and the setting of soil quality criteria since knowledge of the actual exposure of organisms, and not merely the total amount of the chemical, is required. The aim of this paper is to contribute towards a clarification of the concept. Apart from defining or describing bioavailability, the problems related to the comparability of toxicity data between soils and species, inter-soil and inter-species comparisons of toxicity data are discussed. The potential role that biomarkers can play in assessing bioavailability, is touched upon. In an effort to prescribe uniform criteria or standards for environmental quality, both biotic and abiotic characteristics, which determine the bioavailablity of contaminants should be considered. This requires a dynamic approach which takes both uptake processes as well as a variety of other biological factors into consideration. It is concluded that bioavialiblity should be interpreted qualitatively and that the rate of uptake of a contaminant could possibly serve as a measure of bioavailability. The development of standardised protocols for exposure of selected species and the measurement of biological responses with the aid of biomarkers could serve to refine and take risk assessment a step further.Publishers' Versio

The concept of bioavailability and establishing uniform standards for permissible chemical contamination of soil

The original publication is available at http://www.satnt.ac.zaSoils are very heterogeneous substrates providing an environmental matrix with varying spatial and temporal gradients of pH, organic carbon, particle size distribution, moisture content as well as biological factors associated with soil organisms. These chemical, physical as well as biological factors determine the bioavailability of chemicals to soil-dwelling invertebrates. This review of recent literature on the use of the bioavailability concept in soil ecotoxicology indicates that the concept is often used unqualified and indiscriminately to mean different things to different authors. A clear understanding of the concept is crucial for toxicity testing, environmental monitoring, risk assessment and the setting of soil quality criteria since knowledge of the actual exposure of organisms, and not merely the total amount of the chemical, is required. The aim of this paper is to contribute towards a clarification of the concept. Apart from defining or describing bioavailability, the problems related to the comparability of toxicity data between soils and species, inter-soil and inter-species comparisons of toxicity data are discussed. The potential role that biomarkers can play in assessing bioavailability, is touched upon. In an effort to prescribe uniform criteria or standards for environmental quality, both biotic and abiotic characteristics, which determine the bioavailablity of contaminants, should be considered. This requires a dynamic approach which takes both uptake processes as well as a variety of other biological factors into consideration. It is concluded that bioavialiblity should be interpreted qualitatively and that the rate of uptake of a contaminant could possibly serve as a measure of bioavailability. The development of standardised protocols for exposure of selected species and the measurement of biological responses with the aid of biomarkers could serve to refine and take risk assessment a step further.Grond is ’n besonder heterogene substraat en ’n matriks vir variërende ruimtelike en temporale gradiënte van pH, organiese koolstof, partikelgroottes, voginhoude asook biologiese faktore geassosieer met grondlewende organismes. Hierdie chemiese, fisiese sowel as biologiese faktore bepaal die biobeskikbaarheid van chemiese stowwe vir organismes wat in die grond lewe. Hierdie oorsig van onlangse literatuur oor die gebruik van die konsep van biobeskikbaarheid in die ekotoksikologie, lei tot die slotsom dat hierdie konsep dikwels ongekwalifiseerd en teenstrydig gebruik word omdat dit verskillende dinge vir verskillende outeurs beteken. ’n Heldere begrip van die konsep is van wesenlike belang vir die interpretasie van toksisiteitstoetse, omgewingsmonitering en risiko-assessering van omgewingskontaminante. Dit is ook nodig vir die stel van grondkwaliteitskriteria omdat kennis van die werklike blootstelling van organismes nodig is, en nie bloot van die totale hoeveelheid van die chemiese stof in die omgewing nie. Die doel van hierdie bydrae is om die konsep duidelik toe te lig ter wille van konsekwente en verantwoordbare aanwending daarvan. Afgesien van die definiëring of omskrywing van biobeskikbaarheid, word die probleme rondom die vergelykbaarheid van toksisiteitsgegewens tussen gronde en tussen spesies bespreek. Verder word die potensiële rol wat biomerkers in grondorganismes kan speel om biobeskikbaarheid te bepaal, aangeroer. In ’n poging om eenvormige kriteria of standaarde vir omgewingskwaliteit neer te lê, sal rekening gehou moet word met beide biotiese sowel as abiotiese eienskappe wat die biobeskikbaarheid van chemiese stowwe vir grondorganismes bepaal. Dit vereis dus ’n dinamiese benadering wat beide fisies-chemies gedrewe desorpsieprosesse en fisiologies gedrewe opnameprosesse, sowel as ’n verskeidenheid ander biologiese faktore, in ag moet neem. Die bevinding is dat biobeskikbaarheid kwalitatief geïnterpreteer moet word en dat die opnamekoers van ’n chemiese stof moontlik as maatstaf van biobeskikbaarheid kan dien. Die ontwikkeling van gestandaardiseerde protokolle vir blootstelling van geselekteerde spesies en meting van biologiese response met behulp van biomerkers kan risiko-assessering verfyn en ’n stap verder neem.Publishers' Versio

Sellulêre biomerkerresponse as maatstaf van gevoeligheid van klipmossels (Mollusca) vir kadmiumbesoedeling

The original publication is available at http://www.satnt.ac.za/Die bioakkumulasie van kadmium in tussengetyspesies kan stres veroorsaak wat op sellulêre vlak meetbaar is. Verskeie klipmosselspesies kom volop op rotse aan die Suid-Afrikaanse kuslyn voor en kan moontlik vir ekotokiskologiese monitering gebruik word. Die oogmerk van die studie was om sensitiwiteitsdata te verkry wat kan bydrae tot die kies van ’n geskikte spesie vir monitering en die uiteindelike daarstelling van ’n model vir spesie sensitiwiteitsverspreiding (SSV) wat ’n biomerkerrespons as eindpunt gebruik. Die klipmossels Cymbula oculus, Scutellastra longicosta, Cymbula granatina en Scutellastra granularis en watermonsters is in Valsbaai versamel. Analises van kadmium in water en biologiese monsters is met behulp van atoomabsorpsiespektrofotometrie uitgevoer. Blootstellings van organismes aan subletale vlakke van kadmium is uitgevoer in statiese vloeitenks oor ’n periode van drie dae. Daar was ’n matige toename in liggaamskonsentrasies van kadmium oor tyd. Resultate wat by drie blootstellingskonsentrasies gekry is, het geen betekenisvolle verskille in metaalkonsentrasies tussen die verskillende C. oculus monsters uitgewys nie. Betekenisvolle verskille tussen die kontrole en die blootstellingsgroepe vir elke individuele blootstellingstyd is vir die spesie verkry behalwe tussen die kontrole en die 1mg/L CdCl2 blootstellingsgroep na 24 en 72 uur van blootstelling. Cd liggaamskonsentrasies (sagteweefsel) het tussen 4.56 en 21.41μg/g (nat massa) gevarieer. Gemiddelde Cd konsentrasies in sagteweefsel van S. longicosta was aansienlik laer (variërend tussen 1.18 en 19.58 μg/g Cd ) as in weefsel van C. oculus. Die kontrolegroep van hierdie spesie het betekenisvol verskil van die 0.8 en 1 mg/L CdCl2 blootstellings na 48 en 72 uur. Gemiddelde Cd liggaamskonsentrasies in S. granularis was die hoogste van al die blootgestelde spesies en het ’n vlak van 148 μg/g Cd by die hoogste blootstellingskonsentrasie bereik en het betekenisvol verskil van die ander monsters se gemiddeldes met die uitsondering van die 0.8 mg/ L CdCl2 blootstellingsgroep by 72 uur en die 1 mg/L CdCl2 groep by 24 uur. Betekenisvolle verskille is ook verkry vir die liggaamskonsentrasies van Cd van C. granatina tussen die drie verskillende blootstellingskonsentrasies en drie blootstellingstye. Integriteit van lisosoommembrane is bepaal met behulp van die neutraalrooi retensiemetode. Drie van die vier spesies het ’n betekenisvolle afname in retentsietye getoon met ’n toename in Cd konsentrasie. Tussenspesieverskille in gevoelighede vir omgewingsrelevante kadmiumkonsentrasies is deur die biomerkerresponse uitgewys. Gebaseer op die verlaging in NRR tye, is die volgorde van relatiewe gevoeligheid vir kadmium as volg: S. granularis > C. oculus> S. longicosta.> C. granatina.Publishers' Versio

Correction of diffraction effects in confocal raman microspectroscopy

A mathematical approach developed to correct depth profiles of wet-chemically modified polymer films obtained by confocal Raman microscopy is presented which takes into account scattered contributions originated from a diffraction-limited laser focal volume. It is demonstrated that the problem can be described using a linear Fredholm integral equation of the first kind which correlates apparent and true Raman intensities with the depth resolution curve of the instrument. The calculations of the corrected depth profiles show that considerable differences between apparent and corrected depth profiles exist at the surface, especially when profiles with strong concentration gradients are dealt with or an instrument with poor depth resolution is used. Degrees of modification at the surface obtained by calculation of the corrected depth profiles are compared with those measured by FTIR-ATR and show an excellent concordance.</p

A new model for deflagration fronts in reactive fluids

We present a new way of modeling deflagration fronts in reactive fluids, the main emphasis being on turbulent thermonuclear deflagration fronts in white dwarfs undergoing a Type Ia supernova explosion. Our approach is based on a level set method which treats the front as a mathematical discontinuity and allows full coupling between the front geometry and the flow field. With only minor modifications, this method can also be applied to describe contact discontinuities. Two different implementations are described and their physically correct behaviour for simple testcases is shown. First results of the method applied to the concrete problems of Type Ia supernovae and chemical hydrogen combustion are briefly discussed; a more extensive analysis of our astrophysical simulations is given in (Reinecke et al. 1998, MPA Green Report 1122b).Comment: 11 pages, 13 figures, accepted by A&A, corrected and extended according to referee's comment

How are practices made to vary? Managing practice adaptation in a multinational corporation

Research has shown that management practices are adapted and ‘made to fit’ the specific context into which they are adopted. Less attention has been paid to how organizations anticipate and purposefully influence the adaptation process. How do organizations manage the tension between allowing local adaptation of a management practice and retaining control over the practice? By studying the adaptation of a specialized quality management practice – ACE (Achieving Competitive Excellence) – in a multinational corporation in the aerospace industry, we examine how the organization manages the adaptation process at the corporate and subsidiary levels. We identified three strategies through which an organization balances the tension between standardization and variation – preserving the ‘core’ practice while allowing local adaptation at the subsidiary level: creating and certifying progressive achievement levels; setting discretionary and mandatory adaptation parameters; and differentially adapting to context-specific and systemic misfits. While previous studies have shown how and why practices vary as they diffuse, we show how practices may diffuse because they are engineered to vary for allowing a better fit with diverse contextual specificities

Detailed Spectral Modeling of a 3-D Pulsating Reverse Detonation Model: Too Much Nickel

We calculate detailed NLTE synthetic spectra of a Pulsating Reverse Detonation (PRD) model, a novel explosion mechanism for Type Ia supernovae. While the hydro models are calculated in 3-D, the spectra use an angle averaged hydro model and thus some of the 3-D details are lost, but the overall average should be a good representation of the average observed spectra. We study the model at 3 epochs: maximum light, seven days prior to maximum light, and 5 days after maximum light. At maximum the defining Si II feature is prominent, but there is also a prominent C II feature, not usually observed in normal SNe Ia near maximum. We compare to the early spectrum of SN 2006D which did show a prominent C II feature, but the fit to the observations is not compelling. Finally we compare to the post-maximum UV+optical spectrum of SN 1992A. With the broad spectral coverage it is clear that the iron-peak elements on the outside of the model push too much flux to the red and thus the particular PRD realizations studied would be intrinsically far redder than observed SNe Ia. We briefly discuss variations that could improve future PRD models.Comment: 15 pages, 4 figures, submitted to Ap

Can Deflagration-Detonation-Transitions occur in Type Ia Supernovae?

The mechanism for deflagration-detonation-transition (DDT) by turbulent preconditioning, suggested to explain the possible occurrence of delayed detonations in Type Ia supernova explosions, is argued to be conceptually inconsistent. It relies crucially on diffusive heat losses of the burned material on macroscopic scales. Regardless of the amplitude of turbulent velocity fluctuations, the typical gradient scale for temperature fluctuations is shown to be the laminar flame width or smaller, rather than the factor of thousand more required for a DDT. Furthermore, thermonuclear flames cannot be fully quenched in regions much larger than the laminar flame width as a consequence of their simple ``chemistry''. Possible alternative explosion scenarios are briefly discussed.Comment: 8 pages, uses aastex; added references. Accepted by ApJ Letter

Direct Numerical Simulations of Type Ia Supernovae Flames II: The Rayleigh-Taylor Instability

A Type Ia supernova explosion likely begins as a nuclear runaway near the center of a carbon-oxygen white dwarf. The outward propagating flame is unstable to the Landau-Darrieus, Rayleigh-Taylor, and Kelvin-Helmholtz instabilities, which serve to accelerate it to a large fraction of the speed of sound. We investigate the Rayleigh-Taylor unstable flame at the transition from the flamelet regime to the distributed-burning regime, around densities of $10^7$ g/cc, through detailed, fully resolved simulations. A low Mach number, adaptive mesh hydrodynamics code is used to achieve the necessary resolution and long time scales. As the density is varied, we see a fundamental change in the character of the burning--at the low end of the density range the Rayleigh-Taylor instability dominates the burning, whereas at the high end the burning suppresses the instability. In all cases, significant acceleration of the flame is observed, limited only by the size of the domain we are able to study. We discuss the implications of these results on the potential for a deflagration to detonation transition.Comment: submitted to ApJ, some figures degraded due to size constraint

WOODLAND POND SALAMANDER ABUNDANCE IN RELATION TO FOREST MANAGEMENT AND ENVIRONMENTAL CONDITIONS IN NORTHERN WISCONSIN

Woodland ponds are important landscape features that help sustain populations of amphibians that require this aquatic habitat for successful reproduction. Species abundance patterns often reflect site-specific differences in hydrology, physical characteristics, and surrounding vegetation. Large-scale processes such as changing land cover and environmental conditions are other potential drivers influencing amphibian populations in the Upper Midwest, but little information exists on the combined effects of these factors. We used Blue-spotted (Ambystoma laterale Hallowell) and Spotted Salamander (A. maculatum Shaw) monitoring data collected at the same woodland ponds thirteen years apart to determine if changing environmental conditions and vegetation cover in surrounding landscapes influenced salamander movement phenology and abundance. Four woodland ponds in northern Wisconsin were sampled for salamanders in April 1992-1994 and 2005-2007. While Bluespotted Salamanders were more abundant than Spotted Salamanders in all ponds, there was no change in the numbers of either species over the years. However, peak numbers of Blue-spotted Salamanders occurred 11.7 days earlier (range: 9-14 days) in the 2000s compared to the 1990s; Spotted Salamanders occurred 9.5 days earlier (range: 3 - 13 days). Air and water temperatures (April 13- 24) increased, on average, 4.8 oC and 3.7 oC, respectively, between the decades regardless of pond. There were no discernible changes in canopy openness in surrounding forests between decades that would have warmed the water sooner (i.e., more light penetration). Our finding that salamander breeding phenology can vary by roughly 10 days in Wisconsin contributes to growing evidence that amphibian populations have responded to changing climate conditions by shifting life-cycle events. Managers can use this information to adjust monitoring programs and forest management activities in the surrounding landscape to avoid vulnerable amphibian movement periods. Considering direct and indirect stressors such as changing habitat and environmental conditions simultaneously to better understand trends in space and time can help improve monitoring programs for this taxa, which is at major risk of continued declines