Advanced immunostaining approaches to study early male germ cell development

Mammalian male germ cell development takes place in the testis under the influence of a variety of somatic cells and an incompletely defined paracrine and endocrine influences. Since it is not recapitulated well in vitro, researchers studying spermatogenesis often manipulate the germline by creating transgenic or knockout mice or by administering pharmaceutical agonists/antagonists or inhibitors. The effects of these types of manipulations on germline development can often be determined following microscopic imaging, both of stained and immunostained testis sections. Here, we describe approaches for microscopic analysis of the developing male germline, provide detailed protocols for a variety of immunostaining approaches, and discuss transgenic fluorescent reporter lines for studying the early stages of spermatogenesis

Measurement of boundary layer transition on oscillating airfoil using cntTSP in low-speed wind tunnel

cntTSP is a flow visualization technique that combines temperature-sensitive paint (TSP) and carbon nanotube (CNT). TSP is a sprayable temperature sensor, and CNT is used as a thin inner electric heater for the TSP. The cntTSP technique allows the visualization of the movement of the boundary layer transition on a moving airfoil. In this study, cntTSP was applied to a pitch-oscillating airfoil in a low-speed wind tunnel. Three methods were compared as methods for detecting the boundary layer transition position. The transition position was successfully evaluated using a heat transfer coefficient that was calculated by the surface temperature. Moreover, at a high-reduced frequency, the detected transition positions were different between the pitching-up and pitching-down procedure. Moreover, we observed a hysteresis loop of the transition position