Influencia de componentes dietarios en el téjido óseo : sonrisas sanas

Fil: Gorustovich, Alejandro A. Universidad de Buenos Aires. Facultad de Odonotología; ArgentinaFil: Steimetz, Tammy. Universidad de Buenos Aires. Facultad de Odontología; ArgentinaFil: Nielsen, Forrest H. Universidad de Buenos Aires. Facultad de Odontología; ArgentinaFil: Guglielmotti, María Beatriz. Universidad de Buenos Aires.Facultad de Odontología; ArgentinaDada la importancia nutricional de ciertos elementos minerales, como por ejemplo\nfósforo, sodio, potasio, magnesio, cobre, zinc, silicio y boro, sobre la formación y\nmantenimiento de los tejidos cartilaginoso y óseo, investigadores de la UBA, juntamente\ncon el Departamento de Agricultura de los Estados Unidos de Norteamérica (USDA), han\nestudiado el efecto de la deficiencia nutricional de boro sobre la reparación, modelado y\nremodelado del tejido óseo

Influencia de componentes dietarios en el téjido óseo : sonrisas sanas

Fil: Gorustovich, Alejandro A. Universidad de Buenos Aires. Facultad de Odonotología; ArgentinaFil: Steimetz, Tammy. Universidad de Buenos Aires. Facultad de Odontología; ArgentinaFil: Nielsen, Forrest H. Universidad de Buenos Aires. Facultad de Odontología; ArgentinaFil: Guglielmotti, María Beatriz. Universidad de Buenos Aires.Facultad de Odontología; ArgentinaDada la importancia nutricional de ciertos elementos minerales, como por ejemplo\nfósforo, sodio, potasio, magnesio, cobre, zinc, silicio y boro, sobre la formación y\nmantenimiento de los tejidos cartilaginoso y óseo, investigadores de la UBA, juntamente\ncon el Departamento de Agricultura de los Estados Unidos de Norteamérica (USDA), han\nestudiado el efecto de la deficiencia nutricional de boro sobre la reparación, modelado y\nremodelado del tejido óseo

Variant anatomy of the coronary arteries and their branches. Mathematical calculations type blood supply heart based morphometric parameters

Introduction: Despite the long history of the study of the heart as the central organ of cardio - vascular system, the problems associated with the pathology of coronary arteries (CA) and their branches do not lose their relevance to this day. For many years, the focus was research CA, in various pathologies, the results of which do not provide a complete picture of architectonic features and morphometric characteristics. Equally important is to determine the blood supply of the heart (TBSH). The classical definition of TBSH describes exclusively anatomical features architectonic vessels and does not give the amount of blood flowing through the vessels and the blood supply to the area. "White Spots" to explore issues underscore the urgency of research topics in theoretical and clinical relationships. Purpose and objectives: To determine the particular variant anatomy and subepicardial vascular branching CA, as well as mathematically calculated based on the type of blood supply to the morphometric data. Subjects: 58 human heart preparations of both sexes. Methods: Makrodissection, mikrodissection, morphometry, the statistical method, mathematical modeling. Results: During the study it was found that the left coronary artery (LCA) has three options architectonics: typical - bifurcation, with the division into two branches (anterior descending branch (ADB) and the circumflex branch (CB) - 75%), trifurcation (ADB, CB and left marginal branch (LMB) - 23%), kvadrifurkatsiya (ADB, CB, LMB and branch cone (BC) - 2%). ADB for the anterior interventricular sulcus also has 3 options architectonics: 1 - reaches apex of the heart - 55 % 2 - disappears between the middle and lower third of the anterior interventricular sulcus - 15 % 3 - goes around the top of the heart and extends into the lower third of the posterior interventricular sulcus - 30 %. As well, we have developed an algorithm of actions and calculations, allows us to calculate TCS from a mathematical point of view, given the above mentioned morphometric parameters. Conclusion: The method proposed for the detenuination o f blood supply with the help of mathematical calculations, to determine the TBSH based not only on the anatomical structure as well as on the morphometric parameters of the heart. Data on structural - functional organization and topographic - anatomical relationships VA obtained using modern research techniques, including computer programs, can be used in anatomy - in particular, in addition to the guidelines, as well as in cardiology and cardiac surgery

Evaluation of antibacterial and cytotoxic effects of nano-sized bioactive glass/collagen composites releasing tetracycline hydrochloride

Aims: To evaluate the antibacterial efficacy of silicate bioactive glass nanoparticles/collagen composites functionalized with tetracycline hydrochloride (TCH). Methods and Results: Different concentrations of tetracycline hydrochloride (TCH) were incorporated on silicate bioactive glass nanoparticles/collagen composites by dipping these biomaterials for 48 h at 37°C in a solution of simulated body fluid (SBF) plus 0 05, 0 20 or 0 35 mg ml1 of the antibiotic. TCH release was assessed in double-distilled water at 37°C up to 72 h. The antibacterial activity of the samples has been evaluated in two ways: inhibition zone test and plate count method. The experiments were performed in vitro up to 48 h on four staphylococci strains (Staphylococcus aureus ATCC29213, ATCC25923, ATCC6538P and Staphylococcus epidermidis ATCC12228). The new composites were also tested for cytotoxicity on MG-63 human osteosarcoma cells. The results showed that the incorporation and release of TCH was dependent on the initial concentration of TCH in SBF. The biomaterials also inhibited the Staph. aureus cell growth even though the efficacy was similar for all concentration. On the other hand, no cytotoxic effects were found on osteoblast-like cells, even at the highest concentration. Conclusions: Considering all results, it can be concluded that the new composite acts as a suitable bioactive carrier of TCH and could have potential in the prevention of biomaterial related infections. Significance and Impact of the Study: The results suggest a potential application as wound dressing.Fil: Rivadeneira, Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long". Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long"; Argentina. Universidad Nacional de Salta; ArgentinaFil: Di Virgilio, Ana Laura. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Audisio, Marcela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; ArgentinaFil: Boccaccini, A. R.. University of Erlangen-Nuremberg; AlemaniaFil: Gorustovich Alonso, Alejandro Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long". Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long"; Argentina. Universidad Nacional de Salta; Argentin

Resistance screening against the South American tomato pinworm (Tuta absoluta) in tomato by means of a simple bioessay

Se describe un bioensayo en invernáculo para evaluar poblaciones segregantes de tomate para la resistencia a la polilla del tomate (Tuta absoluta Meyrick = Scrobipalpuloides absoluta Meyrick). Se evaluaron dos cultivares de Lycopersicon esculentum y tres accesiones silvestres del género Lycopersicon. Se infestaron hojas jóvenes completamente expandidas de plantas adultas cultivadas en macetas en invernáculo, con hojas de tomate que llevaban larvas neonatas de polilla. El grado de infestación se evaluó a los 7, 14 y 21 días después de iniciado el bioensayo. No se encontraron diferencias significativas entre plantas dentro de genotipos. Se encontraron diferencias altamente significativas entre genotipos a los 21 días. La heredabilidad en sentido amplio del grado de infestación a los 21 días fue de 0,95 lo que demuestra su alta repetibilidad. Se concluye que el bioensayo es simple, altamente repetible y permite discriminar entre plantas resistentes y susceptibles en grandes poblaciones segregantes en el invernáculo y en el campo.A greenhouse bioessay is described for screening resistance against the South American tomato pinworm (Tuta absoluta Meyrick = Scrobipalpuloides absoluta Meyrick) in large tomato populations. Two L. esculentum cultivars and three wild Lycopersicon accessions were evaluated. Young fully expanded leaves of adult plants grown in pots were infested using young tomato leaves carrying neonate larvae. The infestation rate was evaluated 7, 14 and 21 days after the infestation. No differences were found between plants within genotypes. Highly significant differences between genotypes were found twenty one days after the infestation date. The broadsense heritability of the infestation rate at twenty one days was 0.95, indicating the high reproducibility of the bioessay. The current results show that the bioessay is simple, highly reproducible and it allows the discrimination between resistant and susceptible plants in large segregating populations, both in the greenhouse and in the field.Facultad de Ciencias Agrarias y Forestale

Resistance screening against the South American tomato pinworm (Tuta absoluta) in tomato by means of a simple bioessay

Se describe un bioensayo en invernáculo para evaluar poblaciones segregantes de tomate para la resistencia a la polilla del tomate (Tuta absoluta Meyrick = Scrobipalpuloides absoluta Meyrick). Se evaluaron dos cultivares de Lycopersicon esculentum y tres accesiones silvestres del género Lycopersicon. Se infestaron hojas jóvenes completamente expandidas de plantas adultas cultivadas en macetas en invernáculo, con hojas de tomate que llevaban larvas neonatas de polilla. El grado de infestación se evaluó a los 7, 14 y 21 días después de iniciado el bioensayo. No se encontraron diferencias significativas entre plantas dentro de genotipos. Se encontraron diferencias altamente significativas entre genotipos a los 21 días. La heredabilidad en sentido amplio del grado de infestación a los 21 días fue de 0,95 lo que demuestra su alta repetibilidad. Se concluye que el bioensayo es simple, altamente repetible y permite discriminar entre plantas resistentes y susceptibles en grandes poblaciones segregantes en el invernáculo y en el campo.A greenhouse bioessay is described for screening resistance against the South American tomato pinworm (Tuta absoluta Meyrick = Scrobipalpuloides absoluta Meyrick) in large tomato populations. Two L. esculentum cultivars and three wild Lycopersicon accessions were evaluated. Young fully expanded leaves of adult plants grown in pots were infested using young tomato leaves carrying neonate larvae. The infestation rate was evaluated 7, 14 and 21 days after the infestation. No differences were found between plants within genotypes. Highly significant differences between genotypes were found twenty one days after the infestation date. The broadsense heritability of the infestation rate at twenty one days was 0.95, indicating the high reproducibility of the bioessay. The current results show that the bioessay is simple, highly reproducible and it allows the discrimination between resistant and susceptible plants in large segregating populations, both in the greenhouse and in the field.Facultad de Ciencias Agrarias y Forestale

2- tridecanone content and resistence to Tuta absoluta Meyrick and Tetranychus urticae Koch in tomato

La polilla del tomate y la arañuela roja constituyen dos plagas importantes del tomate cultivado (Lycopersicon esculentum ) en la Argentina. La accesión PI 134417 de Lycopersicon hirsutum f. glabratum es resistente a ambos artrópodos. La resistencia de las especies silvestres de tomate fue asociada con la presencia de metabolitos secundarios como la 2-tridecanona, 2-undecanona, alfatomatina, etc. En el presente trabajo se cuantificó por colorimetría la concentración de 2-tridecanona y se evaluó el grado de infestación de la polilla del tomate y de la arañuela roja en los padres susceptible, UCO PLATA INTA ( Lycopersicon esculentum) y resistente, PI 134417 (L. hirsutum f. glabratum), sus F1 y F2 cultivados en invernadero y a campo. La concentración de 2-tridecanona en PI 134417 fue treinta y seis veces mayor que en el padre susceptible en el campo; en invernadero la diferencia fue de quince veces. La alta concetración de 2-tridecanona estaría gobernada por varios genes de efecto recesivo. El grado de infestación medio de la polilla del tomate fue significativamente superior en el cv. UCO PLATA que en PI 134417 en ambos ambientes. Las medias de la F1 y F2 sugieren un efecto promedio génico aditivo para este carácter. La heredabilidad amplia estimada en invernadero fue 70% y en el campo del 56%. PI 134417 resultó altamente resistente a la arañuela roja en ambos ambientes. Las medias de F1 y F2 sugieren que la resistencia a la arañuela roja sería un carácter recesivo. No se detectó correlación entre la concentración de 2-tridecanona y el grado de infestación de la arañuela roja, por lo que la 2-tridecanona no estaría involucrada en la resistencia a este ácaro. Se detectó una correlación negativa significativa entre la concentración de 2-tridecanona y el grado de infestación de la polilla del tomate en invernadero pero no en campo. La 2-tridecanona podría explicar sólo parcialmente la resistencia a la polilla en invernadero; en el campo se podría desencadenar otro mecanismo de resistencia independiente de la 2-tridecanona.The South American tomato pinworm and the two spotted spider mite are two important pests of the tomato crop in Argentina. The wild accession PI 134417 of Lycopersicon hirsutum f. glabratum is resistant to both pests. The resistance to insects in the wild tomato accessions has been associated to the presence of secondary metabolites as 2-tridecanone, 2-undecanone, alpha-tomatine, etc. In this work the concentration of 2-tridecanone was colorimetrically quantified and the tomato pinworm and two spotted spider mite degrees of infestation were evaluated in the susceptible cultivar Uco Plata INTA (L. esculentum), the resistant accession PI 134417 (L. hirsutum f. glabratum) and their F1 and F2, grown in the greenhouse and in the field. In the field essay the 2-tridecanone concentration in PI 134417 was 36 times higher than in Uco Plata; in the greenhouse it was 15 times higher. The high 2-tridecanone concentration would be controlled by several genes of recessive effect. The mean infestation degree of the tomato pinworm was significantly higher in the cv. Uco Plata than in PI 134417 in both environments. The F1 and F2 means suggest a mean genic additive effect for this trait. The estimated broad heritability was 70% in the greenhouse and 56 % in the field. PI 134417 was highly resistant to the two spotted spider mite in both environments. The F1 and F2 means suggest that the resistance to this pest would be a recessive trait. No correlation was found between the 2-tridecanone concentration and the two spotted spider mite degree of infestation, so the 2- tridecanone would not be involved in the resistance. A significative negative correlation was found between the 2-tridecanone concentration and the tomato pinworm degree of infestation in the greenhouse but not in the field. The 2-tridecanone could account only partially for the tomato pinworm resistance in the greenhouse. In the field another resistance mechanism could be involved independently of the 2-tridecanone.Facultad de Ciencias Agrarias y Forestale

45S5 Bioglass® concentrations modulate the release of vancomycin hydrochloride from gelatin-starch films: evaluation of antibacterial and cytotoxic effects

The aim of this work was to evaluate the release profile of vancomycinhydrochloride (VC), as well as the degradation, in vitro antistaphylococcal effect and cytotoxicity in MG-63 osteoblast-like cells of gelatin–starch (GS) films added with different concentrations of microparticles of the bioactive glass 45S5 (m-BG). The biomaterials were obtained through the gel-casting method. Four different composites were prepared at four different weight percentages of m-BG: 0, 5, 10, and 15 %. Glutaraldehyde 0.25 wt% (GA) was used as the cross-linker. The composites were characterized by scanning electron microscopy and the in vitro degradation of the filmswas studied bymeasuring thewater uptake and weight loss. The drug release kinetics was quantified spectrophotometrically. The inhibition zone test and the plate count method were used to evaluate the antibacterial activity of the samples. Three staphylococcus strains were evaluated: Staphylococcus aureus ATCC6538, S. aureus ATCC29213, and Staphylococcus epidermidis ATCC12228. Cytotoxicity effects were evaluatedthrough the MTT assay.The addition of m-BG to GS films showedno effects on the amount of water uptake, but led to an increase in the weight loss over time, even with m-BG content. The release rate of VC was also affected by the increasing concentration of m-BG in the composite films.However, the antibacterial effects of the composites were not improved by this modulation. All composites strongly inhibited staphylococcal cells with similar strength. On the other hand, liquid extracts from the composites resultedin cytotoxic effects onMG-63 osteoblastlike cells due to the presence of GA, but not to the concentration of VC or m-BG.Facultad de Ciencias Exacta