Economía social y solidaria en la ciudad de Neuquén : las organizaciones y sus prácticas post-crisis del 2001

La crisis de 2001 trajo aparejada una reconfiguración social, política y económica que marcó un quiebre en la sociedad argentina. En la ciudad de Neuquén, fue el contexto de surgimiento de múltiples organizaciones que llevaron adelante prácticas asociadas a la Economía Social y Solidaria (ESS). En el presente trabajo se propone realizar una descripción de las organizaciones que tienen prácticas de Economía Social y Solidaria en la Ciudad de Neuquén, y que nacieron en el período 2001-2015. Algunos rasgos de la provincia de Neuquén y su ciudad capital se tornan fundamentales para conocer en qué marco se desenvolvió histórica y actualmente la ESS. Se realiza primero una breve descripción, de algunas características espaciales, económicas, sociales y políticas que se consideran distintivas, a modo de contextualización de la presente investigación.Fil: Fachinetti Guillén, Micaela A.. Universidad Nacional de Cuyo. Facultad de Ciencias Económicas

Phosphorylation of CENP-A on serine 7 does not control centromere function

CENP-A is the histone H3 variant necessary to specify the location of all eukaryotic centromeres via its CENP-A targeting domain and either one of its terminal regions. In humans, several post-translational modifications occur on CENP-A, but their role in centromere function remains controversial. One of these modifications of CENP-A, phosphorylation on serine 7, has been proposed to control centromere assembly and function. Here, using gene targeting at both endogenous CENP-A alleles and gene replacement in human cells, we demonstrate that a CENP-A variant that cannot be phosphorylated at serine 7 maintains correct CENP-C recruitment, faithful chromosome segregation and long-term cell viability. Thus, we conclude that phosphorylation of CENP-A on serine 7 is dispensable to maintain correct centromere dynamics and function

Economía social y solidaria

El presente trabajo tiene como objetivo general explorar la relación entre las organizaciones de Economía Social y Solidaria (ESS) y el desarrollo económico de la ciudad de Neuquén, Argentina en la actualidad. Los objetivos específicos, por su parte, son: identificar las organizaciones de ESS que existen en la Ciudad de Neuquén; describir las actividades económicas que realizan las organizaciones de ESS y analizar la contribución de las actividades económicas de estas organizaciones a un mayor grado de desarrollo económico en dicha localidad.Fil: Fachinetti Guillén, Micaela A.. Universidad Nacional de Cuyo. Facultad de Ciencias Económicas

CENP-A Is Dispensable for Mitotic Centromere Function after Initial Centromere/Kinetochore Assembly

Human centromeres are defined by chromatin containing the histone H3 variant CENP-A assembled onto repetitive alphoid DNA sequences. By inducing rapid, complete degradation of endogenous CENP-A, we now demonstrate that once the first steps of centromere assembly have been completed in G1/S, continued CENP-A binding is not required for maintaining kinetochore attachment to centromeres or for centromere function in the next mitosis. Degradation of CENP-A prior to kinetochore assembly is found to block deposition of CENP-C and CENP-N, but not CENP-T, thereby producing defective kinetochores and failure of chromosome segregation. Without the continuing presence of CENP-A, CENP-B binding to alphoid DNA sequences becomes essential to preserve anchoring of CENP-C and the kinetochore to each centromere. Thus, there is a reciprocal interdependency of CENP-A chromatin and the underlying repetitive centromere DNA sequences bound by CENP-B in the maintenance of human chromosome segregation

Skin Delivery And In Vitro Biological Evaluation Of Trans-resveratrol-loaded Solid Lipid Nanoparticles For Skin Disorder Therapies.

The aim of this study was to evaluate the skin delivery and in vitro biological activity of trans-resveratrol (RES)-loaded solid lipid nanoparticles (SLNs). The SLNs were composed of stearic acid, poloxamer 407, soy phosphatidylcholine (SPC), an aqueous phase and 0.1% RES. The particle size, polydispersity index (PdI) and zeta potential were analyzed by dynamic light scattering (DLS). The SLNs were analyzed by scanning electron microscopy (SEM-FEG) and differential scanning calorimetry (DSC). In vitro RES-SLN skin permeation/retention assays were conducted, and their tyrosinase inhibitory activity was evaluated. An MTT reduction assay was performed on HaCat keratinocytes to determine in vitro cytotoxicity. The formulations had average diameter lower than 200 nm, the addition of SPC promoted increases in PdI in the RES-SLNs, but decreases PdI in the RES-free SLNs and the formulations exhibited zeta potentials smaller than -3 mV. The DSC analysis of the SLNs showed no endothermic peak attributable to RES. Microscopic analysis suggests that the materials formed had nanometric size distribution. Up to 45% of the RES permeated through the skin after 24 h. The RES-loaded SLNs were more effective than kojic acid at inhibiting tyrosinase and proved to be non-toxic in HaCat keratinocytes. The results suggest that the investigated RES-loaded SLNs have potential use in skin disorder therapies.2

Skin delivery and in vitro biological evaluation of trans-resveratrol-loaded solid lipid nanoparticles for skin disorder therapies

The aim of this study was to evaluate the skin delivery and in vitro biological activity of trans-resveratrol (RES)-loaded solid lipid nanoparticles (SLNs). The SLNs were composed of stearic acid, poloxamer 407, soy phosphatidylcholine (SPC), an aqueous phase and 0.1% RES. The particle size, polydispersity index (PdI) and zeta potential were analyzed by dynamic light scattering (DLS). The SLNs were analyzed by scanning electron microscopy (SEM-FEG) and differential scanning calorimetry (DSC). In vitro RES-SLN skin permeation/retention assays were conducted, and their tyrosinase inhibitory activity was evaluated. An MTT reduction assay was performed on HaCat keratinocytes to determine in vitro cytotoxicity. The formulations had average diameter lower than 200 nm, the addition of SPC promoted increases in PdI in the RES-SLNs, but decreases PdI in the RES-free SLNs and the formulations exhibited zeta potentials smaller than −3 mV. The DSC analysis of the SLNs showed no endothermic peak attributable to RES. Microscopic analysis suggests that the materials formed had nanometric size distribution. Up to 45% of the RES permeated through the skin after 24 h. The RES-loaded SLNs were more effective than kojic acid at inhibiting tyrosinase and proved to be non-toxic in HaCat keratinocytes. The results suggest that the investigated RES-loaded SLNs have potential use in skin disorder therapies21CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPsem informaçãosem informação2011/16888-5; 2012/19568-4; 2013/21500-

O trabalho pedagógico para alunos público-alvo da educação especial: investigando a inclusão na educação infantil

A atuação do professor da Educação Infantil com alunos público-alvo da Educação Especial tem assumido relevância no contexto educacional inclusivo, uma vez que a mediação desse professor, bem como a adoção de metodologias compatíveis com tal perspectiva, nessa etapa educacional, é preponderante para a garantia de acesso, permanência e participação desses alunos na escola, desde o início de seu processo de escolarização. Subsidiado por tais ideias, o presente trabalho teve por objetivo investigar como se dá o trabalho pedagógico desse professor, em classe regular, na Educação Infantil, para alunos público-alvo da Educação Especial, com vistas à apropriação do currículo, por parte desse público. Foram realizadas observações em salas de aula de quatro professoras que atuavam em classes de educação infantil, que contavam com alunos público-alvo da Educação Especial matriculados, de quatro escolas de dois municípios do interior paulista. Os resultados desse estudo demonstraram de que forma esses alunos foram incluídos nas atividades desenvolvidas e de que modo as professoras utilizavam sua prática docente para atender a todos os alunos e, particularmente, aos que se encontravam caracterizados como público-alvo da Educação Especial

Versatile Coordination of Cyclopentadienyl-Arene Ligands and Its Role in Titanium-Catalyzed Ethylene Trimerization

Cationic titanium(IV) complexes with ansa-(η5-cyclopentadienyl,η6-arene) ligands were synthesized and characterized by X-ray crystallography. The strength of the metal-arene interaction in these systems was studied by variable-temperature NMR spectroscopy. Complexes with a C1 bridge between the cyclopentadienyl and arene moieties feature hemilabile coordination behavior of the ligand and consequently are active ethylene trimerization catalysts. Reaction of the titanium(IV) dimethyl cations with CO results in conversion to the analogous cationic titanium(II) dicarbonyl species. Metal-to-ligand backdonation in these formally low-valent complexes gives rise to a strongly bonded, partially reduced arene moiety. In contrast to the η6-arene coordination mode observed for titanium, the more electron-rich vanadium(V) cations [cyclopentadienyl-arene]V(NiPr2)(NC6H4-4-Me)+ feature η1-arene binding, as determined by a crystallographic study. The three different metal-arene coordination modes that we experimentally observed model intermediates in the cycle for titanium-catalyzed ethylene trimerization. The nature of the metal-arene interaction in these systems was studied by DFT calculations.