Influence of Rice Production on the Quality of Water in Tailwater Collection Reservoirs

Since maintaining high water quality standards in the state remains a high priority, monitoring for pesticides in water sources must continue. Determining the type, concentration, and characteristics of any pesticides present in water supplies are essential to the overall assessment of water quality. Five independent locations, implementing contained water management systems and recyclable water, were monitored in 1995 and 1996. Irrigation, runoff, and pond water samples were collected every 10 to 14 days between permanent flood establishment and draining. Water samples were transported to the laboratory and extracted for 16 pesticides using solid phase extraction (SPE) techniques. Quantification and confirmation of pesticide residues were obta1ned by HPLC and GC/MS analysis. The lower limit of quantitation for all pesticides was between 1.0 -1.3 pg L-1 in water. Pesticides selected for monitoring were determined after assessing state recommendations and our analytical capabilities. Pesticides included: benomyl, bensulfuron methyl, carbaryl, carbofuran, 2,4-D, fenoxaprop ethyl, propiconazole, malathion, MCPA, methyl parathion, molinate, pendimethalin, propanil, iprodione, quinclorac, triclopyr, and thiobencarb. Since each field location was independently managed, individual results are site specific. 2,4-D, benomyl, molinate, propanil, quinclorac, thiobencarb, and pendimethalin were the pesticides actually applied during the seasons. These pesticides were detected, usually at trace levels, in tailwaters shortly after application but did not appear to buildup in the reservoirs. Quinclorac residues in the tailwaters were more persistent (up to 8 weeks) than the other detected compounds (less than 2 weeks)

The Transesterification of Hickory Nuts into Biodiesel Fuel

• Biodiesel fuels can be produced from a fat and an alcohol via transesterification • Hickory nuts have a high fat content (~60 % by weight) • Can nuts from shagbark hickory (Carya ovata) be used to produce a biodiesel fuel using existing acidcatalyzed transesterification methods

Tilt bed testing of the subjective horizontal

Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2002.This electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.Includes bibliographical references (leaf 25).Mittelstaedt (1987) suggested that inversion illusions which caused space sickness in astronauts was associated with a net headward bias in the body's gravireceptor organs, which could be measured on Earth using a tilting bed. Mittelstaedt showed that when individual subjects were asked to repeatedly position themselves to the gravireceptive subjective horizontal, individuals showed a small (<5 deg.) but consistent head up or head down bias that remained stable when retested weeks, months or even years later. A correlation with inversion illusion was noted in a small number of astronauts. The purpose of the present project was 1) to construct a new bed of slightly different design and 2) to verify Mittelstaedt's findings using a different subject population. Nine subjects each lay on their left side with their head immbobilized using a bite bar. They positioned the bed (and themselves) at the subjective horizontal ten successive times starting from standardized initial tilt angles which ranged from +/- 10 degrees. Tests were then repeated on right side. Four subjects returned a day later for retesting. Results showed that subjects repeatedly positioned themselves at their own subjective gravitational horizontal, which differed from true horizontal by several degrees a head down direction. Results of tests on the left and right side had similar means for most of the nine subjects; however 4 were statistically different. Left and right sides were combined, noting the above error. Mean biases in the subjective horizontal varied from -3.26 to -0.82 degrees head down between subjects, with overall mean -1.65 and s.d. 0.80. There was a statistically significant difference between responses of some subjects. Data from four subjects tested on both days was compared. A statistically significant correlation was not found, perhaps due to the small subject retest population. The differences between Mittelstaedt's data and present results are discussed.by Elizabeth A. Dewell.S.B

Shortage of Rural Veterinarians: Real or Perceived?

Concerns about lack of available jobs in rural veterinary practice (RVP) and ironically difficulties attracting new veterinarians are commonly expressed within the veterinary community. Reports on supply and demand for rural veterinarians have produced conflicting results. A 1990\u27s economic study forecasted a 3.0% increase in available veterinarians in large animal private practice and a 1.7% decrease in demand from 1997 to 2015. However, a later study forecasted a shortage of food supply veterinary medicine (FSVM) veterinarians from 2004 to 2016 ranging from 0.1% (poultry veterinarians) to 6.9% (federal animal health), with mixed food animal practice at 6.6%

Randomized Clinical Trial to Evaluate the Pathogenicity of Bibersteinia trehalosi in Respiratory Disease among Calves

Bibersteinia trehalosi causes respiratory disease in ruminants particularly in wild and domestic sheep. Recently, there has been an increased number of B. trehalosi isolates obtained from diagnostic samples from bovine respiratory disease cases. This study evaluated the role of B. trehalosi in bovine respiratory disease using an intra-tracheal inoculation model in calves. Thirty six cross bred 2–3 month old dairy calves were inoculated intra-tracheally with either leukotoxin negative B. trehalosi, leukotoxin positive B. trehalosi isolate, Mannheimia haemolytica, a combination of leukotoxin negative B. trehalosi and M. haemolytica or negative control. Calves were euthanized and necropsy performed on day 10 of study. B. trehalosi inoculated calves did not have increased lung involvement compared to control calves. Additionally, B. trehalosiwas only cultured once from the lungs of inoculated calves at necropsy. Based on these findings B. trehalosi may not be a primary pathogen of respiratory disease in cattle. Culture of B. trehalosifrom diagnostic submissions should not be immediately identified as a primary cause of respiratory disease

Does the presence of multiple β-lactamases in Gram-negative bacilli impact the results of antimicrobial susceptibility tests and extended-spectrum β-lactamase and carbapenemase confirmation methods?

Objectives: Many multidrug-resistant Gram-negative bacilli (MDR-GNB) harbour multiple β-lactamases. The aim of this study was to assess the impact of multiple β-lactamase carriage on the accuracy of susceptibility tests and extended-spectrum β-lactamase (ESBL) and carbapenemase confirmation methods. Methods: A total of 50 MDR-GNB, of which 29 carried multiple β-lactamases, underwent broth microdilution (BMD) and disk diffusion (DD) testing as well as confirmation tests for ESBLs and carbapenemases. Whole-genome sequencing (WGS) was used for β-lactamase gene identification. Results: Categorical agreement of BMD and DD testing results ranged from 86.5 to 97.7% for 10 β-lactam agents. BMD and DD algorithms for ESBL detection were highly variable; 6 of 8 positive strains carried an ESBL plus a carbapenemase or an AmpC enzyme, which may confound antimicrobial selection. The sensitivity and specificity of the modified carbapenem inactivation method (mCIM) were both 100%, whilst mCIM and EDTA-modified carbapenem inactivation method (eCIM) when used together to differentiate serine from metallo-β-lactamase carriage were both 96%. Xpert® Carba-R results (in vitro diagnostic test) were consistent with WGS results. Predicting phenotypic carbapenem resistance from WGS data overall showed 100% specificity but only 66.7% sensitivity for Enterobacterales isolates that were non-susceptible to imipenem and meropenem. Conclusions: Multiple β-lactamases in MDR-GNB does not impact DD results, the utility of mCIM/eCIM tests, or Xpert Carba-R results. However, ESBL algorithms produced inconsistent results and predicting carbapenem resistance from WGS data was problematic in such strains