A formal methodology to design and deploy dependable wireless sensor networks

Wireless Sensor Networks (WSNs) are being increasingly adopted in critical applications, where verifying the correct operation of sensor nodes is a major concern. Undesired events may undermine the mission of the WSNs. Hence their effects need to be properly assessed before deployment to obtain a good level of expected performance and during the operation in order to avoid dangerous unexpected results. In this paper we propose amethodology that aims at assessing and improving the dependability level of WSNs by means of an event-based formal verification technique. The methodology includes a process to guide designers towards the realization of dependable WSN and a tool ("ADVISES") to simplify its adoption. The tool is applicable to homogeneous WSNs with static routing topologies. It allows to generate automatically formal specifications used to check correctness properties and evaluate dependability metrics at design time and at runtime for WSNs where an acceptable percentage of faults can be defined. During the runtime we can check the behavior of the WSN accordingly to the results obtained at design time and we can detect sudden and unexpected failures, in order to trigger recovery procedures. The effectiveness of the methodology is shown in the context of two case studies, as proof-of-concept, aiming to illustrate how the tool is helpful to drive design choices and to check the correctness properties of the WSN at runtime. Although the method scales up to very large WSNs, the applicability of the methodology maybe compromised by the state space explosion of the reasoning model, which must be faced partitioning large topologies into sub-topologies

Static verification of wireless sensor networks with formal methods

Wireless Sensor Networks (WSNs) are widely recognized as a solution to build monitoring systems, even in critical environments. WSNs, however, are subjected to faults due to several causes (i.e. rain, EMF radiations, vibrations, etc..) and tools and methodologies for the design of dependable WSN-based systems are needed. Formal methods partially meet such needs by assessing the degree of correctness of design models and identifying potential system bottlenecks. The aim of this paper is to define a methodology for the static verification of WSN based systems using a formal language (Event Calculus). In particular we show how the formal specification can be used to verify the design of a WSN in terms of its dependability properties. To this aim, we define a set of correctness specifications that apply to a generic WSN, coupled with specific structural specifications describing the target network topology to evaluate. Finally, after having presented an automatic tool, designed to support the designer, we adopt this methodology to a case study

Big Data in Critical Infrastructures Security Monitoring: Challenges and Opportunities

Critical Infrastructures (CIs), such as smart power grids, transport systems, and financial infrastructures, are more and more vulnerable to cyber threats, due to the adoption of commodity computing facilities. Despite the use of several monitoring tools, recent attacks have proven that current defensive mechanisms for CIs are not effective enough against most advanced threats. In this paper we explore the idea of a framework leveraging multiple data sources to improve protection capabilities of CIs. Challenges and opportunities are discussed along three main research directions: i) use of distinct and heterogeneous data sources, ii) monitoring with adaptive granularity, and iii) attack modeling and runtime combination of multiple data analysis techniques.Comment: EDCC-2014, BIG4CIP-201

The heuristic strategies for assessing wireless sensor network: an event-based formal approach

Wireless Sensor Networks (WSNs) are increasingly being adopted in critical applications. In these networks undesired events may undermine the reliability level; thus their effects need to be properly assessed from the early stages of the development process onwards to minimize the chances of unexpected problems during use. In this paper we propose two heuristic strategies: what-if analysis and robustness checking. They allow to drive designers towards optimal WSN deployment solutions, from the point of view of the connection and data delivery resiliency, exploiting a formal approach based on the event calculus formal language. The heuristics are backed up by a support tool aimed to simplify their adoption by system designers. The tool allows to specify the target WSN in a user-friendly way and it is able to elaborate the two heuristic strategies by means of the event calculus specifications automatically generated. The WSN reliability is assessed computing a set of specific metrics. The effectiveness of the strategies is shown in the context of three case studies

Ultrasound delivery of Surface Enhanced InfraRed Absorption active gold-nanoprobes into fibroblast cells: a biological study via Synchrotron-based InfraRed microanalysis at single cell level

Ultrasound (US) induced transient membrane permeabilisation has emerged as a hugely promising tool for the delivery of exogenous vectors through the cytoplasmic membrane, paving the way to the design of novel anticancer strategies by targeting functional nanomaterials to specific biological sites. An essential step towards this end is the detailed recognition of suitably marked nanoparticles in sonoporated cells and the investigation of the potential related biological effects. By taking advantage of Synchrotron Radiation fourier transform infrared micro-spectroscopy (SR-microftiR) in providing highly sensitive analysis at the single cell level, we studied the internalisation of a nanoprobe within fibroblasts (NIH-3T3) promoted by low-intensity US. To this aim we employed 20 nm gold nanoparticles conjugated with the IR marker 4-aminothiophenol. The significant Surface Enhanced Infrared Absorption provided by the nanoprobes, with an absorbance increase up to two orders of magnitude, allowed us to efficiently recognise their inclusion within cells. Notably, the selective and stable SR- microftiR detection from single cells that have internalised the nanoprobe exhibited clear changes in both shape and intensity of the spectral profile, highlighting the occurrence of biological effects. Flow cytometry, immunofluorescence and murine cytokinesis-block micronucleus assays confirmed the presence of slight but significant cytotoxic and genotoxic events associated with the US-nanoprobe combined treatments. our results can provide novel hints towards US and nanomedicine combined strategies for cell spectral imaging as well as drug delivery-based therapies

The Plio-Quaternary uplift of the Apennine Chain: new data from the analysis of topography and river valleys in Central Italy

This study aimed at the reconstruction of magnitude and timing of uplift of a wide sector of the Central Apennines (Italy) by means of morphometric and morphostructural analyses. In the internal portion of the chain (where stratigraphic and geomorphological markers of past sea-level positions are lacking) the study was based on analysis of erosional landforms and river valleys. A large-scale topographic analysis was performed, processing 90-m and 230-m DEMs. The spatial distribution of several morphometric parameters, together with characteristic wavelengths of relief, allowed the distinction of three main regions affected by different cumulative surface uplift and tectonic/erosional fragmentation: a Peri-Tyrrhenian Belt; an Axial Belt; a Peri-Adriatic Belt. Particular attention was devoted to fluvial landforms, with analysis of longitudinal profiles and geometric pattern of the main stream-trunks and their relations with major structures. Major differences occur between the Tyrrhenian and Adriatic valley systems, the former being generally longitudinal and showing overall concave-upward longitudinal profiles, whereas the latter are generally transverse and possess less regular longitudinal profiles. Topographic features and river valleys architecture seem related to different styles and amounts of uplift in the three Belts. Within the study area, a narrower coast to coast transect (Gaeta-Vasto Transect, GVT) was investigated in detail, devoting particular attention to its axial sector, lying around the Apennines main divide (main divide area: MDA), and a possible scheme of the Quaternary surface uplift inside this transect was proposed. In the MDA, the main stages of landscape evolution and river network organization were reconstructed by analysis of paleosurfaces coupled with analysis of relic and present-day hydrographic network. This allowed recognition of a major phase of surface uplift (exceeding 1500 m in the Meta-Mainarde massif) occurred in response to thrusting during the Pliocene, whereas for the Quaternary uplift a minimum value around 400 m was estimated. Our study suggests that, during the Quaternary and in the GVT, the Peri-Tyrrhenian Belt suffered a subdued uplift operating over small wavelengths (10-15 km), while Axial and Peri- Adriatic Belts were subject to a stronger and long-wavelength (90 km) surface uplift, with maximum values (about 700 m) shifted NE of the Axial Belt and tapering to zero towards the Adriatic coast. The reconstructed pattern of uplift is coherent with the topographic properties of the three Belts and with the observed drainage features

Role of poly [adp-ribose] polymerase 1 in activating the kirsten ras (Kras) gene in response to oxidative stress

In pancreatic Panc-1 cancer cells, an increase of oxidative stress enhances the level of 7,8-dihydro-8-oxoguanine (8OG) more in the KRAS promoter region containing G4 motifs than in non-G4 motif G-rich genomic regions. We found that H2O2 stimulates the recruitment to the KRAS promoter of poly [ADP-ribose] polymerase 1 (PARP-1), which efficiently binds to local G4 structures. Upon binding to G4 DNA, PARP-1 undergoes auto PARylation and thus becomes negatively charged. In our view this should favor the recruitment to the KRAS promoter of MAZ and hnRNP A1, as these two nuclear factors, because of their isoelectric points >7, are cationic in nature under physiological conditions. This is indeed supported by pulldown assays which showed that PARP-1, MAZ, and hnRNP A1 form a multiprotein complex with an oligonucleotide mimicking the KRAS G4 structure. Our data suggest that an increase of oxidative stress in Panc-1 cells activates a ROS-G4-PARP-1 axis that stimulates the transcription of KRAS. This mechanism is confirmed by the finding that when PARP-1 is silenced by siRNA or auto PARylation is inhibited by Veliparib, the expression of KRAS is downregulated. When Panc-1 cells are treated with H2O2 instead, a strong up-regulation of KRAS transcription is observed

Ultrasound imaging, a stethoscope for body composition assessment

Bone and muscle are two deeply interconnected organs and a strong relationship between them exists in their development and maintenance. The peak of both bone and muscle mass is achieved in early adulthood, followed by a progressive decline after the age of 40. The increase in life expectancy in developed countries resulted in an increase of degenerative diseases affecting the musculoskeletal system. Osteoporosis and sarcopenia represent a major cause of morbidity and mortality in the elderly population and are associated with a significant increase in healthcare costs. Several imaging techniques are currently available for the non-invasive investigation of bone and muscle mass and quality. Conventional radiology, dual energy X-ray absorptiometry (DXA), computed tomography (CT), magnetic resonance imaging (MRI) and ultrasound often play a complementary role in the study of osteoporosis and sarcopenia, depicting different aspects of the same pathology. This paper presents the different imaging modalities currently used for the investigation of bone and muscle mass and quality in osteoporosis and sarcopenia with special emphasis on the clinical applications and limitations of each technique and with the intent to provide interesting insights into recent advances in the field of conventional imaging, novel high-resolution techniques and fracture risk

Live single cell analysis using synchrotron FTIR microspectroscopy: development of a simple dynamic flow system for prolonged sample viability

Synchrotron radiation Fourier transform infrared microspectroscopy (SR-microFTIR) of live biological cells has the potential to provide far greater biochemical and morphological detail than equivalent studies using dehydrated, chemically-fixed single cells. Attempts to measure live cells using microFTIR are complicated by the aqueous environment required and corresponding strong infrared absorbance by water. There is also the additional problem of the limited lifetime of the cells outside of their preferred culture environment. In this work, we outline simple, cost-effective modifications to a commercially available liquid sample holder to perform single live cell analysis under an IR microscope and demonstrate cell viability up to at least 24 hours. A study using this system in which live cells have been measured at increasing temperature has shown spectral changes in protein bands attributed to α-β transition, consistent with other published work, and proves the ability to simultaneously induce and measure biochemical changes. An additional study of deuterated palmitic acid (D31-PA) uptake at different timepoints has made use of over 200 individual IR spectra collected over ∼4 hours, taking advantage of the ability to maintain viable cell samples for longer periods of time in the measurement environment, and therefore acquire greatly increased numbers of spectra without compromising on spectral quality. Further developments of this system are planned to widen the range of possible experiments, and incorporate more complex studies, including drug-cell interaction