Effective boundary conditions for dense granular flows

We derive an effective boundary condition for granular flow taking into account the effect of the heterogeneity of the force network on sliding friction dynamics. This yields an intermediate boundary condition which lies in the limit between no-slip and Coulomb friction; two simple functions relating wall stress, velocity, and velocity variance are found from numerical simulations. Moreover, we show that this effective boundary condition corresponds to Navier slip condition when GDR MiDi's model is assumed to be valid, and that the slip length depends on the length scale that characterises the system, \emph{viz} the particle diameter.Comment: 4 pages, 5 figure

Shear bands in granular flow through a mixing length model

We discuss the advantages and results of using a mixing-length, compressible model to account for shear banding behaviour in granular flow. We formulate a general approach based on two function of the solid fraction to be determined. Studying the vertical chute flow, we show that shear band thickness is always independent from flowrate in the quasistatic limit, for Coulomb wall boundary conditions. The effect of bin width is addressed using the functions developed by Pouliquen and coworkers, predicting a linear dependence of shear band thickness by channel width, while literature reports contrasting data. We also discuss the influence of wall roughness on shear bands. Through a Coulomb wall friction criterion we show that our model correctly predicts the effect of increasing wall roughness on the thickness of shear bands. Then a simple mixing-length approach to steady granular flows can be useful and representative of a number of original features of granular flow.Comment: submitted to EP

Characterization of virulence factors in the newly described <i>Salmonella enterica</i> serotype Keurmassar emerging in Senegal (sub-Saharan Africa)

From 2000 to 2001, nine strains of Salmonella enterica belonging to the new serotype Keurmassar have been isolated from human and poultry samples at the Senegalese National Salmonella and Shigella Reference Laboratory at the Pasteur Institute, in Dakar. All strains carried virulence factors including Salmonella Pathogenicity Islands (SPI)-1, -2, -3 and -5 encoded genes. Strains did not harbour virulence plasmid. Ribotyping analysis revealed a single clone identical to Salmonella Decatur isolated in Zimbabwe. These data suggest that strains are closely related, and may have been spread clonally. In this new serotype, insertion sequence IS200 is not present

Maternal and fetal fatty acid composition in ovine muscle tissues

In species characterized by a cotyledonary placenta, as sheep, the relative contribution of maternally derived and placenta synthesized fatty acids is not fully understood. For this reason, the FA composition of mother muscle and the deposition of FA in the fetal muscle were studied by gas-chromatography. Five pregnant Sarda ewes were slaughtered at approximately 145 days of pregnancy. Semitendinosus, semimembranosus and femoral biceps muscles were immediately removed from ewes and fetuses. Data were analyzed by a paired t-test, to detect differences in FA composition between fetus and mother tissues. Results showed that FA profile of fat muscle differed markedly between fetus and mother. The intramuscular fat content were 6.38% and 11.79% on DM basis in fetus and mother muscle, respectively. Linoleic (LA; 18:2n-6), and linolenic (ALA; 18:3n-3) acid were found at smaller concentrations in fetus (0.77 and 0.01 mg/100 mg total FA for LA and ALA, respectively) than in maternal muscle (5.55 and 1.04 for LA and ALA, respectively). On the contrary, a higher proportion of their long-chain polyunsaturated metabolites, like arachidonic (AA; 20:4n-6) and docoexadienoic (DHA; 22:6n-3) acid in fetus compared to mother tissue (AA, 2.32 vs 1.30; DHA, 0.83 vs 0.09 mg/100 mg total FA) indicates a preferential fetal accumulation of those FA which are important for fetuses growth and central nervous system development. The c9,t11 Conjugated linoleic acid (CLA) isomer concentration in mother tissue was 0.72 mg/100 mg FA. It was found also in fetus muscle (0.11 mg/100 mg total FA) suggesting a transplacental fatty acid passage to fetal tissues or a desaturation activity on vaccenic acid (VA; 18:1 t11) in the placental or in the fetal tissue. Surprisingly, the c9,t11-CLA was not the most abundant CLA isomer found in fetus fat. Others CLA isomers, separated but not identified, were found in concentration of 0.17, 0.15 and 0.53 mg/100 mg of FA. Concentrations of these CLA isomers were lower in mother tissue (0.12, 0.05 and 0.10 mg/100 mg of FA). The concentration of VA was 0.46 and 0.90 in fetus and mother muscle, respectively. Correlation analysis between FA profile of mothers and fetuses evidenced a close relationship only for odd-numbered long-chain fatty (r = -0.72 for C15:0 and r = -0.88 for C17:0). The results suggested a different FA metabolism in the muscle tissue of mother and fetus in dairy sheep

JNK plays a key role in tau hyperphosphorylation in Alzheimer's disease models

Alzheimer's disease (AD) is a major clinical concern, and the search for new molecules to combat disease progression remains important. One of the major hallmarks in AD pathogenesis is the hyperphosphorylation of tau and subsequent formation of neurofibrillary tangles. Several kinases are involved in this process. Amongst them, c-Jun N-terminal kinases (JNKs) are activated in AD brains and are also associated with the development of amyloid plaques. This study was designed to investigate the contribution of JNK in tau hyperphosphorylation and whether it may represent a potential therapeutic target for the fight against AD. The specific inhibition of JNK by the cell permeable peptide D-JNKI-1 led to a reduction of p-tau at S202/T205 and S422, two established target sites of JNK, in rat neuronal cultures and in human fibroblasts cultures. Similarly, D-JNKI-1 reduced p-tau at S202/T205 in an in vivo model of AD (TgCRND8 mice). Our findings support the fundamental role of JNK in the regulation of tau hyperphosphorylation and subsequently in AD pathogenesis

Lower hybrid resonances stimulated by the four CLUSTER relaxation sounders deep inside the plasmasphere: observations and inferred plasma characteristics

International audienceThe frequency range of the WHISPER relaxation sounder instrument on board CLUSTER, 4–80 kHz, has been chosen so as to encompass the electron gyro-frequency, F ce , and the electron plasma frequency, F p , in most regions to be explored. Measurement of those frequencies, which are triggered as resonances by the sounder, provides a direct estimation of in situ fundamental plasma characteristics: electron density and magnetic field intensity. In the late mission phase, CLUSTER penetrated regions deep inside the plas-masphere where F ce and F p are much higher than the upper frequency of the sounder's range. However, they are of the right order of magnitude as to place the lower hybrid frequency , F lh , in the 4–15 kHz band. This characteristic frequency , placed at a resonance of the medium, is triggered by the sounder's transmitter and shows up as an isolated peak in the received spectrum, not present in spectra of naturally occuring VLF waves. This paper illustrates, from analysis of case events, how measured F lh values give access to a plasma diagnostic novel of its kind. CLUSTER, travelling along its orbit, encounters favourable conditions where F ce is increasing and F p decreasing, such that F ce /F p increases from values below unity to values above unity. Measured F lh values thus give access, in turn, to the effective mass, M eff , indicative of plasma ion composition, and to the core plasma-sphere electron density value, a parameter difficult to measure. The analysed case events indicate that the estimated quantities (M eff in the 1.0–1.4 range, N e in the 5 × 10 2 – 10 4 cm −3 range) are varying with external factors (altitude, L value, geomagnetic activity) in a plausible way. Although covering only a restricted region (mid-latitude, low altitude inner plasmasphere), these measurements are available, since Correspondence to: S. Kougblénou ([email protected]) late 2009, for all CLUSTER perigee passes not affected by eclipses (on average, roughly a third of a total of ∼200 passes per year) and offer multipoint observations previously unavailable in this region

In search of phylogenetic congruence between molecular and morphological data in bryozoans with extreme adult skeletal heteromorphy

peerreview_statement: The publishing and review policy for this title is described in its Aims & Scope. aims_and_scope_url: http://www.tandfonline.com/action/journalInformation?show=aimsScope&journalCode=tsab20© Crown Copyright 2015. This document is the author's final accepted/submitted version of the journal article. You are advised to consult the publisher's version if you wish to cite from it

An Exploratory Assessment of Applying Risk Management Practices to Engineered Nanomaterials

The widespread industrial application of nanotechnology has increased the number of workers exposed to engineered nanomaterials (ENMs), but it is not clear to what extent prevention guidance is practiced. Our aim was to explore the extent that companies manufacturing and/or using ENMs apply risk assessment and management measures. Thirty-four companies were surveyed with an international 35-item questionnaire investigating company and workforce features, types of ENM handled, and risk evaluation and preventive measures adopted. Among participating companies, 62% had a maximum of 10 employees. Metal-based nanomaterials were most frequently identified (73%). Environmental monitoring was performed by 41% of the companies, while engineering exposure controls were approximately reported by 50%. Information and training programs were indicated by 85% of the sample, only 9% performed specific health surveillance for ENM workers. Personal protective equipment primarily included gloves (100%) and eye/face protection (94%). This small-scale assessment can contribute to the limited amount of published literature on the topic. Future investigations should include a greater number of companies to better represent ENM workplaces and a direct access to industrial settings to collect information on site. Finally, deeper attention should be paid to define standardized frameworks for ENM risk assessment that may guide nano-specific preventive actions

Alfvén waves in the near-PSBL lobe: Cluster observations

Electromagnetic low-frequency waves in the magnetotail lobe close to the PSBL (Plasma Sheet Boundary Layer) are studied using the Cluster spacecraft. The lobe waves show Alfvénic properties and transport their wave energy (Poynting flux) on average toward the Earth along magnetic field lines. Most of the wave events are rich with oxygen (O+) ion plasma. The rich O+ plasma can serve to enhance the magnetic field fluctuations, resulting in a greater likelihood of observation, but it does not appear to be necessary for the generation of the waves. Taking into account the fact that all events are associated with auroral electrojet enhancements, the source of the lobe waves might be a substorm-associated instability, i.e. some instability near the reconnection site, or an ion beam-related instability in the PSBL

Trim17, novel E3 ubiquitin-ligase, initiates neuronal apoptosis

Accumulating data indicate that the ubiquitin-proteasome system controls apoptosis by regulating the level and the function of key regulatory proteins. In this study, we identified Trim17, a member of the TRIM/RBCC protein family, as one of the critical E3 ubiquitin ligases involved in the control of neuronal apoptosis upstream of mitochondria. We show that expression of Trim17 is increased both at the mRNA and protein level in several in vitro models of transcription-dependent neuronal apoptosis. Expression of Trim17 is controlled by the PI3K/Akt/GSK3 pathway in cerebellar granule neurons (CGN). Moreover, the Trim17 protein is expressed in vivo, in apoptotic neurons that naturally die during post-natal cerebellar development. Overexpression of active Trim17 in primary CGN was sufficient to induce the intrinsic pathway of apoptosis in survival conditions. This pro-apoptotic effect was abolished in Bax(-/-) neurons and depended on the E3 activity of Trim17 conferred by its RING domain. Furthermore, knock-down of endogenous Trim17 and overexpression of dominant-negative mutants of Trim17 blocked trophic factor withdrawal-induced apoptosis both in CGN and in sympathetic neurons. Collectively, our data are the first to assign a cellular function to Trim17 by showing that its E3 activity is both necessary and sufficient for the initiation of neuronal apoptosis. Cell Death and Differentiation (2010) 17, 1928-1941; doi: 10.1038/cdd.2010.73; published online 18 June 201