Seed germination and seedling growth of canola treated with abiotic factors that can affect growth and development.

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The lncRNA HOTAIR transcription is controlled by HNF4α-induced chromatin topology modulation

The expression of the long noncoding RNA HOTAIR (HOX Transcript Antisense Intergenic RNA) is largely deregulated in epithelial cancers and positively correlates with poor prognosis and progression of hepatocellular carcinoma and gastrointestinal cancers. Furthermore, functional studies revealed a pivotal role for HOTAIR in the epithelial-to-mesenchymal transition, as this RNA is causal for the repressive activity of the master factor SNAIL on epithelial genes. Despite the proven oncogenic role of HOTAIR, its transcriptional regulation is still poorly understood. Here hepatocyte nuclear factor 4-α (HNF4α), as inducer of epithelial differentiation, was demonstrated to directly repress HOTAIR transcription in the mesenchymal-to epithelial transition. Mechanistically, HNF4α was found to cause the release of a chromatin loop on HOTAIR regulatory elements thus exerting an enhancer-blocking activity

Terapia fotodinâmica aplicada ao tratamento do carcinoma epidermóide em uma gata

O artigo não apresenta resumo

Is the genetic variability of elite rice in southern Brazil really disappearing?

There is a worldwide concern about a possible narrowing of the genetic base of most crops, as e.g. that of rice (Oryza sativa L.), as a result of the modern breeding practices. Thus, the purpose of this study was to investigate this phenomenon in the germplasm of elite paddy rice in southern Brazil, including frequently used accessions in crosses. The panel consisted of 91 accessions. Data of morphological traits, SNP markers and mineral content of husked and polished grain were analyzed by hierarchical clustering and principal component analysis. The SNP markers and hierarchical clustering proved most appropriate to assess the genetic variability. A narrowing of the genetic base of rice was confirmed, although a certain level of genetic variability was still found in the germplasm of elite paddy rice in south Brazilian rice, particularly for grain mineral content

Monitoramento de Leishmaniose tegumentar em cães de Porto Alegre

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Exploring Serum Levels of Brain Derived Neurotrophic Factor and Nerve Growth Factor Across Glaucoma Stages

To investigate the serum levels of Brain Derived Neurotrophic Factor (BDNF) and Nerve Growth Factor (NGF) in patients affected by primary open angle glaucoma with a wide spectrum of disease severity compared to healthy controls and to explore their relationship with morphological and functional glaucoma parameters.45 patients affected by glaucoma at different stages and 15 age-matched healthy control subjects underwent visual field testing, peripapillary retinal nerve fibre layer thickness measurement using Spectral Domain Optical Coherence Tomography and blood collection for both neurotrophins detection by Enzyme-Linked Immunosorbent Assay. Statistical analysis and association between biostrumental and biochemical data were investigated.Serum levels of BDNF in glaucoma patients were significantly lower than those measured in healthy controls (261.2±75.0 pg/ml vs 313.6±79.6 pg/ml, p = 0.03). Subgroups analysis showed that serum levels of BDNF were significantly lower in early (253.8±40.7 pg/ml, p = 0.019) and moderate glaucoma (231.3±54.3 pg/ml, p = 0.04) but not in advanced glaucoma (296.2±103.1 pg/ml, p = 0.06) compared to healthy controls. Serum levels of NGF in glaucoma patients were significantly lower than those measured in the healthy controls (4.1±1 pg/mL vs 5.5±1.2 pg/mL, p = 0.01). Subgroups analysis showed that serum levels of NGF were significantly lower in early (3.5±0.9 pg/mL, p = 0.0008) and moderate glaucoma (3.8±0.7 pg/ml, p<0.0001) but not in advanced glaucoma (5.0±0.7 pg/ml, p = 0.32) compared to healthy controls. BDNF serum levels were not related to age, visual field mean deviation or retinal nerve fibre layer thickness either in glaucoma or in controls while NGF levels were significantly related to visual field mean deviation in the glaucoma group (r2 = 0.26, p = 0.004).BDNF and NGF serum levels are reduced in the early and moderate glaucoma stages, suggesting the possibility that both factors could be further investigated as potential circulating biomarkers for the early detection of glaucoma

Mapping QTLs for root architecture and yield in maize using a field-grown introgression library.

XXIII EUCARPIA MAIZE AND SORGHUM CONFERENCE. - GENOMICS AND PHENOMICS FOR MODEL-BASED MAIZE AND SORGHUM BREEDING, Montpellier, 10-12 giugno 2015 Mapping QTLs for root architecture and yield in maize using a field-grown introgression library Silvia Giuliani1; Carlos Busanello2; Pierluigi Meriggi3; Ana K. Martinez A.1; Roberto Tuberosa1; Antonio Lopez4, Silvio Salvi1 1 Department of Agricultural Sciences, University of Bologna, Viale Fanin 44, 40127 Bologna, Italy ([email protected]) 2 Faculdade de Agronomia Eliseu Maciel, Universidade Federal de Pelotas, Pelotas-RS, CEP 96001-970, Brasil 3 Horta srl Via Egidio Gorra 55, 29122 Piacenza, Italy 4 National Research Council (CNR), Water Research Institute (IRSA), Via Salaria km 29,300 C.P. 10 00015 Monterotondo Stazione, Rome, Italy In crops, the genetic control of root architecture variation in the field and its relationship with yield are incompletely known. A 75 introgression-line (IL) collection from the maize cross B73 x Gaspé Flint was previously shown to segregate for several agronomically important traits (Salvi et al. 2011, BMC Plant Biology). The aim of our study was to investigate the presence of genetic variation for root traits at the adult stage in the field, and its effect on grain yield. For this purpose, the IL collection was grown in a replicated field trial at two water regimes (well-watered, WW, and water-stressed, WS). Forty-four traits covering phenology, plant architecture, yield, and root architecture were analyzed. Root architecture was investigated by means of shovelomics (Trachsel et al. 2011, Plant and Soil) coupled with software-assisted digital image analysis (REST, Colombi et al. 2015, Plant and Soil), for a total of c. 1,400 analyzed root images. The stress imposed in the WS experiment resulted in significantly lower yield (-42%) and longer anthesis-silking interval (+9%) when compared with WW. In WS, statistically significant lower root dry weight and higher number of brace roots were also observed. Relatively high correlation values (up to r = 0.66) were observed between visually or manually scored root traits and corresponding traits based on software-based analysis. QTL analysis showed a complex genetic control for most root traits, and only limited overlap between root and yield or yield components QTLs. Together with root and drought-related QTL information based on hydroponics and pot experiments for the same population, these results should contribute to unveil the role of root architecture variation on maize yield. (The financial support of EU FP7 Water4Crops, grant agreement#311933 is gratefully acknowledged). Colombi T, Kirchgessner N, Le Marié CA, York LM, Lynch JP, Hund A (2015) Next generation shovelomics: set up a tent and REST. Plant and Soil 388:1-20. Salvi S, Corneti S, Bellotti M, Carraro N, Sanguineti MC, Castelletti S, Tuberosa R (2011). Genetic dissection of maize phenology using an intraspecific introgression library. BMC Plant Biology 11:4. Trachsel S, Kaeppler SM, Brown KM, Lynch JP (2011) Shovelomics: high throughput phenotyping of maize (Zea mays L.) root architecture in the field. Plant and Soil 341:75-87