A Functional Genomics Approach to Establish the Complement of Carbohydrate Transporters in Streptococcus pneumoniae

The aerotolerant anaerobe Streptococcus pneumoniae is part of the normal nasopharyngeal microbiota of humans and one of the most important invasive pathogens. A genomic survey allowed establishing the occurrence of twenty-one phosphotransferase systems, seven carbohydrate uptake ABC transporters, one sodium∶solute symporter and a permease, underlining an exceptionally high capacity for uptake of carbohydrate substrates. Despite high genomic variability, combined phenotypic and genomic analysis of twenty sequenced strains did assign the substrate specificity only to two uptake systems. Systematic analysis of mutants for most carbohydrate transporters enabled us to assign a phenotype and substrate specificity to twenty-three transport systems. For five putative transporters for galactose, pentoses, ribonucleosides and sulphated glycans activity was inferred, but not experimentally confirmed and only one transport system remains with an unknown substrate and lack of any functional annotation. Using a metabolic approach, 80% of the thirty-two fermentable carbon substrates were assigned to the corresponding transporter. The complexity and robustness of sugar uptake is underlined by the finding that many transporters have multiple substrates, and many sugars are transported by more than one system. The present work permits to draw a functional map of the complete arsenal of carbohydrate utilisation proteins of pneumococci, allows re-annotation of genomic data and might serve as a reference for related species. These data provide tools for specific investigation of the roles of the different carbon substrates on pneumococcal physiology in the host during carriage and invasive infection

Management von traumatologischen orbitalen Fremdkörpern

Im Rahmen von Traumata kann es zum Eindringen von Fremdkörpern in die Orbita kommen, die teilweise auch die Schädelbasis erreichen. Diese seltenen, aber für den Patienten häufig mit gravierenden Konsequenzen einhergehenden Fälle, werden anhand von Beispielen aus unserer Klinik der letzten 5 Jahre vorgestellt. Besonderes Augenmerk wird bei der Therapie auf die Zugangswege, sowie die postoperative Nachsorge gelegt. Hierbei kommt insbesondere der Anwendung eines Navigationssystems eine wichtige Rolle zu