Візуальні репрезентації трансформації маскулінності (Visual representation of transformation of masculinity)

Актуальність цього дослідження можна аргументувати такими тезами: а) розвиток чоловічих досліджень пов’язаний із становленням культури постмодернізму, в якій трансформація маскулінності розглядається не як соціокультурна девіація, а як один із варіантів “нормального” розвитку соціокультурної сфери; б) все більше з’являється візуальних репрезентацій андрогінних образів, і це пов’язано з тим, що суспільство стало нав’язувати сучасному чоловікові якості, які традиційно вважалися жіночими. (The main ideas of this study are: a) the development of male research associated with the formation of postmodern culture in which the transformation of masculinity is not seen as sociocultural deviation, but as one of the following “normal” development of socio-cultural sphere. b) It appears more visual representations androgyny’s images, and this is due to the fact that society has imposed as modern men, which are traditionally considered feminine

Some aspects of the moss population development on the Svalbard glaciers

Glaciers are rather unusual habitat for mosses, but sometimes they can be suitable for some species due to presence of sufficient moisture and cryoconite substrate in the ablation zone. To date, moss populations were found only on a few glaciers in Alaska, Iceland and Svalbard. An origin and development of moss cushions on ice (so called "glacier mice") are still unclear. In this study, some aspects of the moss population development were explored on ice of the Svalbard glaciers – Bertilbreen (Billefjorden) and Austre Grnfjordbreen (Grnfjorden) in 2012 and 2013. On Bertilbreen, populations of Hygrohypnella polaris (Lindb.) Ignatov & Ignatova and Schistidium abrupticostatum (Bryhn) Ignatova & H.H. Blom were found for the first time. Due to putative morphological features, identification of S. abrupticostatum was confirmed by com-paring ITS1-2 nrDNA sequence data to BLAST searches (megablast). The results indicated a genetic heterogeneity of the population. Although visually moss-free, examination of cryoconite sediments revealed development of new individuals of S. abrupticostatum from filamentous structures consisting of caulonema and rhizoids. The developmental stages of young plants were revealed. Therefore, besides fragmen-tation of existing cushions, cryoconite sediments provided a source of new moss cush-ions in glacier populations. Additionally a few plants of Pohlia cf. wahlenbergii (F. Weber & D. Mohr) Andrews and a gametophyte fragment of Philonotis sp. were found in aggregation of cryoconite. Presence of Paludella squarrosa (Hedw.) Brid. reported for Bertilbreen has not been confirmed. On Austre Grnfjordbreen Bryum cryophilum Mrtensson, Sanionia uncinata (Hedw.) Loeske were found invading into some Hygrohypnella polaris cushions. Each moss polster on ice represents a separate mini-ecosystem that includes successive colonization events

Variability of Winter Wheat Quality Features in Northern Trans-Urals

The studies were carried out in laboratory conditions on the basis of the Agrotechnological Institute of Northern Trans-Ural State Agricultural University. The studies include 5 varieties of winter wheat grown in 2009-2011 in three agroclimatic zones of Tyumen Region: sub-regional (Nizhnetavdinsky state crop testing site), northern forest steppe (Yalutorovsky state crop testing site), southern forest steppe (Berduzhsky state crop testing site). Bashkirskaya variety – 10 (45 g) demonstrated the highest potential in the formation of a 1000 grain weight. The same variety has the highest range of variability (11.7 g) and the variability coefficient (10.9%) indicates average variability. Other varieties showed minor variability of characteristic (V = 8.7-9.9%). Winter wheat varieties varied in terms of average hardness, at the same time the level of values of all varieties (66-77%) corresponded to the standards of high classes according to GOST. Novosibirskayaaya 32 (61-93%) and Bashkirskaya 10 (60-86%) varieties were the most stable in forming the required value of characteristic. The average gluten content in a grain of winter wheat varieties was corresponded to the standards of the third class according to GOST. Novosibirskaya 32 variety (V = 8.5%) had minor variability of the characteristic, while other varieties demonstrated the average variability. A significant proportion of the effect of the “variety” factor on the variability of such features as a 1000 grain weight (56.5%), grain-unit (50.5%) and hardness (45.1%) was established. The amount of gluten depended more on the growing environment (35.5%) and interaction of factors (39.3%). The quality of gluten was much influenced by the growing environment (52.2%)

Targeting tumor multicellular aggregation through IGPR-1 inhibits colon cancer growth and improves chemotherapy

Adhesion to extracellular matrix (ECM) is crucially important for survival of normal epithelial cells as detachment from ECM triggers specific apoptosis known as anoikis. As tumor cells lose the requirement for anchorage to ECM, they rely on cell-cell adhesion 'multicellular aggregation' for survival. Multicellular aggregation of tumor cells also significantly determines the sensitivity of tumor cells to the cytotoxic effects of chemotherapeutics. In this report, we demonstrate that expression of immunoglobulin containing and proline-rich receptor-1 (IGPR-1) is upregulated in human primary colon cancer. Our study demonstrates that IGPR-1 promotes tumor multicellular aggregation, and interfering with its adhesive function inhibits multicellular aggregation and, increases cell death. IGPR-1 supports colon carcinoma tumor xenograft growth in mouse, and inhibiting its activity by shRNA or blocking antibody inhibits tumor growth. More importantly, IGPR-1 regulates sensitivity of tumor cells to the chemotherapeutic agent, doxorubicin/adriamycin by a mechanism that involves doxorubicin-induced AKT activation and phosphorylation of IGPR-1 at Ser220. Our findings offer novel insight into IGPR-1's role in colorectal tumor growth, tumor chemosensitivity, and as a possible novel anti-cancer target.Grant support from: R01 CA175382/CA/NCI NIH HHS/United States, R21 CA191970/CA/NCI NIH HHS/United States, and R21 CA193958/CA/NCI NIH HHS/United State

The individual strategies of Internet users’ media activity

The article is dedicated to the analyses of actual strategies of Internet users’ media activity in the ecosystem of today’s social media (social networks, blogs, hostings, messengers, mobile applications, etc.). The base of the author’s approach that specifies the novelty of the research lies on the analyses of the users’ media activity as a complex of media consumption and production which make a dialectical unity, as well as on the problematization of subject oriented research optics which draws attention to the individual as a direct media subject. The interaction of the individual and media ecosystem is analyzed with the help of a system of incoming and outcoming activity markers. The incoming activity is understood by the authors as a total of institutional and environmental factors of media activity practices realization, the outcoming one is understood as the characteristic of individual’s personal activity in realizing media practices. It is shown that the configuration of the dominant types of incoming and outcoming activity forms the individual media strategy. It is theoretically based and empirically proved by the author’s sociological research with the use of factor analyses (online poll of social network users, N = 1000) that there practically exist four types of individual media strategies: inclusively open, exclusively open, inclusively conservative and exclusively conservative ones. It is found out that the majority of the Internet users (38,3%) use the exclusively open strategy, estimate modern media conditions as too liberal and thus needing control and restrictions. In the conclusion there are shown the perspective directions of the following scientific enquiry on the topic of the research

TECHNOSPHERE: PHILOSOPHICAL CONCEPTS, DEVELOPMENT TRENDS AND PRACTICAL CHALLENGES

The global technological environment, which mediates the interaction of mankind with the biosphere, carries technogenic and environmental hazards and serious anthropological and social risks. Today there is a tendency in which every improvement of the technogenic system ultimately increases its negative impact on nature on the principle of negative feedback. In such a situation, an objective assessment of the technosphere’s characteristics is necessary for timely harmonization in the relationship “Human – Natural Environment”. The purpose of the article is to conceptualize ideas about the technogenic path of human development and to introduce the concept of “ecological technosphere” as a new stage of coevolutionary relations between nature and technogenic civilization. The research is carried out within the framework of the concept of post-classical science, a structural and functional approach, postphenomenology, actor-network theory. In the article, based on the concepts of “technique”, “technology”, “technocenosis”, “technological environment”, an ontological analysis of the concept of “technosphere” is proposed, its essential characteristics and risky scenarios of development are highlighted. The article was carried out within the framework of the project “Socio-ecological monitoring of the technical environment of the region: a socio-cultural approach” (RFBR Grant 20-311-90060 Graduate students

B Cell Depletion Therapy as a Cutting-Edge Treatment of Demyelinating Diseases of the Central Nervous System

Demyelinating diseases of the central nervous system and multiple sclerosis in particular are a pressing issue for medical community and society as a whole. Deve- lopment and implementation of highly effective specific therapy significantly slow the disease progression and help maintain patients' quality of life and social participation. We analyzed pathogenic mechanisms of multiple sclerosis and other B cell-mediated diseases and reviewed therapeutic options for main disease stages

Epithelial cell–derived secreted and transmembrane 1a signals to activated neutrophils during pneumococcal pneumonia

Airway epithelial cell responses are critical to the outcome of lung infection. In this study, we aimed to identify unique contributions of epithelial cells during lung infection. To differentiate genes induced selectively in epithelial cells during pneumonia, we compared genome-wide expression profiles from three sorted cell populations: epithelial cells from uninfected mouse lungs, epithelial cells from mouse lungs with pneumococcal pneumonia, and nonepithelial cells from those same infected lungs. Of 1,166 transcripts that were more abundant in epithelial cells from infected lungs compared with nonepithelial cells from the same lungs or from epithelial cells of uninfected lungs, 32 genes were identified as highly expressed secreted products. Especially strong signals included two related secreted and transmembrane (Sectm) 1 genes, Sectm1a and Sectm1b. Refinement of sorting strategies suggested that both Sectm1 products were induced predominantly in conducting airway epithelial cells. Sectm1 was induced during the early stages of pneumococcal pneumonia, and mutation of NF-kB RelA in epithelial cells did not diminish its expression. Instead, type I IFN signaling was necessary and sufficient for Sectm1 induction in lung epithelial cells, mediated by signal transducer and activator of transcription 1. For target cells, Sectm1a bound to myeloid cells preferentially, in particular Ly6GbrightCD11bbright neutrophils in the infected lung. In contrast, Sectm1a did not bind to neutrophils from uninfected lungs. Sectm1a increased expression of the neutrophil-attracting chemokine CXCL2 by neutrophils from the infected lung. We propose that Sectm1a is an epithelial product that sustains a positive feedback loop amplifying neutrophilic inflammation during pneumococcal pneumonia

14-color flow cytometry to determine the contribution of mitochondrial mass to differences in glycolytic capacity in human immune cell subsets

Mitochondrial metabolism controls immune cell function, but comprehensive tools to assess human primary immune cell metabolic capacity remain rudimentary. We previously demonstrated that CD19+ B cells rely more heavily on anaerobic glycolysis (i.e. are more glycolytic) than CD4+ T cells. Furthermore, both PBMCs and CD4+ T cells from subjects with type 2 diabetes (T2D) are more glycolytic than their counterparts from BMI-matched non-T2D controls. The contribution of mitochondrial mass, an indicator of non-glycolytic metabolism, to the various metabolic phenotypes is untested. To assess the contribution of immune cell subset identity and mitochondrial mass to the enhanced glycolytic capacity of resting B cells and PBMCs from T2D subjects, we designed a 13-color panel based on standard immune cell subset markers and chemokine receptors, and included MitoTracker Green FM (MTG), which quantitatively indicates mitochondrial mass. We used this novel panel to phenotype 63 total samples from BMI-matched subjects in three groups: non-T2D, pre-T2D, and fulminant T2D, as defined by American Diabetes Association guidelines. The panel was built in several iterations to accommodate spillover of MTG fluorescence into neighboring channels and includes, besides MTG and live-dead discriminator, the following surface markers: CD4, CD8, CD19, CD45RA, CD25, CD127, CD14, CCR4, CCR5, CCR6, CXCR3, and CD161. The PBMC samples were run on a 4-laser BD FACSARIA II SORP with pre-established panel-specific PMT voltages tracked using 6-peak Ultrarainbow beads. To normalize MTG fluorescence intensity and thus minimize batch effects, each of 5 total batches included a reference donor PBMC sample that was frozen in multiple aliquots from one blood draw. Using this approach, we quantified the percentages of immune cell populations (CD19+ B cells, CD8+ naïve and memory/effector T cells, and CD4+ cells including Tregs and populations enriched in Th1, Th2 and Th17) along with the relative mitochondrial mass in each subset. We found that CD19+ B cells in PBMCs from both ND and T2D subjects had significantly less mitochondrial mass than CD4+ cells, supporting the demonstration that B cells are more glycolytic than CD4+ T cells. Of all the CD4+ T cell subsets, Th17 cells consistently had the lowest mitochondrial mass, consistent with the interpretation that Th17s are more dependent on glycolysis than previously appreciated. Our results validate the utility of our 13-color panel to simultaneously quantify relative mitochondrial mass in numerous immune cell subsets and thereby provide a new tool to explore metabolism in human primary cells

Non-language factors and language evolution

This paper is devoted to the description of the functioning and development of the language system seen in correlation with the influence of non-language factor