47 research outputs found
Laser Focusing System for High Brightness Polarized Electron Source for SPLEEM
For construction of a high brightness polarized electron source for SPLEEM (Spin Polarized Low Energy Electronic Microscope), a new transmission type photocathode has been developed at Nagoya Univ. In our scheme, a laser light is injected from backside of the photocathode and an electron beam is emitted forward from the surface. A high numerical aperture lens with a short focal length can focus the laser lights into a small spot of photocathode. A laser spot with a diameter of 2.2μm could be achieved by our laser system, and it contributed to realize the high beam brightness of 10^5A·cm^{-2}·sr^{-1} or more for a total current of 5μA.journal articl
Effects of atropine and propranolol on glucose- and arginine-induced insulin release in rats
departmental bulletin pape
DataFile3.csv
The fitted results of Figs. 4(e)-(h). The table shows the nonlinear curve fitting results of MP-hFCS measurements in a single cell expressing either GFP or GFP-GRalpha wt
DataFile4.csv
Mean diffusion constants and diffusion times for GFP and GFP-Gralpha wt in HeLa cells. This table gives the averages and standard deviations of the nonlinear curve fitting results of MP-hFCS measurement (n=3) of single cells expressing either GFP or GFP-GRwt before and after Dex treatment
DataFile1.csv
Fitted results of rhodamine 6G solution. This table gives the averages and standard deviations of the nonlinear curve fitting results measured in different days (n=13)
DataFile2.csv
Comparison of the results measured by MP-hFCS and ConfoCor 2. This table gives the averages and standard deviations of the nonlinear curve fitting results of three different dyes
Systematic Error in Fluorescence Correlation Measurements Identified by a Simple Saturation Model of Fluorescence
The distortion of the fluorescence correlation function of
a dye solution becomes larger with the increase in the
excitation power, and eventually the parameters, such as
the number of molecules and the diffusion time, obtained
by the fluorescence correlation function systematically
change. The most fundamental reason for this change is
thought to be the distortion of the Gaussian excitation−detection field due to the saturation of the photocycle of
the chromophore. The deviation from linearity of the
fluorescence intensity causes the distortion of the fluorescence correlation function. Consequently, a smaller
excitation power reduces the distortion and ensures an
accurate measurement of the absolute value of these
parameters. At the same time, the measurements at a
fixed excitation power can be used to quantitatively
determine the relative value of concentration and of the
diffusion time. The deviation in the linearity of the
fluorescence intensity and the deviation of the parameters
show a high degree of correlation, and a 10% deviation
of the intensity results in a prediction of a ∼10% deviation
in the number of molecules and a ∼5% in the diffusion
time
