47 research outputs found

    Laser Focusing System for High Brightness Polarized Electron Source for SPLEEM

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    For construction of a high brightness polarized electron source for SPLEEM (Spin Polarized Low Energy Electronic Microscope), a new transmission type photocathode has been developed at Nagoya Univ. In our scheme, a laser light is injected from backside of the photocathode and an electron beam is emitted forward from the surface. A high numerical aperture lens with a short focal length can focus the laser lights into a small spot of photocathode. A laser spot with a diameter of 2.2μm could be achieved by our laser system, and it contributed to realize the high beam brightness of 10^5A·cm^{-2}·sr^{-1} or more for a total current of 5μA.journal articl

    吹奏楽演奏者に対するイメージの研究1<人文・社会科学>

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    Observation of B→K*ℓ+ℓ-

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    The fitted results of Figs. 4(e)-(h). The table shows the nonlinear curve fitting results of MP-hFCS measurements in a single cell expressing either GFP or GFP-GRalpha wt

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    Mean diffusion constants and diffusion times for GFP and GFP-Gralpha wt in HeLa cells. This table gives the averages and standard deviations of the nonlinear curve fitting results of MP-hFCS measurement (n=3) of single cells expressing either GFP or GFP-GRwt before and after Dex treatment

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    Fitted results of rhodamine 6G solution. This table gives the averages and standard deviations of the nonlinear curve fitting results measured in different days (n=13)

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    Comparison of the results measured by MP-hFCS and ConfoCor 2. This table gives the averages and standard deviations of the nonlinear curve fitting results of three different dyes

    Systematic Error in Fluorescence Correlation Measurements Identified by a Simple Saturation Model of Fluorescence

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    The distortion of the fluorescence correlation function of a dye solution becomes larger with the increase in the excitation power, and eventually the parameters, such as the number of molecules and the diffusion time, obtained by the fluorescence correlation function systematically change. The most fundamental reason for this change is thought to be the distortion of the Gaussian excitation−detection field due to the saturation of the photocycle of the chromophore. The deviation from linearity of the fluorescence intensity causes the distortion of the fluorescence correlation function. Consequently, a smaller excitation power reduces the distortion and ensures an accurate measurement of the absolute value of these parameters. At the same time, the measurements at a fixed excitation power can be used to quantitatively determine the relative value of concentration and of the diffusion time. The deviation in the linearity of the fluorescence intensity and the deviation of the parameters show a high degree of correlation, and a 10% deviation of the intensity results in a prediction of a ∼10% deviation in the number of molecules and a ∼5% in the diffusion time
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