Development of the Microwave SQUID Multiplexer for an X-ray Microcalorimeter Array of Transition Edge Sensor

埼玉大学博士(理学)主指導教員 : 寺田 幸功textapplication/pdfdoctoral thesi

Development of the Microwave SQUID Multiplexer for an X-ray Microcalorimeter Array of Transition Edge Sensor

学位記号番号 : 博理工乙第274号博士の専攻分野の名称 : 博士(理学) 学位授与年月日 : 令和4年9月22日textapplication/pdfthesi

ジキ ネンダンセイ リュウタイ ノ リュウドウ トクセイ ト オウヨウ テンカイ ニカンスル ケンキュウ

本研究では懸濁系の磁気粘弾性流体の流動特性と圧力特性について四要素モデルを用いて考察を行う．実験では，磁気粘弾性流体がオリフィスを流れる際の圧力損失について，スタートアップの圧力変化と磁場印加時の圧力の過渡的な挙動を測定した．また実験結果の検証として数値解析も行った．その結果，磁気粘弾性流体の流れは，ケルビン力，磁気スピンの作用，物性を決定づける4要素モデルによって数値的に表現されることを明らかにした．The flow and pressure characteristics of magneto-viscoelastic fluids in suspension systems are discussed in this study using a four-element model. In the experiment, the pressure drop of the magneto-viscoelastic fluid flowing through the orifice was measured in terms of pressure change at startup and transient pressure behavior when a magnetic field is applied. Numerical analysis was also performed to verify the experimental results. The results show that the flow of a magneto-viscoelastic fluid can be numerically described by a four-element model that determines the Kelvin force, the action of magnetic spin, and physical properties.application/pdfdoctoral thesi

Observation of B→K*ℓ+ℓ-

journal articl

テロリストに対する自衛権の適用可能性

departmental bulletin pape

カレツキの経済政策論－完全雇用の政治経済学－

2009-06This paper considers the modern relevance of Kalecki's ideas on economic policies. While he believed in the advantages of socialism as an economic system, he also searched for the ways to relieve evils of capitalist economies such as unemployment, poverty and inequality. Basing on the view that the insufficiency of effective demand was the main cause of unemployment, he proposed the methods of government spending on public investment or subsidies to mass consumption, and redistribution of income from higher-to lower-income class in order to achieve full employment. However, Kalecki considered that in capitalist economies there would be substantial political and social obstacles to maintain full employment. He asserted that 'industrial leaders' would oppose full employment achieved by government spending because sustained full employment would enhance the power of the working class and shake the political stability of capitalism. Further, he was skeptical of the possibility of development of capitalist economies. Because economic policies are made as a result of conflict between the interests of various groups in the capitalist camp, intervention will never be consistent. Kalecki shows a deep insight into the working of social conflict in capitalist economies.departmental bulletin pape

NF-κB exists in a complex with dynein/dynactin <i>in vivo.</i>

<p>NF-κB p65 co-immunoprecipitates with dynactin and cytoplasmic dynein. 10 mg soluble rat brain extract were cross-linked with DSP to stabilize transient protein-protein interactions and subsequent immunoprecipitated with an anti-p65 antibody. Immunoprecipitates were analyzed by western blotting with an anti- NF-κB p65 or NF-κB p50 antibody or with antibodies against dynein intermediate chain (IC 74) or the motor-associated proteins dynamitin and p150^Glued. Control IP was done with a non-immune monoclonal antibody.</p

Microtubule-perturbing drugs inhibit neuronal transport of NF-κB.

<p>(A) Effect of microtubule-perturbing drugs on the organization of neuronal microtubule network. Microtubule network was visualized by α−tubulin immunostaining. Treatment of hippocampal neurons with 200 nM colchicine or 100 nM vincristine resulted in efficient depolymerisation, as shown by the diffuse tubulin staining and the loss of the well-organized microtubule patterns seen in untreated cells.(B) Hippocampal neurons treated with 300 µM glutamate for 5 min, either alone or after pre-treatment with 200 nM colchicine or 100 nM vincristine for 30 min were fixed (90 min after glutamate exposure) and visualised by SYTOX nuclear staining (green) and anti-NF-κB p65 immunofluorescence (red) to monitor neuronal transport of NF-κB (C) Quantification of nuclear/dendritic ratio of α-p65 fluorescence in neurons with functional or disrupted microtubules. Note that microtubule-perturbing drugs impaired dendritic to nuclear redistribution of NF-κB p65 after glutamate stimulation. (D) Reporter gene assay showed reduced NF-κB-dependent transcription activity in neurons with not functional microtubules. (E) In order to confirm the data obtained by microscopy, the subcellular localization of p65 was examined by cell fractionation and Western blotting. 250 µg of soluble protein from cytoplasmic extracts were immunoblotted for p65 protein level. In agreement, with the above experiments, pre-treatment with vincristine or colchicines before exposure to glutamate resulted in reduced relocation of p65 from cytoplasm to the nucleus.</p

Schematic presentation for the NF-κB activation by synaptic activity and its dynein-mediated retrograde transport to the nucleus along microtubules.

<p>Upon stimulation of primary neurons with glutamate, different signalling pathways (represented here by CaMKII) originating from intracellular Ca²⁺ elevation induces phosphorylation of IκB, which subsequently leads to its degradation within the proteasome. Thereby, the nuclear localisation signals (NLS) of NF-κB subunits are unmasked, allowing its binding to importin α/β heterodimer. This complex is then transported retrogradely towards the nucleus via an association with motor protein dynein/dynactin, where it activates NF-κB target genes.</p

Inhibition of dynein function by overexpression of dynamitin impairs nuclear accumulation of NF-κB and reduces NF-κB-dependent transcription activity.

<p>(A) Cultured hippocampal neurons transfected with dynamitin or mock vector were left untreated or incubated with 300 µM glutamate for 5 min. After 90 min neurons were fixed and analyzed for subcellular NF-κB p65 destribution. Nuclei were stained with SYTOX (green). Anti-p65 immunoreactivity is depicted in red. (B) Quantification of nuclear/dendritic ratio of α-p65 fluorescence in dynamitin-transfected and control-transfected neurons with and without glutamate stimulation. Note that transfection with dynamitin impaired redistribution of NF-κB p65 from distal sites to the nucleus following glutamate stimulation. Fluorescence measurements were made from 20 to30 neurons in each experimental condition. (C) Reporter gene assay showed reduced NF-κB-dependent transcription activity in neurons overexpressing dynamitin.</p