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departmental bulletin pape

マウス神経系に発現するプレキシンファミリ-分子群の単離同定

名古屋大学Nagoya University博士(理学)プレキシン(plexin)はアフリカツメガエルの神経系において発見された分子量220kdの膜蛋白である｡アフリカツメガエルにおいてプレキシンはカルシウムイオン依存性のホモフィリックな細胞接着分子であり､神経細胞間の相互作用に関与していると考えられている｡プレキシンの生体内での機能を解明するために､ジーンターゲッティングやトランスジェニック法により個体レベルで遺伝子操作の可能なマウス神経系で発現するプレキシン相同分子の検索を試みた｡マウス胎児脳のcDNAライブラリーのスクリーニングを行った結果､プレキシン類似分子を3種類単離同定することができた｡マウスプレキシン1はアフリカツメガエルプレキシンと全体で84%のアミノ酸配列のアイデンティティーがあり､アフリカツメガエルプレキシンと同様に細胞外領域にc-Met蛋白質と相同性の高い3つのシステインリッチドメインを持っていた｡また､マウスプレキシン2及びマウスプレキシン3も細胞外領域の3つのシステインリッチドメインは保存されていた｡マウスプレキシン1とマウスプレキシン2の間では62%､マウスプレキシン1とマウスプレキシン3の間では66%､マウスプレキシン2とマウスプレキシン3の間では59%のアミノ酸配列の相同性があった｡このように､プレキシンは脊椎動物の種を越えて広く存在する分子であり､マウスにおいては､プレキシンは類似した3種類の分子からなる分子ファミリー(プレキシンファミリー)を構成していることが明らかとなった｡また､ホモロジ､一検索の結果､これらプレキシンファミリー分子はc-Met受容体型チロシンリン酸化酵素ファミリー以外には既知の蛋白質との類似性は見られず､新しいタイプの膜蛋白質ファミリーであることが明らかとなった｡ノーザンブロッティングの結果､マウスプレキシン1､マウスプレキシン2､マウスプレキシン3の転写産物は発生期の脳で強く検出され､成体脳では低かった｡また､神経系以外の組織のノーザンブロツティングを行ったところ､3種のプレキシンはそれぞれ違った組織で発現していることが明らかとなった｡しかしながら､神経系に比べ非神経組織での発現レベルは低かった｡これらの結果から､マウス神経系においてプレキシンは多様な分子ファミリーを構成しており､特異的な神経回路網の形成や維持に重要な役割を果たしていると考えられる｡名古屋大学博士学位論文 学位の種類:博士(理学) (課程) 学位授与年月日:平成9年3月25日doctoral thesi

Factors Associated with Increased Caregiver Burden of Informal Caregivers during the COVID-19 Pandemic in Japan

application/pdfJournal of Nutrition, Health and Aging. 2022, 26 (2), P.157-160journal articl

インターネット調査によるセンチメント観測 : 消費動向・景気見通しからみえる傾向〔論文〕

多くのインターネット調査は調査会社のアクセスパネルに登録している人を対象に実施しているため，世論調査や社会調査に求められる一般市民の代表性がない．しかしながら、アクセスパネルが偏っていることを受け入れながらインターネット調査による定点観測を続けることで，世の中の変化をつぶさに知ることができる． 本研究ではインターネット調査による定点観測データ（Macromill Weekly Index）を用いて，過去3年の週単位での消費金額や景況感の動きを外部データと比較して，高い相関性があることを確認した．また，内閣支持や支持政党によって消費や景況感に違いが生じていることを明らかにした． Many online surveys have been conducted on the people who are registered with the access panels of research companies, and they are not representative of the general public, which is a requirement for both public opinion and social surveys. However, by continuing a fixed-point observation of online surveys, while accepting that the access panel is biased, it is possible to determine world changes immediately. In this study, by using fixed-point observation data from an online survey (Macromill Weekly Index), as compared to the movements of consumption amounts and business confidence on a weekly basis for the past three years as well as external data, it was confirmed that there is a high correlation. It was also revealed that the differences in consumption amounts and economic outlook are caused by the Cabinet’s support and political support parties.１．はじめに ２．マクロミル定点観測調査について ３．消費者動向と景況感 ４．景況感と政治意識 ５．おわりにtextapplication/pdfdepartmental bulletin pape

Observation of B+→pp̅π+, B0→pp̅K0, and B+→pp̅K*+

journal articl

Corrosion Diagnosis Investigation of Archaeological Iron Objects in Exhibition Case of Tottori Mukibanda Yayoi Settlement Site

departmental bulletin pape

THE DECOMPOSITION THEOREMS FOR VECTOR MEASURES

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Presentation_1_Antagonizing FcαR1 (CD89) as treatment in IgA-mediated chronic inflammation and autoimmunity.pdf

IntroductionImmunoglobulin A (IgA) is mostly considered as a non-inflammatory regulator at mucosal areas. However, previous work of our group showed that IgA can also be involved in disease pathology, because it provides a potent stimulus to activate neutrophils after crosslinking of surface CD89 (FcaRI), resulting in chronic inflammation and tissue damage. IgA (auto)antibodies and neutrophils are key players in various diseases, including blistering skin diseases and rheumatoid arthritis. Therefore, we generated an array of anti-CD89 monoclonal antibodies (mAbs) for therapeutic targeting of CD89. The biological activity of newly developed anti-human CD89 mAbs and their potential therapeutic capacity were investigated.MethodsHuman neutrophils were isolated from heparinized healthy donor blood. The ability of anti-CD89 mAbs to bind human neutrophils was investigated by flow cytometry. Furthermore, the capacity of these anti-CD89 mAbs to inhibit IgA-mediated phagocytosis, neutrophil extracellular trap (NET) release and migration was studied. To this end, neutrophils were pre-incubated with/without anti-CD89 mAbs after which they were stimulated with IgA-coated beads. The amount of phagocytosed beads, NET release and migrated neutrophils were subsequently analysed. In parallel, chemoattractant leukotriene B4 and lactoferrin (as a measure for degranulation) release were determined. Finally, the therapeutic potential of our prototypic anti-CD89 mAb clone 10E7 was in vivo tested in anti-mouse collagen XVII human IgA-treated transgenic CD89 mice, a preclinical model for autoimmune linear IgA bullous disease (LABD).ResultsOur results show that all generated anti-CD89 mAbs bound surface CD89 on neutrophils. Although these anti-CD89 mAbs bind to different epitopes on EC1 of CD89, they all have the capacity to inhibit IgA-mediated phagocytosis, neutrophil extracellular trap (NET) release and neutrophil migration. Moreover, IgA mediated leukotriene B4 and lactoferrin release are decreased in supernatant from anti-CD89 mAbs-treated neutrophils. Finally, anti-CD89 mAb clone 10E7, that was selected based on its selective binding profile on tissue micro arrays, reduced anti-mouse collagen XVII hIgA-induced neutrophil influx in an in vivo linear IgA bullous disease (LABD) mice model.ConclusionThis study clearly indicates that our newly developed anti-CD89 mAbs inhibited IgA-induced neutrophil activation and reduced anti-autoantigen IgA-induced neutrophil influx in vivo, supporting further clinical development for the treatment of LABD.</p

Additional file 4 of Enhanced IgA coating of bacteria in women with Lactobacillus crispatus-dominated vaginal microbiota

Additional file 3: Figure S3. Immunoglobulin levels in vaginal fluid over time. Unbound immunoglobulins were measured menstrual bleeding; time point 1 (T = 1), 7-11 days after onset of menstrual bleeding; time point 2 (T = 2) and 17-25 days after onset of menstrual bleeding; time point 3 (T = 3). The level of (A) unbound IgA1, (B) unbound IgA2 and (C) unbound SIgA over time. All data visualized as 10log of the unbound immunoglobulin concentration corrected for total protein. Red line represents the mean. * p < 0.05 ** p < 0.01

Additional file 5 of Enhanced IgA coating of bacteria in women with Lactobacillus crispatus-dominated vaginal microbiota

Additional file 4: Figure S4. The ratio between bound and unbound immunoglobulins. Bound and unbound immunoglobulins were measured during menstrual bleeding; time point 1 (T = 1), 7-11 days after onset of menstrual bleeding; time point 2 (T = 2) and 17-25 days after onset of menstrual bleeding; time point 3 (T = 3). Bound immunoglobulins (coating index) were divided by unbound immunoglobulins (concentration) to calculate the ratio. Red line represents the mean