バイオエレクトロニクスと耐熱性酵素の利用<定例セミナー>

textapplication/pdfdepartmental bulletin pape

Observation of B+→pΛ̅γ

journal articl

現代中国の環境公害訴訟に関する一考察―福建省寧徳市の化学工場汚染事件に焦点を当てて―

2008-07-24加藤久和教授退職記念論文集departmental bulletin pape

数理モデルの構築と運用のための会話型計算システムの開発 : 環境拡散モデルを例として

application/pdfComplicated programing has been necessary to get a result of mathematical simulation models. But, NIFE（Numerator of Itemized Formula Expressions）is a new tool to make and use them on MS-DOS. NIFE extracts results of values, tables, and graphs from ordinary itemized formula expressions and vector-matrix operations, of which a simulation model is made. NIFE has performance of calculating, listing, searching, modifying, and saving them. And also, NIFE has external libraries like numerical analysis of differential equation and method of least squares. So, anybody may briefly operate a model with NIFE.departmental bulletin pape

分子三脚の秩序配列に基づく長寿命三重項カルベン単分子膜の開発

application/pdf三重項カルベンは、中性2配位の炭素原子に2個の不対電子をもつ有機分子種であり、安定化が困難な活性種の一つである。本研究では、三重項カルベンの長寿命化を目的として、その前駆体を我々が開発した剛直な「分子三脚」を用いてAu基板表面に自己組織化単分子膜として固定した。膜上への光照射により発生したカルベンは基板上への束縛をうけて2分子的分解反応を起こさないため、溶液中で発生させた場合と比べて安定化することが確認できた。Triplet carbenes are highly reactive organic species containing a divalent neutral carbon atom. It is expected that immobilization on a solid surface is an effective strategy to make triplet carbenes longer-lived. We formed self-assembled monolayers of carbene precursors on Au substrate using a ""molecular tripod ""as an anchor. The carbene generated by irradiation of the monolayer are found to be more stabilized than in solution, due to the forbidden bimolecular decomposition.平成21~23年度科学研究費補助金(基盤研究(C))研究成果報告書21550132research repor

Innate immune responses through Toll-like receptor 3 require human-antigen-R-mediated Atp6v0d2 mRNA stabilization

Toll-like receptor 3 (TLR3) recognizes double-stranded RNA derived from virus and its synthetic analogue, polyinosinic?polycytidylic acid [poly(I:C)]. Upon poly(I:C) binding, TLR3 activates transcription factors to express inflammatory cytokines and type I interferon. TLR3 is located in the endosomes and its recognition of poly(I:C) and activation of downstream signaling is regulated by endosomal acidification. However, the mechanism of post-transcriptional regulation in TLR3-mediated innate responses remains unclear. Here, we focused on Human antigen R (HuR, also known as ELAVL1) that recognizes and binds to the 3′ untranslated regions (3′UTRs) of target mRNAs, thereby protecting them from mRNA degradation, and found that HuR-deficient murine macrophage cells showed significantly reduced Ifnb1 mRNA expression after poly(I:C) stimulation. HuR-deficient cells also showed a marked reduction in the expression of Atp6v0d2 mRNA, which encodes a subunit of vacuolar-type H+ ATPase (V-ATPase), and therefore reduced endosomal acidification. HuR associated with the 3′UTR of Atp6v0d2 mRNA and the stability of Atp6v0d2 mRNA was maintained by its association with HuR. Taken together, our results suggest that HuR stabilizes Atp6v0d2 mRNA, which is required for the TLR3-mediated innate immune responses.journal articl