<Original Article> A Degradative Process Suggestive of Autophagic Death in Post-Senescent Cells of Yeast Observed by Light Microscopy

We previously reported that when virgin temperature-sensitive mutant edc28 cells of the yeast Saccharomyces cerevisiae were incubated at restrictive temperatures, they lost proliferative activity in 24 hr and thereafter cellular components became progressively degraded resulting in extensive fragmentation in 7 days in parallel with morphological changes suggestive of autophagocytosis. The process was named autophagic death. Here, to test whether yeast cells in the post-senescent life span undergo autophagic death, the edc28 cells were incubated at the permissive temperature and the cells that ceased the cell cycle during bud formation were observed by light microscopy for 7 days. Most cells that stopped the cell cycle produced an abnormally large bud. They accumulated vesicles within 24 hr after the arrest of the cell cycle and became shrunken and thin cytoplasm at day 7. A control strain without cdc mutation showed essentially the same morphological changes as the cdc28 senescent cells, suggesting that the biological process suggestive of autophagic death under light microscopy occurred in the post-senescence life span of the cells irrespective of cdc mutations.journal articl

An in-phantom dosimetry system using pin silicon photodiode radiation sensors for measuring organ doses in x-ray CT and other diagnostic radiology

A dosimetry system using commercially available pin silicon photodiodes as the sensor is evaluated for in-phantom dose measurements in x-ray CT and other diagnostic radiology. System sensitivity measured as a function of the effective energy of x rays was between 0.37 and 0.49 V/mGy at an effective energy range between 23.5 and 72 keV. The minimum detectable organ dose with 25% uncertainty was estimated to be 0.02 mGy. The excellent output linearity was found over a dose range from 0.03 to more than 10 mGy with flat dose rate response of system sensitivity up to 35 mGy s^-1, though the sensitivity indicated some energy dependence across the diagnostic energy range with a maximum of about 10%/10 keV. Since angular dependence of the sensitivity of the photodiode sensor was found to be small enough it would induce negligible dose error. Dose profile measurement along the axis of a thoracic phantom undergoing CT chest examination indicated the reliability of dose values over a range of two orders of magnitude from less than 0.2 to 12 mGy. The present dosimetry system having advantages of high sensitivity with immediate readout of dose values, low cost, and easy construction would widely be used as an alternative to TLD dosimeters for organ and skin dose measurements in CT and other diagnostic radiology.journal articl

『中学教科単語帳』（日本語?タイ語）刊行記念 ■■■座談会■■■

一般雑誌記事 / Articlearticl

Investigation about the continuity of the sign in a transit center (1)

In this research, at JR Hiroshima Station and JR Okayama Station, the route was set up and investigation about the continuity of a sign was conducted. At JR Hiroshima Station, the sign of the route I exists continuously. However, by the route II, a Shinkansen exit and an underground passage have few signs. At JR Okayama Station, the station square of the route I has few signs, and a sign exists in the underground passage of the route II continuously. At JR Hirosima Station, there is much duplication of sign information and a user may get confused.departmental bulletin pape

Improved measurement of CP-violation parameters sin⁡2ϕ1 and |λ|, B meson lifetimes, and B0-B̅0 mixing parameter Δmd

journal articl

第7回アメリカ研修旅行報告―ＲＩＴ・ＮＴＩＤ・ＧＡＬＬＡＵＤＥＴ大学のキャンパス配置・教育の概要について―

1998年3月22日から3月31日まで、アメリカのロチェスター工科大学（RIT）、国立ろう工科大学（NTID）、ギャローデット大学（Ga11audet uiversity）の視察研修旅行に参加した。本稿では、この視察の概要、各大学のキャンパス配置、教育の概要について報告する。departmental bulletin pape

Additional file 1: of Differences in gene expression in whitefly associated with CYSDV-infected and virus-free melon, and comparison with expression in whiteflies fed on ToCV- and TYLCV-infected tomato

a. The number of Illumina raw and processed reads produced per RNA-Seq library from virus free (“VF”) whiteflies, Bemisia tabaci MEAM1, and whiteflies fed on cucurbit yellow stunting disorder virus (CYSDV) infected melon plants (“CYSDV”) for 24 h, 72 h, and 7 d. b. Correlation matrix analysis for multiple biological replicates obtained from RNA-Seq libraries prepared from virus free (VF) whiteflies fed on uninfected melon plants and whiteflies fed on cucurbit yellow stunting disorder virus (CYSDV) infected melon plants for periods of for 24 h, 72 h, and 7 d. c. Differentially expressed genes between virus free (VF) whiteflies fed on uninfected melon and whiteflies fed on cucurbit yellow stunting disorder virus (CYSDV) infected melon plants for 24 h, 72 h, and 7 d. (XLSX 33 kb

Additional file 4: of Differences in gene expression in whitefly associated with CYSDV-infected and virus-free melon, and comparison with expression in whiteflies fed on ToCV- and TYLCV-infected tomato

A list of 69 differentially regulated orphan genes found in CYSDV whiteflies compared to VF whiteflies following feeding for 72 h and 7 days. (XLSX 21 kb

Additional file 3: of Differences in gene expression in whitefly associated with CYSDV-infected and virus-free melon, and comparison with expression in whiteflies fed on ToCV- and TYLCV-infected tomato

A list of 59 common differentially expressed genes between ToCV whiteflies and CYSDV whiteflies compared to virus free whiteflies at all three different feeding time points. (XLSX 33144 kb

Additional file 2: of Differences in gene expression in whitefly associated with CYSDV-infected and virus-free melon, and comparison with expression in whiteflies fed on ToCV- and TYLCV-infected tomato

a. KEGG annotation, KO, and score values of the 28 genes out of a total of 82 up-regulated in CYSDV whiteflies versus virus-free whiteflies at 72 h. b. Pathway reconstruction results from the 28 genes out of a total of 82 up-regulated in CYSDV whiteflies versus virus-free whiteflies at 72 h. c. KEGG annotation, KO, and score values of the 40 genes out of a total of 139 down-regulated in CYSDV whiteflies versus virus-free whiteflies at 72 h. d. Pathway reconstruction results from the 40 genes out of a total of 139 down-regulated in CYSDV whiteflies versus virus-free whiteflies at 72 h. e. KEGG annotation, KO, and score values of the 12 genes out of a total of 49 up-regulated in CYSDV whiteflies versus virus-free whiteflies at 7 d. f. Pathway reconstruction results from the 12 genes out of a total of 49 up-regulated in CYSDV whiteflies versus virus-free whiteflies at 7 d. (XLSX 49 kb