Acidification of stratum corneum prevents atopic march in murine models

의과대학/박사Maintenance of acidic pH in the stratum corneum(SC) is an important factor for normal skin barrier function. Long standing or repeated skin barrier damage followed by atopic dermatitis(AD) is the initial step of the atopic march that eventually progresses to respiratory allergies. We determined whether various AD murine models, flaky tail, Nc/Nga and multiple oxazolone(Ox) challenged mice, can develop airway inflammation by topical application and nasal inhalation of a house dust mite, Dermatofagoides pteronyssinus(Dp)(defined as a novel ‘atopic march animal model’), and whether maintenance of an acidic SC environment by continuous application of acidic cream can interrupt this atopic march. During the course of Dp treatment to the atopic march murine model, an acidic cream with pH 2.8 and neutral cream(pH7.4) adjusted by citric acid and sodium hydroxide mixed with vehicle were applied twice daily. Repeated applications and inhalations of Dp to AD murine model induced AD skin lesions followed by respiratory allergies. These results indicate that novel atopic march models can be developed by repeated application of house dust mites on flaky tail, Nc/Nga and Ox-induced AD mice, and that the acidification of SC could be a novel intervention method to block the progress of the atopic march.ope