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    ์‚ฌ๋‹จ๊ธ‰ ์ดํ•˜ ์˜๋ฌด์‹œ์„ค ๊ฐ„ํ˜ธ์žฅ๊ต๋ฅผ ์œ„ํ•œ ์ง๋ฌด๊ต์œก ์š”๊ตฌ๋„

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    ๊ฐ„ํ˜ธ๋Œ€ํ•™/์„์‚ฌ๋ณธ ์—ฐ๊ตฌ๋Š” ์‚ฌ๋‹จ๊ธ‰ ์ดํ•˜ ์˜๋ฌด์‹œ์„ค ๊ฐ„ํ˜ธ์žฅ๊ต๋“ค์˜ ์ง๋ฌด๊ต์œก ์š”๊ตฌ๋„์™€ ์šฐ์„ ์ˆœ์œ„๋ฅผ ํŒŒ์•…ํ•˜์—ฌ ํ–ฅํ›„ ์ง๋ฌด๊ต์œก ํ”„๋กœ๊ทธ๋žจ ๋งˆ๋ จ์˜ ๊ธฐ์ดˆ์ž๋ฃŒ๋ฅผ ์ œ๊ณตํ•˜๊ณ ์ž ์‹œ๋„๋˜์—ˆ๋‹ค. ์—ฐ๊ตฌ๋Š” 2๋‹จ๊ณ„๋กœ ์ง„ํ–‰๋˜์—ˆ๊ณ , 1๋‹จ๊ณ„๋Š” ์—ฐ๊ตฌ ๋Œ€์ƒ์ž์˜ ์ง๋ฌด๊ต์œก ์š”๊ตฌ๋„๋ฅผ ํŒŒ์•…ํ•˜๊ธฐ ์œ„ํ•œ ๋ฌธํ•ญ ๊ฐœ๋ฐœ๋กœ, ๋ฌธํ—Œ๊ณ ์ฐฐ ๋ฐ ํฌ์ปค์Šค ๊ทธ๋ฃน ์ธํ„ฐ๋ทฐ๋ฅผ ํ†ตํ•ด ๊ตฌ์„ฑ๋˜์—ˆ๋‹ค. 2๋‹จ๊ณ„๋Š” ์—ฐ๊ตฌ ๋Œ€์ƒ์ž์˜ ์ง๋ฌด๊ต์œก ์š”๊ตฌ๋„๋ฅผ ์ธก์ •ํ•˜๊ธฐ ์œ„ํ•œ ์„œ์ˆ ์  ์กฐ์‚ฌ ์—ฐ๊ตฌ๋กœ, ์‚ฌ๋‹จ๊ธ‰ ์ดํ•˜ ์˜๋ฌด์‹œ์„ค์— ๊ทผ๋ฌดํ•˜๊ฑฐ๋‚˜ ์ตœ๊ทผ 3๋…„ ์ด๋‚ด์— ๊ทผ๋ฌดํ•œ ๊ฒฝํ—˜์ด ์žˆ๋Š” ๊ฐ„ํ˜ธ์žฅ๊ต ์ด 83๋ช…์„ ๋Œ€์ƒ์œผ๋กœ ์ง„ํ–‰ํ•˜์˜€๋‹ค. ์ž๋ฃŒ ์ˆ˜์ง‘์€ 2015๋…„ 10์›” 14์ผ๋ถ€ํ„ฐ 11์›” 15์ผ๊นŒ์ง€ 4์ฃผ๊ฐ„ ์‹ค์‹œํ•˜์˜€๋‹ค. ์ˆ˜์ง‘๋œ ์ž๋ฃŒ์˜ ๋ถ„์„์€ SPSS 21.0 ํ”„๋กœ๊ทธ๋žจ์„ ์ด์šฉํ•˜์—ฌ ๊ธฐ์ˆ ํ†ต๊ณ„, t-test, ANOVA, Mann-Whitney U test, Kruskal-Wallis test๋ฅผ ์‹œํ–‰ํ•˜์˜€๊ณ , ์ง๋ฌด๊ต์œก ์š”๊ตฌ๋„์™€ ์šฐ์„ ์ˆœ์œ„๋Š”Borich ๊ต์œก ์š”๊ตฌ๋„ ๊ณต์‹์„ ์ด์šฉํ•˜์—ฌ ๋„์ถœํ•˜์˜€๋‹ค. ๋ณธ ์—ฐ๊ตฌ์˜ ์ฃผ์š” ๊ฒฐ๊ณผ๋Š” ๋‹ค์Œ๊ณผ ๊ฐ™๋‹ค. 1. ์ง๋ฌด๊ต์œก ์š”๊ตฌ๋„ ์ธก์ •์„ ์œ„ํ•œ ๋ฌธํ•ญ์€ ๋ฌธํ—Œ๊ณ ์ฐฐ ๋ฐ ํฌ์ปค์Šค ๊ทธ๋ฃน ์ธํ„ฐ๋ทฐ๋ฅผ ํ†ตํ•ด ๊ฐœ๋ฐœ๋˜์—ˆ๊ณ , ์ตœ์ข…์ ์œผ๋กœ โ€˜๊ฐ„ํ˜ธ์‹ค๋ฌด์žโ€™, โ€˜๊ต์œก์žโ€™, โ€˜๊ด€๋ฆฌ์žโ€™, โ€˜์ง€๋„์žโ€™ ์˜ 4๊ฐœ ์˜์—ญ๊ณผ 52๊ฐœ ๋ฌธํ•ญ์œผ๋กœ ๊ตฌ์„ฑ๋˜์—ˆ๋‹ค. 2. ๋ณธ ์—ฐ๊ตฌ ๋Œ€์ƒ์ž๋Š” ๋Œ€๋ถ€๋ถ„ ์—ฌ์„ฑ์œผ๋กœ, ํ‰๊ท  ์—ฐ๋ น์€ 26.58ยฑ2.32์„ธ์ด๋ฉฐ, ๊ฒฐํ˜ผ ์ƒํƒœ๋Š” ๋ฏธํ˜ผ, ์ตœ์ข…ํ•™๋ ฅ์€ ๋Œ€ํ•™๊ต ์กธ์—…์ž๊ฐ€ ๋งŽ์•˜๋‹ค. ํ˜„์žฌ ๊ณ„๊ธ‰์€ ์ค‘์œ„๊ฐ€ ๋Œ€์œ„๋ณด๋‹ค ๋งŽ์•˜์œผ๋‚˜ ๋น„์Šทํ•œ ๋ถ„ํฌ๋ฅผ ๋‚˜ํƒ€๋ƒˆ๊ณ , ์ด ์ž„์ƒ๊ฒฝ๋ ฅ์€ 1๋…„ ์ด์ƒ 3๋…„ ๋ฏธ๋งŒ์ด ๊ฐ€์žฅ ๋งŽ์•˜์œผ๋ฉฐ, ์‚ฌ๋‹จ๊ธ‰ ์ดํ•˜ ์˜๋ฌด์‹œ์„ค ๊ทผ๋ฌด ๊ฒฝ๋ ฅ์€ 1๋…„ ์ด์ƒ 2๋…„ ๋ฏธ๋งŒ์ด ๋งŽ์•˜๋‹ค. ์‚ฌ๋‹จ๊ธ‰ ์ดํ•˜ ์˜๋ฌด์‹œ์„ค ๊ทผ๋ฌด ์‹œ ์ง๋ฌด๊ต์œก์„ ๋ฐ›์€ ๋Œ€์ƒ์ž๊ฐ€ ๋” ๋งŽ์•˜๊ณ , ๋Œ€๋ถ€๋ถ„์˜ ๋Œ€์ƒ์ž๊ฐ€ ์‚ฌ๋‹จ๊ธ‰ ์ดํ•˜ ์˜๋ฌด์‹œ์„ค ๊ทผ๋ฌด ์‹œ ์ง๋ฌด๊ต์œก์ด ํ•„์š”ํ•˜๋‹ค๊ณ  ์‘๋‹ตํ•˜์˜€๋‹ค. 3. ๋ณธ ์—ฐ๊ตฌ ๋Œ€์ƒ์ž์˜ ์ง๋ฌด ์ค‘์š”๋„๋Š” 520์  ๋งŒ์  ์ค‘ ์ด์  402.91ยฑ50.06์ , ์ตœ๋Œ€ํ‰๊ท ํ‰์  10์ ์— ํ‰๊ท  7.74์ ์œผ๋กœ โ€˜๊ต์œก์žโ€™ ์˜์—ญ์ด ๊ฐ€์žฅ ๋†’์•˜๊ณ , ์ง๋ฌด ์ˆ˜ํ–‰๋„๋Š” ์ด์  379.78ยฑ57.19์ , ํ‰๊ท  7.30์ ์œผ๋กœ โ€˜๊ต์œก์žโ€™ ์˜์—ญ์ด ๊ฐ€์žฅ ๋†’๊ฒŒ ๋‚˜ํƒ€๋‚ฌ๋‹ค. ์ค‘์š”๋„์™€ ์ˆ˜ํ–‰๋„์˜ ์ฐจ์ด๋Š” ์ด์  23.68ยฑ51.01์ , ํ‰๊ท  0.44์ ์œผ๋กœ, ๊ฐ€์žฅ ์ฐจ์ด๊ฐ€ ํฌ๊ฒŒ ๋‚˜ํƒ€๋‚œ ์˜์—ญ์€ โ€˜๊ด€๋ฆฌ์žโ€™, ๋ฌธํ•ญ์€ โ€˜๊ฐ„ํ˜ธ์—…๋ฌด๋ถ„์žฅโ€™ ์œผ๋กœ ๋‚˜ํƒ€๋‚ฌ๋‹ค. 4. ์ง๋ฌด๊ต์œก ์š”๊ตฌ๋„๋ฅผ Borich ๊ต์œก ์š”๊ตฌ๋„ ๊ณต์‹์œผ๋กœ ํŒŒ์•…ํ•œ ๊ฒฐ๊ณผ, ๊ฐ€์žฅ ๊ต์œก ์š”๊ตฌ๋„๊ฐ€ ๋†’๊ฒŒ ๋‚˜ํƒ€๋‚œ ์˜์—ญ์€ โ€˜๊ด€๋ฆฌ์žโ€™, ๋ฌธํ•ญ์€ โ€˜๊ฐ„ํ˜ธ์—…๋ฌด๋ถ„์žฅโ€™ ์œผ๋กœ ๋‚˜ํƒ€๋‚ฌ๋‹ค. 5. ์ผ๋ฐ˜์  ํŠน์„ฑ์— ๋”ฐ๋ฅธ ์˜์—ญ๋ณ„ ์ง๋ฌด๊ต์œก ์š”๊ตฌ๋„๋ฅผ ๋ถ„์„ํ•œ ๊ฒฐ๊ณผ ์ตœ์ข…ํ•™๋ ฅ, ์ด ์ž„์ƒ๊ฒฝ๋ ฅ, ์‚ฌ๋‹จ๊ธ‰ ์ดํ•˜ ์˜๋ฌด์‹œ์„ค ๊ทผ๋ฌด ๊ฒฝ๋ ฅ, ์‚ฌ๋‹จ๊ธ‰ ์ดํ•˜ ์˜๋ฌด์‹œ์„ค ๊ทผ๋ฌด ์‹œ ์ง๋ฌด๊ต์œก ์—ฌ๋ถ€์— ๋”ฐ๋ผ ํ†ต๊ณ„์ ์œผ๋กœ ์œ ์˜ํ•œ ์ฐจ์ด๊ฐ€ ์žˆ์—ˆ๋‹ค. ์ด์ƒ์˜ ๊ฒฐ๊ณผ์—์„œ ์‚ฌ๋‹จ๊ธ‰ ์ดํ•˜ ์˜๋ฌด์‹œ์„ค ๊ฐ„ํ˜ธ์žฅ๊ต๋“ค์€ ๋ชจ๋“  ์˜์—ญ์—์„œ ๋ณธ์ธ์˜ ์ง๋ฌด์ˆ˜ํ–‰ ๋Šฅ๋ ฅ๋ณด๋‹ค ์ง๋ฌด ์ค‘์š”๋„๋ฅผ ๋” ๋†’๊ฒŒ ํ‰๊ฐ€ํ•˜์˜€๊ณ , 4๊ฐœ ์˜์—ญ ์ค‘ โ€˜๊ด€๋ฆฌ์žโ€™์— ๋Œ€ํ•œ ๊ต์œก์ด ์ตœ์šฐ์„ ์ˆœ์œ„๋กœ ํ™•์ธ๋˜์—ˆ๋‹ค. ๊ฐ„ํ˜ธ์žฅ๊ต ๊ฐœ์ธ์  ์ฐจ์›์—์„œ ์Šค์Šค๋กœ ์ž์งˆ ๊ฐœ๋ฐœ์„ ์œ„ํ•œ ๊พธ์ค€ํ•œ ๋…ธ๋ ฅ์ด ํ•„์š”ํ•˜๊ณ , ์กฐ์ง์  ์ฐจ์›์—์„œ ์‹ค๋ฌด์ž์˜ ๊ต์œก ์š”๊ตฌ๋„๊ฐ€ ๋ฐ˜์˜๋œ ์ง€์†์ ์ด๊ณ  ์ฒด๊ณ„์ ์ธ ์ง๋ฌด๊ต์œก ํ”„๋กœ๊ทธ๋žจ ๊ฐœ๋ฐœ์ด ํ•„์š”ํ•˜๋‹ค.ope

    Properties of calcium phosphate and calcium sulfate hemihydrate composite cement

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    ์น˜์˜ํ•™๊ณผ/์„์‚ฌ[ํ•œ๊ธ€] ์น˜์กฐ๊ณจ ๋ถ€์œ„์™€ ๊ฒฐ์†์„ ํšŒ๋ณต์‹œ์ผœ ์ฃผ๊ธฐ ์œ„ํ•ด ์ƒ์ฒด๊ณจ์ด๋‚˜ ์น˜์•„์™€ ํ™”ํ•™์  ์กฐ์„ฑ์ด ๊ฐ™์€ ์ธ์‚ฐ์นผ์Š˜์„ธ๋ผ๋ฏน์ด ๋งŽ์ด ์‚ฌ์šฉ๋˜๊ณ  ์žˆ์œผ๋‚˜ ์žฌ๋ฃŒ๋ฅผ ๊ฒฐ์†๋ถ€์— ํ•œ์ •์‹œํ‚ค๊ฑฐ๋‚˜ ์„ฑํ˜•ํ•˜๊ธฐ์— ์–ด๋ ค์šด ๋“ฑ์˜ ๋‹จ์ ์ด ์žˆ์–ด ๋ถ„๋ง๊ณผ ์•ก์„ ํ˜ผํ•ฉํ•˜์—ฌ ์‚ฌ์šฉํ•˜๋Š” ์ธ์‚ฐ์นผ์Š˜์‹œ๋ฉ˜ํŠธ๊ฐ€ ๊ฐœ๋ฐœ๋˜์—ˆ๋‹ค. ์ด ์žฌ๋ฃŒ๋Š” ํ˜ผํ•ฉํ•  ๋•Œ๋Š” ์—ฐ๊ณ ํ˜•์ด์—ˆ๋‹ค๊ฐ€ ๊ตฌ๊ฐ•๋‚ด ์‹œ์ˆ  ํ›„ ๊ฒฝํ™”๋˜๋ฏ€๋กœ ์ƒ์ฒด์นœํ™”์„ฑ๋ฟ ์•„๋‹ˆ๋ผ ์กฐ์ง์„ฑ๋„ ์šฐ์ˆ˜ํ•˜๋ฏ€๋กœ ๋งŽ์€ ์—ฐ๊ตฌ๊ฐ€ ์ด๋ฃจ์–ด์ง€๊ณ  ์žˆ์œผ๋‚˜ ๊ฒฝํ™”์‹œ๊ฐ„์ด ๊ธธ๊ณ  ๊ฐ•๋„๊ฐ€ ๋‚ฎ์€ ๋‹จ์ ์ด ์žˆ์–ด ์ด๋ฅผ ํ•ด๊ฒฐํ•˜๊ณ ์ž ๋ณธ ์—ฐ๊ตฌ์—์„œ๋Š” ๋ถ„๋ง์— ์„๊ณ ๋ฅผ ํ˜ผํ•ฉํ•œ ๋ฐ˜์ˆ˜์—ผ์„๊ณ  ์ฒจ๊ฐ€ ์ธ์‚ฐ์นผ์Š˜ ๋ณตํ•ฉ ์‹œ๋ฉ˜ํŠธ๋ฅผ ์ œ์กฐํ•˜์—ฌ ์‹คํ—˜ํ•˜์˜€๋‹ค. ์‹œํ—˜๊ตฐ์€ ๋ถ„๋ง์„ ์ธ์‚ฐ์นผ์Š˜์‹œ๋ฉ˜ํŠธ๋ฅผ ๋‹จ๋…์œผ๋กœ ์‚ฌ์šฉํ•œ ๊ตฐ๊ณผ ๋ฌด๊ฒŒ๋น„๋กœ ๊ฐ๊ฐ 25%, 50%, 75% ์„๊ณ ๋ฅผ ํ˜ผํ•ฉํ•œ ๋ฐ˜์ˆ˜์—ผ์„๊ณ  ์ฒจ๊ฐ€ ์ธ์‚ฐ์นผ์Š˜ ๋ณตํ•ฉ ์‹œ๋ฉ˜ํŠธ๊ตฐ์„ ์‚ฌ์šฉํ•˜์˜€๊ณ  ๋Œ€์กฐ๊ตฐ์€ ๋ฐ˜์ˆ˜์—ผ์„๊ณ ๋ฅผ ๋‹จ๋…์œผ๋กœ ์‚ฌ์šฉํ•˜์˜€์œผ๋ฉฐ ์šฉ์•ก์€ ์ฆ๋ฅ˜์ˆ˜์™€ ํšŒ์„ ์ธ์‚ฐ๋‚˜ํŠธ๋ฅจ์•ก์„ ์‚ฌ์šฉํ•˜์—ฌ ๊ฐ ๋ถ„๋ง๊ณผ ์šฉ์•ก์„ ํ˜ผํ•ฉํ•ด์„œ ๊ฒฝํ™”์‹œ๊ฐ„,์˜จ๋„์ƒ์Šน ๋ฐ ์••์ถ•๊ฐ•๋„ ๋“ฑ์˜ ๋ฌผ๋ฆฌ์ , ๊ธฐ๊ณ„์  ์„ฑ์งˆ, ๊ทธ๋ฆฌ๊ณ  ์ฃผ์‚ฌ์ „์žํ˜„๋ฏธ๊ฒฝ๊ณผ ๋ถ„๋ง X-์„  ํšŒ์ ˆ๋กœ ์ƒ์„ฑ๋œ ์ƒ๊ณผ ๋ฏธ์„ธ๊ตฌ์กฐ๋ฅผ ์—ฐ๊ตฌํ•˜์—ฌ ๋‹ค์Œ๊ณผ ๊ฐ™์€ ๊ฒฐ๊ณผ๋ฅผ ์–ป์—ˆ๋‹ค. 1. ์ฆ๋ฅ˜์ˆ˜์™€ ํ˜ผํ•ฉํ•œ ๋ฐ˜์ˆ˜์—ผ์„๊ณ  ์ฒจ๊ฐ€ ์ธ์‚ฐ์นผ์Š˜ ๋ณตํ•ฉ ์‹œ๋ฉ˜ํŠธ์˜ ๊ฒฝ์šฐ ์„๊ณ ์˜ ํ•จ๋Ÿ‰์ด ์ฆ๊ฐ€ํ• ์ˆ˜๋ก ๊ฒฝํ™”์‹œ๊ฐ„์€ ์งง์•„์ง€๊ณ  ์••์ถ•๊ฐ•๋„๋Š” ์ฆ๊ฐ€ํ•˜์˜€๋‹ค(p<0.05). 2. ํฌ์„ ์ธ์‚ฐ๋‚˜ํŠธ๋ฅจ์•ก๊ณผ ํ˜ผํ•ฉํ•œ ๋ฐ˜์ˆ˜์—ผ์„๊ณ  ์ฒจ๊ฐ€ ์ธ์‚ฐ์นผ์Š˜ ๋ณตํ•ฉ ์‹œ๋ฉ˜ํŠธ์˜ ๊ฒฝ์šฐ ์„๊ณ ์˜ ํ•จ๋Ÿ‰์ด ์ฆ๊ฐ€ํ• ์ˆ˜๋ฅต ๊ฒฝํ™”์‹œ๊ฐ„์€ ๊ธธ์–ด์ง€๊ณ  ์••์ถ•๊ฐ•๋„๋Š” ๊ฐ์†Œํ•˜์˜€์œผ๋ฉฐ ์ฆ๋ฅ˜์ˆ˜๋ฅผ ์‚ฌ์šฉํ•œ ๊ฒฝ์šฐ๋ณด๋‹ค ์œ ์˜์„ฑ์žˆ๊ฒŒ ๋ฌผ๋ฆฌ์  ์„ฑ์งˆ์ด ๋‚ฎ์•˜๋‹ค(p<0.05). 3. ๋ฐ˜์ˆ˜์—ผ์„๊ณ  ์ฒจ๊ฐ€ ์ธ์‚ฐ์นผ์Š˜ ๋ณตํ•ฉ ์‹œ๋ฉ˜ํŠธ๊ตฐ์˜ ๋ฐ˜์‘์˜จ๋„๋Š” 1-2โ„ƒ ์ •๋„ ์ƒ์Šนํ•˜์˜€๋‹ค. 4. ์ฃผ์‚ฌ์ „์žํ˜„๋ฏธ๊ฒฝ ๊ด€์ฐฐ ๊ฒฐ๊ณผ ๊ฒฝํ™”๋œ ๋ฐ˜์ˆ˜์—ผ์„๊ณ  ์ฒจ๊ฐ€ ์ธ์‚ฐ์นผ์Š˜ ๋ณตํ•ฉ ์‹œ๋ฉ˜ํŠธ๋Š” ํฐ ์„๊ณ  ๊ฒฐ์ •๊ตฌ์กฐ ์‚ฌ์ด์— ์ˆ˜์‚ฐํ™”์ธํšŒ์„ ๊ฒฐ์ •๋“ค์ด ๋ญ‰์ณ์ ธ ์กด์žฌํ•˜์˜€๋‹ค. 5. X-์„  ํšŒ์ ˆ ๋ถ„์„ ๊ฒฐ๊ณผ ๊ฒฝํ™” ํ›„ ์ธ์‚ฐ์นผ์Š˜์‹œ๋ฉ˜ํŠธ๋Š” ์ˆ˜์‚ฐํ™”์ธํšŒ์„์œผ๋กœ, ๋ฐ˜์ˆ˜์—ผ์„๊ณ ๋Š” ์ด์ˆ˜์—ผ์„๊ณ ๋กœ ๋Œ€๋ถ€๋ถ„ ์ „ํ™˜๋˜์—ˆ๋‹ค. ๋ฐ˜์ˆ˜์—ผ์„๊ณ  ์ฒจ๊ฐ€ ์ธ์‚ฐ์นผ์Š˜ ๋ณตํ•ฉ ์‹œ๋ฉ˜ํŠธ๋ฅผ ์ฆ๋ฅ˜์ˆ˜์™€ ํ˜ผํ•ฉํ•˜์—ฌ ์‚ฌ์šฉํ•˜๋ฉด ์ธ์‚ฐ์นผ์Š˜์‹œ๋ฉ˜ํŠธ๋ฅผ ๋‹จ๋…์œผ๋กœ ์‚ฌ์šฉํ•œ ๊ฒฝ์šฐ๋ณด๋‹ค ๋ฌผ๋ฆฌ์ , ๊ธฐ๊ณ„์  ์„ฑ์งˆ์ด ์šฐ์ˆ˜ํ•˜์˜€๊ณ  ํŠนํžˆ 75% ๋ฐ˜์ˆ˜์—ผ์„๊ณ  ์ฒจ๊ฐ€ ์ธ์‚ฐ์นผ์Š˜ ๋ณตํ•ฉ ์‹œ๋ฉ˜ํŠธ์˜ ๊ฒฝ์šฐ ๊ฐ€์žฅ ์šฐ์ˆ˜ํ•œ ์„ฑ์งˆ์„ ๋‚˜ํƒ€๋ƒˆ์œผ๋ฏ€๋กœ ์น˜์กฐ๊ณจ ์ด์‹์žฌ๋กœ์„œ ์‚ฌ์šฉํ•˜๊ธฐ์— ์ ํ•ฉํ•  ๊ฒƒ์œผ๋กœ ์‚ฌ๋ฃŒ๋˜์—ˆ๋‹ค. [์˜๋ฌธ] The calcium phosphate cement(CP) and calcium sulfate hemihydrate(CS) composite cements, of which the weight % of CS were 25(CP75), 50(CP5O),75(CP25), 100(CS), were prepared and mixed with either distilled water(S solution) or sodium hydrogen phosphate solution(P solution). The setting times, temperature rises, compressive strengths, microstructures of scanning electron microscopy(SEM) view and powder X-ray diffraction(XRD) patterns of the cements were studied. The results were as follows: 1. When the composite cements mixed with S solution, the more the CS content was, the shorter the setting time and the higher the compressive strength were(p<0.05). 2. When the composite cements mixed with P solution, the more the CS content was, the longer the setting time and the lower the compressive strength were, and they showed significantly inferior physical and mechanical properties compared with the groups using S solution(p<0.05). 3. The temperature rise was about 1โˆผ2โ„ƒ in the composite cement groups. 4. SEM photographs showed that the fully set composite cements consisted of large gypsum crystals mixed with small rod-like hydroxyapatite crystals. 5. XRD revealed that CP was mostly converted to hydroxyapatite and CS was to calcium sulfate dihydrate after reaction, respectively. The conclusion was that the composite cements mixed with S solution had superior physical and mechanical properties to the CP alone and especially the CP75-S group showed the best physical and mechanical properties in this study, so it was the CP75-S group that had the potential to be used as periodontal bone graft materials.restrictio

    Proliferation and differentiation of L929 and MC3T3-E1 cells of calcium phosphate glass.

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    ์น˜์˜ํ•™๊ณผ/๋ฐ•์‚ฌ[ํ•œ๊ธ€] ๊ฒฝ์กฐ์ง ๊ฒฐ์†๋ถ€๋ฅผ ์ˆ˜๋ณตํ•˜๊ธฐ ์œ„ํ•ด ๋„๋ฆฌ ์‚ฌ์šฉ๋˜๊ณ  ์žˆ๋Š” ์žฌ๋ฃŒ ์ค‘ ์ƒ์ฒด๊ณจ์ด๋‚˜ ์น˜์•„์™€ ํ™”ํ•™์  ์กฐ์„ฑ์ด ์œ ์‚ฌํ•œ ์นผ์Š˜ ํฌ์ŠคํŽ˜์ดํŠธ ์„ธ๋ผ๋ฏน์€ ํก์ˆ˜ ์†๋„๊ฐ€ ๋Š๋ ค ๊ณจ๋กœ ๋Œ€์ฒด๋˜๊ธฐ๋ณด๋‹ค๋Š” ์ƒ์ฒด ๋‚ด ์ž”์กดํ•˜๊ฒŒ ๋˜๊ณ , ๊ทœ์‚ฐ์—ผ๊ณ„ ์ƒ์ฒด ํ™œ์„ฑํ™” ์œ ๋ฆฌ๋Š” ์กฐ์ง๊ณผ์˜ ๋ฐ˜์‘์„ฑ์ด ๋–จ์–ด์ง€๋ฏ€๋กœ, ์ƒ๋ถ„ํ•ด์„ฑ์ด ์šฐ์ˆ˜ํ•œ ์นผ์Š˜ ํฌ์ŠคํŽ˜์ดํŠธ ๊ธ€๋ผ์Šค๋ฅผ ์ œ์กฐํ•˜์—ฌ ์ƒ๋ฌผํ•™์ ์ธ ์„ฑ์งˆ์„ ํ‰๊ฐ€ํ•˜์˜€๋‹ค. CaO-CaFโ‚‚-Pโ‚‚O_5-MgO๊ณ„ ์นผ์Š˜ ํฌ์ŠคํŽ˜์ดํŠธ ๊ธ€๋ผ์Šค๋ฅผ ์ œ์กฐํ•œ ํ›„ X์„  ํšŒ์ ˆ ๋ถ„์„์œผ๋กœ ๊ธ€๋ผ์Šค์˜ ํ˜•์„ฑ์„ ํ™•์ธํ•˜์˜€๊ณ , ์„ฌ์œ ๋ชจ์„ธํฌ์ธ L929 ์„ธํฌ์™€ ์œ ์‚ฌ์ „๊ตฌ๊ณจ๋ชจ์„ธํฌ์ธ MC3T3-E1 ์„ธํฌ๋ฅผ ์ด์šฉํ•˜์—ฌ ๊ธ€๋ผ์Šค ์œ„์—์„œ ์„ธํฌ๋ฅผ ๋ฐฐ์–‘ํ•œ ํ›„ ์„ธํฌ์ˆ˜ ๋ฐ ๋‹จ๋ฐฑ์งˆ๋Ÿ‰ ์ธก์ •์„ ํ†ตํ•ด ์„ธํฌ ์ฆ์‹๋„๋ฅผ ์—ฐ๊ตฌํ•˜์˜€๊ณ , MC3T3-E1 ์„ธํฌ์˜ ์•Œ์นผ๋ฆฌ์„ฑ ์ธ์‚ฐํšจ์†Œ ํ™œ์„ฑ๋„์˜ ์ธก์ • ๋ฐ ๊ณจ๊ฒฐ์ ˆ ํ˜•์„ฑ์˜ ๊ด€์ฐฐ์„ ํ†ตํ•ด ๋ถ„ํ™”๋Šฅ์„ ์—ฐ๊ตฌํ•˜์˜€์œผ๋ฉฐ, ๋ฐฐ์–‘์•ก ๋‚ด ์นผ์Š˜ ๋†๋„์˜ ์ธก์ •์„ ํ†ตํ•ด ๊ณจ์„ธํฌ ๋ถ„ํ™”์™€์˜ ์—ฐ๊ด€์„ฑ์„ ์—ฐ๊ตฌํ•˜์—ฌ ๋‹ค์Œ๊ณผ ๊ฐ™์€ ๊ฒฐ๊ณผ๋ฅผ ์–ป์—ˆ๋‹ค. 1. L929 ์„ธํฌ ๋ฐ MC3T3-E1 ์„ธํฌ์˜ ์„ธํฌ ์ฆ์‹์œจ์€ ํด๋ฆฌ์Šคํ‹ฐ๋ Œ๊ตฐ๊ณผ ์นผ์Š˜ ํฌ์ŠคํŽ˜์ดํŠธ ๊ธ€๋ผ์Šค๊ตฐ๊ฐ„์˜ ์œ ์˜์ฐจ๊ฐ€ ์—†์—ˆ๋‹ค(p>0.05). 2. ์•Œ์นผ๋ฆฌ์„ฑ ์ธ์‚ฐํšจ์†Œ ํ™œ์„ฑ๋„๋ฅผ ์ธก์ •ํ•œ ๊ฒฐ๊ณผ, ๋ฐฐ์–‘ 10, 12, 14, 18์ผ ์นผ์Š˜ ํฌ์ŠคํŽ˜์ดํŠธ ๊ธ€๋ผ์Šค๊ตฐ์—์„œ ํด๋ฆฌ์Šคํ‹ฐ๋ Œ๊ตฐ๋ณด๋‹ค ์œ ์˜์„ฑ ์žˆ๊ฒŒ ๋†’์€ ํ™œ์„ฑ๋„๋ฅผ ๋ณด์˜€๋‹ค(p<0.05). 3. ๊ณจ๊ฒฐ์ ˆ ํ˜•์„ฑ์„ ๊ด€์ฐฐํ•œ ๊ฒฐ๊ณผ, ์นผ์Š˜ ํฌ์ŠคํŽ˜์ดํŠธ ๊ธ€๋ผ์Šค์—์„œ ๊ณจ๊ฒฐ์ ˆ์ด ํ˜•์„ฑ๋œ ์‹œ๊ธฐ๊ฐ€ ๋นจ๋ž์œผ๋ฉฐ, ๊ทธ ์–‘๋„ ๋งŽ์€ ๊ฒƒ์œผ๋กœ ๋‚˜ํƒ€๋‚ฌ๋‹ค. 4. ๋ฐฐ์–‘์•ก ๋‚ด ์นผ์Š˜๋Ÿ‰์„ ์ธก์ •ํ•œ ๊ฒฐ๊ณผ ์ „ ๊ธฐ๊ฐ„์„ ํ†ตํ•ด ์นผ์Š˜ ํฌ์ŠคํŽ˜์ดํŠธ ๊ธ€๋ผ์Šค๊ตฐ์ด ํด๋ฆฌ์Šคํ‹ฐ๋ Œ๊ตฐ๋ณด๋‹ค ์œ ์˜์„ฑ ์žˆ๊ฒŒ ๋งŽ์€ ์–‘์˜ ์นผ์Š˜์ด ์ธก์ •๋˜์—ˆ๋‹ค(p<0.05). ์ œ์กฐํ•œ ์นผ์Š˜ ํฌ์ŠคํŽ˜์ดํŠธ ๊ธ€๋ผ์Šค๋Š” ํด๋ฆฌ์Šคํ‹ฐ๋ Œ๊ณผ ์„ธํฌ ์ฆ์‹๋Šฅ์— ์ฐจ์ด๊ฐ€ ์—†์„ ๋ฟ ์•„๋‹ˆ๋ผ, ํด๋ฆฌ์Šคํ‹ฐ๋ Œ๋ณด๋‹ค MC3T3-E1 ์„ธํฌ์˜ ๋ถ„ํ™”๊ฐ€ ๋น ๋ฅธ ์‹œ์ผ์— ๋งŽ์ด ์œ ๋„๋˜์—ˆ๋‹ค. ์ด๋Š” ๋ฐฐ์–‘์•ก ๋‚ด๋กœ ์œ ๋ฆฌ๋˜๋Š” ์นผ์Š˜์˜ ๋†๋„์™€ ๊ด€๋ จ์ด ๋  ๊ฒƒ์œผ๋กœ ์ƒ๊ฐ๋˜์—ˆ์œผ๋ฉฐ, ๊ณจ๋Œ€์ฒด์žฌ๋กœ ์‚ฌ์šฉ์ด ๊ฐ€๋Šฅํ•  ๊ฒƒ์œผ๋กœ ์‚ฌ๋ฃŒ๋˜์—ˆ๋‹ค. [์˜๋ฌธ] The purpose of this study was to explore calcium phosphate glass as potential biomaterials for hard tissue repair. We prepared calcium phosphate glass in the system CaO-CaFโ‚‚-Pโ‚‚O_5-MgO and was characterized using X-ray diffraction. To evaluate the effects of calcium phosphate glass on growth and differentiation of L929 and MC3T3-E1 cells after culture for 6 and 21 days, respectively. Polystyrene was employed as a control specimen at that time. In order to estimate the cell proliferation activities, cell numbers and protein contents were determined. Alkaline phosphatase(ALP) activities as well as calcium contents in the culture medium were measured to determine the cell differentiation. Formation of calcified bone nodules was observed with Alizarin Red S and light green SF yellowish. The results were as follows: 1. There was no significant difference of cell proliferation between calcium phosphate glass and polystyrene group with L929 as well as MC3T3-E1 cells(p>0.05). 2. The alkaline phosphatase activity of calcium phosphate glass group exhibited greater significantly than that of polystyrene group after exceed 10 days(p<0.05). 3. The bone nodules began forming quickly and grew in abundance. 4. The resultant calcium content in the culture medium with the calcium phosphate glass group was higher significantly than that of polystyrene group(p<0.05). It can be seen that the prepared calcium phosphate glass exhibited almost same activity of cell proliferation, while much faster and abundant in the formation of bone nodule. The calcium phosphate glass could be expected to be useful in hard tissue repair.prohibitio

    Proliferation, differentiation, and calcification of preosteoblast-like MC3T3-E1 cells cultured onto noncrystalline calcium phosphate glass

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    The purpose of this study was to investigate calcium phosphate glass as a potential biomaterial for hard tissue repair. We prepared calcium phosphate glass using the system CaO-CaF(2)-P(2)O(5)-MgO-ZnO and cultured MC3T3-E1 cells onto the glass in alpha-MEM with beta-glycerophosphatase and ascorbic acid. Proliferation of the cells was determined to evaluate the biocompatibility of the prepared calcium phosphate glass. The alkaline phosphatase activity was measured to examine the osteoblast differentiation. Mineralization was evaluated by staining the calcium precipitates with Alizarin red. Culture onto the calcium phosphate glass exhibited no significant influence on cell proliferation compared to the polystyrene chosen as a control in this experiment (p > 0.05). The alkaline phosphatase activity in the experimental group, however, was enhanced by the calcium phosphate glass significantly at 10-18 days after incubation than that of the control group (p < 0.05). The promotion of bone-like tissue formation by the calcium phosphate glass was observed after 7 days and thereafter. The results of the present study indicate that the prepared calcium phosphate glass affects osteogenesis by increasing calcification of the extracellular matrix.ope
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