Studies on determination of glucosamine by HPLC-ELSD

目的:建立氨基葡萄糖含量测定的高效液相色谱分析法。方法:样品采用高效液相色谱进行测定,使用Waters Carbohydrate色谱柱,流动相为乙腈-水=70:30,用蒸发光散射检测器(ELSD)检测。结果:方法的进样量线性范围为2.0～12.0μg,相关系数为0.9997,回收率为96.0%～97.7%。结论:此法简便、快速、准确,无需衍生反应,是测定氨基葡萄糖含量的有效方法,并可用于硫酸氨基葡萄糖含量的测定。Objective:A HPLC method for the determination of glucosamine was developed.Methods:The content of glucosamine was determined by HPLC on a water carbohydrate column(4.6 mm×250 mm,5 μm) with acetonitrile-water(70:30) as mobile phase and ELSD detector.Results:The linear range of calibration curve was 2.0～12.0 μg(r=0.9997),the recovery was 96.0%～97.7%.Conclusion:The method is simple,rapid,accurate and without derivation.It is a effective method for determination of glucosamine and can be used for determination of glucosamine sulfate.厦门市重大科技项目(3502Z20051025

Studies on extraction of small molecule metabolites produced by marine microorganisms with macroporous adsorption resin

目的采用大孔吸附树脂建立海洋微生物小分子代谢产物库的粗提取方法。方法以吸附率、解吸率、蛋白与总糖吸附率、指纹图谱为评价目标,对常用的11种不同极性的大孔吸附树脂进行筛选。结果 dM11与Sd300串联吸附对发酵液中化合物的吸附率达7.528%,总糖与蛋白去除率分别为90.98%和77.69%,且此法几乎不损失乙酸乙酯萃取部分化合物。结论 dM11-Sd300串联树脂吸附法能快速有效地提取发酵液中的小分子化合物,为海洋微生物小分子代谢产物库的建立提供了实验基础。OBJECTIVE To establish a crude extraction method for the library of small molecule metabolites produced by marine microorganisms using macroporous adsorption resin( MAR).METHEDS 11 types of MARs were selected based on their adsorption rate,desorption rate,protein and polysaccharide adsorption rate.RESULTS DM11 and SD300 in series,were used to extrat small chemical molecules from bacterial fermentation liquid,by which the adsorption rate is up to 7.528%,while the removal rate of protein and polysaccharides is 90.98% and 77.69% respectively.In addition,this process barely lose compounds that extracted from ethyl acetate.CONCLUSION the approach of DM11-SD300 in series could extract small molecule metabolites from fermentation liquid rapidly and effectively,which will make a fundation to achieve the library of small molecule metabolites produced by marine microorganisms

中国海及邻近区域碳库与通量综合分析

中国海总面积约470万平方公里,纵跨热带、亚热带、温带、北温带等多个气候带.其中,南海北依\"世界第三极\"青藏高原、南邻\"全球气候引擎\"西太平洋暖池,东海拥有全球最宽的陆架之一,跨陆架物质运输显著,黄海是冷暖流交汇区域,渤海则是受人类活动高度影响的内湾浅海.中国海内有长江、黄河、珠江等大河输入,外邻全球两大西边界流之一的黑潮.这些鲜明的特色赋予了中国海碳储库和通量研究的典型代表意义.文章从不同海区（渤海、黄海、东海、南海）、不同界面（陆-海、海-气、水柱-沉积物、边缘海-大洋等）,以及不同生态系统（红树林、盐沼湿地、海草床、海藻养殖、珊瑚礁、水柱生态系统等）多层面对海洋碳库与通量进行了较系统地综合分析,初步估算了各个碳库的储量与不同碳库间的通量.就海气通量而言,渤海向大气中释放CO2约0.22Tg Ca-1,黄海吸收CO2约1.15Tg Ca-1,东海吸收CO2约6.92～23.30Tg Ca-1,南海释放CO2约13.86～33.60Tg Ca-1.如果仅考虑海-气界面的CO2交换,中国海总体上是大气CO2的\"源\",净释放量约6.01～9.33Tg Ca-1.这主要是由于河流输入以及邻近大洋输入所致.河流输入渤黄海、东海、南海的溶解无机碳（DIC）分别为5.04、14.60和40.14Tg Ca-1,而邻近大洋输入DIC更是高达144.81Tg Ca-1,远超中国海向大气释放的碳量.渤海、黄海、东海、南海的沉积有机碳通量分别为2.00、3.60、7.40、7.49Tg Ca-1.东海和南海向邻近大洋输送有机碳通量分别为15.25～36.70和43.39Tg Ca-1.就生态系统而言,中国沿海红树林、盐沼湿地、海草床有机碳埋藏通量为0.36Tg Ca-1,海草床溶解有机碳（DOC）输出通量为0.59Tg Ca-1;中国近海海藻养殖移出碳通量0.68Tg Ca-1,沉积和DOC释放通量分别为0.14和0.82Tg Ca-1.总计,中国海有机碳年输出通量为81.72～103.17Tg Ca-1.中国海的有机碳输出以DOC形式为主,东海向邻近大洋输出的DOC通量约15.00～35.00Tg Ca-1,南海输出约31.39Tg Ca-1.综上,尽管从海-气通量看中国海是大气CO2的\"源\",但考虑了河流、大洋输入、沉积输出以及微型生物碳泵（DOC转化输出）作用后,中国海是重要的储碳区.需要指出的是,文章数据是基于中国海各海区碳循环研究报道,鉴于不同研究方法上的差异,所得数据难免有一定的误差范围,亟待将来统一方法标准下的更多深入研究和分析.国家重点研发计划项目(编号:2016YFA0601400);;国家自然科学基金项目(批准号:91751207、91428308、41722603、41606153、41422603);;中央高校基础研究项目(编号:20720170107);;中海油项目(编号:CNOOC-KJ125FZDXM00TJ001-2014、CNOOCKJ125FZDXM00ZJ001-2014)资

Synthesis of magnetic, fluorescent and mesoporous core-shell-structured nanoparticles for imaging, targeting and photodynamic therapy

通讯作者地址: Chen, XL (通讯作者),Xiamen Univ, Coll Chem & Chem Engn, State Key Lab Phys Chem Solid Surfaces, Xiamen 361005, Peoples R ChinaA synthetic method to prepare novel multifunctional core-shell-structured mesoporous silica nanoparticles for simultaneous magnetic resonance (MR) and fluorescence imaging, cell targeting and photosensitization treatment has been developed. Superparamagnetic magnetite nanoparticles and fluorescent dyes are co-encapsulated inside nonporous silica nanoparticles as the core to provide dual-imaging capabilities (MR and optical). The photosensitizer molecules, tetra-substituted carboxyl aluminum phthalocyanine (AlC(4)Pc), are covalently linked to the mesoporous silica shell and exhibit excellent photo-oxidation efficiency. The surface modification of the core-shell silica nanoparticles with folic acid enhances the delivery of photosensitizers to the targeting cancer cells that overexpress the folate receptor, and thereby decreases their toxicity to the surrounding normal tissues. These unique advantages make the prepared multifunctional core-shell silica nanoparticles promising for cancer diagnosis and therapy.NSFC21021061,20925103,20871100,Fok Ying Tung Education Foundation 121011 NSF of Fujian Province 2009J06005 Fundamental Research Funds for the Central Universities 2010121015 Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministr

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对日照烟煤和京西无烟煤在不同温度下制得半焦进行了工业分析和硫的元素分析，研究了不同煤种制得半焦中挥发分、灰分、固定碳以及硫元素含量随制备温度不同的变化趋势，并在管式沉降炉中进行了半焦的燃烧实验，分别计算了不同半焦的燃烧动力学参数

Characteristics of Semi-Cokes Obtained from Coal Partial Gasification

对日照烟煤和京西无烟煤在不同温度下制得半焦进行了工业分析和硫的元素分析，研究了不同煤种制得半焦中挥发分、灰分、固定碳以及硫元素含量随制备温度不同的变化趋势，并在管式沉降炉中进行了半焦的燃烧实验，分别计算了不同半焦的燃烧动力学参数

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中低品位能源品位低,应用范围窄,并且还具有余热的产生与应用时间或地点的不匹配性等特点,导致其利用率较低。化学热泵是一种新型节能热泵技术,利用可逆化学反应的吸热和放热,具有热量提质、储能、增热以及冷冻功能,实现能源品位的提升与高效利用。文中在阐述化学热泵工作机理基础上,结合中低品位能源利用情况,重点介绍中低温化学热泵在储能、提升能量品位方面的研究进展