Generalized Shifts on Cartesian Products

It is proved that if E, F are infinite dimensional strictly convex Banach spaces totally incomparable in a restricted sense, then the Cartesian product E×F with the sum or sup norm does not admit a forward shift. As a corollary it is deduced that there are no backward or forward shifts on the Cartesian product`p1×`p2,1\u3c p16=p2\u3c∞, with the supremum norm thus settling a problem left open in Rajagopalan and Sundaresan in J. Analysis 7 (1999(, 75-81 and also a problem stated as unsolved in Rassias and Sundaresan

Construction of eukaryotic vector pcDNA3.1-flag-pygo2 and expression in C6 cell

目的构建pcDNA3.1-flag-pygo2真核表达载体并在C6细胞中进行表达。方法从小鼠脑胶质细胞中提取总RNA,RT-PCR法反转录合成c DNA,设计引物,调取目的片段,与pcDNA3.1-flag载体连接后转化大肠杆菌DH5α,LB平板筛选菌落,提取质粒。重组质粒pcDNA 3.1-flag-pygo2经过酶切鉴定及测序后,阳离子脂质体法转染C6细胞并经免疫细胞化学染色及蛋白印迹检测重组体的表达。结果重构质粒pcDNA 3.1-flag-pygo2经限制性核酸内切酶EcoRⅠ,HindⅢ酶切分析及测序检查,表明真核表达载体构建正确;瞬时转染C6细胞后,免疫细胞荧光染色及蛋白印迹检测表明转染细胞能够表达外源Pygo2基因。结论成功构建了pcDNA3.1-flag-pygo2真核表达载体并能够在真核细胞中进行表达,这为今后研究pygo2基因在胶质瘤中的作用机制奠定了基础。Objective To construct the eukaryotic expression vector pcDNA3.1-flag-pygo2 and express the combined protein in C6 cell line.Methods To extract total RNA from primary glial cell of mouse and to synthesize c DNA by RT-PCR,then design primer and clone whole segment of pygo2 gene.After the targeted gene was inserted into vector pcDNA3.1-flag,the recombined plamid was transformed into E coli DH5α for LB agar plate screening and the recombined plasmid were extracted and purified.After verification by double enzyme digestion and sequencing.,the constructed eukaryotic expression plamid was transfected to C6 cell line by lipofectamin method,finally the protein expression was detected by immunocytochemical staining as well as western blot.Results The new constructed vector was confirmed by restricted enzyme Eco R I,HindIII digestion assay and correct Pygo2 was verified by sequenceing.finally,pcDNA3.1-flag-hpygo2 can express exogenous pygo2 gene in glioma C6 cell line after transient transfection by the determination of immunocytofluorescent staining and western blot.Conclusion The new plamid pcDNA3.1-flag-pygo2 was constructed successfully,and can express fused protein in eukaryotic cell,which establish the foundation for future research on pygo2 gene function in human glioma

Property of Acrylic Composite Antifouling Coatings Based on Nano－ TiO2 Modified with KH－570

以硅烷偶联剂KH-570改性纳米TiO2,将其添加到丙烯酸树脂中,制得改性纳米TiO2复合丙烯酸树脂。通过扫描电镜表征和接触角分析,发现KH-570改性纳米TiO2复合丙烯酸树脂成膜后具有明显的微米-纳米表面结构,成膜物的水接触角由75&deg;提高到115&deg;;海上挂板实验表明:KH-570改性纳米TiO2复合丙烯酸防污涂料具有较强的防污性能,能够有效抑制海洋生物附着。;TiO2 nano－particles were modified with silane coupling agent ( KH－570) ，and then mixed with acrylic resin to form a nano－TiO2 composite acrylic resin． The scanning electron microscope ( SEM) and contact angle analyzer indicated that the nano－TiO2 composite acrylic resin could form a film showing obviously micro－ and /or nano－level surface structure and water contact angle increasing from 75&deg; to 115&deg;． The result derived from offshore field test indicated that the acrylic composite antifouling coatings based on nano－ TiO2 modified with KH－570 exhibited high antifouling property and effectively inhibited the adhesion of marine organisms．</p

内蒙古26种常见温带灌木的生物量模型

生物量模型是估算灌木生物量的重要方法之一,本文采用4种数学模型(一元线性模型、二元线性模型、对数模型、幂函数模型),3个预测变量株高(H)、冠幅(C)、植株体积(V)对内蒙古地区26种常见温带灌木进行生物量方程的拟合,同时比较不同生境类型间灌木根冠比的差异。结果表明:①最优生物量方程以幂函数模型和一元线性函数模型为主,最佳预测变量以冠幅(C)和植株体积(V)为主。②有17种灌木不同器官最优生物量方程的形式和预测变量相同,表明物种内的生物量方程形式具有一定的一致性;但各器官生物量方程的系数又各不相同,因此分种进行不同器官生物量的拟合可以更准确地估算生物量。③草地灌木和山地灌木的根冠比显著大于荒漠灌木的根冠比。通过建立分种分器官的生物量估算模型,可以为内蒙古地区灌木生物量的计算以及灌丛生态系统碳库的估算提供便利

Research progresses and prospects of microplastics in the environment

The term microplastics (MPs) refers to fine plastics less than 5 mm in size and includes primary sources from the original production of small-sized particles and secondary sources from the degradation or fragmentation of large plastics. MPs have been widely detected in marine (estuaries, bays, coastal zones, deep seas, ocean waters and sediments), soils (farmlands, urban soils, wetlands, landfill and polar areas), freshwater (lakes, rivers, reservoirs, snow and ice, and sewage treatment plants) and sediments, atmosphere (outdoor and indoor airs), living organisms (plants, animals, microorganisms, human and pet faeces), and foods (table salts, drinking waters, beers, vegetables and pet foods). Besides, MPs could be acted as the vector for many environmental pollutants, pathogens and even antibiotic resistance genes (ARGs). Moreover, MPs can be taken up by various terrestrial and aquatic organisms and transfer along the food chain at various trophic levels. Thus, environmental MP pollution is becoming one of the most serious threats to the Earth's surface ecosystems which has attracted serious concern and extensive research by many governments and the scientific community worldwide. Up to now, comprehensive studies and reports on the latest multidisciplinary research progress on microplastics in multiple environmental media remain limited. From the perspectives of earth sciences, chemistry, biology, and management, this article systematically reviews the research progress on the abundance, distribution and sources of microplastics in the waters, soils, atmosphere, sediments and organisms; the separation and analytical methods of microplastics in multiple environmental media; the migration and prediction of microplastics in terrestrial, marine and atmospheric environments; the surface changes and biofilm formation on microplastics and their adsorption characterization of environmental pollutants, pathogens and ARGs; the biological uptake, accumulation and ecological risks of MPs; the food chain transfer and health risks of MPs; the physico-chemical fragmentation and biodegradation of MPs in the environment and their risk reduction strategies and techniques. Finally, the key scientific issues and future research directions of environmental microplastics are also proposed in this review, such as methdology breakthroughs in separation and identification of submicron and nanoscale microplastics; comprehensive study on the distribution, migration, transport and flux of microplastics in environmental multi-media at cross-reginal and global scale; comprehensive monitoring, quantitative characterization and long-term evaluating the impacts of environmental microplastics on ecosystems; and the systematic assessment of human health risks of microplastics to different populations