Experimental Study on the Effect of Sanchong Banxia Baizhu Tianma Decoction on the Expression of 5-HT1B/1D Receptor in the Brain of Rats with Migraine

目的探索三虫半夏白术天麻汤治疗偏头痛的作用机制。方法建立硝酸甘油致大鼠偏头痛模型,给予半夏白术天麻汤高、中、低剂量灌胃干预7 d,以苯甲酸利扎曲普坦为西药阳性对照药物,并予空白组、模型组进行对照。运用RT-PCR检测5羟色胺1B、1D(5-HT1B/1D)受体的基因表达。结果 1)对5-HT1B受体表达的影响:与空白组相比,模型组显著低于空白组(P0.05),中药中剂量组、中药高剂量组、西药组及联合用药组显著低于空白组(P0.05)。2)对5-HT1D受体表达的影响:与空白组相比,中药中剂量组显著降低(P0.05);与模型组相比,各组无显著差异(P>0.05);与中药中剂量组相比,空白组、中药低剂量组、西药组、联合用药组较中药中剂量组显著升高(P0.05)。结论 5-HT1B/1D受体调节异常为偏头痛的发病环节之一。三虫半夏白术天麻汤可以提高偏头痛大鼠脑组织5-HT1B受体的基因表达,对5-HT1D受体的基因表达具有双向调节作用,临床运用,剂量不宜过大。Objective: To explore the mechanism of Sanchong Banxia Baizhu Tianma Decoction in the treatment of migraine. Methods: Migraine model of SD rats were set up with nitroglycerin,given Banxiabaizhutianma decoction of high,medium and low-dose intervention respectively for 7 days,taking Rizatriptan Monobenzoate as a positive control drug medicine,compared with the blank control group and the model group. RT-PCR was used to test the genetic expression of the 5-HT1 B receptor and the 5-HT1 D receptor. Results: The influence on the expression of the 5-HT1 B receptor: compared with the blank control group,the model group was significantly lower than the control group(P 0.05);traditional Chinese medicine medium dose group,high dose group,western medicine group and the combination group were significantly lower than the blank control group(P 0.05). The influence on the expression of 5-HT1 D receptor:compared with the blank control group,traditional Chinese medicine medium dose group decreased significantly(P 0.05);compared with the model group,there was no significant differ ence among them(P > 0.05);compared with the traditional Chinese medicine medium dose group,the blank control group,traditional Chinese medicine low dose group,western medicine group and combination group were significantly higher(P 0.05). Conclusion: 5-HT1B/1D receptor dysregulation is one cause of the pathogenesis of migraine. Sanchong banxia baizhu tianma decoction can improve the genetic expression of 5-HT1 B receptors in migraine rats′ brains,and has a dual-direction regulation on genetic expression of the 5-HT1 D receptors. In the field of clinical application,the dose should not be too large.2012福建省中医药科研课题(wst201211

Experimental Study on the Basic Effect of Huatan Anshen Decoction and on Orexin Expression in Hypothalamus of Insomnia Rats

目的：研究化痰安神方治疗失眠症的药效学基础及其对失眠大鼠下丘脑食欲素表达的影响,揭示本方部分药效学机制。方法：通过小鼠自发活动实验、戊巴比妥钠阈下及阈上睡眠协同实验探索化痰安神方治疗失眠症的药效学基础;通过对氯苯丙氨酸（PCPA）制造失眠大鼠模型,分为中药组、西药组、空白组、模型组,每组干预7天,使用免疫组化检测实验大鼠下丘脑食欲素（Orexin）。结果：（1）化痰安神方可抑制小鼠自发活动,与空白组比较,具有显著差异（P〈0.01）;（2）对戊巴比妥钠阈下入睡率的影响：中药组、对照组、西药组实验小鼠入睡率分别为：65%、72%和100%,西药组入睡率明显高于中药组、对照组（P〈0.01）;睡眠持续时间,中药组长于西药组（P〉0.05）,西药组长于对照组（P〈0.05）;（3）对戊巴比妥钠阈上睡眠情况的影响：中药组睡眠持续时间明显长于西药组（P〈0.01）,西药组长于对照组（P〈0.01）;（4）对氯苯丙氨酸（PCPA）致失眠大鼠各组下丘脑食欲素表达水平无差异（P〉0.05）。结论：化痰安神方具有一定的镇静催眠作用,可明显延长失眠患者的持续睡眠时间,其作用机理可能不是通过调节食欲素水平这一途径。Objective： To study the basic effect of Huatan Anshen decoction and the effect on orexin expressions in Hypothalamus of insomnia rats and reveal the partial pharmacodynamics mechanism. Methods： Spontaneous activity in mice and synergistic action test of pentobarbital sodium were observed to study the basic effect of Huatan Anshen decoction. The second part, through para chlorophenylalanine （PCPA） making rat model of insomnia, divided into Chinese medicine group and Western medicine group, blank group, model group, each group of rats were intervened for 7 days, Hypothalamus Orexin was detected by immunohistochemisty. Results： （1） Huatan Anshen Decoction can inhibit the spontaneous activity of mice, compared with the control group, with significant difference （P〈0.01）. （2） Effect on the rate of sleep pentobarbital sodium： Chinese medicine group and control group, western medicine group mice sleep rates were： 65%, 72% and 100%. The rate of western medicine group was significantly higher than that of traditional Chinese medicine group and control group （P〈0.01）. Sleep duration： Chinese medicine group was longer than the western medicine group （P〈0.05）, the western medicine group was longer than the control group （P〈0.05）. （3）Sleep duration of Chinese medicine group was significantly longer than the western medicine group （P〈0.01）, and the western medicine group was significantly longer than the control group（P〈0.01）. （4） There was no difference in the expression level of orexin （P〈0.05） in the hypothalamus of insomnia rats induced by phenylalanine （PCPA）. Conclusion： Huatan Anshen decoction has sedative and hypnotic effects, can prolong the duration of sleep in patients withinsomnia. The mechanism of Huatan Anshen decoction to prolong the duration of sleep in patients with insomnia may not be regulated by the level of orexin pathways.2013福建省中医药科研课题（批准号：wzln201311

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel