Studies on degradation mechanism of niclosamide ethanolamine salt Ⅰ Determination of niclosamide ethanolamine salt in photolysis solution

目的了解氯硝柳胺乙醇胺盐(nES)在水体中的光解规律。方法实验室配置PH5的0.05μg/Ml、PH7的0.5μg/Ml和PH9的2.5μg/MlnES溶液,采用氙灯光源作为模拟日光照射,照射后0、0.5、1、2、4、8、16、24、48、72、96H采集光解液,高效液相色谱法测定光解液中nES含量,同时设无光照对照试验,计算其半衰期。结果在氙灯光光照条件下,nES快速降解,8H3种PH值的nES溶液下降率分别为83.4%、78.8%和41.3%,24H下降率分别为92.1%、88.5%和95.8%。nESPH5半衰期为8.98H,PH7为10.34H,PH9为9.16H;无光照条件下放置12d溶液浓度稳定。结论nES可在水中快速光解。Objective To understand the photolysis mechanism of niclosamide ethanolamine salt(NES) in water body.Methods NES was formulated into solutions of pH 5(0.05 μg/ml),7(0.5 μg/ml) and 9(2.5 μg/ml),respectively.The xenon gas lamp was used as the simulated sunlight.After 0,0.5,1,2,4,8,16,24,48,72,96 h of the illumination,the photolysis solutions were collected to determine the amount of NES by using the high-performance liquid chromatography method(HPLC),and the experiment without illumination was also set as the control.The half-life was calculated.Results Under the illumination of xenon gas lamp,the NES degraded rapidly.The degradation rates were 83.4%,78.8% and 41.3%,respectively for 8 h,and 92.1%,88.5% and 95.8%,respectively for 24 h.The half-life of NES was 8.98 h for pH 5,10.34 h for pH 7,9.16 h for pH 9.The solution could keep stable for 12 d without illumination.Conclusion Photolysis of NES can occur quickly in water.江苏省自然科学基金(BK200602

Studies on degradation mechanism of niclosamide ethanolamine salt Ⅱ Isotopic tracing and determination of photolysis end-product

目的观察同位素示踪技术解析氯硝柳胺乙醇胺盐(nES)在水体中的稳定性及测定光解终产物CO2的变化。方法实验室配制PH5的0.05μg/Ml、PH7的0.5μg/Ml和PH9的2.5μg/MlnES溶液,采用氙灯光源作为模拟日光照射上述nES溶液,照射8、16、24、32、40、48H和72H后,分别测定nES溶液在光解管顶的气态样品,采用gASbEnCHⅡ进样针插入直接分析;液体样品则取2.5Ml注入用高纯氦吹过的密封顶空样品瓶中,用加酸泵滴加无水磷酸,30℃超声反应1H,再插入gASbEnCHⅡ进样针进行分析;与dElTAPluS/XP稳定同位素质谱仪联用测定光解产物CO2的量及碳稳定同位素。结果在氙灯光光照条件下,PH70.5μg/Ml和PH92.5μg/MlnES溶液的光解,随光照时间的增加,CO2的量也增加,并呈线性增长,72H时其产量接近nES的实际产量。同位素结果显示,随光照时间的增加,CO2的碳同位素值逐渐变负,72H时接近nES原药的碳同位素值,为-25.36±0.11,光降解已接近完全。而PH50.05μg/MlnES溶液的光解,随光照时间的增加,CO2的量也在增加,但其产量超出了nES的实际产量;同位素结果显示,随光照时间的增加,产出CO2的碳同位素值逐渐变负,但在光照16H后其产CO2的碳同位素值比nES的碳同位素值偏负。结论 nES能够在水中快速光解,终产物为CO2。Objective To analyze the stability of niclosamide ethanolamine salt(NES)in water and determine the changes of CO2,the photolysis end-product,by using the isotopic tracing technique.Methods NES was formulated into the solutions of pH 5(0.05 μg/ml),pH 7(0.5 μg/ml)and pH 9(2.5 μg/ml),respectively.The xenon gas lamp was used as the simulated sunlight.After 8,16,24,32,40,48,72 h of the illumination,the gas samples of the NES solution on the top of photolysis tube were determined by using direct insertion of GasBenchII needle.For liquid samples,2.5 ml NES solution was added into a sealed empty bottle,which was treated with high pure helium,and then anhydrous phosphoric acid was added.After ultrasonic reaction at 30 ℃ for 1 h,the GasBenchII needle was inserted for analysis.The amount of CO2 and carbon isotopic value were determined by using the GasBenchII needle combined with isotopic mass spectrometer.Results Under the illumination of xenon gas lamp,the NES solutions of pH 7 and pH 9 occurred photolysis,with the increase of illumination time,the amount of CO2 increased,showing a linear increase.At 72 h,its yield approximated to the active output of NES.The isotopic tracing results showed,with the increase of illumination time,the carbon isotope value of CO2 turned to be negative gradually,and approximately reached to the carbon isotope value(-25.36±0.11‰)of NES active ingredient at 72 h when the photolysis approximated to be completely finished.For the photolysis of pH 5 NES solution,with the increase of illumination time,the amount of CO2 increased,however,its output was more than the active output of NES.The isotopic tracing results showed,with the increase of illumination time,the carbon isotope value of CO2 turned to be negative gradually.However,after illumination for 16 h,the carbon isotope value of CO2 was more negative than that of NES.Conclusions The photolysis of NES can occur rapidly in water,and the end product is CO2.江苏省自然科学基金(BK200602);江苏省卫生厅项目(X200504

Immunological Recognition of Gonadotropin-releasing Hormone Receptor(GnRH-R) in the Mud Crab,Scylla paramamosain

促性腺激素释放激素(gnrH)及其受体(gnrH-r)对生殖具有重要的调控作用.迄今无脊椎动物gnrH及gnrH-r的研究尚少.本研究采用兔抗人gnrH-r、兔抗果蝇gnrH-r和兔抗海鞘gnrH-r的抗体,应用免疫印迹和免疫共沉淀技术对拟穴青蟹(SCyllAPArAMAMOSAIn)gnrH-r进行了免疫识别,所得结果如下:1)经免疫印迹检测,拟穴青蟹脑、胸神经团、视神经节和精巢中共有的免疫阳性条带分子质量为45--55 ku.脑和胸神经团中另有一条36 ku条带;在精巢中,兔抗人gnrH-r和兔抗果蝇gnrH-r抗体只有一条28 ku条带,而兔抗海鞘gnrH-r抗体显示有28,36和30 ku的条带.2)利用免疫共沉淀技术,分离出与gnrH-r抗体相结合的两种物质,分子质量分别为38.1和54.0 ku,分别与哺乳动物和非哺乳动物gnrH-r的分子质量接近.该发现提示拟穴青蟹体内存在gnrH-r,为深入了解甲壳动物生殖调控机理提供了理论依据.Gonadotropin-releasing hormone(GnRH) and its receptor(GnRH-R)play an important role in the regulation of reproduction.To date,there has been little study on the GnRH and GnRH-R in invertebrates.In this study,with three GnRH-Rs of rabbit antibody against different species,including human,fruit fly and sea squirt,Western blotting and co-immunoprecipitation were applied to test the GnRH-R in mud crab,Scylla paramamosain.The common immunoreactive bands,with the molecular mass between 45 and 55 ku,appeared in the brain,thoracic ganglion,eyetalk and testes.Another band with 36 ku was found in the brain and thoracic ganglion.And in the testes,there was only a band with 28 ku using the antibody against human and fruit fly,while using rabbit antibody against sea squirt,there were bands with 28,36 and 30 ku.Two substance combined to GnRH-R were separated by co-immunoprecipitation,with the molecular mass of 38.1 and 54.0 ku,which were close to the mammal and non-mammal,respectively.This study shows that GnRH-R occurs in mud crab,which is helpful to further understanding of the regulation of reproduction in crustaceans.国家自然科学基金项目(41076081

新型电荷型纳米盘的可控制备及其与细胞色素p450的结合性能

分别采用氮气吹干法和旋转蒸发法制备由磷脂和膜支架蛋白组成的电荷型纳米盘,用凝胶过滤色谱对其尺寸分级,分析了其性能,考察了其与肝微粒体细胞色素P450的结合能力。结果表明,纳米盘外观澄清透明,微观呈圆盘状,平均直径10 nm,在pH 7.4下Zeta电位为-19.86 mV;肝微粒体破碎液与纳米盘能很好结合,CO差示光谱在450 nm出现明显吸收峰,细胞色素P450含量为0.10 nmol/mg,比活比未经纳米盘处理时提高13.0倍,较传统方法提升1.5倍,且操作时间由数日缩短至数小时。电荷型纳米盘在结合膜蛋白细胞色素P450的同时,活性保持良好,在膜蛋白研究领域极具应用潜力

~(22)Na(p,γ)~(23)Mg反应的实验研究

讨论了目前有关2 2 Na(p ,γ) 2 3Mg反应的实验研究工作 ,结合兰州放射性束流线上的放射性束流2 3Al的β+延发质子衰变实验的测量结果 ,给出了2 3Al延发衰变的质子能谱 ,并比较了近期实验给出的相关能级的自旋、宇称值 ,正是由于这种自旋、宇称和能级部分宽度的不确定性 ,导致了反应率计算的不确定性 .计算了同位旋相似态的共振强度 .对于测量到的新的延发衰变能级Ed =8.91 6MeV ,由于没有相应的能级宽度值 ,实验仅给出其相对共振强度

~(20)Na的β~+延发α粒子测量

利用兰州放射性次级束流线提供的2 0 Na束流 ,通过2 0 Na β+2 0 Ne →16 O+α过程 ,测量了2 0 Na的衰变半衰期T1/2 及衰变α粒子能谱 .结果表明 ,除了Ed≥ 2 .688MeV的 9条较高激发能级的衰变α粒子外 ,实验中还观察到衰变能量Ed 为 0 .890和 1 .0 54MeV ,1 .991MeV ,2 .4 2 4和 2 .4 57MeV的2 0 Ne低激发能级的 3条α谱线

~(23)Al 的β~+延发质子测量

利用ＴＯＦ－ Ｅ和０°注入探测器的方法，鉴别并测量了２３Ａｌβ＋延发质子衰变能谱，通过精密脉冲发生器和计数器测得２３Ａｌ的半衰期 Ｔ１／２＝（４７６±４５）ｍｓ．实验中重现了能量为０．２１６，０．２７８，０．４３８，０．４７９ＭｅＶ的低能衰变质子．另外，还观察到了一个新的β＋延发衰变能级 Ｅｘ＝ ８．９１６ＭｅＶ，并给出了它们的相对强度

RIBLL上的β~+缓发粒子发射的在束测量

报道了在新建成的放射性次级束流线上完成的2 0 Na的 β+缓发α粒子发射2 0 Na—→β+　 　2 0　Ne →16 O +α的在束测量 .通过飞行时间和能损符合的方法实现2 0 Na次级束流的在束鉴别与调制 .在束和停束两个获取时段分别完成对次级束流和β+缓发粒子的记录 .利用脉冲发生器和记数器实现2 0 Na缓发粒子衰变半衰期的测量 .实验测量到2 0 Ne几个低能共振能级的衰变能量分别为 2 69,3 0 9,4 74 ,5 54MeV ,相对强度分别为 1 0 0 ,4 1 5,1 1 0 ,1 5 2 0 .测量到2 0 Na的β+缓发α衰变的半衰期为 (4 59± 7)ms,与现有的核数据基本上吻合

β~+延发粒子衰变的一种在束测量方法

报道了一种在束测量β+ 延发粒子的方法。由新建成的兰州放射性次级束流线 (RIBLL )提供的2 0 Na次级束 ,利用飞行时间 (TOF)和能损 (ΔE)符合的方法实现 2 0 Na次级束流的在束鉴别与调制。将数据获取过程分为有束和停束两个获取时段 ,分别完成对次级束流和β+ 延发粒子的记录。同时利用脉冲发生器和计数器实现2 0 Na延发粒子衰变半衰期的测