Studies on degradation mechanism of niclosamide ethanolamine salt Ⅰ Determination of niclosamide ethanolamine salt in photolysis solution

目的了解氯硝柳胺乙醇胺盐(nES)在水体中的光解规律。方法实验室配置PH5的0.05μg/Ml、PH7的0.5μg/Ml和PH9的2.5μg/MlnES溶液,采用氙灯光源作为模拟日光照射,照射后0、0.5、1、2、4、8、16、24、48、72、96H采集光解液,高效液相色谱法测定光解液中nES含量,同时设无光照对照试验,计算其半衰期。结果在氙灯光光照条件下,nES快速降解,8H3种PH值的nES溶液下降率分别为83.4%、78.8%和41.3%,24H下降率分别为92.1%、88.5%和95.8%。nESPH5半衰期为8.98H,PH7为10.34H,PH9为9.16H;无光照条件下放置12d溶液浓度稳定。结论nES可在水中快速光解。Objective To understand the photolysis mechanism of niclosamide ethanolamine salt(NES) in water body.Methods NES was formulated into solutions of pH 5(0.05 μg/ml),7(0.5 μg/ml) and 9(2.5 μg/ml),respectively.The xenon gas lamp was used as the simulated sunlight.After 0,0.5,1,2,4,8,16,24,48,72,96 h of the illumination,the photolysis solutions were collected to determine the amount of NES by using the high-performance liquid chromatography method(HPLC),and the experiment without illumination was also set as the control.The half-life was calculated.Results Under the illumination of xenon gas lamp,the NES degraded rapidly.The degradation rates were 83.4%,78.8% and 41.3%,respectively for 8 h,and 92.1%,88.5% and 95.8%,respectively for 24 h.The half-life of NES was 8.98 h for pH 5,10.34 h for pH 7,9.16 h for pH 9.The solution could keep stable for 12 d without illumination.Conclusion Photolysis of NES can occur quickly in water.江苏省自然科学基金(BK200602

Studies on degradation mechanism of niclosamide ethanolamine salt Ⅱ Isotopic tracing and determination of photolysis end-product

目的观察同位素示踪技术解析氯硝柳胺乙醇胺盐(nES)在水体中的稳定性及测定光解终产物CO2的变化。方法实验室配制PH5的0.05μg/Ml、PH7的0.5μg/Ml和PH9的2.5μg/MlnES溶液,采用氙灯光源作为模拟日光照射上述nES溶液,照射8、16、24、32、40、48H和72H后,分别测定nES溶液在光解管顶的气态样品,采用gASbEnCHⅡ进样针插入直接分析;液体样品则取2.5Ml注入用高纯氦吹过的密封顶空样品瓶中,用加酸泵滴加无水磷酸,30℃超声反应1H,再插入gASbEnCHⅡ进样针进行分析;与dElTAPluS/XP稳定同位素质谱仪联用测定光解产物CO2的量及碳稳定同位素。结果在氙灯光光照条件下,PH70.5μg/Ml和PH92.5μg/MlnES溶液的光解,随光照时间的增加,CO2的量也增加,并呈线性增长,72H时其产量接近nES的实际产量。同位素结果显示,随光照时间的增加,CO2的碳同位素值逐渐变负,72H时接近nES原药的碳同位素值,为-25.36±0.11,光降解已接近完全。而PH50.05μg/MlnES溶液的光解,随光照时间的增加,CO2的量也在增加,但其产量超出了nES的实际产量;同位素结果显示,随光照时间的增加,产出CO2的碳同位素值逐渐变负,但在光照16H后其产CO2的碳同位素值比nES的碳同位素值偏负。结论 nES能够在水中快速光解,终产物为CO2。Objective To analyze the stability of niclosamide ethanolamine salt(NES)in water and determine the changes of CO2,the photolysis end-product,by using the isotopic tracing technique.Methods NES was formulated into the solutions of pH 5(0.05 μg/ml),pH 7(0.5 μg/ml)and pH 9(2.5 μg/ml),respectively.The xenon gas lamp was used as the simulated sunlight.After 8,16,24,32,40,48,72 h of the illumination,the gas samples of the NES solution on the top of photolysis tube were determined by using direct insertion of GasBenchII needle.For liquid samples,2.5 ml NES solution was added into a sealed empty bottle,which was treated with high pure helium,and then anhydrous phosphoric acid was added.After ultrasonic reaction at 30 ℃ for 1 h,the GasBenchII needle was inserted for analysis.The amount of CO2 and carbon isotopic value were determined by using the GasBenchII needle combined with isotopic mass spectrometer.Results Under the illumination of xenon gas lamp,the NES solutions of pH 7 and pH 9 occurred photolysis,with the increase of illumination time,the amount of CO2 increased,showing a linear increase.At 72 h,its yield approximated to the active output of NES.The isotopic tracing results showed,with the increase of illumination time,the carbon isotope value of CO2 turned to be negative gradually,and approximately reached to the carbon isotope value(-25.36±0.11‰)of NES active ingredient at 72 h when the photolysis approximated to be completely finished.For the photolysis of pH 5 NES solution,with the increase of illumination time,the amount of CO2 increased,however,its output was more than the active output of NES.The isotopic tracing results showed,with the increase of illumination time,the carbon isotope value of CO2 turned to be negative gradually.However,after illumination for 16 h,the carbon isotope value of CO2 was more negative than that of NES.Conclusions The photolysis of NES can occur rapidly in water,and the end product is CO2.江苏省自然科学基金(BK200602);江苏省卫生厅项目(X200504

Preparation and biological safety of basic fibroblast growth factor/double-layered collagen composite

背景:胶原特殊的分子结构和生物活性有利于多种细胞黏附、增殖和分化,并可降解为新生组织提供足够空间。目的:制备一种复合负载碱性成纤维细胞生长因子的壳聚糖-肝素纳米粒子双层胶原基复合材料,并评价其生物安全性。方法:制备交联风干胶原膜和交联冻干胶原膜。将壳聚糖-肝素纳米粒滴于交联冻干胶原膜上,再将湿态交联风干胶原膜置于复合纳米粒子的交联冻干胶原膜上风干,即碱性成纤维细胞生长因子/双层胶原基复合材料。采用急性全身毒性试验、溶血试验、热原试验和细胞毒性试验评价其生物安全性。结果与结论:碱性成纤维细胞生长因子/双层胶原基复合材料为双层结构,一侧表面致密,另一侧疏松多孔。在其中间负载碱性成纤维细胞生长因子的壳聚糖-肝素纳米粒子呈不规则球形分布于胶原膜内侧面;急性全身毒性试验、热原试验、溶血试验均为阴性,细胞毒性为0级。说明碱性成纤维细胞生长因子/双层胶原基复合材料具有良好的生物安全性,对机体无毒,符合ISO10993-1评价标准。BACKGROUND: Collagen is a common used scaffold in tissue engineering, its specific molecular structure and biological activity are conducive to a variety of cell adhesion, proliferation and differentiation, and can speed up wound healing and degradation to provide sufficient space for new tissues.OBJECTIVE: To prepare a kind of inhomogeneous double layered collagen composite incorporated with basic fibroblast growth factor (bFGF) loaded chitosan-heparin (CS-Hep) nanoparticles, and to evaluate its biological safety.METHODS: The air-dried dense layer collagen membranes and freeze-dried loose layer collagen membrane were prepared respectively and then cross-linked by D-ribose (termed as CAM and CFM respectively).CS-Hep nanoparticles were prepared and then dropped on the surface of CFM.The wet CAM was laid on the surface of CFM with nanoparticles, and then air dried completely at 4 ℃.The obtained material was termed as bDM.Its physical and chemical properties were investigated.In addition, its bio-safety was also examined through acute systemic toxicity, pyrogen, hemolysis and cellular toxicity tests.RESULTS AND CONCLUSION: The bFGF/double-layered collagen composite was made up of double layers with one dense layer (CAM) and another loose layer (CFM).As part of bDM, CS-Hep nanopartiles with irregular global shape were aligned in the scope of D-period structure of the inner surface of CFM; the bFGF/double-layered collagen composite had no systemic acute toxicity, no cytotoxicity and pyrogen reactions as well as no hemolytic effect.The bFGF/double-layered collagen composite is prepared and found to have good biocompatibility and safety.Moreover, this material conforms to the ISO 10993-1, and can be used as a basic scaffold material in tissue engineering天津市科技支撑计划重点项目(08ZCKFSF02100);博士点基金项目(20101106110042)---

鳜鱼弹状病毒的分离、鉴定及基因组测序分析

在证实球形和弹状两种鳜鱼病毒共感染引起宿主细胞超微变化的基础上，利用电镜技术，辅助进行病毒挑斑分离，围绕鳜鱼弹状病毒的鉴定、全基因组测序及功能基因表达、分析等开展研究。主要研究方法和结果如下： 1、经7 轮挑斑，从共感染的两种病毒中，分离出一端为圆锥体、另一端为平底的典型子弹状形态，大小约为76-118 nm X 29-52 nm 的SCRV。 2、细胞系敏感性测定结果表明，GCF、FHM和EPC细胞对SCRV敏感，CHSE-214和RTG-2对SCRV不敏感。理化性质测定表明，SCRV 对氯仿、温度和pH 敏感，在46℃水浴1 小时后病毒完全灭活；当在pH 低于3 或高于10 的环境中，SCRV 也彻底灭活。 3、SDS-PAGE 电泳显示，SCRV 有五条主要蛋白带；琼脂糖凝胶电泳显示，SCRV基因组RNA大学约为11kb。 4、对SCRV 全基因组序列进行了克隆和分析。SCRV基因组全长为11545 个核苷酸。五个基因及非编码区的结构排列为：3’ Leader-N-P-M-G-L-Trailer5’。其中M 基因含有两个独立的互不重叠的开放阅读框（open reading frame, ORF），大的ORF 编码M 蛋白，小的ORF 编码一个5.4 kDa 的未知蛋白（暂称为Ms），而Ms 在已知的所有弹状病毒基因组中都不存在。氨基酸序列比对结果显示：SCRV N、P、M 和G 蛋白均与鱼类弹状病毒903/87相应蛋白的同源性最高，分别为41.5％、14.8％、25.8％和25.3％。在903/87 L 蛋白尚未测出全序列的情况下，SCRV L 蛋白显示与人类一种弹状病毒（Chandipura virus，CHPV）L 蛋白同源性最高，为44.7％。分别构建五个基因的遗传进化树，结果显示SCRV N、P、M 和G 基因均与903/87 相应基因聚为一支，L 基因则与SVCV L 基因聚为一支。 5、分别对SCRV N 与M 基因进行了原核表达，获得了大小分别约为73和38kDa的融合蛋白，并制备了相应蛋白的抗体。所制备的抗体能够特异性识别SCRV 的N 和M 蛋白。采用免疫荧光技术对N 和M 蛋白细胞内的分布进行了检测，结果表明，病毒在细胞内复制时，N 和M 蛋白分别分布于胞质中及胞膜周围。另外，还测定了兔抗SCRV N 蛋白血清的体外抗病毒活性，显示一定时间内，抗血清具有抑制SCRV 在GCF 细胞中复制的效果

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管理是一个社会过程，企业管理应该强调以人为中心，而人的行为无不受文 化环境的影响，所以企业管理可以通过培育与塑造优秀的企业文化来实施对人的 管理。但是我国传统的社会文化及企业文化中存在着种种缺陷与不足，制约与阻 碍着我国的经济和企业的发展，已经不适合社会发展的要求了，社会主义市场经 济体制的建立呼唤着新型的企业文化模式。本文正是本着这一目的和要求，依据 “古为今用，洋为中用”的原则，借鉴西方市场经济启动和发展的文化基础，探索我 国企业文化建设的新路子。 第一章管理是一个社会过程，所以应该重视处理企业内外人与人之间的关 系问题。当代管理主题是企业文化，企业文化在管理中可以起到导向...学位：工商管理硕士院系专业：管理学院工商管理教育中心_工商管理硕士(MBA)学号：19941502

鳜鱼弹状病毒毒株及制备方法和应用

本发明公开了一种鳜鱼弹状病毒毒株及制备方法和应用，鳜鱼弹状病毒毒株Siniperca chuatsi rhabdovirus，SCRV0208，CCTCC NO：V202008，首先是GCF0208细胞经TC199培养基加牛血清，培养一定时间，长成单层细胞，用病毒液接种后，不同时段取样，切片，通过电镜观察确定挑斑时间，挑斑后再接种到单层细胞中，扩大培养，离心后获得提纯的病毒。本发明方法易行，有效地将弹状病毒与之伴随感染的病毒分开，获得高纯度的病毒抗原，在细胞工程疫苗和基因工程疫苗中的应用

Development of Passive Infrared Detector Applied in Solar Lighting System

【中文摘要】 给出了一种应用于太阳能系列产品的新型人体红外信号探测系统的研制方法,该方法将探测到的人体红外信号进行滤波放大后驱动相应负载工作,同时利用自行研制的芯片IV0201取代LM324组成滤波放大电路并加入IV0103、IV0104两颗智能型电源管理芯片,从而使该系统成功的用在太阳能系列产品,并可满足低成本、高可靠性、低功耗等要求。 【英文摘要】 A method is proposed for developing an innovative infrared human signal detection system applied in solar energy products.It can detect human infrared signals and enlarge them to drive the load efficiently with an infrared signal filter.By the use of a self-developed chip IV0202 to replace LM324,a new amplified-filter circuit is formed and two intelligent power management chips IV0103 and IV0104 are included in the circuit so as to apply the system to solar energy products to satisfy the requirements for lo...福建省重大科技项目资助(2006H0092

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于2009年4月19日~5月17日在成都城区每天采集PM2.5样品.利用热光碳分析仪、离子色谱、X荧光光谱仪和高效阴离子交换色谱分别分析样品中有机碳/元素碳、水溶性离子、地壳元素和左旋葡聚糖,同步测量了大气散射系数(bsp)和气象数据.利用IMPROVE方程重建大气散射系数,并与实测大气散射系数进行对比.结果发现,PM2.5浓度均值为133.2&mu;g.m-3,大气散射系数为530 Mm-1.左旋葡聚糖和地壳元素能很好地反映生物质燃烧和沙尘事件.观测期间成都城区计算值b&rsquo;sp为504 Mm-1,(NH4)2SO4、NH4NO3、OM(organic matter)、FS(fine soil)和CM(coarse mass)贡献率分别为26%、15%、53%、4%和2%.DS(dust storm)期间,计算值b&rsquo;sp为575 Mm-1,FS和CM贡献率达到17%和21%.BB(biomass burning)期间,计算值b&rsquo;sp为635 Mm-1,OM贡献率达到62%.</p